• Title/Summary/Keyword: Panax quinquefolium L

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Genetic Diversity Analysis of Wood-cultivated Ginseng using Simple Sequence Repeat Markers (SSR 마커를 이용한 산양삼의 유전적 다양성 분석)

  • Gil, Jinsu;Um, Yurry;Byun, Jae Kyung;Chung, Jong Wook;Lee, Yi;Chung, Chan Moon
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.6
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    • pp.389-396
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    • 2017
  • Background: Panax ginseng C. A. Meyer is wood-cultivated ginseng (WCG) in Korea which depends on an artificial forest growth method. To produce this type of ginseng, various P. ginseng cultivars can be used. To obtain a WCG similar to wild ginseng (WG), this method is usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. Recently, the WCG industry is suffering a problem in that Panax notoginseng (Burk.) F. H. Chen or Panax quinquefolium L. are being sold as WCG Korean market; These morphological similarities have created confusion among customers. Methods and Results: WCG samples were collected from five areas in Korea. After polymerase chain reaction (PCR) amplification using the primer pair labeled with fluorescence dye (FAM, NED, PET, or VIC), fragment analysis were performed. PCR products were separated by capillary electrophoresis with an ABI 3730 DNA analyzer. From the results, WCG cultivated in Korea showed very diverse genetic background. Conclusions: In this study, we tried to develop a method to discriminate between WCG, P. notoginseng or P. quinquefolium using simple sequence repeat (SSR) markers. Furthermore, we analyzed the genetic diversity of WCG collected from five cultivation areas in Korea.

Ginseng Response to Graminicides (그래미니시드에 대한 인삼의 반응)

  • V.Souza Machado;Ali, A.
    • Journal of Ginseng Research
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    • v.13 no.1
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    • pp.14-18
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    • 1989
  • The graminicides fluazifop-p-butyl and sethoxydim effectively controlled annual grass weeds in 1,2 and year old ginseng crops during a 2 year study. Ginseng plants in the graminicide treated plots showed an early senescence of the shoots ; and at harvest the average root weight (g/root) and yield (kg/m) were slightly lower in these plants than in the untreated check plots. Roots of the graminicide treated plots remained viable to regenerate shoots. The greenhouse study indicated that shoot growth and seed development in ginseng plants were more sensitive to the graminicide treatment than the roots.

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Effects of Soil Moisture on the Growth of American Ginseng (Panax quinquefolium L.)

  • Li, Thomas S.C.;Berard, R.G.
    • Journal of Ginseng Research
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    • v.22 no.2
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    • pp.122-125
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    • 1998
  • Adequate available soil moisture level is considered to be one the most important components in growing high yields of good quality ginseng. Excessive soil moisture may promote stillborn fungal pathogens and cause serious diseases in ginseng fields. This study showed that soil moisture levels for optimum growth and health of ginseng varied with soil texture. Fifty- percent available moisture for sandy loam and 75% for silty loam are the best moisture levels for good growth and higher yield.

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Dynamic Studies on Physiology and Biochemistry in American Ginseng Seed During Stratification Part III. POD Activity, Contents of DNA and RNA, Isozymes of POD and ES - (충적과정중 미국인삼 종자의 생리 및 생화하적 지표에 관한 동태학적 연구 III)

  • Huang, Yao-Ge;Li, Xiang-Gao;Kuang, Ya-Lan;Yan, Jie-Kun;Cui, Shu-Yu;Yu, Wen-Bo;Yang, Ji-Xiang;Liu, Ren-Song;Kim, Hack-Seang
    • Journal of Ginseng Research
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    • v.21 no.1
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    • pp.43-48
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    • 1997
  • Dynamic parameters of biochemistry including the POD (peroxidase) activity, contents of DNA and RNA, isozymes of POD and ES (esterase) in American send (Panax quinquefolium L.) seed are reported in the present paper. The dynamic changes of POD activity proved that the PAS (physiological afterripening stage) is a stage in which some substances are prepared for seed germination. The POD activity correlated with ER (embryo ratio) significantly DNA content changed little only within 0.0036∼0.013 mg/ml, which did not correlate with ER. RNA content changed from 0.1539 to 1.0313 mg/ml and correlated significantly with RE during all of the embryo afterripening. None of the POD isozyme band was obtained in ESGS (embryo slowly growth stage), but five bands in ERGS (embryo rapidly growth stage) and six bands in PAS. Four bands of ES isozymes were obtained in ESGS, but six bands in ERGS, particularly, the content of ES isozymes increased in PAS. All of these may provide some information for understanding the dormancy mechanisms of American song seed.

