• Journal of Korea Society of Digital Industry and Information Management
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• 제12권4호
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• pp.71-79
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• 2016

This study proposes a performance model of a shared bus multi-processor system and analyzes the effect of input/output types on system performance and overload of shared resources. This system performance model reflects the memory reference time in relation to the effect of input/output types on shared resources and the input/output processing time in relation to the input/output processor, disk buffer, and device standby places. In addition, it demonstrates the contribution of input/output types to system performance for comprehensive analysis of system performance. As the concept of workload in the probability theory and the presented model are utilized, the result of operating and analyzing the model in various conditions of processor capability, cache miss ratio, page fault ratio, disk buffer hit ratio (input/output processor and controller), memory access time, and input/output block size. A simulation is conducted to verify the analysis result.

• Microbiology and Biotechnology Letters
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• 제24권2호
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• pp.173-177
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• 1996

Bacillus thuringiensis subsp. morrisoni PG-14 is a gram-positive soil bacterium producing mosquitocidal parasporal inclusions composed of several crystal proteins. Among these crystal protein genes, cryIVD gene is one of major component which has 72 kDa in size. However, these parasporal inclusions sink quickly from the surface of water where mosquito larval feeding occurred. To develope mosquitocidal cyanobacterium, therefore, we constructed the expression vector, pCYASK 5-1 harboring cryIVD gene. The expression vector, pCYASK5-1 was transformed into the cyanobacterium Syne- chocystis PCC6803 reported as a natural mosquito larval food source and the transformants were selected with kanamycin. Expression of IVD gene in transformant was characterized by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined with Culex tritaeniorhynchus. The results showed that, the transformed cyanobacterium is toxic to mosquito larvae and will be expected as a potential agent that is used for mosquito control.

• Korean Journal of Human Ecology
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• 제8권3호
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• pp.511-524
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• 1999

This research examines 13 Korean Web sites and 15 foreign Web Sites to explore how companies present apparel products by both layout of graphics and information at the Web sites. The results show that most Web sites display tiny icons next to the item's text description. Clicking on these icons takes the customers to another web page, where the full size photograph of the item appears. The results also revealed that most web sites offer shopping bag function and payment options such as on-line and credit cards. The results indicate that Web sites are constantly evolving and following functions such as virtual dressing room, FAQ, the links, E-Cash payment, currency converter and multilingual sites are becoming standards in the near future.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 한국식품저장유통학회 2003년도 제23차 추계총회 및 국제학술심포지움
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• pp.135.1-135
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• 2003

To elucidate the effect of gamma-irradiation on the molecular properties of hemoglobin, the secondary, tertiary structure, and the molecular weight size of the protein were examined after irradiation at 0.5, 1, 5, and 10 kGy. Gamma-irradiation of hemoglobin solutions caused the disruption of the ordered structure of the protein molecules, as well as degradation, cross-linking, and aggregation of the polypeptide chains. A SDS-PAGE study indicated that irradiation caused initial fragmentation of the proteins and subsequent aggregation due to cross-linking of the protein molecules. The effect of irradiation on the protein was more significant at lower protein concentrations. Ascorbic acid decreased the degradation and aggregation of proteins by scavenging oxygen radicals that were produced by irradiation. A circular dichroism study showed that irradiation decreased the helical content of hemoglobin with a concurrent increase of the aperiodic structure content. Fluorescence spectroscopy indicated that irradiation decreased the emission intensity that was excited at 280 nm.

• Proceedings of the Korean Information Science Society Conference
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• 한국정보과학회 2003년도 가을 학술발표논문집 Vol.30 No.2 (2)
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• pp.259-261
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• 2003

소프트웨어 시스템의 규모 측정은 소프트웨어 개발에 필요한 비용을 산정하거나 필요한 인력 투입. 기간의 산정 등의 중요한 지표가 된다. 1990년대 후반 급격한 인터넷의 발달로 인해 다양한 기술과 웹 사이트가 등장하였다. 기존의 소프트웨어의 규모를 측정하기 위한 방법은 LOC와 기능점수(Function Point)가 있다. 그러나 LOC는 프로그래밍 언어에 의존적이므로 웹 페이지 규모를 측정하기는 한계가 있다. 따라서 웹 페이지 규모를 측정하기 위해 기능점수분석을 사용한다. 본 논문에서는 동일한 웹 페이지를 기존의 기능점수모형과 확장된 기능점수모형으로 분석하여 그 결과를 비교하고 미비점을 보완한다. 또한 다양한 웹 페이지들의 규모를 측정하기 위하여 기존 기능점수분석모형에 추가 조정 인자를 기술하고 확장된 기능점수모형을 제안한다.

• Proceedings of the KIEE Conference
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• 대한전기학회 2006년도 제37회 하계학술대회 논문집 C
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• pp.1669-1670
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• 2006

LED have many problem as narrow light view angle, high price, drift phenomenon of color coordinate, high heating problem for lower power, lower weight and small size. But, with the development of blue LEDs and the improvement of LED brightness, color variation are becoming more and more popular as lighting and application devices in this page, 1 used the Light Tools which was a simulation program in order to recognize optical a few characteristic of LEDs arrangement along reflector matrial and designed LED Arranged 5 phi LED in 5mm interval In the square that was $10{\times}10$ And designs a reflection board on pcb, structural design of PCB changed as five type. - Aluminum, Gold. Chromium, Nickel, Copper.

