• 한국작물학회지
• /
• 제45권2호
• /
• pp.83-87
• /
• 2000

The five phenolic compounds of peony root were isolated by Sephadex LH-20 column chromatography. Their chemical structures were identified by spectroscopic methods (UV, FT-IR, FAB-MS and $^1H$ .&^13C$-NMR). The complete structures of these compounds were elucidated to be (+)-taxifolin-3-0-$\beta$-D-glucopyranoside, benzoic acid, gallic acid, (-)-epicatechin and (+)-catechin. The concentrations of five phenolic compounds in the peony root of three Korean cultivars (Youngchonjakyak, Euisungjakyak and Jomjakyak) were determined by reverse-phase HPLC. The constituents concentration in Youngchonjakyak were generally higher than in Euisungjakyak and Jomjakyak. The concentrations of (+)-taxifolin-3-O-$\beta$-D-glucopyranoside, benzoic acid, gallic acid, (-)-epicatechin and (+)-catechin in three different cultivars were ranged 0.23-0.52%, 0.20-0.30%, 0.26-0.28%, 0.09-0.12% and 0.34-0.63%, respectively.

• 식물조직배양학회지
• /
• 제22권1호
• /
• pp.13-17
• /
• 1995

작약의 화분배양에 의한 반수체 식물을 유기시킬 목적으로 화분배양을 실시하여 배양중 소포자의 변화를 관찰하고 캘러스 및 배발생에 미치는 저온전처리(5$^{\circ}C$/10 days)의 효과와 배양화분의 생존율을 조사하였다. 작약의 화분배양시 캘러스 및 배발생에는 저온전처리의 효과가 다소 인정되었으나 약배양의 효과에는 미치지 못하였다. 화분를 첨가한 고체배지에 이식했을 때 구형배가 형성되었다. 화분의 생존율에는 저온전처리 및 배지조성간 의 영향이 미치지 않았으며 배양기간이 지남에 따라 화분의 생존율도 저하되었다.

• Current Research on Agriculture and Life Sciences
• /
• 제12권
• /
• pp.51-55
• /
• 1994

작약(芍藥)의 약(葯)으로부터 분리된 화분소포자(花粉小胞子)를 생장조절제가 첨가되지 않은 MS 배지에 배양하였을때 직접 배(胚)발생 과정을 거쳐서 소포자 유래의 배(胚)가 형성되었고, 1mg/l의 PAA가 첨가된 배지에서는 화분소포자 유래의 캘러스가 형성 되었으며 이들 캘러스를 생장조절제를 함유하지 않은 배지에 이식하였을 때 배양 7주 후에 배(胚)가 형성되었다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
• /
• pp.737-740
• /
• 2000

Two groups of bacteria, harmful and useful bacteria, coexist in our body. Harmful bacteria and free radicals which cause many diseases and agings could be controlled by not synthetic materials but natural products. 50 kinds of natural products were searched for this purpose, some of them expressed bacterial controlling ability. Clostridium difficle was controlled by Paeonia Japonica and Lycii Fructus, etc., Clostridium perfringens was inhibited by Scutellaria Baicalensis and Astragali Radix, etc., and same way, Listeria monocytogenes; Paeonia Japonica and Scutellaria Baicalensis, E. coli; Scutellaria Baicalensis and Schizandrae Fructus, Staphylococcus aureus; Paeonla Japonica and Scutellaria Baicalensis, Streptococcus mutans; Coptis Rhizoma, respectively. These natural products will play an important role in bio-Industry.

• 생약학회지
• /
• 제33권2호통권129호
• /
• pp.144-150
• /
• 2002

The methanol extracts of 132 herbal medicines were screened for the inhibitory activity against heparinase enzyme from Flavobacterium heparinum. Eleven medicinal plants, Amomum xanthiodides, Agrimonia pilosa, Paeonia lactiflora, Rubia cordifolia, Sanguisorba officinalis, Torrega grandis, Morus alba, Gleditsia sinensis, Crataegus pinnatifida, Cornus officinalis, Paeonia japonica showed potent inhibition on heparinase activity. The active substituents of those herbal medicine could be extracted into butanol fraction and the inhibitory compounds of Morus alba are now isolating.