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Photosynthesis Rate of American Ginseng under the different Monochromatic Light (미국인삼의 광합성에 미치는 단색광의 영향)

  • ;John T. A. Proctor
    • Journal of Ginseng Research
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    • v.12 no.1
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    • pp.87-91
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    • 1988
  • Photosynthesis rates of ginseng (Panax quinquefolium) leaves were measured in a controlled environment at $20^{\circ}C$ under the different monochromatic light such as white(W), red(R) and blue(B) to obtain basic information applicable to the colored shading material for the ginseng growth. Photosynthesis rate relative to white(W) light was generally higher in R and lower in B comparing to white(W) light. This difference was negligible at the close to the light-saturation point, whereas the difference among the monochromatic light was extended with decreasing the irradiant. It suggests that red is good in color of shading material for growth of American ginseng.

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Dynamic Studies on Physiology and Biochemistry in American Seng Seed During Stratification - Part I. Embryo Ratio, Dry Weight Ratio and respiration Rate (충적과정 중 미국삼 종자의 생리 및 생화학적 지표에 관한 동태학적 연구 I. 배아율, 건조중량비 및 호흡강도)

  • Huang, Yao-Ge;Li, Xiang-Gao;Yang, Ji-Xiang;Kuang, Ya-Lan;Yan, Jie-Kun;Cui, Shu-Yu;Liu, Ren-Song;Kim, Hack-Seang
    • Journal of Ginseng Research
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    • v.20 no.3
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    • pp.325-330
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    • 1996
  • Dynamic parameters of physiology including embryo ratio (ER), embryo length to endosperm length, dry weight ratio between embryo and endosperm (DWR) and respiration rate (RR) in American send (Panax quinquefolium L.) seed were investigated. According to the changes of ER during seed stratification, the duration of embryo afterripening could be divided into three stages as \circled1 embryo slow growth stage (ESGS), ER increased from 7.31% to 20.48% (0.16% day-1): \circled2 embryo rapid growth stage (ERGS), ER increased to 80.98% (0.61% day-1) (75G5+ ERGS=morphological afterripening stage (MP,5)) and \circled3pysiological afterripening stage (PAS), ER Increased to 88.50% (0.094 day-1) only. DWR Increased slowly from 0.20% to 2.76clp (0.016% day-1) in MAS and rapidly to 8.81% (0.061% day-1) in PAS. The RR correlated significantly with ER as well (r=0.8934 > rot, 0.6610). The steep increment of both DWR and RR in PAS indicated that the PAS was not a static stage although the ER was not changed too much. All of these may provide some information for understanding the dormancy mechanisms of American sting seed.

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Effect of Hot Water Boiling and Autoclaving on Physicochemical Properties of American Ginseng (Panax quinquefolium L.)

  • Kim, Kyung-Tack;Yoo, Kyung-Mi
    • Journal of Ginseng Research
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    • v.33 no.1
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    • pp.40-47
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    • 2009
  • This study evaluates changes in the chemical composition and bioactivities of American ginseng (Panax quinquefolius L.) processed by boiling in water, $75^{\circ}C$ for 10, 20, 30, and 40 min, and autoclaving at high temperatures, $115^{\circ}C$ for 30 and 60 min and $130^{\circ}C$ for 90 and 120 min. Total ginsenoside contents of boiled ginseng remained relatively unchanged, whereas the contents of autoclaved ginseng samples significantly decreased with an increase of both time and temperature. Compared to unheated ginseng (control), the color of both boiled and autoclaved ginseng decreased in lightness and increased in redness. The acidic polysaccharide contents, the total phenolic contents and the antioxidant capacity of boiled and autoclaved ginseng were higher than the untreated ginseng, with the highest values being exhibited by the autoclaved samples. In particular, the antioxidant capacity of unheated ginseng increased about 2.5 times ($285.7{\pm}14.03\;mg$/100g to $777.2{\pm}26.4\;mg$/100g) when ginseng was autoclaved at $130^{\circ}C$ for 120 min as compared to the control. It was concluded that as American ginseng was processed at a high temperature, especially steam-heated in an autoclave, its chemical constituents changed and, in particular, acidic polysaccharides, total phenolics and antioxidant capacity were considerably increased.

