Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 31 Issue 1
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- Pages.1-6
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- 1999
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- 0367-6293(pISSN)
Rapid Detection of Salmonella spp. by Antibody Immobilization with Gold-protein A Complex
Gold-protein A Complex 항체 고정화법을 이용한 Salmonella spp.의 신속 검출
- Park, In-Seon (Korea Food Research Institute) ;
- Kim, Nam-Soo (Korea Food Research Institute)
- Published : 1999.02.28
Abstract
A piezoelectric (PZ) biosensor system detecting Salmonella spp. was developed. The system consisted of an oscillator, a frequency counter and an antibody-immobilized quartz crystal. An anti-Salmonella antibody was immobilized on one gold. surface of the quartz crystal with protein A. Salmonella detection was made by measuring resonant frequency shift owing to a mass change by specific binding of microbial cells to the gold surface of the PZ crystal. The PZ antibody sensor was operated optimally at 0.1M phosphate buffer, pH 7.2 and
Salmonella spp.의 신속한 검출을 위하여 발진모듈, 수정결정 진동측정기, 박막형태의 수정결정으로 이루어진 압전류적(piezoelectric, PZ) 항체센서 시스템을 구성하였다. 수정결정의 금전극 표면에 Salmonella 구조 항원(common structural antigen)에 대한 항체를 protein A를 사용하여 고정화하고, 항체가 고정화된 수정결정과 미생물간의 결합반응에 의한 질량증가로 나타나는 진동수의 감소량을 측정하였다. PZ 항체센서는