• Korean Journal of Medicinal Crop Science
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• v.26 no.3
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• pp.240-247
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• 2018

Background: Saline soil has negative effects on the growth of most crops. Sodium is the main element that causes salt accumulation in soil. Organic materials such as cow and poultry manure, are frequently used during the preparation stage, which causes an increase in the rate of salt accumulation in the soil. Methods and Results: To investigate the influences of sodium on ginseng, $NaH_2PO_4$, $Na_2SO_4$, and NaCl were used to adjust the sodium concentrations at 0, 12.5, 25, 50, 75 and 100 mM in nutrient solution. In a 2-year-old ginseng, toxic symptoms appeared when the sodium treatment exceeded 50 mM. The sodium concentration in the leaves was 3.33%, which is more than twice as high as that of the control treated at 50 mM. As the sodium concentration increased, the root weight significantly decreased. In the 100 mM treatment, the weight decreased by 28% when compared to that of the control. The Amount of ginsenoside significantly increased with an increase in sodium concentrations. Conclusions: These results suggest that the growth of 2-year-old ginseng is negatively affected when sodium exceeds 50 mM. This result can be used for a as basis in diagnosing the physiological disorders of ginseng.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.4
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• pp.504-508
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• 1983

To define the effects of synthetic auxins on rooting from the ginseng stern cutting and the root growth in diameter after the rooting of the cuttings, stern cuttings with palmate leaves obtained from seedlings and 2-year old ginseng plants were planted in rooting media treated with solutions of the synthetic auxins. All the roots induced from the cuttings were adventitious fibrous roots at first, but a few adventitious roots of the cutting were thickened in diameter to over 2 to 3mm at 120 days after cutting and the rest of them disappeared. IBA was the most effective auxin for rooting and root growth in diameter after rooting from the cuttings. The shape of the roots that thickened in diameter could be divided into two types. Both types of thickened roots were fully lignified at 120 days after cutting and those thickened roots did not dry up or die by July of the next year, however no rhyzomes or shoot primodia were induced from them.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.256-260
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• 2008

This study was conducted to response the photosynthetic rate and anatomical characters in one year ginseng plant against the light transmission ratio (10%, 20% and 30% in natural light) under shading materials by polyethylene (PE) net. The chlorophyll content and the photosynthesis of ginseng according to the light transmissivity showed decreasing trend as the light transmission ratio increased. At the higher light transmission ratio condition, leaves showed the chlorosis due to the physiological disorder, stems formed hypodermis, and phloem development inside the vascular tissue shrunk remarkably. Also, roots showed the epidermis destruction and less cambium development at the higher light intensity. The root weight also decreased as the light transmission ratio increased.

• Journal of Ginseng Research
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• v.34 no.3
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• pp.227-236
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• 2010

The objective of this study was to evaluate the potential use of ginseng leaf as a cosmetic material. In this research, we employed enzymatic treated ginseng leaf by using Ultraflo L to improve the recovery of ginsenosides from the ginseng leaf and studied the biological activities and skin safety of the enzymatic treated ginseng leaf for use as a cosmetic material. The total ginsenoside contents of the non-enzymatic treated ginseng leaf (NEGL) and Ultraflo L treated ginseng leaf (UTGL) were 271 and 406 mg/g, respectively. The level of metabolite ginsenosides (sum of Rg2, Rg3, Rg5, Rk1, compound K, Rh1, Rh2, and F2) was higher in UTGL (93.1 mg) compared to NEGL (62.4 mg) in one gram ginseng leaf extract. The increase in amounts of ginsenoside types in UTGL compared to NEGL was generally 140% to 157%. UTGL exhibited relatively higher 2,2-diphenyl-2-picrylhydrazyl hydrate ($IC_{50}$, 2.8 mg/mL) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ($IC_{50}$, 1.6 mg/mL) radical scavenging activities compared to NEGL (4.8 mg/mL and 2.2 mg/mL). The UTGL group showed normalized hydrogen peroxide, lipid peroxidation and visual wrinkling grade induced-UVB exposure. The UTGL did not induce any adverse reactions such as erythema and edema on intact skin sites; however, some guinea pigs treated with UTGL on abraded skin sites showed very slight erythema. The primary irritation index (PII) score of UTGL was 0.05 and it was classified as a practically non-irritating material (PII, 0 to 0.5). In skin sensitization tests with guinea pigs, UTGL had a positive rate of skin sensitization at 40%, and the mean evaluation score was 0.4.

