• ALGAE
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• v.34 no.2
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• pp.71-90
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• 2019

A new encrusting red alga was found growing abundantly on glass debris items that drifted ashore along the coasts of Oregon and Washington. These included discarded fluorescent tubes, incandescent light bulbs, capped liquor bottles, and ball-shaped fishing-net floats. Field collections and unialgal cultures of the alga revealed that it consisted of two morphological phases: a young loosely aggregated turf and a mature consolidated mucilaginous crust. The turf phase consisted of a basal layer of globose cells that produced erect, rarely branched, uniseriate to multiseriate filaments up to $500{\mu}m$ long with closely spaced cells lacking pit-plugs. These filaments expanded in size from their bases to their tips and released single cells as spores. At maturity, a second phase of growth occurred that produced a consolidated crust, up to $370{\mu}m$ thick. It consisted of a basal layer of small, tightly appressed ellipsoidal-to-elongate cells that generated a mucilaginous perithallial matrix containing a second type of filament with irregularly spaced cells often undergoing binary division. At the matrix surface, the original filaments continued to grow and release spores but often also eroded. Individual cells, examined using confocal microscopy and SYBR Green staining, were found to contain a central nucleus, a single highly lobed peripheral chloroplast without a pyrenoid, and numerous chloroplast nucleoids. Morphological data from field and culture isolates and molecular data (rbcL, psbA, and SSU) show that this alga is a new genus and species which we name Viator vitreocola, "a traveller on glass."

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.865-873
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• 2018

Allium ochotense and Allium microdictyon are commonly known as 'Mountain garlic' and are popular, economically important species in many countries such as Korea, China, and Mongolia. Their leaves are used as culinary side dishes and in traditional medicines. In Korea, these two species are at risk of extinction due to damage to their natural habitat and thus, conservation and breeding programs are needed. However, their identification relies mostly on morphological data, which is limited and until recently, led to classifying these two species under A. victorialis. In the present study, a simple and reliable method of molecular identification was developed to distinguish A. ochotense from A. microdictyon that targets four barcoding regions: the internal transcribed spacer (ITS), the maturase K gene (matK), the chloroplast psbA-trnH intergenic region, and the ribulose-bisphosphate carboxylase large subunit gene (rbcL). Single nucleotide polymorphisms (SNPs) were found in ITS and matK regions, and species-specific primers were designed based solely on the SNP at position 680 of the ITS region that could differentiate A. ochotense from A. microdictyon. Using these primers in amplification refractory mutation system (ARMS)-PCR, A. ochotense, and A. microdictyon could be simultaneously and efficiently distinguished. This study is the first to report a simple, rapid, and efficient method for discriminating A. ochotense and A. microdictyon, indicating the utility of species-specific markers in the development of conservation and breeding programs.

• Korean Journal of Agricultural Science
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• v.49 no.1
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• pp.1-10
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• 2022

One hundred and twenty imprinting control region (ICR) mouse with initial body weights of 26 ± 2 g (5 weeks old) were assigned to six treatments for a two-week feeding trial to determine the effect of Pediococcus pentosaceus strains (PpS) which were isolated from three different types of Kimchi in ICR mice infected with Escherichia coli (Ec) or Salmonella Typhimurium (ST). Six groups constituted a normal control group without Ec or ST orally administrated (NC-; n = 20), a normal control group (NC+; n = 20), a group for which Lactobacillus plantarum was orally administrated (LP; n = 20), a group for which PpS A was orally administrated (PSA; n = 20), a group for which PpS B was orally administrated (PSB; n = 20), and a group for which PpS C was orally administrated (PSC; n = 20), the latter five groups constituted the Ec infected groups and the ST infected groups of 10 mice each. LP and PSC showed significantly (p < 0.05) improved growth performance compared to the other groups, except for NC- in the Ec infected mice group. NC+ showed significantly lower (p < 0.05) growth performance compared to the other groups, except for NC- in the ST infected mice groups. Regarding the Ec and Salmonella counts in the intestine, the LP and PSC groups had significantly lower (p < 0.05) counts than the NC+ and PSB groups. In conclusion, LP and PSC strains isolated from Kimchi can act as probiotics by inhibiting Ec and ST.

• Journal of Mushroom
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• v.15 no.4
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• pp.183-189
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• 2017

This study investigated the synergistic effect of single inoculation and co-inoculation of phosphate-solubilizing bacteria (PSB) Burkholderia metallica JH-7 and Burkholderia contaminans JH-15. Phosphate-solubilizing abilities of these strains were assessed by measuring phosphorus content in culture media that were singly inoculated or co-inoculated with these strains for 7 days. B. metallica JH-7 was found to release the highest content of soluble phosphorus ($140.80{\mu}g\;mL^{-1}$ ) into the medium, followed by single inoculation of B. contaminans JH-15 ($135.95{\mu}g\;mL^{-1}$ ) and co-inoculation of two strains ($134.84{\mu}g\;mL^{-1}$ ). The highest pH reduction, organic acid production, and glucose consumption were observed in the medium inoculated with B. metallica JH-7 alone compared with that in the medium co-inoculated with both the strains. Results of a plant growth promotion bioassay showed 17.4% and 7.48% higher leaf and root growth, respectively, in romaine lettuce inoculated with B. metallica JH-7 alone than in romaine lettuce inoculated with a control strain. However, no significant difference was observed between single inoculation and co-inoculation of these strains with respect to phosphorus release and plant growth. Although the results of the present study did not show the synergistic effect of phosphate solubilization by the PSB strains examined, these results indicate that treatment with PSB exerts a beneficial effect on crop growth.

