• Korean Journal of Plant Resources
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• v.6 no.2
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• pp.155-163
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• 1993

21plants, which collected from Korea and Japan, were applied to antitumor and cytotoxic screening tests against sarcoma 180 a ascitec in mice. The results are summariged as follows : 1) The total packed cell volume method has been used for the antimeoplastic screening for from natural higher plants in Korea. By this method, we have found out that Selaginella involves, Patrinia hispida, Archyranthes japonica and Solanum nigrum having significant activity and also Cydonia sinensis and Rubia akane showed slight activity to antitumor 2) The total packed cell volune method has been used for the antineoplastic screening for from natural higher plants in Japan. Among the 21 tested plants, lsodon japonicus having strong antitumor activity and also Torilis japonica, Aralia elata, Leonurus sibiricus and Rubia cordifolia showed significant activity to anticancer tumor while Forsythia spp and Solanum nigrum showed slight activity to antitumor. 3) Among the 21 tested Korea plants, lsodon excisus and Forsythia Koreana showed strong antitumor activity by the V79 cytotoxic cell screening test.

• Journal of Veterinary Clinics
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• v.20 no.1
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• pp.110-114
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• 2003

The Korean Chinese Chives(Allium tuberosum Rottler) are a perennial herb and have been used from ancient as food and medical purpose in Korea. They are known to posses diuresis, sthemia activities and used for the treatment or anemia, haematemesis, and eczema. Recently the Korean Chinese Chives have been studied on the morphologic property, the classification of origin and chemical constituent. However there has not been studied on kg) (or 6 days and observed the hematological effects in dogs. The results are as follows, 1. The red blood cell count was significantly (P < 0.05) decreased on day 2. 2. The reduced glutathion(GSH) concentration was significantly (P< 0.05) increased on day 4 and Met-hemoglobin concentration was significantly (P< 0.05) increased on 3 hrs after administration. 3. Packed cell volume(PCV) and hemoglobin concentration were not showed significant difference during experimental periods. 4. The mean corpuscular volume(MCV) was significantly(P < 0.05) increased on 3 hours after administration.

• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.549-557
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• 1994

This study was done to find the method of the extermination of Fasciola hepatica matacercariae. And the artificial infection was carried out with 30 metacercarae exposed to 5% ammonia water and not-exposed to 5% ammonia water. Serial determinations of live weight, red blood cell, hemoglobin, packed cell volume, and eosinophils were performed in rats at 7 days interval for 16 weeks after infection (WAI). Recovery of worm burden and microscopic findings of livr was performed in rats at 10 WAI. The results in this work were summarized as follows; 1. Fasciola spp metacercariae exposed to 5% ammonia water have lost their ability of infection. 2. In teh exposed group, the mean of worm recovered was 2.25 and the common bile duct was swelling up to 0.71cm in diameter. 3. The value of live weight was different in two groups as the not-exposed group and the exposed group were 321.28, 384.38 at 10 WAI, respectively. 4. In the not-exposed group, at 7 WAI, hemoglobin at 5 WAI and packed cell volume at 7 WAI wre minimally decreased to $5.84{\times}10^{-6}/mm^3$, 11.53g/dl and 43.2%, respectively. But those three values were slowly increased at 10 WAI. Rercent cosinophil was increased to 12.2% at 4 WAI and slightly decreased to 7.9% at 10 WAI. But there are no stastistical singnificance between the exposed group and the normal control group. 5. In histolgical findings in the not-exposed group, the dilated common bile ducts and intrahepatic bile ducts showed distinct hyperplasia of the epithelium. Lymphocytes and eosinophils were infilterated around the bile ducts. The hepatic cells and Kupffer cells showed swollen appearance.

