• Journal of Ginseng Research
• /
• 제19권1호
• /
• pp.51-55
• /
• 1995

The DNA fragment containing ginseng ribulose-1,5-bisphosphate carboxytase/oxygenase large subunit(rbcL) gene was cloned from the ginseng chloroplast EcoRl library by colony lift hybridization with tobacco rbcL gene probe. From the screened clone, the DNA fragment containing ginseng rbcL gene was digested with several restriction enzyme and analyzed by Southern blot hybridization for the construction of restriction map. The ginseng rbcL gene fragment was subcloned in pBluescript II SK + vector and sequence analysis was performed. The nucleotide sequence of ginseng rbcL gene was compared with those of petunia, tobacco, alfalfa, rice and barley, which showed a homology of 93.1%, 95.2%, 90.5%, 85.5% and 84.3%, respectively.

• Journal of Ginseng Research
• /
• 제19권3호
• /
• pp.249-253
• /
• 1995

In the browning reaction of Korean ginseng, it appears that enzymatic and non-enzymatic browning reaction occurred in the initial stage of heating fresh ginseng at low temperature, and then non-enzymatic browning reaction followed in the drying period after heating. Activation energy of the browning reaction for red ginseng was about 9.0 kcal/mol. Browning reaction of red ginseng was accede- rated with an increase in steaming time, and a great extent of browning reaction occurred between 60-90 min of steaming at 10$0^{\circ}C$. Browning pigments of red ginseng were mostly water soluble subset.

• 한국응용곤충학회지
• /
• 제20권4호
• /
• pp.223-227
• /
• 1981

These experiments were conducted to investigate the nematicidal effects of the insecticides, Mocap(O-Ethyl-S, S-dipropyl phosphorodithioate), Carbofuran(2,3-Dihydro-2,2-dimethyl benzofuranyl ethyl carbamate), Terbufos (S-tert-buthylthio methyl O,O-diethyl phosphordithioate) and their mixtures (Mocap+carbofuran, Mocap+Terbufos, Carbofuran+Terbufos) on growth of 2year-old ginseng, Panax ginseng C.A. Meyer, and the control of root-knot nematodes. There was no evidence of plant injury from insecticide treatment of ginseng, although the rate of emergence of the treated ginseng was slightly inhibited. The insecticide treatments showed no of-flavor of ginseng plant. Terbufos and Mocap provided heifer confrol of the root-knot nematodes than carbofuran alone and their mixtures. Mixtures of the insecticides showed antagonisitic effect to the root-knot nematodes.

• Journal of Ginseng Research
• /
• 제8권2호
• /
• pp.105-113
• /
• 1984

This study was conducted to investigate chemical and physical characteristics of pectin in Korean ginseng plant. The results obtained are as follows: 1, The molecular weight of the pectin in ginseng plant was in the range of 1.1-2.4x 104 and athydrouronic acid content in the pectin was 97.98%. 2. The intrinsic viscosity of pectin as well as apparent visosity of the pectin in the roots were increased with cultural period. 3. The IR spectra of ginseng pectin showed the OH stretch, C-H bending vibration and the vibration of ester group carboxyl. 4. Sugars present in galacturonic acid, glucose, arbinose, xylose, galactose and rhamnose.

• Journal of Ginseng Research
• /
• 제19권3호
• /
• pp.231-236
• /
• 1995

The antioxidant activity and the pharmacologically active constituents such as nucleosides and phenolic substancs of Panax ginseng C.A. Meyer (white ginseng powder) treated with gamma irradiation (Rl group) and ethylene oxide fumigation (EO group) were compared with those of non-treated ginseng (control group). High performance liquid chromatograms of nucleosides in three groups were similar with each other. The bathochromic effect of phenolic substances at W absorption region tends to be higher for EO group than Rl group. This tendency was also observed in heat treatment of each group. However, the antioxidant activity may have some effects on the labile property of phenolic substances. In summary the gamma-irradiated ginseng was more stable than the ethylene oxide-treated ginseng, but both ginseng samples gave the similar antioxidant activity.