• Journal of Food Hygiene and Safety
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• v.27 no.4
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• pp.466-472
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• 2012

In this study, ribulose bisphosphate carboxylase (rbcL), RNApolymeraseC (rpoC1), intergenic spacer (psbA-trnH), and second internal transcribed spacer (ITS2) as identification markers for discrimination of P. mirifica in foods were selected. To be primer design, we obtained 719 bp, 520 bp, 348 bp, and 507 bp amplicon using universal primers from selected regions of P. mirifica. The regions of rbcL, rpoC1, and psbA-trnH were not proper for design primers because of high homology about P. mirifica, P. lobata, and B. superba. But, we had designed 4 pairs of oligonucleotide primers from ITS2 gene. Predicted amplicon from P. mirifica were obtained 137 bp and 216 bp using finally designed primers SFI12-miri-6F/SFI12-miri-7R and SFI12-miri-6F/SFI12-miri-8R, respectively. The species-specific primers distinguished P. mirifica from related species were able to apply food materials and processed foods. The developed PCR method would be applicable to food safety management for illegally distributed products in markets and internet shopping malls.

• Nuclear Engineering and Technology
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• v.10 no.4
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• pp.203-213
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• 1978

A combination of computer codes such as LATREP, HWR, AXAV and CITATION is utilized in an attempt to analyze the nuclear design characteristics of the CANDU-PHWR of the Wolsung Unit 1. The major nuclear properties to be computed are tile lattice properties of CANDU fuel channel and the core channel power distribution. The computed results are compared with the PSR documentation for the Wolsung reactor. The observed discrepancies between our computation and the PSB values are discussed in terms of incomplete information on the description of the core configuration in the PSR and the different calculation methods.

• Journal of Power Electronics
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• v.8 no.3
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• pp.259-267
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• 2008

Power quality improvement in a three-phase four-wire system is achieved using a new topology of DSTATCOM (distribution static compensator) consisting of a star/delta transformer with a tertiary winding and a three-leg VSC (voltage source converter). This new topology of DSTATCOM is proposed for power factor correction or voltage regulation along with harmonic elimination, load balancing and neutral current compensation. A tertiary winding is introduced in each phase for a delta connected secondary in addition to the star-star windings and this delta connected winding is responsible for neutral current compensation. The dynamic performance of the proposed DSTATCOM system is demonstrated using MATLAB with its Simulink and Power System Blockset (PSB) toolboxes under varying loads. The capacitor supported DC bus of the DSTATCOM is regulated to the reference voltage under varying loads.

• Journal of Electrical Engineering and Technology
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• v.2 no.3
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• pp.358-365
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• 2007

This paper deals with a novel solid state controller (NSSC) for parallel operated isolated asynchronous generators (IAG) feeding 3-phase 4-wire loads in constant power applications, such as uncontrolled pico hydro turbines. AC capacitor banks are used to meet the reactive power requirement of asynchronous generators. The proposed NSSC is realized using a set of IGBTs (Insulated gate bipolar junction transistors) based current controlled 4-leg voltage source converter (CC-VSC) and a DC chopper at its DC bus, which keeps the generated voltage and frequency constant in spite of changes in consumer loads. The complete system is modeled in MATLAB along with simulink and PSB (power system block set) toolboxes. The simulated results are presented to demonstrate the capability of isolated generating system consisting of NSSC and parallel operated asynchronous generators driven by uncontrolled pico hydro turbines and feeding 3-phase 4-wire loads.

• Journal of Power Electronics
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• v.8 no.1
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• pp.81-90
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• 2008

This paper presents an investigation of a voltage and frequency controller for an isolated asynchronous generator (IAG) driven. by a wind turbine and supplying 3-phase 4-wire loads to the isolated areas where a grid is not accessible. The control strategy is based on the indirect current control of the VSC (voltage source converter) using the frequency PI controller. The proposed controller consists of three single-phase IGBT (Insulated Gate Bipolar Junction Transistor) based VSC, which are connected to each phase of the IAG through three single phase transformers and a battery at their DC link. The controller has the capability of controlling reactive and active powers to regulate the magnitude and frequency of the generated voltage, harmonic elimination, load balancing and neutral current compensation. The proposed isolated system is modeled and simulated in MATLAB using Simulink and PSB (Power System Block-set) toolboxes to verify the performance of the controller.

• Advances in materials Research
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• v.3 no.2
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• pp.91-103
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• 2014

A study that demonstrates how to investigate the molded part quality and the consistency of injection process based on the rheological concept is proposed. It is important for plastic material whose melt viscosity is variable with respect to the processing condition. The formulations to couple the melt viscosity with injection pressure and fill time are derived first. Taking calculations of the measured pressure and the time by using these formulations, the melt viscosity in injection process can be determined on machine. As the relation between the injection speed and the melt viscosity is constructed, the influences of the setting parameter of injection machine on the molded part quality can be investigated through evaluating the state of the melt viscosity. In addition, a pressure sensor bushing (PSB) designed with a quick installation feature is also provided and validated. The results show that a higher injection speed improves the tensile strength of the molded part but also the consistency of the molded part quality. This work provides an alternative to evaluate the molding quality scientifically.