• Journal of Ginseng Research
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• v.44 no.5
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• pp.747-755
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• 2020

Background: Ginsenosides accumulation responses to temperature are critical to quality formation in cold-dependent American ginseng. However, the studies on cold requirement mechanism relevant to ginsenosides have been limited in this species. Methods: Two experiments were carried out: one was a multivariate linear regression analysis between the ginsenosides accumulation and the environmental conditions of American ginseng from different sites of China and the other was a synchronous determination of ginsenosides accumulation, overall DNA methylation, and relative gene expression in different tissues during different developmental stages of American ginseng after experiencing different cold exposure duration treatments. Results: Results showed that the variation of the contents as well as the yields of total and individual ginsenosides Rg1, Re, and Rb1 in the roots were closely associated with environmental temperature conditions which implied that the cold environment plays a decisive role in the ginsenoside accumulation of American ginseng. Further results showed that there is a cyclically reversible dynamism between methylation and demethylation of DNA in the perennial American ginseng in response to temperature seasonality. And sufficient cold exposure duration in winter caused sufficient DNA demethylation in tender leaves in early spring and then accompanied the high expression of flowering gene PqFT in flowering stages and ginsenosides biosynthesis gene PqDDS in green berry stages successively, and finally, maximum ginsenosides accumulation occurred in the roots of American ginseng. Conclusion: We, therefore, hypothesized that cold-induced DNA methylation changes might regulate relative gene expression involving both plant development and plant secondary metabolites in such cold-dependent perennial plant species.

• Korean Journal of Medicinal Crop Science
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• v.26 no.6
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• pp.464-470
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• 2018

Background: Ginseng produced by hydroponics can be cultivated without using agricultural chemicals; thus, it can be used as a raw materials for functional foods, medicines, and cosmetics. This study aimed to determine the optimal harvesting time to obtain the highest levels of ginsenoside and ginseng, as this was not previously unknown. Methods and Results: One-year-old organic ginseng seedlings were transplanted and cultivated using hydroponics for 150 days in a venlo-type greenhouse, using ginseng nursery bed soil and a nutrient solution ($NO_3{^-}-N$; 6.165, P; 3.525, K; 5.625, Ca; 4.365, Mg; 5.085, S; $5.31mEq/{\ell}$). Ginsenoside content and fresh and dry weights were higher at 120 days after transplanting than at 30, 60, 90, and 150 days. Total ginsenoside content was 11.86 times higher in the leaf and stem than in the root at 120 days after transplanting. Ginsenosides F1, F2, F3, and F5 were detected in ginseng leaves and stems. These chemical compounds are known to be effective in altering skin properties, including whitening, anti-inflammation, and anti-aging. Conclusions: Optimal harvesting time for ginseng cultivated using hydroponics was 120 days after transplanting when the biomass and ginsenoside content were highest.

• Journal of Ginseng Research
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• v.43 no.4
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• pp.645-653
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• 2019

Background: Cytochrome P450 enzymes catalyze a wide range of reactions in plant metabolism. Besides their physiological functions on primary and secondary metabolites, P450s are also involved in herbicide detoxification via hydroxylation or dealkylation. Ginseng as a perennial plant offers more sustainable solutions to herbicide resistance. Methods: Tissue-specific gene expression and differentially modulated transcripts were monitored by quantitative real-time polymerase chain reaction. As a tool to evaluate the function of PgCYP736A12, the 35S promoter was used to overexpress the gene in Arabidopsis. Protein localization was visualized using confocal microscopy by tagging the fluorescent protein. Tolerance to herbicides was analyzed by growing seeds and seedlings on Murashige and Skoog medium containing chlorotoluron. Results: The expression of PgCYP736A12 was three-fold more in leaves compared with other tissues from two-year-old ginseng plants. Transcript levels were similarly upregulated by treatment with abscisic acid, hydrogen peroxide, and NaCl, the highest being with salicylic acid. Jasmonic acid treatment did not alter the mRNA levels of PgCYP736A12. Transgenic lines displayed slightly reduced plant height and were able to tolerate the herbicide chlorotoluron. Reduced stem elongation might be correlated with increased expression of genes involved in bioconversion of gibberellin to inactive forms. PgCYP736A12 protein localized to the cytoplasm and nucleus. Conclusion: PgCYP736A12 does not respond to the well-known secondary metabolite elicitor jasmonic acid, which suggests that it may not function in ginsenoside biosynthesis. Heterologous overexpression of PgCYP736A12 reveals that this gene is actually involved in herbicide metabolism.

