• BMB Reports
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• v.48 no.3
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• pp.147-152
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• 2015

Small interfering RNA (siRNA) functions through pairing with specific mRNA sequences and results in the mRNA's degradation. It is a potential therapeutic approach for many diseases caused by altered gene expression. The delivery of siRNA is still a major problem due to its rapid degradation in the circulation. Various strategies have been proposed to help with the cellular uptake of siRNA and short or small hairpin RNA (shRNA). Here, we reviewed recently published data regarding local applications of siRNA. Compared with systemic delivery methods, local delivery of siRNA/shRNA has many advantages, such as targeting the specific tissues or organs, mimicking a gene knockout effect, or developing certain diseases models. The eye, brain, and tumor tissues are 'hot' target tissues/organs for local siRNA delivery. The siRNA can be delivered locally, in naked form, with chemical modifications, or in formulations with viral or non-viral vectors, such as liposomes and nanoparticles. This review provides a comprehensive overview of RNAi local administration and potential future applications in clinical treatment.

• Toxicological Research
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• v.3 no.1
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• pp.33-44
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• 1987

A fertility study was carried out in Sprague Daxley rats which have been given the intravenous or intraperitoneal injections of rHuIFN-${\alpha}$A, a commecially available therapeutic agent, at dose levels of $1{\times}10^5$, $4{\times}10^5$ and $1.2{\times}10^6$ I.U/kg/day. Male rats were treated with rHuIFN-${\alpha}$A from 60 days before pairing and until the completion of mating. Femal rats received rHuIFN-${\alpha}$A for 22days prior to mating and up to day of gestation. All pregnant females were sacrificed on day 20 of gestation and all fetuses were examined for abnormalities. Both the male and female animals treated with rHuIFN-${\alpha}$A did not show any abnormal responses. No abnormal signs were seen in reproducibility for the rats treated with rHuIFN-${\alpha}$A. No External, internal and skeletal anomalies attributable to rHuIFN-${\alpha}$A were observed in the fetuses. It was concluded that rHuIFN-${\alpha}A$ had no harmful effect on mating, fertilization, implantation, or embryonic development.

• Journal of Microbiology and Biotechnology
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• v.8 no.6
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• pp.700-704
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• 1998

Each of the recombinant structural genes of Helicobacter pylori urease, ureA and ureB, was cloned and overexpressed as inclusion bodies. Solubilization and renaturation of the inclusion bodies were carried out, to accelerate the pairing of sulfhydryl groups and the incorporation of nickel ions, which would lead to the native structure with high enzyme activity. Rates of urea hydrolysis were monitored as an indication of in vitro activation of renatured ureases. The activation of the apoprotein using 1 mM nickel ion, 100 mM sodium bicarbonate and a 10:1 ratio of reducing power resulted in a weak urease activity (about 11% of the native urease activity encoded by pTZ 19R/ure-l). When a sparse matrix screen method originally discovered for the crystallization of proteins was used, the activity increased higher than that obtained using glutathione. The effect of polyethylene glycol (PEG) on the activity was noticeable, giving two-fold increase in the specific activity (about 11 U/mg of protein corresponding to 22% of the native urease activity encoded by pTZ19R/ure-1).

• International Journal of Aeronautical and Space Sciences
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• v.1 no.1
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• pp.1-12
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• 2000

The wake geometries of a two-bladed rotor in axial flights using a time-marching free-wake method without a non-physical model of the far wake are calculated. The computed free-wake geometries of AH-1G model rotor in climb flight are compared with the experimental visualization results. The time-marching free-wake method can predict the behavior of the tip vortex and the wake roil-up phenomena with remarkable agreements. Tip vortices shed from the two-bladed rotor can interact with each other significantly. The interaction consists of a turn of the tip vortex from one blade rolling around the tip vortex from the other. Wake expansion of wake geometries in radial direction after the contraction is a result of adjacent tip vortices begging to pair together and spiral about each other. Detailed numerical results show regular pairing phenomenon in the climb flights, the hover at high angle of attack and slow descent flight too. On the contrary, unstable motions of wake are observed numerically in the hover at low angle of attack and fast descent flight. It is because of the inherent wake instability and blade-vortex-interaction rather then the effect of recirculation due to the experimental equipment.

• Bulletin of the Korean Chemical Society
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• v.29 no.10
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• pp.1937-1940
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• 2008

The P1 duplex of Tetrahymena group I ribozyme is the important system for studying the conformational changes in folding of ribozyme. The formation of the P1 duplex between IGS and substrate RNA and the catalytic activity of ribozyme require a variety of metal ions such as $Mg^{2+}$ and $Mn^{2+}$. In order to investigate the effect of the $Mg^{2+}$ concentration on the conformation of the P1 duplex, the NMR study was performed as a function of $Mg^{2+}$ concentration. This study revealed that the less stable AU-rich region formed duplex at $50{^{\circ}C}$ under high $Mg^{2+}$ concentration condition but melts out under low $Mg^{2+}$ concentration condition. It was also found that in the active conformation under 10 mM $MgCl_2$ condition, the unstable central G${\cdot}$U wobble pair maintains the significant base pairing up to $50{^{\circ}C}$. This study provides the information of the unique feature of the P1 duplex structure and the roll of $Mg^{2+}$ ion on the formation of the active conformation.

