DOI QR코드

DOI QR Code

Isolation and Characterization of Pepper mottle virus Infecting Tomato in Korea

  • Kim, Mi-Kyeong (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kwak, Hae-Ryun (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Han, Jung-Heon (Pepper and Breeding Institute) ;
  • Ko, Sug-Ju (Cucumber Experiment Station, Jeonnam Agricultural Research and Extension Services) ;
  • Lee, Su-Heon (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Park, Jin-Woo (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Jonson, Miranda Gilda (Department of Agricultural Biotechnology, Seoul National Unviersity) ;
  • Kim, Kook-Hyung (Department of Agricultural Biotechnology, Seoul National Unviersity) ;
  • Kim, Jeong-Soo (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Choi, Hong-Soo (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University)
  • Published : 2008.06.30

Abstract

A peculiar virus-like disease of tomato showing yellow mosaic and necrotic spots on leaves and necrosis on veins, petioles and stems was observed at the Tomato Experimental Station (TES), Buyeo, Chungcheongnamdo, Korea. The disease incidence at TES fields ranged from 21 to 35% infecting different tomato cultivars. For this reason, to identify the virus infecting tomato and to characterize the virus based on biology, serology, cytology and at molecular level. Here, leaf samples were randomly collected from different infected tomato cultivars at TES fields and greenhouses and tested by ELISA using Pepper mottle virus (PePMoV) and Tomato mosaic virus (ToMV) antisera. Infected saps were mechanically inoculated in different host plants to test for pathogenicity, symptomatology and host ranges. Infected tissues and ultrathin sections were examined by electron microscopy. Finally, putative coat protein and 3'-untranslated region (CP/3'-UTR) fragment was amplified and cloned for sequence determination and analyzed its genetic relationship to existing PepMoV and PVY sequences at the Genbank. Results showed 69% of the samples were positive with PepMoV, 13% with ToMV and 19 % were doubly infected with PepMoV and ToMV. Symptoms greatly varied from different host plants inoculated with tomato leaf sap infected with PepMoV alone and discussed in detailed in this paper. Electron microscopy from infected tissues showed filamentous particles of 720-750nm in length, a typical morphology and size of PepMoV. In addition, cylindrical inclusion bodies, pinwheels, scrolls and laminates with masses of fibrillar inclusions were also found in ultrathin sections. Alignment of the sequences of the CP/3'-UTR revealed >96% sequence identity with PepMoV and only <61% with PVY. Taken together, all these evidences presented clearly indicated that the causal agent infecting tomato at TES was PepMoV and we designated this PepMoV infecting tomato as Tom-sd2 strain in this study.