• Journal of Ginseng Research
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• v.45 no.1
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• pp.86-97
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• 2021

Background: Panax ginseng Meyer has been used as a nourishing edible herb in East Asia for thousands of years. 25-OH-PPT was first discovered as a natural rare triterpenoid saponin in ginseng stems and leaves by our group. Research found that it showed strong inhibitory effects on α-glucosidase and protein tyrosine phosphatase 1B, and protected cardiocytes (H9c2) through PI3K/Akt pathway. Methods: In the research, in order to optimize the 25-OH-PPT enrichment process, optimal macroporous resins and optimal purification conditions were studied. Meanwhile, the hypoglycemic effect and mechanism of 25-OH-PPT were evaluated by using STZ to establish insulin-dependent diabetic mice and the spontaneous type 2 diabetes DB/DB mice. Results and Conclusion: Research found that 25-OH-PPT can reduce blood glucose and enhance glucose tolerance in STZ model mice. It increases insulin sensitivity by upregulating GLUT4 and AMPK in skeletal muscle, and activating insulin signaling pathways. In DB/DB mice, 25-OH-PPT achieves hypoglycemic effects mainly by activating the insulin signaling pathway. Meanwhile, through the influence of liver inflammatory factors and lipids in serum, it can be seen that 25-OH-PPT has obvious anti-inflammatory and lipid-lowering effects. These results provide new insights into the study of ginseng as a functional food.

• Korean Journal of Soil Science and Fertilizer
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• v.12 no.1
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• pp.49-53
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• 1979

Effects of temperature and light intensity on photosynthesis, respiration and chlorophyll content of ginseng (Panax ginseng C. A. Meyer) were as follow. 1. Optimum light intensity for apparent photosynthesis at $25^{\circ}C$ was 25Klux($1.35mgCO_2/dm^2{\cdot}hr$) for two years old ginseng grown in pot and 30Klux($1.94mgCO_2/dm^2{\cdot}hr$) for the six years old palmate cut leaves. Optimum temperature at 25Klux was $15^{\circ}C$ ($1.40mgCO_2$) for the 2 years old and 20 to $22^{\circ}C$ ($2.03mgCO_2$) for the 6 years old. 2. Dark respiration increased almost linearly with the increase of air temperature till $25^{\circ}C$ (2.6times between $16^{\circ}C$ to $25^{\circ}C$ for the 6 years old and 1.8 times between $15^{\circ}C$ to $25^{\circ}C$ for the 2 years old). Dark respiration was 11.1 % of net photosynthesis at $16^{\circ}C$, 17.8% at $25^{\circ}C$ for the 6 years old and 40% at $15^{\circ}C$, 64.7% at $25^{\circ}C$ for 2 years old. 3. Stomata appeared only in abaxial surface (lower epidermis) and stomatal frequency was $37per\;mm^2$. 4. Above results together with other informations quoted here strongly suggest that air temperature is much better criteria than light intensity for the improvement of shading roof material and shading construction. That is to promise maximum light intensity unless air temperature is above $25^{\circ}C$.

• Korean Journal of Medicinal Crop Science
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• v.15 no.6
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• pp.367-370
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• 2007

To make the soil moisture proper is the important factor in the seedbed cultivation of Yangjik for producing a good quality of ginseng seedling. This study was carries out to investigate the effect of soil moisture on photosynthesis and yield of ginseng seedling under the different condition of the soil moisture, such as $100{\sim}400$ mbar. Photosynthesis rate was decreased gradually by the reduction of soil moisture, and in particular it was decreased distinctly under the lower condition of soil moisture, such as $300{\sim}400$ mbar. Photosynthesis rate in air temperature of $30^{\circ}C$ was decreased more distinct than that of $25^{\circ}C$, Light saturation point of leaves was at the quantum of $600{\mu}mol/m^3/s$ at $25^{\circ}C$ while it was decreased by $300{\mu}mol/m^3/s$ at $30^{\circ}C$ according to the increase of air temperature. Respiration rate was increased by the increase of quantum, and decreased by the reduction of soil moisture. Respiration rate under the condition of high quantum was increased regardless of air temperature, but it was decreased distinctly under the condition of low soil moisture and high air temperature, such as 400 mbar at $30^{\circ}C$. There were a gradual decrease by the reduction of soil moisture in leaf length, leaf width, chlorophyll content, and water content of leaves, but heat injury ratio was increased distinctly by the reduction of it. Total root weight, root weight per plant, the yield of usable seedling were decreased by the reduction of soil moisture, and optimal content of soil moisture to produce a good quality of seedling was 63% of field capacity or 18.9% in absolute soil moisture content.