• 한국약용작물학회지
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• 제17권5호
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• pp.352-356
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• 2009

In this study, raw ginseng water extract solutions were analyzed to set up the functional saponin content and quality optimization condition. The highest saponin content among the total raw ginseng water extracts was $74.6\;mg/100\;m{\ell}$ which was extracted at $75^{\circ}C$ for 24 hours. In addition, the saponin content decreased according to the increased extraction temperature and time. The highest total content of $Rb_2$ and Re was $19.9\;mg/100\;m{\ell}$ at $75^{\circ}C$ for 12 hours which decreased according to the increased extracted temperature and time. The highest prosapogenin ($Rg_2\;+\;Rg_3\;+\;Rh_1$) content among the total raw ginseng water extracts was $28.6\;mg/100\;m{\ell}$ which was extracted at $85^{\circ}C$ for 36 hours. The reducing sugar content, sweetness and turbidity were increased according to the increased extraction temperature and time. But pH were decreased according to the increased extracted time.

• Journal of Applied Biological Chemistry
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• 제50권2호
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• pp.78-84
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• 2007

The anti-fibrotic effects of hot water extract of adventitious root culture of Panax ginseng (ARCP) and the possible mechanisms were investigated on $CCl_4-induced$ hepatotoxicity model mice. Fibrosis was induced by a mild treatment of $CCl_4$. Then silymarin as a positive control drug and ARCP or carrier alone as a negative control were treated. Serum GPT, GOT and ALP activity levels were lowered by 25, 21 and 11% for silymarin treated group and by 48, 39 and 14% for ARCP treated group compared to carrier treated alone. Hepatic collagen for ARCP treatment group was reduced by 18% and MDA contents decreased a little more. Pro-fibrotic gene ($TGF-{\beta}1$, TIMP-1 and ${\alpha}-SMA$) expression increased following the $CCl_4$ treatment, but both the silymarin and the ARCP treatments decreased the expressions of these genes by 20% to 50%. The antioxidant effect of ARCP was studied by DPPH free radical scavenging activity. In addition, a generation of reactive oxygen species (ROS) was also reduced in $H_2O_2-treated$ HepG2 cells upon the ARCP treatment. In summary, ARCP has antioxidant property, and can have some protection against oxidative stress; more importantly, ARCP can efficiently protect mice against $CCl_4-induced$ fibrosis.

• 한국약용작물학회지
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• 제23권2호
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• pp.117-124
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• 2015

Osteoporosis induces a bone mineral density loss due to imbalance of bone homeostasis that is achieved by osteoclasts (which are involved in bone resorption) and osteoblasts (which are involved in bone formation). Thus, this study was performed to evaluate the effects of hot water extract of the Achyranthes bidentata Blume (ABB) and Panax ginseng (Gin) on osteoclast and osteoblast differentiation. In this study, there was no cytotoxicity by ABB, 50 and $100{\mu}g/ml$ of Gin significantly decreased cell viability of RANKL-induced osteoclast in RAW264.7 cell (p < 0.01). But, it was $50{\mu}g/ml$ of ABB and Gin mixtures increased due to protective action of ABB. Furthermore, Gin contained groups (Gin, ABB and Gin mixtures) were inhibitory effects on osteoclast differentiation and bone resorption, and increased in osteoblast differentiation activity. Gin clearly inhibited RANKL-induced osteoclast differentiation by decreased calcitonin and TRAP (p < 0.01). Also, these extracts significantly increased calcium accumulation formation of osteoblastic differentiation reagents-induced osteoblast in MC3T3-E1 cell (p < 0.05). These results suggest that ABB and Gin mixtures may be a potential as drug for the treatment of osteoporosis.

• Journal of Ginseng Research
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• 제19권3호
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• pp.225-230
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• 1995

The effect of long-term ginseng (Panax ginseng C.A. Meyer) administration on the clearance of carboxyhemoglobin (CO-Hb) and the property of blood gases was investigated in rats. Rats were received ginseng water extract (0.025% in drinking water) for 42 weeks starting at the age of 6 weeks. They were exposed to the diluted mainstream smoke generated from 15 filter cigarettes for 20 min in a round polycarbonate chamber (D37 cmXH13 cm). Under this condition, the mean CO-Hb content of control and the ginseng-treated rats immediately after the exposure was nearly the same as 13.8$\pm$2.9 f) and 13.9$\pm$1.6%, respectively. However, CO-Hb was more rapidly removed from blood in the ginseng treated rats than in untreatEd control with the laps of time, namely, its biological half life In the former was 36.9$\pm$1.5 min and in the latter was 56.9$\pm$13.2 min. Although long-term ginseng treatment did not affect the content of hemoglobin and blood pH of rats, it slightly increased blood oxygen content and its partial pressure value, and decreased levels of carbon dioxide and bicarbonate. These results suggest that long-term administration of rats with ginseng extract accelerate the elimination of CO from the blood. This effect seems to be related to the enhancement of oxygen consumption of the rat by a certain action of ginseng components as previously reported.