• Journal of environmental and Sanitary engineering
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• v.11 no.3
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• pp.13-19
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• 1996

Packed bed reactor containing immobilized microorganisms which degraded phenol without growth was used to remove phenol from the synthetic wastewater. The effects of temperature, retention time(reactor volume/flow rate) and phenol concentration on the removal efficiency of phenol were investigated. The effect of temperature in the range of 20-30$\circ $C was negligible while retention time and phenol concentration influenced the removal of phenol significantly. When retention time was in the range of 1-1.5 hour, the removal efficiency of phenol was affected not by phenol concentration but by retention time itself while it was influenced by phenol concentration above 1.5 hour of retention time. The beads after 720 hours operation were swelled by 40 % in diameter which could be prevented by crosslinking with glutaraldehyde at the expense of cell activity.

• Journal of Veterinary Clinics
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• v.15 no.1
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• pp.193-195
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• 1998

A one-month old Holstein calf with clinical histories of diarrheal weekness and behavioural abnormality revealed clinical findings of anemia and dehydration. The red blood cells were $1.4{\times} 10^{6}/{\mu}l$ and packed red cell volume was 45%. A blood smear showed a large number of circulating Trypanosoma theileri. To treat Trypanosomiasisi the patient received once a daily oral dosage o$\ulcorner$ 10 mg/kg of the Quinidine sulfate including transfusion (200 ml) for 3 days. After 2 weeks, the calf recovered body condition. These results suggest that Administration of Quinidine sulfate in trypanosomiasis may be a useful treatment approach.

• KSBB Journal
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• v.8 no.3
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• pp.193-199
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• 1993

To find out the optimum conditions for indigo cell culture in air-lift bioreactor, effects of media composition including nutrients and precursors of the indigo colorants on the cell growth and characteristics of the cell growth under various cultural conditions were analyzed. Optimum cultural conditions were tested and the growth characteristics were analyzed in external and internal loop type air-lift bioreactors during 14-day culture. Better cell growth was obtained when the inoculum size was higher in the range of 0.5∼2.5% packed cell volume tested. In the sucrose concentration of 2 to 4%, the cell growth was better when the sucrose concentration was 4% (w/w) in both types of reactors. Sucrose was used up in the early stage of exponential phase of growth At the optimum concentration of a Precursor tryptophan at 1 U UW was 3.8 g/l in internal loop bioreactor, and 3.5 g/l in external one after 14 days of cultivation. Addition of indole showed negative effect on cell growth of suspension culture in air-lift biorector culture and cell mass of 2.5 g/l and 2.2 g/l were obtained in external and internal loop bioreactor, respectively. Selected inorganic nitrogen source potassium nitrate showed about 110% increase in cell growth than that of control. DCW was 16.34 g/l under optimum conditions during 14-day cultivation in internal loop bioreactor.

• Advances in Traditional Medicine
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• v.7 no.4
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• pp.426-435
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• 2007

The present study was designed to determine the antitumor and antioxidant properties of methanol extract from the root of Galega purpurea (Papilionaceae) (MEGP) against Ehrlich Ascites Carcinoma (EAC) bearing Swiss albino mice. Acute and short-term toxicity studies were performed initially in order to ascertain the safety of MEGP. The effect of MEGP on the growth of transplantable murine tumor, life span of EAC bearing hosts and simultaneous alterations in the hematological profile and liver biochemical parameters (lipid peroxidation, antioxidant enzymes) were estimated. The MEGP showed decrease in tumor volume, packed cell volume and viable cell count and increases the nonviable cell count and mean survival time thereby increasing life span of EAC tumor bearing mice. Hematological profile reverted to more or less normal levels in extract treated mice. Treatment with MEGP decreased the levels of lipid peroxidation and increased the levels of glutathione, superoxide dismutase and catalase. The results suggested that the methanol extract of Galega purpurea root exhibited antitumor effect by modulating lipid peroxidation and augmenting antioxidant defense system in EAC bearing mice.

