• Journal of fish pathology
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• v.20 no.1
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• pp.41-47
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• 2007

To investigate the cause of the mass mortality during rockbream, Oplegnathus fasciatus seed production, the water quality and bacterial counts of sea water in breeding tanks was measured 20days post the hatch. During breeding of rockbream fry, the environmental factors of water quality were detected as pH, ammonia COD, phosphate at the supply of the food organisms and the seawater. pH was decreased from the 8.21 of the 1 day per hatch (dph) to 7.56 of the on the 7 dph. Ammonia was conversely increased 0.49 ppm of the 1 dph to 0.85 ppm of 10 dph. As the adding of the chlorella and the rotifer tanks, COD was increased the 3.3 times and 1.2 times than those of pre-adding respectively. The phosphate and the ammonia were also increased 1.7 and 2.3 times, with adding the chlorella respectively, which exceeded the second grade for sea water evaluation level, 0.015 ppm and 0.1 ppm respectively. Water quality was not improved by PSB (Photosynthetic Bacteria) treatment, which increased the value of COD in 1.7 times, phosphate in 2.7 times and ammonia in 1.4 times. The number of the bacteria was also increased along the dph. According to the treatment of chlorella, the number of total bacteria increased in 1.4 times and those of Vibrio sp. 1.6 times. The lethal concentration of ammonia was investigated that over than 10 ppm could killed the fry of rockbream within 28 hrs, but 40% in 2 ppm.

• Korean Journal of Microbiology
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• v.41 no.1
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• pp.67-73
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• 2005

Photoautotrophic bacteria are promising candidates for the production of poly(3-hydroxybutyrate) (PHB) since they can address the critical problem of substrate costs. In this study, we isolated 25 Tn5-inserted mutants of the Synechocystis sp. PCC 6803 which showed enhanced PHB accumulation compared to the wild-type strain. After 5-days cultivation under nitrogen-limited mixotrophic conditions, the intracellular levels of PHB content in these mutants reached up to $10-30\%$ of dry cell weight (DCW) comparable to $4\%$ of DCW in the wild-type strain. Using the method of inverse PCR, the affected genes of the mutants were mapped on the completely known genome sequence of Synechocystis sp. PCC 6803. As a result, the increased PHB accumulation in 5 mutants were found to be resulted from defects of genes coding for NADH-ubiquinone oxidoreductase, O-succinylbenzoic-CoA ligase, photosystem II PsbT protein or histidine kinase, which are involved in photosystem in thylakoid inner membrane of the cell. The values of $NAD(P)H/NAD(P)^+$ ratio in the cells of these mutants were much higher than that of the wild-type strain as measured by using pulse-amplitude modulated fluorometer, suggesting that PHB synthesis could be enhanced by increasing the level of cellular NAD(P)H which is a limiting substrate for NADPH-dependent acetoacetyl-CoA reductase. From these results, it is likely that NAD(P)H would be a limiting factor for PHB synthesis in Synechocystis sp. PCC 6803.

• Tuberculosis and Respiratory Diseases
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• v.49 no.2
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• pp.198-206
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• 2000

Background : Pulmonary complications following bonemarrow transplantation (BMT) are common and associated with a high mortality rate. We investigated the yield, safety, and impact of fiberoptic bronchoscopy (FOB) for diagnosis of postBMT pneumoniae. Methods : From May 1997 to April 2000, 56 FOBs were performed in 52 post BMT patients for clinical pneumoniae. BMT patients with respiratory symptoms and/or pulmonary infiltrates had a thoracic HRCT(high resolution computed tomography) and bronchoscopic examination including BAL (bronchoalveolar lavage), TBLB (transbronchial lung biopsy), PSB (protected specimen brush). Results : The characteristics of the subjects were as follows : 37 males, 15 females, mean age of 31.3 years(l7-45), 35 sibling donor allogenic BMTs, 15 nonrelated donor allogenic BMTs, and 2 autologous BMTs. Fiftynine percent of FOBs (33 FOBs, 31 patients) were diagnostic. Isolated pathogens included the following : 12 cytomegalovirus (CMV) (21.4 %), 7 pneumocystis carinii (PC) (12.5 %), 11 CMV with PC (19.6 %), 2 Mycobacaterium tuberculosis (3.6%), and 1 streptococcus (1.8%). Most of the radiographic findings were diffuse interstitial lesions. CMV pneumoniae had mainly diffuse interstitial nodular lesion, and PC pneumoniae had diffuse, interstitial ground glass opacity(GGO). When CMV was accompanied by PC, a combined pattern of nodular and GGO was present. Of the 56 cases (23.2%), 13 died of CMV pneumoniae (n=2), PCP (n=2),mixed infection with CMV and PC (n=3), underlying GVHD (n=1), underlying leukemia progression (n=1), or respiratory failure of unknown origin (n=4). There was no major complication by bronchoscopy. Only 3 cases developed minor bleeding and 1 episode temporary hypoxemia. Conclusion : Based on our findings, CMV and PC are the major causes of postBMT pneumoniae. In addition, BAL can be considered a safe and accurate procedure for the evaluation of pulmonary complications after BMT.

