• 대한수의학회지
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• 제30권1호
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• pp.15-27
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• 1990

Effects of oral administration of electrolyte solutions were studied in experimentally dehydrated adult sheep. By the latin square method five ruminal fistulated sheep were examined and dehydrated by deprivation of feed and water for 72 hours. Tap water, physiological saline, 0.45% NaCl+120 mM/L glucose and 0.9% NaCl+1% propylene glycol solution were orally administrated after dehydration, respectively. Rehydration effect and modification of the rumen function were compared. 1. After 72 hours of deprivation of feed and water, sheep were hypertonic dehydrated and blood acid-base parameters were not significantly changed. And there was marked increase in ruminal pH and decrease in ruminal total volatile fatty acid(VFA) concentration. 2. After the fluids administration the changes in blood acid-base parameters were not significant in all groups. 3. Although glucose fermentation in the rumen was observed, 0.45% NaCl+120 mM/L glucose was more effective in rehydration than physiological saline and tap water. But it was difficult to know the rehydration effect of 0.9% NaCl+1% propylene glycol solution exactly because of excessive increase in plasma osmolality. 4. After refeeding, total concentration and proportions of ruminal volatile fatty acid(VFA) were not significantly different among groups and recovered to normal concentration but not in proportions after 2 days in all groups. 5. In vitro cultured ruminal protozoa were susceptible to the decrease of the pH and osmolality.

• 폴리머
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• 제29권3호
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• pp.300-303
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• 2005

폴리우레탄을 PU1000(poly(propylene glycol), PPG, 분자량 1000 g/mol로 제조된 폴리우레탄)과 PU2000(PPG 분자량 2000 g/mol로 제조된 폴리우레탄)을 이용하여 다층구조와 발포체 다층구조로 제조하였고 동력학 특성과 음향특성을 기준물질인 PU1000과 비교 조사하였다. 다층구조 폴리우레탄의 감쇠 피크는 PU1000에 비해 낮은 온도로 이동한 반면에 발포체를 이용한 다층구조에서의 감쇠 피크는 PU1000에 비해 높은 온도로 이동하였고 감쇠 범위 또한 넓어졌다. 음향특성인 투과손실 측면에서 보면 다층구조의 투과손실은 기준이 되는 PU1000에 비해 100 Hz이하와 600 Hz의 특정 주파수 영역에서 효과적이었고 발포체 다층구조의 경우는 실험 범위인 1000 Hz이하 전영역에서 아주 우수한 투과손실이 얻어졌다

• Journal of Pharmaceutical Investigation
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• 제33권1호
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• pp.21-28
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• 2003

Ketorolac tromethamine(KT) is a nonsteroidal agent with potent analgesic and moderate anti-inflammatory activity. The lipid-water partition coefficient of KT was evaluated and KT gel was formulated as a gel containing different pH, different concentrations of polymer (poloxamer 407, carbopol 941), propylene glycol, ethanol and various enhancers. The resulting KT gels were evaluated with respect to their viscosity, in vitro drug permeation rate through hairless mouse skin and stability. In n-octanol and chloroform, the lipid-water partition coefficient of KT was the highest at pH 4 phosphate buffer. The apparent viscosity of KT gel increased with an increase in gel pH, polymer and enhancer concentration. But the apparent viscosity of KT gel decreased with an increase in ethanol concentration. The permeation rate of KT through hairless mouse skin from gels different pH was maximum at pH 4 which is close to KT $pK_{a}$ 3.54. The permeation rate decreased with an increase in polymer, propylene glycol concentration. But the permeation rate increased with an increase in ethanol. The increase of drug concentration from 1 to 3% induced linear increase in permeation rate. The best enhancer was the combination of $Labrasol^{\circledR},\;Transcutol^{\circledR}$, oleic acid and l-menthol. In the accelerated stability test(25, 40 and $50{\circ}C$), pH 5 gel was most stable and pH 4 gel was most unstable for 90 days.

