• Applied Chemistry for Engineering
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• v.27 no.1
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• pp.1-9
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• 2016

The co-culture system of microalgae and bacteria enables simultaneous removal of BOD and nutrients in a single reactor if the pair of microorganisms is symbiotic. In this case, nutrients are converted to biomass constituents of microalgae. This review highlights the importance and recent researches using symbiotic co-culture system of microalgae and bacteria in wastewater treatment, focusing on the removal of nitrogen and phosphorus. During wastewater treatment, the microalgae produces molecular oxygen through photosynthesis, which can be used as an electron acceptor by aerobic bacteria to degrade organic pollutants. The released $CO_2$ during the bacterial mineralization can then be consumed by microalgae as a carbon source in photosynthesis. Microalgae and bacteria in the co-culture system could cooperate or compete each other for resources. In the context of wastewater treatment, positive relationships are prerequisite to accomplish the sustainable removal of nutrients. Therefore, the selection of compatible species is very important if the co-culture has to be utilized in wastewater treatment.

• Journal of Animal Reproduction and Biotechnology
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• v.34 no.3
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• pp.148-156
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• 2019

The Transgenic livestock can be useful for the production of disease-resistant animals, pigs for xenotranplantation, animal bioreactor for therapeutic recombinant proteins and disease model animals. Previously, conventional methods without using artificial nuclease-dependent DNA cleavage system were used to produce such transgenic livestock, but their efficiency is known to be low. In the last decade, the development of artificial nucleases such as zinc-finger necleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas has led to more efficient production of knock-out and knock-in transgenic livestock. However, production of knock-in livestock is poor. In mouse, genetically modified mice are produced by coinjecting a pair of knock-in vector, which is a donor DNA, with a artificial nuclease in a pronuclear fertilized egg, but not in livestock. Gene targeting efficiency has been increased with the use of artificial nucleases, but the knock-in efficiency is still low in livestock. In many research now, somatic cell nuclear transfer (SCNT) methods used after selection of cell transfected with artificial nuclease for production of transgenic livestock. In particular, it is necessary to develop a system capable of producing transgenic livestock more efficiently by co-injection of artificial nuclease and knock-in vectors into fertilized eggs.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.403-406
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• 2005

In order to evaluate the Capability of ETM+ remotely- sensed data to provide 'Forest-shrub land-Rangeland' cover type map in areas near the timberline of northern forests of Iran, the data were analyzed in a portion of nearly 790 ha located in Neka-Zalemroud region. First, ortho-rectification process was used to correct the geometric errors of the image, yielding 0/68 and 0/69 pixels of RMS. error in X and Y axis, respectively. The original and panchromatic bands were fused using PANSHARP Statistical module. The ground truth map was made using 1 ha field plots in a systematic-random sampling grid, and vegetative form of trees, shrubs and rangelands was recorded as a criteria to name the plots. A set of channels including original bands, NDVI and IR/R indices and first components of PCI from visible and infrared bands, was used for classification procedure. Pair-wise divergence through CHNSEL command was used, In order to evaluate the separability of classes and selection of optimal channels. Classification was performed using ML classifier, on both original and fused data sets. Showing the best results of $67\%$ of overall accuracy, and 0/43 of Kappa coefficient in original data set. Due to the results represented above, it's concluded that ETM+ data has an intermediate capability to fulfill the spectral variations of three form- based classes over the study area.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.1
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• pp.85-100
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• 2016

Local Agenda 21 organizations have been founded at the most divisions of local government in Chungcheongbuk-Do, but there has been no methods to monitor the total Sustainable Development trends. For the alternative solution of that problem in the Province, this study purposed (1) to develop Indicators fit to this Province (2) to examine the availability of the developed indicators adopting to each division section. Indicators were developed and examined by fields and Quantity / Quality considerations. As a 1st step, committee-composed with field professionals each-selected and developed Quantitative Sustainable Development Indicators(SDIs) optimized to Chungbuk-Province through critical discussions proposing Qualitative Indicators as a suggestion. In 2nd step, developed Indicators were applied and tested upon each divisions. Statistic data were used to calculate indices. 3rd step is adoption stage of Qualitative Indicators which were tested with questionnaire survey analysis. As a results, SDIs for Chungcheongbuk-Do -38 Quantitative SDIs within 4 provisions of 11 items with 8 Qualitative indicators categorization be put off- is developed. With the application test, 5 Quantitative Indicators- were found to be not adaptable because of lack or none of statistic data. Among the 8 Qualitative Indicators, 3 pairs found to be similar results in each pair which needed to be integrated into one. With them, bio-species related and health related Indicators were proposed to be newly added. Nation wide Sustainability comparison between divisions and Ecological Indicator selection are another themes to be considered.