• Journal of Plant Biology
• /
• 제35권1호
• /
• pp.45-51
• /
• 1992

인삼(Panax ginseng C.A. Meyer)의 채종전 홍숙 종자로부터 채종 후 미개갑 종자까지 단계별 배발달에 따른 배유세포내 단백과립의 변화상을 확인하여 다음과 같은 결과를 얻었다. 홍숙 초기단계의 종자의 배유세포에는 구형의 스페로솜이 산재하였으며, 액포내에는 저장 단백질이 축적되어 단백과립을 형성하였다. 홍숙 말기단계의 종자의 배유세포내 세포질은 대부분 스페로솜과 단백과립으로 충만하였고, 세포소기관은 거의 관찰할 수 없었으며, 단백과립은 전자밀도가 높은 무정형의 함유물을 가지고 있는 것과 균일한 단백질 기질로만 이루어져 있는 것 등으로 크게 구분되었다. 채종 후 후숙 처리를 하지 않은 미개갑 종자에서, 배유세포내 단백과립은 구상체(globoid), 단백질 결정체 등을 함유하고 있었고, 구상체는 다양한 형태의 전자밀도가 높은 물질을 가지고 있었다. 제형층은 배와 배유조직 사이에 위치하였으며, 이와 인접하여 배유세포벽의 분해양상과 셀루로우즈 미세섬유상이 관찰되었다. 제형층은 lipid body와 분해된 배유세포의 잔유물로 이루어져 있었다. 제형층과 인접한 배유세포의 단백과립은 퇴행성 변화를 나타내었고, 이로 인해 단백질 기질은 전자밀도가 점진적으로 낮아지는 결과를 얻었다.

• 식품기술
• /
• 제18권2호
• /
• pp.3-13
• /
• 2005

인삼의 효능 및 유효성분에 관한 수많은 문헌 및 연구사례가 보고 되어 있음에도 불구하고 인삼의 주성분인 ginsenosides의 합성경로나 그와 관련된 유전인자에 대하여서는 거의 밝혀져 있지 않다. 인삼의 생리적 특성규명 및 ginsenosides의 생합성 경로를 밝히기 위한 한 방법으로써 프로테옴 분석이 시도되었다. 본지에서는 고려인삼 (Panax ginseng C.A. Meyer)을 대상으로 이루어진 최근의 프로테옴 분석 및 이차 대사산물의 생산과 연관된 유전인자를 찾기 위하여 이루어진 인삼의 기능유전체 분석을 소개하고자 한다.

• Journal of Ginseng Research
• /
• 제14권3호
• /
• pp.416-420
• /
• 1990

Ultrastructural and anatomical features of the leaf were studied in Panax ginseng C.A. Meyer(ginseng). The ginseng leaf poorly developed palisade tissue and the size of mesophyll cell was larger and the chloroplast density was lower than that of Glycine max (soyben). Ginseng chloroplast was filled with highly stacked grana and condensely-arrayed thylakoid, so the stroma space was hardly absorbed. However, ginseng mesophyll tissue and chloroplast array did not reduce light energy entering the mesophyll chloroplast, and the high LHCP/CP ratio of ginseng thylakoid resulted in the absorption of excess photon. It is reasonable to assume that 1O1-photogenearation by excess light energy partially resulted from the anatomical and ultrastructural characteristics of the ginseng leaf.

• Journal of Ginseng Research
• /
• 제13권2호
• /
• pp.153-157
• /
• 1989

청경종 특성을 알기위하여 지상부 제형질, 광합성과 호흡 그리고 자식세대의 경색을 조사하였다. 청경종은 엽경종, 황열종의 경, 엽, 광합성과 호흡에 뚜렷한 차이가 나타나지 않았지만 5년근시 화경장 형질이 타 변종보다 길었다. 광합성에 있어서는 청경종이 뚜렷한 차이는 없는 경향이며 비교적 황열종이 높았으나 $20^{\circ}C$ 이상에서는 모든 변종에서 현저히 떨어지는 경향이었다. 청경종내 자식세대 일부는 경색이 분리되는 경향이나 자식사대가 거듭할수록 녹색경(청경종) 출현이 높았다.

• Journal of Ginseng Research
• /
• 제14권2호
• /
• pp.135-141
• /
• 1990

In order to elucidate the mechanism of the leaf-burning disease of ginseng (Panax ginseng C.A. Meyer), the relationships between thylakoid membrane peroxidation and chlorophyll bleaching were investigated in comparison with the ones of soybean (Glycine max L). When I measured the rate of lipid peroxidation in the thylakoids of ginseng and soybean by irradiation of light(60 w.m-2), it was identified that, the remarkably lower rate of lipid peroxidation was found in the ginseng thylakoid than the case of soybean. When lipid peroxidation of ginseng thylakoid was induced in the dark, chlorophyll contents of thylakoid was not changed. The results suggest that lipid peroxidation does not affect the chlorophyll bleaching in ginseng thylakoid. Thylakoid membrane peroxidation as well as chlorophyll bleaching was closely related with photosynthetic electron transport. But, according to the quenching experiment active oxygen species induced lipid peroxidation may be different species in the case of chlorophyll bleaching.