• Korean journal of applied entomology
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• v.25 no.1 s.66
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• pp.41-46
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• 1986

Exogenous factor and nutrients affecting for conidial germination of Colletetrichum dematium f. sp. capsicum causing red pepper anthracnose were studied by slide germination test. Optimum temperature of conidial germination was at $28^{\circ}C$, ranging 15 to $35^{\circ}C$. Optimum pH was at 5.5, ranging 4.5 to 8.0, and more than 90% of relative humidity (RH) was optimum. Poor conidial germination of the fungus was observed on sterile distilled water, but potato sucrose broth (PSB), red pepper fruit broth (RPFB), green pepper fruit broth (GPFB) and pepper leaf broth (PLB) furnished a satisfactory nutrients for conidial germination. Exogenous supply of carbon and nitrogen sources were essential for conidial germination, while potassium, phosphorous and sulfur were not evident as that for carbon and nitrogen. Soluble starch was the most suitable as a carbon source for conidial germination and followed by D-glucose, D-galactose and lactose in that order. Maximum germination was attained in the $1{\times}10^4$ conidia per ml. Germination was decreased with increment of conidial concentration, and in the density of $5{\times}10^4$ conida per ml, germination was nearly supipressed. It suggested existing a self-inhibitor. Non-washed conidia germinated more than washed conidia, and conidial germination was also gradually decreased by increasing conidial density.

• Journal of Mushroom
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• v.17 no.2
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• pp.64-69
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• 2019

The present study was conducted to investigate the synergistic effects caused by single and co-inoculation of the phosphate solubilizing bacteria (PSB), Paraburkholderia phenazinium YH3 and Paraburkholderia metrosideri YH4. Phosphate solubilization was assessed by measuring the phosphorus contents for 7 days in a single and co-inoculation medium. Co-inoculation of the two strains was found to release the highest content of soluble phosphorus ($1,250{\mu}g\;mL^{-1}$) into the medium, followed by the single inoculation of P. metrosideri YH4 ($1196.59{\mu}g\;mL^{-1}$) and P. phenazinium YH3 ($994.34{\mu}g\;mL^{-1}$). The highest pH reduction, organic acid production and glucose consumption was also observed in the co-inoculation medium of the two strains. A plant growth promotion bioassay revealed that co-inoculation with the two strains enhanced the growth of romaine lettuce more than single inoculation with either of the two strains (28.5% for leaf and 16.6% for root). Although there was no significant difference between single and co-inoculation of bacterial strains in terms of phosphorous release and plant growth, the synergistic effects of co-inoculation with PSB could be beneficial for crop growth.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.365-376
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• 2020

Immature embryogenesis is a useful process in wheat tissue culture, including transgenic technology, because of its high regeneration efficiency compared to that in other tissues. However, it is a very labor-intensive and time-restrictive method, because the preparation of immature embryos is limited to the optimal time after flowering. In this experiment, 'Speed Breeding', a breeding technique that accelerates breeding generation advancement by extending the photoperiod, was applied to the wheat variety 'Bobwhite'. A controlled growth room was constructed by adjusting the photoperiod (22-hour light/2-hour dark) using LED lights at temperature of 22℃. After vernalization of the Bobwhite seeds at 4℃ for 4 weeks, the seedlings were grown in a controlled growth room and a greenhouse to compare the heading date. In both conditions, calli were induced from immature embryos on the 11^th day after flowering. After 4 weeks, the calli were transferred to a regeneration medium. Regeneration efficiencies under greenhouse conditions and Speed Breeding conditions were determined as 45.05% and 43.18%, respectively. Antioxidant enzyme activity and reference gene expression analysis were performed to confirm the presence of stress due to an extremely long-day photoperiod. As a result, the antioxidant enzyme activity was not distinguished from that of the greenhouse condition. The reference gene expression analysis revealed that the PsaA and CDC genes were highly expressed under the Speed Breeding condition. However, expression of PsbA was similar expression in both conditions. These results will provide useful information for the application of immature embryogenesis to the wheat transformation system.

