• BMB Reports
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• 제47권4호
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• pp.184-191
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• 2014

Respiratory syncytial virus (RSV) is the leading cause of respiratory infection in infants and young children. Severe clinical manifestation of RSV infection is a bronchiolitis, which is common in infants under six months of age. Recently, RSV has been recognized as an important cause of respiratory infection in older populations with cardiovascular morbidity or immunocompromised patients. However, neither a vaccine nor an effective antiviral therapy is currently available. Moreover, the interaction between the host immune system and the RSV pathogen during an infection is not well understood. The innate immune system recognizes RSV through multiple mechanisms. The first innate immune RSV detectors are the pattern recognition receptors (PRRs), including toll-like receptors (TLRs), retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), and nucleotide-biding oligomerization domain (NOD)-like receptors (NLRs). The following is a review of studies associated with various PRRs that are responsible for RSV virion recognition and subsequent induction of the antiviral immune response during RSV infection.

• 대한전자공학회논문지TC
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• 제41권5호
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• pp.27-36
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• 2004

최근 인터넷 트래픽의 폭발적인 증가에 따라, 매우 빠른 고속 네트워크 장비에 네트워크프로세서(NP)의 사용이 보편화되고 있다. 이에 따라, 기존의 일반적인 마이크로프로세서를 이용한 네트워크 장비의 성능 한계를 벗어나 향상된 성능을 보이는 라우팅 기능과 패킷처리 기능을 분리하는 분산형 시스템 구조가 이용되고 있다. 본 논문에서는 10기가비트 이더넷 포트를 가지는 10기가비트 에지 스위치 시스템에 적용한 패킷 라우팅 처리와 OAM 처리를 위한 분산형 이더넷 IPC 통신 메커니즘과 10Gbps급 이더넷 데이터를 처리할 수 있는 프레임 방식의 MUX/DEMUX 구조를 설계하고 구현하는 방법을 기술한다. 본 논문에서 제안한 분산형 이더넷 UC 통신 메커니즘 구조는 현재 진행되고 있는 10기가비트 이더넷 인터페이스를 갖는 320Gbps급의 백본용 이더넷 스위치 시스템에도 적용하였다.

• 대한수의학회지
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• 제60권3호
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• pp.109-116
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• 2020

This study aimed to develop a semi-nested polymerase chain reaction assay for the direct detection of Mycoplasma synoviae (M. synoviae) from clinical samples using three newly designed oligonucleotide primers specific to the variable lipoprotein haemagglutinin (vlhA) gene and differentiate M. synoviae field strains based on a nucleotide deletion or the insertion of the proline-rich repeat (PRR) region of the vlhA gene. The developed semi-nested polymerase chain reaction (PCR) assay revealed positive results in 12 out of 100 clinical samples collected from chickens showing lameness and joint swelling. Six positive samples were selected randomly for sequencing, and sequence analysis revealed 96.3-100% nucleotide identities compared to the reference sequences. Phylogenetic analysis showed that sequences of the strains in this study were closely related to WVU1853 (Spain), CK.MS.UDL.PK.2014.2 (Pakistan), and F10-2AS (USA) strains, but they were distinct from the M. synoviae-H vaccine strain sequence. M. synoviae obtained from these samples were identified as types A and C with a length of 38 and 32 amino acids, respectively. These results indicated that the specific and sensitive semi-nested PCR could be a useful diagnostic tool for the direct identification of clinical samples, and the sequence analysis of the partial vlhA gene can be useful for typing M. Synoviae.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제4권5호
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• pp.753-775
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• 2010