• Bulletin of the Korean Chemical Society
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• v.29 no.1
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• pp.117-121
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• 2008

Pseudo-first-order rate constants (kobsd) have been measured spectrophotometrically for nucleophilic substitution reactions of 5-nitro-8-quinolyl benzoate (5) with alkali metal ethoxides, EtO?M+ (M+ = Li+, Na+ and K+) in anhydrous ethanol (EtOH) at 25.0 0.1 C. The plots of kobsd vs. [EtO?M+] exhibit upward curvatures, while the corresponding plots for the reactions of 5 with EtO?Na+ and EtO?K+ in the presence of complexing agents, 15-crown-5-ether and 18-crown-6-ether are linear with rate retardation. The reactions of 5 with EtO?Na+ and EtO?Li+ result in significant rate enhancements on additions of Na+ClO4, indicating that the M+ ions behave as a catalyst. The dissociated EtO and ion-paired EtOM+ have been proposed to react with 5. The second-order rate constants for the reactions with EtO (kEtO) and EtOM+ (kEtOM+) have been calculated from ion-pairing treatments. The kEtO and kEtOM+ values decrease in the order kEtONa+ > kEtOK+ > kEtOLi+ > kEtO, indicating that ion-paired EtOM+ species are more reactive than the dissociated EtO ion, and Na+ ion exhibits the largest catalytic effect. The M+ ions in this study form stronger complex with the transition state than with the ground state. Coordination of the M+ ions with the O and N atoms in the leaving group of 5 has been suggested to be responsible for the catalytic effect shown by the alkali metal ions in this study.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.17 no.5
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• pp.35-42
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• 2017

NOMA (Non-orthogonal multiple access) system is the most promising multiple access technology to satisfy the requirements of the spectral efficiency and the performance of 5G cellular systems. NOMA system simultaneously serves multiple users in the power domain, and adapts SIC (Successive interference cancellation) at the receivers to cancel the interference from multiple users. Since in a realistic wireless fading channel the perfect SIC is impossible, the study of the effect of the imperfect SIC to a NOMA system is necessary. This paper considers a cooperative NOMA system with SIC error, and the performance of the system is analytically derived. And the optimum power allocation to minimize the system performance is obtained. When the transmit power is fixed, the distances between a base station and the relay is considered for different SIC errors. The derived analytical results are verified through Monte Carlo simulation, and the results are perfectly matched.

• Journal of the Korean Society of Propulsion Engineers
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• v.1 no.1
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• pp.33-45
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• 1997

Axisymmetric shear layers around a free jet is forced by co-flowing and counter-flowing secondary jets from/to an annular tube around the jet nozzle. The jet potential core extends far downstream with co-flowing secondary jets due to inhibited vortex developing and pairing. For counter-flowing cases, the axisymmetric shear layer around the jet transits from convective instability to absolute instability for velocity ratios R＝1.3～l.65 for the uniform velocity jets. Consequently, the jet potential core length increases and the turbulence level in the jet core is reduced significantly. The jets are controlled better with extension collars attached to the outer nozzle exit because the annular secondary flow is guided well by the extension collars. For the vectoring of jet, the annular tube around the jet is divided in two parts and the only one part is used for suction. The half suction makes the different shear layer around the jet and vectoring the jet by Coanda effect. The vectoring and turbulent components are varied significantly by the suction ratio. The experiments are carried out to investigate the characteristics of forced free jets using flow visualization, velocity and turbulence measurements.

• KSBB Journal
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• v.16 no.1
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• pp.66-70
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• 2001

Factors affecting the supercritical carbon dioxide extraction of camptothecin(CPT) and 10-hydroxycamptothecin(HCPT) from the dried powder of Cmptotheca acuminata were studied. Only a few amount of CPT and HCPT was extracted with pure supercritical carbon dioxide. Methanol and ethanol were efficient modifiers to extract CPT and HCPT. At $40^{\circ}C$, 250 bar, 1 mL/min flow rate, 41% of CPT and 35% of HCPT were extracted with supercritical carbon dioxide modified with 16% of methanol. The diffusion effect of HCPT on extraction efficiency was studied in this solid-fluid system. Round matrix hot-ball model assumption revealed that the value of D/$r^2$ was 0.0072 $min^{-1}$ which was higher than that of solvent extraction with methanol.

• Journal of the Korean Institute of Intelligent Systems
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• v.15 no.1
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• pp.24-29
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• 2005

In the fuzzy control for the multi-variable system, it is difficult to obtain the fuzzy rule. Therefore, the parallel structure of the independent single input-single output fuzzy controller using a pairing between the input and output variable is applied to the multi-variable system. However, among the input/output variables which arc not paired the interactive effects should be taken into account. these mutual coupling of variables affect the control performance. Therefore, for the control system with a strong coupling property, the control performance is sometimes lowered. In this paper, the effect of mutual coupling of variables is considered by the introduction of a neuro-fuzzy controller using relative gain matrix. This proposed neuro-fuzzy controller automatically adjusts the mutual coupling weight between variables using a neural network which is realized by back-propagation algorithm. The good performance of the proposed nero-fuzzy controller is verified through computer simulations on 200MW boiler systems.