• 한국유기농업학회지
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• 제22권4호
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• pp.705-718
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• 2014

본 연구는 인삼점무늬병과 탄저병에 대한 친환경자재로 만들어진 친환경 살균제의 억제효과를 알아보기 위해서 수행을 했다. 인삼 점무늬병과 인삼 탄저병들은 인삼뿌리가 비대하기 시작하기 전에 낙엽을 시켜서 수확량을 크게 감소하는 주요한 병들이다. 천연칼슘, 녹차추출물, 홍삼박 술등은 A. panax에 의해서 유발된 인삼 점무늬병에 대해서 상당한 억제효과를 보였고 게 껍질 발효액과 익모초자연즙액은 C. gloeosporioides에 의해서 유발된 인삼탄저병에 대해서 큰 억제효과를 나타내었다. 이들 친환경 살균제는 치료효능보다 대체적으로 예방효능이 더 우수 하였다. 그러므로 이들 친환경 자재들은 인삼 유기재배포장에서 사용할 수 없는 화학농약을 대체하는 살균제로써 이용할 수 있고 그들의 억제효과를 극대화하기 위해 발병 전에 처리를 해야 한다.

• Journal of Ginseng Research
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• 제38권1호
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• pp.34-39
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• 2014

Cigarette smoke is considered a major risk factor for vascular diseases. There are many toxic compounds in cigarette smoke, including acrolein and other ${\alpha},{\beta}$-unsaturated aldehydes, which are regarded as mediators of inflammation and vascular dysfunction. Furthermore, recent studies have revealed that acrolein, an ${\alpha},{\beta}$-unsaturated aldehyde in cigarette smoke, induces inflammatory mediator expression, which is known to be related to vascular diseases. In this study, we investigated whether Korean Red Ginseng (KRG) water extract suppressed acrolein-induced cyclooxygenase (COX)-2 expression in human umbilical vein endothelial cells (HUVECs). Acrolein-induced COX-2 expression was accompanied by increased levels of phosphorylated p38 in HUVECs and KRG inhibited COX-2 expression in HUVECs. These results suggest that KRG suppresses acrolein-induced COX-2 expression via inhibition of the p38 mitogen-activated protein kinase signaling pathway. In addition, KRG exhibited an inhibitory effect on acrolein-induced apoptosis, as demonstrated by annexin Vepropidium iodide staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay. Consistent with these results, KRG may exert a vasculoprotective effect through inhibition of COX-2 expression in acrolein-stimulated human endothelial cells.

• 한국식품위생안전성학회지
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• 제35권4호
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• pp.382-390
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• 2020

S. aureus, E. coli, C. albicans, A. niger 등 4종의 식중독균에 대해 agar diffusion법을 이용하여 홍삼(Panax ginseng C.A.Meyer)으로 부터 조제한 홍삼농축액, 조사포닌, 비수용성 분획에 대한 항균활성을 조사하였다. 그 결과, 홍삼농축액 및 비수용성분획은 E. coli, C. albicans, A. niger에 대해서는 항균활성을 나타내지 않았고 조사포닌도 고농도인 30%를 제외한 모든 농도에서 항균활성을 나타내지 않았다. S. aureus는 Gram positive 세균으로서 화농성 식중독의 원인균이면서 동시에 아토피성 피부염의 원인균으로 알려져 있는데, 홍삼농축액은 30% 농도에서 이 균에 대해 항균활성을 나타내었고 조사포닌도 7.5%에서 항균활성을 나타내었다. 홍삼으로부터 조제한 비수용성 분획도 10~200 mg/mL 농도로 실험한 결과 모든 분획에서 항균효과를 나타내었다. 조사포닌 및 홍삼농축액의 미생물 생육저해양상을 조사하기 위해 미리 S. aureus를 접종한 0.85% 생리식염수에 농축액 및 조사포닌을 농도별로 첨가하고 35℃, 12시간 배양한 후 생균수를 측정한 결과, 홍삼농축액은 10% 이상의 농도에서, 조사포닌은 2% 이상의 농도에서 각각 균의 생육을 억제하였다. 그러나 이러한 농도에서도 생균수는 완전히 사멸되지 않아서 홍삼농축액 및 조사포닌의 S. aureus에 대한 생육억제작용은 살균작용이 아닌 정균 작용으로 추정되었다. 사포닌의 항균활성 유무를 확인하기 위해 순수 분리된 ginsenoside 6종(PT saponin, PD saponin, ginsenoside-Rb₂,-Rc,-Rd,-Rf,-Rg₂)의 항균활성을 50~200 ㎍/mL의 농도에서 조사한 결과 모두 항균효과가 관찰되지 않아서 ginsenoside는 S. aureus에 대해서는 항균효과가 없는 것으로 사료된다. 한편 사포닌을 제외한 비사포닌 성분인 비수용성분획에 대해 상기의 4종 병원성미생물을 대상으로 항균활성을 조사한 결과 E. coli, C. albicans, A. niger에 대해서는 항균효과가 관찰되지 않았고 S. aureus에 대해서만 선택적인 항균활성을 나타내었다. 항균활성 발현 비수용성분획 중 15% methanol분획(MF-1)이 가장 높은 항균활성을 나타내어 이에 대한 최소생육저해농도를 조사한 결과 0.625 mg/mL 이었다. MF-1 분획을 질량분석기(HPLC-MS)로 조사한 결과 주요한 활성성분은 분자량 179.55 및 187.55를 가지는 물질로 추정되었다.