• Journal of Microbiology
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• 제43권5호
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• pp.437-442
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• 2005

Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on $13\%$ SDS-PAGE. The sequence of the first 26 amino acid residues from the N-terminal end of the protein is AELYNQDGTSLDMGGRAEARLSMKDG, which is a perfect match with OmpU of V. vulnificus CMCP6 and YJ016. MOMP specific IgM and IgG were investigated in groups of mice. The group of mice immunized with MOMP and Alum showed higher levels of IgG2b than the group immunized with only MOMP. Vaccination with MOMP resulted in protective antibodies in the mouse infection experiment.

• Parasites, Hosts and Diseases
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• 제36권4호
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• pp.255-260
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• 1998

To evaluate the biological and biochemical characteristics of Trichomonas vaginalis KT9 isolate, the growth and size of trichomonads, pathogenicity in mouse, protein profiles and proteinase activity were examined after shifting the medium from TPS-1 into TYM. Generation time of trichomonads in TYM medium was 4.5 hr in comparison to TPS-1 with 7.1 hr. Size of trichomonads cultured in TPS-1 medium ($8.5{\;}{\pm}{\;}0.9{\;}{\times}{\;}6.0{\;}{\pm}{\;}0.9{\;}{\mu\textrm{m}}$) was significantly smaller than those in TYM medium ($10.9{\;}{\pm}{\;}1.4{\;}{\times}{\;}8.2{\;}{\pm}{\;}0.9{\;}{\mu\textrm{m}}$). Trichomonads cultured in TYM medium produced subcutaneous abscess in 9 out of 10 mice, whereas those in TPS-1 medium produced abscesses in 2 out of 10 mice. In SDS-PAGE, trichomonad Iysates from both media showed ten common bands. However, trichomonads in TYM medium showed additional bands of 136 kDa, 116 kDa and 40 kDa in comparison to those in TPS-1 with 100 kDa. By immunoblot with T. vaginalis-immunized rabbit sera, T. vaginalis cultivated in both TYM and TPS-1 media showed 5 common bands. and unique bands of 116 kDa. 105 kDa. and 86 kDa were observed in trichomonads in TYM while a 140 kDa band in those in TPS-1. In gelatin SDS-PAGE, trichomonads in TYM degraded gelatin stronger than those in TPS-1. Also protease activity of trichomonads in TYM was significantly higher than that of trichomonads in TPS-1 using Bz-Pro-Phe-Arg-Nan as a substrate. According to the results, it is assumed that the shift from TPS-1 into TYM medium for cultivation of T. vaginalis might modulate the biological and biochemical properties of T vaginalis in vitro.

• Korean Journal of Microbiology
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• 제54권4호
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• pp.330-342
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• 2018

The Streptavidin and Biotin system has been studied most extensively as the high affinity non-covalent binding of Biotin to STR ($K_D=10^{-14}M$) and four Biotin binding sites in tetrameric Streptavidin makes this system useful for the production of multivalent antibody. For the application of this system, we cloned Streptavidin amplified from Streptomyces avidinii chromosome by PCR and fused to gene of hAY4 single-chain Fv antibody specific to death receptor 4 (DR4) which is a receptor for tumor necrosis factor ${\alpha}$ related apoptosis induced ligand. The hAY4 single-chain Fv antibody fused to Streptavidin expressed in Escherichia coli showed 43 kDa monomer in heated SDS-PAGE. However, this fusion protein shown in both non-heated SDS-PAGE and Size-exclusion chromatography exhibited 172 kDa as a tetramer suggesting that natural tetramerization of Streptavidin by non-covalent association induced hAY4 single-chain Fv tetramerization. This fusion protein retained a Biotin binding activity similar to natural Streptavidin as shown in Ouchterlony assay and ELISA. Death receptor 4 antigen binding activity of purified hAY4 single-chain Fv fused to Streptavidin was also confirmed by ELISA and Westernblot. In addition, surface plasmon resonance analysis showed 60-fold higher antigen binding affinity of the hAY4-STR than monomeric hAY4 ScFv due to tetramerization. In summary, hAY4 single-chain Fv fused to Streptavidin fusion protein was successfully expressed and purified as a soluble tetramer in E. coli and showed both Biotin and DR4 antigen binding activity suggesting possible production of bifunctional and tetrameric ScFv antibody.

• Journal of Digital Convergence
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• 제15권10호
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• pp.93-101
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• 2017

This study investigate the effect of each type of search volume on the number of Facebook fans and the number of tourists. According to the hierarchy effect model, the effect of communication appears to be the sequentiality of cognition-attitude-behavior. Applying this theory, this study predicted that when consumers who have higher involvement and knowledge on specific cities through search behavior, they will be more active in information search through Facebook fan page subscription and will lead to direct tourism behavior. To verify the prediction, we examined the influences among search volume of Seoul shown in Google Trend, the number of fans of official facebook page named 'Seoul Korea', and the number of foreign tourists. As a result, the type of search keyword was divided into four categories: tourism attraction keyword, natural environment keyword, symbolic keyword, and accessibility keyword. The regression analysis showed that tourism attraction keyword and symbolic keyword have influence on Facebook fanpage 'Like'. In addition, facebook fanpage fan size have mediation effect between search volume and number of tourists. All in all, it would be useful to appeal to foreign tourists with a message that emphasizes tourism attraction and Korea-related contents.