• Journal of Veterinary Clinics
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• v.19 no.1
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• pp.55-60
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• 2002

The Allium tuberosum is a perennial herb. From the ancient times, it has been used for food or medical purpose. Allium tuberosum is widely distributed in Korea, China, Japan and other countries. As herbal medicine, the Allium tuberosum has medical effects on lumbago, sthenia, diuresis, nocturia, hematemesis, anemia, haemorrhoid, hiccups, diarrhea, eczema, sunstroke, pollution and so on. Recently, the Allium tuberosum has been studied on its components, anti-microorganism action, anti-fungal action, anti-arteriosclerosis action, anti-cancer action. However, there has not been any study on hematological effect of allium tuberosum extract on the blood in large dogs whereas the effects of excess garlic and onion on the blood of cattle, horses, dogs, cats and sheep has been reported by many researchers. Thus, this study was performed to observe the hematological effect of excessive Allium tuberosum extract administrated in large dogs. 1. The Red Blood Cell(R3C) count was significantly decreased(p<0.05), Packed Cell Volume (PCV) count were significantly decreased(P<0.05), and Reticulocyte count was significantly increased(p<0.05) 2. The White Blood Cell(WBC) count was significantly increased(p<0.05).3. The Hemoglobin concentration was significantly decreased(p<0.05) and Mean Corpuscular Hemoglobin Concentration (MCHC) was increased. and Mean Corpuscular Volume(MCV) was significantly increased.(p<0.05). 4. The Reduced glutathione(GSH) and Met-Hemoglobin were significantly increased (p<0.05).

• Korean Journal of Veterinary Research
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• v.33 no.2
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• pp.327-336
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• 1993

Present experiments were undertaken in order to clarify the effect of splenectomy on the hematology and marrow megakaryocyte picture and to know the genesis of postsplenectomy thrombocytosis in dogs. Six mongrel dogs weighing 8.5~18㎏ were used, of which three were splenectomized and the other three were laparotomized for comparison. Erythrocyte count, total and differential leukocyte counts, thrombocyte count and packed cell volume measurement were made using the blood samples. In addition, bone marrow samples obtained from the femur at 7th and 23rd day of the operation were examined for the number per low-power field, the diameter, and the distribution frequency of the megakaryocyte. From these experiments, following results were obtained : Erythrocyte count and packed cell volume showed significant decrease beginning on the 15th day of splenectomy. Total and differential leukocyte counts showed marked increase for the first 2 days of postsplenectomy. The thrombocyte count of splenectomized dogs increased from the 2nd day of the operation, reached to the peak count on the 15th day, and returned to the preoperation count by the 28th day. The megakaryocyte count per low-power field of the biopsied preparation increased in according to the increase in thrombocyte count. The megakaryocyte diameter of splenectomized dog showed no increase on the 7th or 23rd day of the operation. However, the distribution frequency of the larger megakaryocyte was higher in the splenectomized dogs than in the laparotomized dogs. The total plasma protein concentration showed no significant change after splenectomy or laparotomy. From these results, it may be concluded that the postsplenectomy thrombocytosis results from the increased megakaryocytopoesis or the activated thrombocytopoesis of the marrow megakaryocytes.

• Transactions of the Korean hydrogen and new energy society
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• v.26 no.5
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• pp.445-452
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• 2015

A tubular packed bed reactor for the steam-$CO_2$ combined reforming of natural gas to produce the synthesis gas of a target $H_2/CO$ ratio 2.0 was simulated. The effects of the reactor dimension, the feed gas composition, and the gas feeding temperature upon the possibility of coke formation across the catalyst bed were investigated. For this purpose, 2-dimensional heterogeneous reactor model was used to determine the local gas concentrations and temperatures over the catalyst bed. The thermodynamic potential distribution of coke formation was determined by comparing the extent of reaction with the equilibrium constant given by the reaction, $CH_4+2CO{\Leftrightarrow}3C+2H_2O$. The simulation showed that catalysts packed in the central region nearer the entrance of the reactor were more prone to coking because of the regional characteristics of lower temperature, lower concentration of $H_2O$, and higher concentration of CO. With the higher feeding temperature, the feed gas composition of the increased $H_2O$ and correspondingly decreased $CO_2$, or the decrease in the reactor diameter, the volume fraction of the catalyst bed subsequent to coking could be diminished. Throughout the simulation, reactor dimension and reaction condition for coking-free operation were suggested.