• 한국식물병리학회지
• /
• 제13권1호
• /
• pp.1-4
• /
• 1997

Viroid-like RNA molecules were detected from the low molecular weight RNAs isolated from the Korean peonies which showed typical viroid symptoms of epinasty and dwarfing. Low molecular weight RNAs including viroid RNA molecules were purified by the Qiagen anion exchange minicolumns. Viroid-like RNA molecules showed a single viroid specific band in the native polyacrylamide gel. They were separated into two bands in the denaturing gel conditions. The band of circular form of viroid-like RNAs was crossed over the horizontal band of the linear form of viroid-like RNA molecules in 0~8 M urea gradient gel under the denaturing conditions of 37$^{\circ}C$. The two circular forms of viroid-like RNA molecules were detected in the reverse polyacrylamide gel electrophoresis. The viroid-like RNA molecules purified from the peonies were supposed to be unidentified viroid RNA molecules.

• 한국작물학회지
• /
• 제49권4호
• /
• pp.354-357
• /
• 2004

A simplified technique which cryoprotects zygotic embryos by encapsulation-dehydration was developed for the germplasm conservation of herbaceous peony (Paeonia lactiflora Pall.). The highest survival rate $(85\%)$ was obtained from embryos treated by encapsulation-dehydration. The zygotic embryos were precultured on MS medium containing 0.3mg/L $GA_3$ for 1 day. The precultured embryos were encapsulated in $3\%$ (w/v) alginate beads and immersed for 1 h in MS medium containing 2 M glycerol and 0.5 M sucrose. The encapsulated embryos were dehydrated for 5h by air drying prior to direct immersion in liquid nitrogen. This encapsulation-dehydration method appears to be a promising technique for germplasm cryopreservation of a herbaceous peony.

• 대한본초학회지
• /
• 제21권3호
• /
• pp.1-6
• /
• 2006

Objectives : In this report, we were studied the current status and future about cultivation of medicinal plants in Japan. Methods : To analysis the management and production of medicinal crops in Japan, variation of importation and cultivation of four medicinal crops such as Angelica acutiloba Kitagawa, Cnidium officinale Makino, Paeonia lactiflora Pallas and Rehmannia glutinosa Liboschitz var. purpurea Makino within 22 years ($1981{\sim}2002$) were investigated. Also we analyzed the herbal drug market in Japan using literature. Results : In the results, cultivated area and amount of Angelica acutiloba Kitagawa and Paeonia lactiflora Pallas reduced more than 50% within 22 years ($1981{\sim}2002$). Production decrease of medicinal crops is alloting through contract production in China. The cultivation of Rehmannia glutinosa Liboschitz var. purpurea Makino is enforcing minimum procluction for seed security. Conclusion : We hope that this report stimulates research to investigate for the establishment of cultural practices in Korea.

• 생약학회지
• /
• 제39권1호
• /
• pp.37-42
• /
• 2008

From the 70% EtOH extract from the roots of Paeonia lactiflora (Paeoniaceae), nine triterpenoids were isolated and identified as ${\beta}-amyrin$ (1), 24-methylenecycloartanol (2), betulinic acid (3), oleanolic acid (4), hederagenin (5), 30-norhederagenin (6), 30-norarjunolic acid (7), arjunolic acid (8), and $3{\beta},4{\beta},23-trihydroxy-24,30-dinorolean-12,20(29)-dien-28-oic$ acid (9) by spectroscopic methods. Among these compounds, 1, 2, 7, 8 and 9 were isolated for the first time from this plant.

• 생약학회지
• /
• 제39권1호
• /
• pp.28-36
• /
• 2008

From the 70% EtOH extract of Paeonia lactiflora roots (Paeoniaceae), fourteen phenolic and related compounds were isolated. They were identified as ${\alpha}-tocopherol$ (1), dioctylphthalate (2), ${\alpha}-tocospiro$ B (3), paeonol (4), 3,3'-di-O-methylellagic acid(5), 3,4'-di-O-methylellagic acid (6), benzoic acid (7), aromadendrin (8), p-hydroxybenzoic acid (9), (+)-catechin (10), gallic acid (11), nicotinamide (12), methyl gallate (13) and $1,2,3,4,6-penta-O-galloyl-{\beta}-D-glucose$ (14) by spectroscopic methods. Among these compounds, 1-3, 5, 6, 8 and 12 were isolated for the first time from this plant.