• KSBB Journal
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• 제18권2호
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• pp.79-89
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• 2003

This article contains an overview of acoustic wave devices, the theory and application of piezoelectric quartz crystal microbalances(PZ QCM), clinical analysis, gas phase detection, DNA biosensors, drug analysis, food microbial analysis and environmental analysis.

• 한국식품과학회지
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• 제31권1호
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• pp.1-6
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• 1999

Salmonella spp.의 신속한 검출을 위하여 발진모듈, 수정결정 진동측정기, 박막형태의 수정결정으로 이루어진 압전류적(piezoelectric, PZ) 항체센서 시스템을 구성하였다. 수정결정의 금전극 표면에 Salmonella 구조 항원(common structural antigen)에 대한 항체를 protein A를 사용하여 고정화하고, 항체가 고정화된 수정결정과 미생물간의 결합반응에 의한 질량증가로 나타나는 진동수의 감소량을 측정하였다. PZ 항체센서는 $35^{\circ}C$, pH 7.2의 0.1M 인산 완충용액에서 Salmonella균에 대하여 가장 높은 감응도를 나타내었다. PZ 항체센서의 반응은 Salmonella균에 대하여 매우 선택적이었고 polystyrene bead 첨가시 센서의 감응도가 크게 증가하였다. Salmonella균의 농도가 $10^5{\sim}10^6\;CFU/mL$의 범위 내에 있을 때 쌍대수좌표에서 직선구간의 검량선을 얻을 수 있었고, Salmonella 검출에 소요되는 시간은 50분이내 이었다.

• Journal of Applied Biological Chemistry
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• 제44권4호
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• pp.163-166
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• 2001

Piezoelectric (PZ) crystal biosensor system was used to detect the DNA of food pathogenic Listeria monocytogenes. L. monocytogenes-specific DNA was multiplied via the polymerase chain reaction using LM1 oligonucleotide (5'-TTACGAATTAAAAAGGAGCG-3') and LM2 oligonucleotide (5'-TTAAATCAGCAGGGGTCTTT-3') as primers. DNA fragment of 161 bp, which was specific only for L. monocytogenes, was observed. To obtain a large amount of single-stranded DNA containing an SH group used for coupling to the gold electrode chemisorptively, LM1 oligonucleotide containing a mercaptohexyl group was utilized as a single strand PCR primer. The PCR product was immobilized onto the gold electrode of PZ crystal, and hybridization was monitored in quartz crystal microbalance (QCM) system by injecting the antisense single-stranded DNA of 161 nucleotides obtained via the single strand PCR using the unmodified LM2 primer. Approximately 70 Hz of frequency drop was observed in the QCM system in the case of two consecutive injections of $5{\mu}g$ of the antisense single-stranded DNA.