• Journal of Ginseng Research
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• 제39권4호
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• pp.384-391
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• 2015

It has been reported that Korean Red Ginseng has been manufactured for 1,123 y as described in the GoRyeoDoGyeong record. The Korean Red Ginseng manufactured by the traditional preparation method has its own chemical component characteristics. The ginsenoside content of the red ginseng is shown as Rg1: 3.3 mg/g, Re: 2.0 mg/g, Rb1: 5.8 mg/g, Rc:1.7 mg/g, Rb2: 2.3 mg/g, and Rd: 0.4 mg/g, respectively. It is known that Korean ginseng generally consists of the main root and the lateral or fine roots at a ratio of about 75:25. Therefore, the red ginseng extract is prepared by using this same ratio of the main root and lateral or fine roots and processed by the historical traditional medicine prescription. The red ginseng extract is prepared through a water extraction ($90^{\circ}C$ for 14-16 h) and concentration process (until its final concentration is 70-73 Brix at $50-60^{\circ}C$). The ginsenoside contents of the red ginseng extract are shown as Rg1: 1.3 mg/g, Re: 1.3 mg/g, Rb1: 6.4 mg/g, Rc:2.5 mg/g, Rb2: 2.3 mg/g, and Rd: 0.9 mg/g, respectively. Arginine-fructose-glucose (AFG) is a specific amino-sugar that can be produced by chemical reaction of the process when the fresh ginseng is converted to red ginseng. The content of AFG is 1.0-1.5% in red ginseng. Acidic polysaccharide, which has been known as an immune activator, is at levels of 4.5-7.5% in red ginseng. Therefore, we recommended that the chemical profiles of Korean Red Ginseng made through the defined traditional method should be well preserved and it has had its own chemical characteristics since its traditional development.

• Journal of Ginseng Research
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• 제47권4호
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• pp.552-560
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• 2023

Background: Ginseng Radix (Panax ginseng Meyer, Araliaceae) has been used medicinally to treat the brain and nervous system problems worldwide. Recent studies have revealed physiological effects that could potentially benefit cognitive performance or mood. The present study aimed to investigate the antidepressant effects of Korean red ginseng water extract (KGE) and its active component in an unpredictable chronic mild stress (UCMS)-induced animal model and elucidate the underlying mechanisms. Methods: The antidepressant potential of the UCMS model was evaluated using the sucrose preference test and open field tests. The behavioral findings were further corroborated by the assessment of neurotransmitters and their metabolites from the prefrontal cortex and hippocampus of rats. Three doses of KGE (50, 100, and 200 mg/kg) were orally administered during the experiment. Furthermore, the mechanism underlying the antidepressant-like action of KGE was examined by measuring the levels of brain-derived neurotrophic factor (BDNF)/CREB, nuclear factor erythroid 2-related factor 2 (Nrf2), and Kelch-like ECH-associated protein 1 (Keap1) proteins in the prefrontal cortex of UCMS-exposed rats. Results: KGE treatment normalized UCMS-induced depression-related behaviors. Neurotransmitter studies conducted after completing behavioral experiments demonstrated that KGE caused a reduction in the ratio of serotonin and dopamine, indicating a decrease in serotonin and dopamine turnover. Moreover, the expression of BDNF, Nrf2, Keap1 and AKT were markedly increased by KGE in the prefrontal cortex of depressed rats. Conclusion: Our results provide evidence that KGE and its constituents exert antidepressant effects that mediate the dopaminergic and serotonergic systems and expression of BDNF protein in an animal model.

• Preventive Nutrition and Food Science
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• 제13권3호
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• pp.212-218
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• 2008

We extracted red ginseng with various alcohol concentrations and evaluated total carbohydrate, uronic acid, polyphenols compounds and ginsenoside contents, and yields of alcohol extract. The water extraction (0% alcohol extraction) showed a high level of total carbohydrate content. 10% and 20% alcohol extraction showed the highest uronic acid contents (7,978.8 and $7,872.7\;{\mu}g/mL$ of extract, respectively). The efficiency order of the red ginseng extract (RGE) preparations in liberating polyphenols was: $0{\sim}50%$ alcohol${\geq}\;60%$ alcohol> $70{\sim}90%$ alcohol. Solid contents in RGE were decreased with increased alcohol concentration; the same tendency as with the results of total carbohydrate content. Total ginsenoside contents in $20{\sim}50%$ alcohol extracts showed similar levels ($442,962.9{\sim}47,930.8\;{\mu}g/mL$ of extract). Water extraction showed the lowest ginsenoside content ($14,509.4\;{\mu}g/mL$ of extract). The ginsenoside contents at above 60% alcohol were decreased with increased alcohol concentration. Generally, ginsenoside (Rg2, Rg1, Rf, Re, Rd, Rb2, Rc and Rb1) contents were increased with increased alcohol concentrations. However, Rg3 content was decreased with increases in alcohol concentration.