Dynamic Studies on Physiology and Biochemistry in American Ginseng Seed During Stratification - Part II. Contents of Soluble Carbohydrate, Crude Fat, Fatty Acid and Soluble Protein - (충적과정중 미국인삼 종자의 생리 및 생화학적 지표에 관한 동태학적 연구 I)

  • Huang, Yao-Ge;Li, Xiang-Gao;Cui, Shu-Yu;Yu, Wen-Bo;Kuang, Ya-Lan;Yan, Jie-kun;Yang, Ji-Xiang;Liu, Ren-Song;Kim, Hack-Seang
    • Journal of Ginseng Research
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    • v.21 no.1
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    • pp.39-42
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    • 1997
  • Dynamic parameters of biochemistry including the contents of soluble carbohydrate (SC) , crude fat (CF) , fatty arid (FA) and soluble protein (SP) in American send (Panax qufnquelolium L.) seed are reported in the present paper. When embryo ratio (ER) increased from 7.31 to 20.48%, the SC content decreased from 4.08 to 1.16%. After that, SC content changed little. The CF content decreased successively from 34.50 to 14.40% from the beginning to the end during the embryo afterripening. The changes of FA content did not correlate with that of ER and the changing range of SP content was not large during the seed stratification. All of these may provide some information for understanding the dormancy mechanisms of American seng seed.

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Comparison of Seed Oil Characteristics from Korean Ginseng, Chinese Ginseng (Panax ginseng C.A. Meyer) and American Ginseng (Panax quinquefolium L.)

  • Zhu, Xue-Mei;Hu, Jiang-Ning;Shin, Jung-Ah;Lee, Jeung-Hee;Hong, Soon-Teak;Lee, Ki-Teak
    • Preventive Nutrition and Food Science
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    • v.15 no.4
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    • pp.275-281
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    • 2010
  • The chemical characteristics of seed oils of Asian ginseng (Panax ginseng C.A. Meyer) at different ages grown in Korea (3, 4 and 5-year old) and China (5-year old), and American ginseng (Panax quinquefoliu L., 5-year old) grown in China were compared. Total fatty acid composition showed a significantly higher oleic acid content in American (87.50%) than in Korean (68.02~69.14%) and Chinese ginseng seed oils (61.19%). At the sn-2 position, the highest oleic acid (81.09%) and lowest linoleic acid (15.77%) were found in American ginseng seed oil. The main triacylglycerol species in ginseng seed oils were triolein (OOO) and 1,2-dioleoyl-3-linoleoyl-glycerol (LOO)/1,3-dioleoyl-2-linoleoyl-glycerol (OLO). In addition, the seed oils possessed an ideal oxidative stability showing 16.55~23.12 hr of induction time by Rancimat test. The results revealed that ginseng seed oil could be developed as a new healthy edible oil, and that the oil's chemical characteristics were strongly associated with the ginseng species and habitats.

Comparison of Quality on the Raw and Red Ginseng in Korean and American Ginseng (고려인삼과 미국삼의 수삼 및 홍삼품질 비교)

  • Chung, Chan-Moon;Shin, Ju-Sik
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.3
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    • pp.183-187
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    • 2006
  • This study carried out to analize quality in the evaluation based on the grades such as raw ginseng and red ginseng between Korean (Panax ginseng C. A. Meyer) and American ginseng (Panax quinquefolium L). American ginseng was small in root length, root diameter and root weight and emergence many secondary root from rhizome and main root. Therefore body form was bad compared with Korean ginseng. As for yield of dry, Korean ginseng was 30.4 % and American ginseng was 33.8% but as for yield, Korean ginseng was 80.4% and American ginseng was 72.2%. There were as many Korean ginseng in raw ginseng first grade by about twice compared with American ginseng. However there were many American ginseng in second grade. On the other hand, Korean ginseng producted high Chunsam compared with American ginseng. The cause of decreasing quality was inside cavity and inside white in Korean and American ginseng. Especially, the inside white occurrence about 3 times compared with inside cavity in American ginseng. In the case of distribution of weight size, Korean ginseng was much above 20Ji but American ginseng was much under 20Ji.