Keywords

References

  1. Benner, C. P., Kuhn, C. W., Demski, J. W., Dobson, J. W., Colditz, P. and Nutter, F. W. 1985. Identification and incidence of pepper viruses in northeastern Georgia. Plant Dis. 69:999-1001.
  2. Boonham, N., Hims, M., Barker, I. and Spence, N. 1999. Potato virus Y from petunia can cause symptoms of potato tuber necrotic ringspot disease (PTNRSD). Eur. J. Plant Pathol. 105:617-621. https://doi.org/10.1023/A:1008715224185
  3. Brunt, A. A., Crabtree, K., Dallwitz, M. J., Gibbs, A. J., Watson, L. and Zurcher, E. J. 1996. Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 2 0th August 1996. 'URL http://biology.anu.edu.au/Groups/MES/vide/'.
  4. Carr, R. J. and Kim, K. S. 1983. Evidence that bean golden mosaic virus invades non-phloem tissue in double infections with tobacco mosaic virus. J. Gen. Virol. 64:2489-2492. https://doi.org/10.1099/0022-1317-64-11-2489
  5. Choi, H. S., Cho, J. D. and Kim, J. S. 1997. Tomato infecting viruses and disease occurrence in Korea. Korean J. Plant Pathol. 13:64-66.
  6. Choi, H.S., Kim, J. S., Lee, K. H. and Shim, J. S. 1993. Ultrastructure and virus replication in cells of rice plant infected with RBSDV. RDA J. Agric. Sci. 35:344-353.
  7. Choi, H. S., Ryu, J. K., Ahn, K. K., Cho, J. D. and Kim, J. S., 2001. Cucumber mosaic cucumovirus-CARNA5 causing bud necrosis on table tomato. Korean J. Plant Pathol. 17:169-173.
  8. Clark, M. F. and Bar-Joseph, M. 1984. Enzyme immunosorbent assays in plant virology. Methods Virol. 7:51-85. https://doi.org/10.1016/B978-0-12-470207-3.50009-7
  9. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.2307/2408678
  10. Frankel, M. J., Ward, C. W. and Shukla, D. D. 1989. The use of 3'-noncoding nucleotide sequences in the taxonomy of potyviruses: application to watermelon mosaic virus-2 and soybean mosaic. J. Gen. Virol. 70:2775-2783. https://doi.org/10.1099/0022-1317-70-10-2775
  11. Gebre-Selassie, K., Marchoux, G., Delecolle, B. and Pochard, E. 1985. Variabilité naturelle des souches du virus Y de la pomme de terre dans les cultures de piment du sud-est de la France. Agronomie 5:621-630. https://doi.org/10.1051/agro:19850708
  12. Han, J. H., Choi, H. S., Kim, D. H., Lee, H. R. and Kim, B. 2006. Biological, physical and cytological properties of Pepper mottle virus-SNU1 and its RT-PCR detection. Korean J. Plant Pathol. 22:155-160. https://doi.org/10.5423/PPJ.2006.22.2.155
  13. Kim, J. S., Yoon, M. K., Choi, H. S., Lee, K. H., Choi, G. S., Kim, J. Y. and Cho, J. D. 1995. Zucchini yellow mosaic virus from Cucurbita moschata in Korea. RDA J. Agric. Sci. 37:352-362.
  14. Ko, S. J., Lee, Y. H., Cha, K. H., An, U. Y. and Choi, H. S. 2003. Survey and identification of virus diseases on paprika in Jeonnam province. Korean J. Plant Pathol. 9:342.
  15. Lee, S. H. 1981. Studies on virus disease occurring in various crops in Korea. Res. Report RDA 23:62-74.
  16. Murphy, J. F. and Kyle, M. M. 1995. Alleviation of restricted systemic spread of pepper mottle potyvirus in Capsicum annuum cv. Avelar by coinfection with a cucumovirus. Phytopathology 85:561-566. https://doi.org/10.1094/Phyto-85-561
  17. Nelson, M. R. and Wheeler, R. E. 1978. Biological and serological characterization and separation of potyviruses that infect peppers. Phytopathology 68:979-984. https://doi.org/10.1094/Phyto-68-979
  18. Nelson, M. R., Wheeler, R. E. and Zitter, T. A. 1982. CMI/AAB Description of Plant Viruses No. 253.
  19. Nelson, M. R. and Wheeler, R. E. 1972. A new virus disease of pepper in Arizona. Plant Dis. Reptr. 56:731-735.
  20. Oruetxebarria, I., Kekarainen, T., Spetz, C. and Valkonen, J. P. T. 2000. Molecular characterization of Potato virus V genomes from Europe indicates limited spatiotemporal strain differentiation. Phytopathology 90:437-444. https://doi.org/10.1094/PHYTO.2000.90.4.437
  21. Pappu, S. S., Brand, A., Pappu, H. R., Rybicki, E. P., Gough, K. H. and Frankel, M. J., Niblett, C. L. 1993. A polymerase chain reaction method adopted for selective amplification and cloning of 3'-sequences of potyviral genomes: application to dasheen mosaic virus. J. Virol. Methods 41:9-20. https://doi.org/10.1016/0166-0934(93)90158-N
  22. Prescott, A. and Martin, C. 1987. A rapid method for the quantitive assessment of levels of specific mRNAs in plants. Plant Molec. Biol. Reptr. 4:219-224.
  23. Purcifull, D. E., Zitter, T. A. and Hiebert, E. 1975. Morphology, host range and serological relationships of pepper mottle virus. Phytopathology 65:559-562. https://doi.org/10.1094/Phyto-65-559
  24. Revers, F., Le Gall, O., Candresse, T., Le Romancer, M. and Dunez, J. 1996. Frequent occurrence of recombinant potyvirus isolates. J. Gen. Virol. 77:1953-1965. https://doi.org/10.1099/0022-1317-77-8-1953
  25. Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstruction of phylogenetic trees. Molec. Biol. Evol. 4:406-425.
  26. Sherwood, J. L., Reddick, B. B. and Conway, K. E. 1988. Reactions of Bahamian hot chile to single and double infections with tobacco mosaic virus and potato virus Y. Plant Dis. 72:14-16. https://doi.org/10.1094/PD-72-0014
  27. Shukla, D. D. and Ward, C. W. 1988. Amino acid sequence homology of coat protein as a basis for identification and classification of the potyvirus group. J. Gen. Virol. 69: 2703-2710. https://doi.org/10.1099/0022-1317-69-11-2703
  28. Shukla, D. D., Ward, C. W. and Brunt, A. A. 1994. The Potyviridae. CAB International, Cambridge, UK.
  29. Vance, V. B., Jordan, R., Edwardson, J. R., Christie, R., Purcifull, D. E., Turpen, T. and Falk, B. 1992. Evidence that pepper mottle virus and potato virusY are distinct viruses: analyses of the coat protein and 3' untranslated sequence of a California isolate of pepper mottle virus. Arch. Virol. [Suppl 5]:337-345.
  30. Vance, V. B., Moore, D., Turpen, T. H., Bracher, A. and Hollowell, V. C. 1992. The complete nucleotide sequence of pepper mottle virus genomic RNA: comparison of the encoded polyprotein with those of other sequenced potyviruses. Virology 191:19-30. https://doi.org/10.1016/0042-6822(92)90162-I
  31. Verhoeven, J. Th., Willemen, T. M. and Roenhorst, J. W. 2002. First report of Pepper mottle virusin tomato. Plant Dis. 86:186.
  32. Villalon, B. 1975. Virus disease of bell peppers in South Texas. Plant Dis. Reptr. 59:858-862.
  33. Yoon, J. Y., Choi, H. S., Ryu, H. Y., Harm, Y. I. and Choi, J. K. 1998. Color breaking syndrome of Matthiola incana caused by double infection of CMV and TuMV. Korean J. Plant Pathol. 14:220-222.
  34. Zitter, T. A. 1972. Naturally occurring pepper virus strains in South Florida. Plant Dis. Reptr. 56:586-590.
  35. Zitter, T. A. and Cook, A. A. 1973. Inheritance of tolerance to a pepper virus in Florida. Phytopathology 63:1211-1212. https://doi.org/10.1094/Phyto-63-1211

Cited by

  1. Morphological, physiological and pathological variations among isolates of Colletotrichum falcatum that cause red rot of sugarcane vol.8, pp.10, 2014, https://doi.org/10.5897/AJMR2012.2469
  2. Molecular characterization of Korean Pepper mottle virus isolates and its relationship to symptom variations vol.144, pp.1-2, 2009, https://doi.org/10.1016/j.virusres.2009.04.003