Greedy forwarding is a key mechanism of geographic routing using distance as a metric. As greedy forwarding only uses 1-hop neighbor node information, it minimizes routing overhead and is highly scalable. In existing greedy forwarding schemes, a node selects a next forwarding node based only on the distance. However, the signal strength in a realistic environment reduces exponentially depending on the distance, so that by considering only the distance, it may cause a large number of data packet retransmissions. To solve this problem, many greedy forwarding schemes have been proposed. However, they do not consider the unreliable and asymmetric characteristics of wireless links and thus cause the waste of limited battery resources due to the data packet retransmissions. In this paper, we propose a reliable and energy-efficient opportunistic greedy forwarding scheme for unreliable and asymmetric links (GF-UAL). In order to further improve the energy efficiency, GF-UAL opportunistically uses the path that is expected to have the minimum energy consumption among the 1-hop and 2-hop forwarding paths within the radio range. Comprehensive simulation results show that the packet delivery rate and energy efficiency increase up to about 17% and 18%, respectively, compared with the ones in PRR${\times}$Distance greedy forwarding.

• 대한수의학회지
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• 제38권1호
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• pp.77-83
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• 1998

돼지 생식기호흡기증후군(porcine reproductive and respiratory syndrome, PRRS) 바이러스가 웅돈에 감염되었을 경우에는 정충의 기형 등 정액의 질 저하와 더불어 정액에 바이러스가 함유되어 있어 종부시 모돈에 바이러스를 전파하는 것으로 알려져 있다. 감염된 웅돈의 정액으로 인공수정을 실시할 경우 농장의 모돈 전체에 순식간에 바이러스를 전파하여 막대한 피해를 유발할 가능성이 높다. 따라서 감염웅돈을 신속히 검색하여 격리함으로써 피해를 사전에 방지해야 하며, 이를 위해서 웅돈의 감염여부를 신속히 확진하는 진단법의 개발이 필요한 실정이다. PRRS의 진단을 위해서는 바이러스학적인 진단법으로는 바이러스 분리동정, 혈청학적인 방법으로 바이러스 분리동정이 필수적이나 검사시간이 많이 소요되고, 분리동정 자체가 까다로운 단점이 있다. 본 연구에서는 웅돈의 정액내에서 PRRSV 바이러스에 대한 유전자를 RT-PCR법으로 증폭하는 방법을 개발하였으며, 진단의 민감도를 높이기 위하여 Nested PCR법으로 재확인 할 경우, 바이러스의 역가가 $1TCID_{50}$만 함유되어 있어도 진단이 가능한 조건을 확립하였다. 이 방법을 이용할 경우 웅돈의 정액시료에 대한 PRRS 바이러스 감염여부를 신속, 정확하게 검색하여 감염웅돈을 통한 PRRS바이러스의 전파를 미리 차단할 수 있으므로 PRRS방제에 효과적으로 이용될 것으로 사료된다.

• BMB Reports
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• 제50권5호
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• pp.235-236
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• 2017

The circadian clock is an internal system that is synchronized by external stimuli, such as light and temperature, and influences various physiological and developmental processes in living organisms. In the model plant Arabidopsis, transcriptional, translational and post-translational processes are interlocked by feedback loops among morning- and evening-phased genes. In a post-translational loop, plant-specific single-gene encoded GIGANTEA (GI) stabilize the F-box protein ZEITLUPE (ZTL), driving the targeted-proteasomal degradation of TIMING OF CAB EXPRESSION 1 (TOC1) and PSEUDO-RESPONSE REGULATOR 5 (PRR5). Inherent to this, we demonstrate the novel biochemical function of GI as a chaperone and/or co-chaperone of Heat-Shock Protein 90 (HSP90). GI prevents ZTL degradation as a chaperone and facilitates ZTL maturation together with HSP90/HSP70, enhancing ZTL activity in vitro and in planta. GI is known to be involved in a wide range of physiology and development as well as abiotic stress responses in plants, but it could also interact with diverse client proteins to increase protein maturation. Our results provide evidence that GI helps proteostasis of ZTL by acting as a chaperone and a co-chaperone of HSP90 for proper functioning of the Arabidopsis circadian clock.