• Korean Journal of Food Science and Technology
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• v.47 no.5
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• pp.608-614
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• 2015

Four Korean commercial rice wines of diverse sensory properties were hedonically rated by 41 consumers. Each rice wine was paired with the selection from six widely popular compatible foods such as kimchi-jjigae, dotori-muk muchim, tteok-galbi, beoseot-jeongol, satae pyeonyuk, and haemul pajeon, and consumers were asked to rate the ideal match of each pair of four rice wines and six compatible foods by using a structured, 12-cm 'just right' line scale. Hedonic ratings of wines were additionally measured after intake of the food-wine pairs. Flavor matches of rice wines with strong ginseng, medicinal, and earthy flavor (JK) or with intense grain and sweet flavor (HS) were significantly higher compared to wines with fruity (SS) or mild-balanced (BS) flavor. The preference for HS wine, which ideally matched satae pyeonyuk, increased most significantly after intake of satae pyeonyuk, while the SS wine that matched ideally with kimchi jjigae did not show any significant increase in preference after intake of kimchi jjigae. Matching wines with food does not negatively affect the preference for the wine; rather, intake of a wine-food pairing increases the overall preference for rice wine.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1607-1615
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• 2007

We investigated the applicability of the TEM-l ${\beta}$-lactamase fragment complementation (BFC) system to develop a strategy for the screening of protein-protein interactions in bacteria. A BFC system containing a human Fas-associated death domain (hFADD) and human Fas death domain (hFasDD) was generated. The hFADD-hFasDD interaction was verified by cell survivability in ampicillin-containing medium and the colorimetric change of nitrocefin. It was also confirmed by His pull-down assay using cell lysates obtained in selection steps. A coiled-coil helix coiled-coil domain-containing protein 5 (CHCH5) was identified as an interacting protein of human uracil DNA glycosylase (hUNG) from the bacterial BFC cDNA library strategy. The interaction between hUNG and CHCH5 was further confirmed with immunoprecipitation using a mammalian expression system. CHCH5 enhanced the DNA glycosylase activity of hUNG to remove uracil from DNA duplexes containing a U/G mismatch pair. These results suggest that the bacterial BFC cDNA library strategy can be effectively used to identify interacting protein pairs.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.10
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• pp.1375-1378
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• 2005

The objective of this study was to investigate polymorphisms of BMPRIB (bone morphogenetic protein type IB receptor) gene and its effect on litter size traits in sheep. Three populations including 101 Small Tailed Han sheep, 79 Poll Dorset and 81 hybrids (Poll Dorset${\times}$Small Tailed Han sheep) were used to detect the polymorphisms in exon 6 region of sheep BMPRIB gene. A fragment of approximately 190bp was amplified by one pair of primers, the polymorphism was revealed from the analysis of three populations by the technique of PCR -SSCP, and a mutation from A to G at 746 of the coding region was confirmed by sequencing in several individual. Statistical results indicated the distribution of allele B (with a A$\longrightarrow$G mutation) and A (without mutation) or genotype AA, AB and BB frequencies differed in three populations. BB genotype (44.55%) and B allele (66.34%) frequencies of Small Tailed Han sheep were higher than those of the others. Analysis of variance showed that the polymorphism of BMPRIB gene was associated with positive effect on litter size traits. The means of genotype BB and AB were about 1.04 and 0.74 more than genotype AA for litter size (p<0.05). Analysis of BMPRIB genotype effects on litter size in three populations indicates the existence of genotype BB or B allele increases the litter size. It suggested that the polymorphism in exon 6 (at 746 in the coding region) of sheep BMPRIB gene may be used as a marker for early selection of prolificacy in sheep.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.8
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• pp.3028-3035
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• 2010

This paper propose a genetic method based on probability map to detect region of the lips on a natural image with the faces. The method has many solutions in order to detect regions such as the lips instead of one optimal solution of existing methods. To do this, it represents a pair of spatial coordinates as a chromosome, and introduces genetic operations like conservation interval, the number of generations and non-overlapping selection. By using the probability map of the HS in HSV color space, it increases adaptability to similar color that is a property of genetic algorithm. In our experiments, the optimal value of the important parameter $\beta$ was analyzed, which was used as the condition of an ending function and affected performance of the proposed algorithm. Also the algorithm was analyzed on what performance it has when its mating methods are different. The results of the experiment showed that our algorithm could be flexibly adapted for detecting other ROIs.

• Journal of KIISE:Software and Applications
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• v.35 no.11
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• pp.681-691
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• 2008

In this paper, we have evaluated and compared each feature and feature combinations necessary for statistical Korean dialogue act classification. We have implemented a Korean dialogue act classification system by using the Support Vector Machine method. The experimental results show that the POS bigram does not work well and the morpheme-POS pair and other features can be complementary to each other. In addition, a small number of features, which are selected by a feature selection technique such as chi-square, are enough to show steady performance of dialogue act classification. We also found that the last eojeol plays an important role in classifying an entire sentence, and that Korean characteristics such as free order and frequent subject ellipsis can affect the performance of dialogue act classification.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.5
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• pp.734-740
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• 2005

The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax-$\alpha$ at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-$\alpha$. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-$\alpha$mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.