• Korean journal of communication and information
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• v.52
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• pp.69-89
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• 2010

We investigated the frames represented in the news media coverage in Korean daily newspapers during dismissal procedure of the director-general of the public service broadcaster, KBS. We analyzed 470 newspaper stories in the period surrounding the dismissal. The use of news frames in newspapers depended on the disposition of outlets, conservative or progressive. Conservative newspapers, ChoSun and DongA, described the dismissal of the director -general with a generally favorable or supportive view, but progressive outlets, Hankyoreh and KyeongHyang, reported more and non-favorable for the issue. Our results showed that conservative newspapers more often used episodic and cause for dismissal frame in the presentation of news, whereas progressive more often used thematic and media independence frames. Main frames commonly used in the news are the cause for dismissal, conflict, media independence, and regime maintenance, respectively, which are formed by 9 minor news frames. Conservative newspapers concentered on the cause attribution; management responsibility, conflicts, and dismissal procedures, but progressive focused on media independence and dismissal procedures. All newspapers selectively quoted groups, institutions and actors to enhance their messages and frames. Finally, there was significant difference between stories in each newspapers and the court's ruling for the dismissal.

• Korean Journal of Environmental Agriculture
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• v.17 no.3
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• pp.279-285
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• 1998

Fenitrothion-degrading microorganisms were isolated from 124 sampling sites of paddy, upland, forest and polluted soil, and wastewater. A total of 1,071 strains were isolated from each selective medium supplemented with 50mg/l of fenitrothion - nutrient agar (NA) 601, potato dextrose agar (PDA) 201, Actinomycetes isolation agar (AIA) 168 and basal salt medium (BSM) 101, respectively. Twenty-eight effective strains of them, which showed more than 80% degradation of fenitrothion by the gasliquid chromatography(GLC) analysis. were successfully selected from each liquid culture supplemented with 50mg/l of fenitrothion - NB 12(upland soil 3, paddy soil 3, forest soil 2, polluted soil 4), PDB 8(upland soil 1, paddy soil 2, forest soil 2, polluted soil 3) and PSB 8(upland soil 1, forest soil 1, polluted soil 6), respectively. Four strains - NPal, NFol, PFol and BPol, which have the most powerful degradation activity were finally selected among 28 fenitrothion-degrading microorganisms based on the degradation rate at the concentration of 100mg/l fenitrothion in enrichment media.

• Proceedings of the Korean Information Science Society Conference
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• 2005.07a
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• pp.709-711
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• 2005