• 대한의생명과학회지
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• 제25권1호
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• pp.60-65
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• 2019

Toll-like receptors (TLRs) are one of the families of pattern recognition receptors (PRR) operating in the innate immunity. TLRs have the ability to recognize relatively conserved microbial components, which are generally referred to as pathogen-associated molecular patterns (PAMPs). The activation of TLRs signaling leads to the activation of $NF-{\kappa}B$ and the expression of pro-inflammatory gene products such as cytokines and inducible nitric oxide synthase (iNOS). To evaluate the therapeutic potential of pristimerin, which is a naturally occurring triterpenoid compound from Celastraceae plants, iNOS expression induced by MALP-2 (TLR2 and TLR6 agonist), Poly[I:C] (TLR3 agonist), or LPS (TLR4 agonist) were examined. Pristimerin suppressed the iNOS expression induced by MALP-2, Poly[I:C], or LPS. These results suggest that pristimerin can modulate TLRs signaling pathways leading to decreased inflammatory gene expression.

• 대한의생명과학회지
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• 제25권3호
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• pp.267-274
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• 2019

Toll-like receptors (TLRs) are one of the families of pattern recognition receptors (PRR) to recognize pathogen-associated molecular patterns (PAMPs). PAMPs stimulate TLRs to initiate specific immunoactivity. The activation of TLRs signaling leads to the expression of pro-inflammatory gene products such as cytokines and inducible nitric oxide synthase (iNOS). To evaluate the therapeutic potential of dehydrocostus lactone (DHL), which is a natural sesquiterpene lactone derived from various medicinal plants, iNOS expression induced by LPS (TLR4 agonist), MALP-2 (TLR2 and TLR6 agonist), or Poly[I:C] (TLR3 agonist) were examined. DHL suppressed the iNOS expression induced by LPS, MALP-2, or Poly[I:C]. DHL also inhibited nitrite production induced by LPS, MALP-2, or Poly[I:C]. These results suggest that DHL can modulate TLRs signaling pathways resulting in anti-inflammatory effect.

• Communications for Statistical Applications and Methods
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• 제31권2호
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• pp.179-189
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• 2024

In multivariate regression with a high-dimensional response Y ∈ ℝ^r and a relatively low-dimensional predictor X ∈ ℝ^p (where r ≥ 2), the statistical analysis of such data presents significant challenges due to the exponential increase in the number of parameters as the dimension of the response grows. Most existing dimension reduction techniques primarily focus on reducing the dimension of the predictors (X), not the dimension of the response variable (Y). Yoo and Cook (2008) introduced a response dimension reduction method that preserves information about the conditional mean E(Y | X). Building upon this foundational work, Yoo (2018) proposed two semi-parametric methods, principal response reduction (PRR) and principal fitted response reduction (PFRR), then expanded these methods to unstructured principal fitted response reduction (UPFRR) (Yoo, 2019). This paper reviews these four response dimension reduction methodologies mentioned above. In addition, it introduces the implementation of the mbrdr package in R. The mbrdr is a unique tool in the R community, as it is specifically designed for response dimension reduction, setting it apart from existing dimension reduction packages that focus solely on predictors.

• IMMUNE NETWORK
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• 제20권3호
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• pp.21.1-21.10
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• 2020

TLRs are pattern recognition receptors (PRRs) whose cytoplasmic signalling domain is similar to that of IL-1. The extracellular domain of TLRs serve as the binding site of pathogen associated molecular patterns. TLRs are found on both plasma and endosomal membranes and they mainly exert their function by activating genes which lead to production of inflammatory factors. The latest TLR to be discovered, TLR10 is a unique TLR which exhibit anti-inflammatory properties. TLR10 is found on the plasma membrane with other TLRs namely TLR1, TLR2, TLR4, TLR5 and TLR6. Studies have revealed that TLR10 is found on the same gene cluster with TLR1 and TLR6 and is also a coreceptor of TLR2. Up to date, TLR10 is the only TLR which exhibit anti-inflammatory property. Previously, TLR10 was thought to be an "orphan receptor" but much recent studies have identified ligands for TLR10. Currently there is no review article on TLR10 that has been published. In this narrative review, we are going to give an account of TLR10, its functions mainly as an anti-inflammatory PRR and its possible applications as a target in therapeutics.