컴퓨터 그래픽스 분야의 발전으로 3D 모델의 수가 기하급수적으로 늘고 있다. 기존의 텍스트나 2D 이미지만을 검색하는 시스템으로는 정확한 3D 모델의 검색이 힘들다. 따라서 3D 모델 검색 시스템의 필요성이 대두되고 많은 분야에서 그 정확도와 속도향상을 위한 3D 모델 검색 연산자(Descriptor)와 검색 알고리즘을 개발하기 위한 연구가 진행 중이다. 본 논문에서는 VRML 모델을 XML 데이터로 변환하여 3D 모델 검색에 사용하는 것이 주요 목표이다. 검색 방법은 크게 VRML의 노드 분류화를 통한 기본 도형에 대한 검색과 XML로 변환하면서 생성하는 무게중심(Mass-Center)을 이용한 검색 두 가지이다. 즉, 3D 모델 데이터베이스를 구축함으로써 VRML 노드를 통한 분류화와 라벨화된 3D 모델 데이터베이스 지원 등의 장점을 활용한다. 3D 모델을 Key값(Descriptor)을 생성하여 분류화된 XML 데이터로 저장하고, 처리하여 유사도 비교의 대상과 횟수가 많아질수록, 3D 모델을 바로 데이터베이스에서 검색에 사용할 수 있어 검색의 속도와 성능을 보다 증가시킬 수 있다. 보다 복잡한 3D 모델의 유사도 비교에 있어서는 Princeton Shape Benchmark(PSB)[1]에서 정확도가 가장 높게 평가된 방법인 LFD(Light Field Descriptor)[6] 검색 연산자를 사용한다. 이 방법은 3D 모델에서 2D 이미지를 얻어 검색하는 방법으로 많은 2D 이미지 관측점(View-Point)과 관측된 2D 이미지의 적합도를 비교하는 계산량이 많은 단점이 있다. 그래서 3D 모델 검색을 위한 2D 이미지 관측에 있어 x, y, z축 방향의 관측점을 얻는 방법을 제안함으로써 2D 이미지의 관측점을 줄여 계산량을 대폭 감소시키는 장점을 갖는다.것으로 조사되었으며 40대 이상의 연령층은 점심비용으로 더 많은 지출을 하고 있는 것으로 나타났다. 4) 끼니별 한식에 대한 선호도는 아침식사의 경우가 가장 높았으며, 이는 40대와 50대에서 높게 나타났다. 점심 식사로 가장 선호되는 음식은 중식, 일식이었으며 저녁 식사에서 가장 선호되는 메뉴는 전 연령층에서 일식, 분식류 이었으며, 한식에 대한 선택 정도는 전 연령층에서 매우 낮게 나타났다. 5) 각 연령층에서 선호하는 한식에 대한 조사에서는 된장찌개가 전 연령층에서 가장 높은 선호도를 나타내었고, 김치는 40대 이상의 선호도가 30대보다 높게 나타났으며, 흥미롭게도 30세 이하의 선호도는 30대보다 높게 나타났다. 그 외에도 떡과 죽에 대한 선호도는 전 연령층에서 낮게 조사되었다. 장아찌류의 선호도는 전 연령대에서 낮았으며 특히 30세 이하에서 매우 낮게 조사되었다. 한식의 맛에 대한 만족도 조사에서는 연령이 올라갈수록 한식의 맛에 대한 만족도는 낮아지고 있었으나, 한식의 맛에 대한 만족도가 높을수록 양과 가격에 대한 만족도는 높은 경향을 나타내었다. 전반적으로 한식에 대한 선호도는 식사 때와 식사 목적에 따라 연령대 별로 다르게 나타나고 있으나, 선호도는 성별이나 세대에 관계없이 폭 넓은 선호도를 반영하고 있으며, 이는 대학생들을 대상으로 하는 연구 등에서도 나타난바 같다. 주 5일 근무제의 확산과 초 중 고생들의 토요일 휴무와 더불어 여행과 엔터테인먼트산업은 더욱 더 발전을 거듭하고 있으며, 외식은 여행과 여가 활동의 필수적인 요소로써 그 역할을 일조하고 있다. 이와 같은 여가시간의 증가는 독신자들에게는 좀더 많은 여유시간을 가족을 이루고 있는 가족구성원들에게는 가족과의 유대를 강화하는 휴식과 오락의 소비 트렌드를 창출시켰다. 이와 더불어 외식은 식사를 해결하기 위한

• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.390-394
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• 2004

The genetically modified glyphosate-tolerant soybean contains the following introduced DNA sequences: the EPSPS (5-enol-pyruvylshikimate-3-phosphate synthase) gene from Agrobacterium sp. strain CP4, the 35S promoter from the cauliflower mosaic virus, and the NOS terminator from Agrobacterium tumefaciens. In the present study, detection of these introduced DNAs was performed by amplification using the polymerase chain reaction (PCR). A multiplex PCR method was also applied to prevent false positive results. When primers for 35S promoter, nos3', CTP(chloroplast transit peptide), and CP4 EPSPS (EPSPS from Agrobacterium sp. CP4) were used, positive results were obtained in PCR reactions using DNA from genetically modified glyphosate-tolerant soybeans. There were no false positive results when using DNA from non-genetically modified soybeans. The CP4 EPSPS gene was detected when less than 125 pg glyphosate-tolerant soybean DNA was amplified. Lectin Lel and psb A were amplified from both non-genetically modified and genetically modified glyphosate-tolerant soybean DNA. Multiplex PCR was performed using different primer sets for actin Sacl, 35S promoter and CP4 EPSPS. The actin gene was detectable in both non-genetically modified and glyphosate-tolerant soybeans as a constant endogenous gene. Target DNAs for the 35S promoter, and CP4 EPSPS were detected in samples containing 0.01-0.1% glyphosate-tolerant soybean, although there were variations depending on primers by multiplex PCR. Soybean seeds from five plants of non-genetically modified soybean were co-cultivated for six months with those of genetically modified soybean, and they were analyzed by PCR. As a result, they were not positive for 35S promoter, nos3' or CP4 EPSPS. Therefore, these results suggest there was no natural crossing of genes between glyphosate-tolerant and non-genetically modified soybean during co-cultivation, which indicates that gene transfer between these plants is unlikely to occur in nature.