• Journal of Pharmaceutical Investigation
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• 제29권2호
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• pp.111-115
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• 1999

We have studied the transdermal flux of prostaglandin $E_1$ $(PGE_1)$ from a hydrogel patch through hairless mouse skin, to test the possibility of developing a transdermal delivery system. Karaya gum patch containing $PGE_1$ was prepared by casting method. $PGE_1$ was stable in the patch for 10 weeks. The effect of current application, enhancer (propylene glycol monolaurate : PGML), adhesive and patch thickness on the flux was studied using side-by-side diffusion cell. Passive flux of $PGE_1$ was negligible. Cathodal delivery increased the flux about 20 fold. As the concentrations of PGML increased, flux increased. When 5% PGML was used as the enhancer, maximum flux by cathodal iontophoresis was $55\;{\mu}g/cm^2\;hr$. It increased about 2 folds to $100\;{\mu}g/cm^2\;hr$, when the amount of PGML used was 9%. Large increase in flux and the decrease in time to reach maximum flux were observed when the skin was pretreated with neat PGML (maximum flux obtained was about $200\;{\mu}g/cm^2\;hr$). Use of adhesive decreased the flux significantly. To the contrary of our expectation, increase in current density decreased the flux. These flux data together with the stability data indicate that, though the onset of sufficient delivery occur after 1-2 hours of application, therapeutic amount of $PGE_1$ can be delivered through skin using iontophoresis and penetration enhancer.

• 대한화장품학회지
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• 제30권1호
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• pp.123-128
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• 2004

최근 기능성 화장품의 대두로 새로운 형태의 미백제들이 많이 출시되고 있다. 본 연구에서는 천화분근, 하고초엽, 위령선근을 이용하여 새로운 미백제를 개발하였다. 그러나 이러한 미백제는 용해성이 좋지 않고 빛, 열, 산소에 의하여 변색 및 함량의 변화를 가져온다. 먼저 천화분근, 하고초엽, 위령선근을 10배수의 50％ 에탄올을 넣어 75∼85도에서 6∼8시간 추출한 후 여과, 농축, 건조하였다. 건조된 추출물을 이용하여 티로시나제 효소 활성 억제효과, 마우스의 색소세포를 이용한 Bl6 멜라닌 생성 억제 효과, brown guinea pig의 동물 피부의 미백효과 결과 다른 비교 샘플에 비하여 매우 우수한 결과가 나왔다. 또한 천화분근, 하고초엽, 위령선근을 안정화시키기 위해서 propylene glycol (PG)/hydrogenated lecithin/middle chain triglycerides (MCT)/glycerin/water를 고압균질화기를 이용하여 30∼50 nm인 리포좀을 만들었다. 이러한 리포좀은 기존의 처리되지 않은 추출물에 비하여 빛과 열에 3∼5배 안정성을 보였다. 이러한 실험을 위하여 particle size analyzer, freeze fracture transmission electron microscopy (FF-TEM), chromameter, HPLC의 분석장비를 사용하였다.

• 대한화장품학회지
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• 제32권1호
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• pp.29-33
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• 2006

본 연구는 효소의 활성을 저해하는 주위 환경, 특히 열로부터 효소의 활성을 장기간 유지할 수 있는 효소 안정화 시스템에 대한 것으로, 이 시스템은 poly(${\epsilon}-caprolactone$) (PCL) 마이크로 캡슐로, 파파인 효소를 모델 효소로 하여, poly(propylene glycol) (PPG) 층이 코어 효소층을 둘러싸고 있는 형태로 설계되어 있다. 공촛점 현미경 및 장기 열 안정도 결과를 분석해본 결과, 파파인 효소가 소수성 PPG로 둘러쌓여 있고, 배타적 볼륨 효과(exclusive volume effect)에 의해 안정화되어 있음을 밝힐 수 있었다. 이와 같이 향상된 효소의 열 안정도는 소수성 사슬이 긴 PPG를 사용할수록 증가됨을 알 수 있었으며, 이것은 효소와 PPG 계면 사이에서 PPG 층이 파파인 효소를 효과적인 형태 고정(conformational anchoring)을 통해 안정화한 것임을 알 수 있었다.

• 마이크로전자및패키징학회지
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• 제12권4호통권37호
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• pp.359-363
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• 2005

유기 박막트랜지스터 (OTFT)를 제작하기 위하여 게이트 절연막으로서 PVP 계통의 유기막을 갖는 MIM(metal-insulator-metal)구조의 유기 절연층 소자를 제작하였다. 유기 절연층의 형은 ITO/Glass 기판위에 polyvinyl 계열의 PVP(poly-4-vinylphenol)를 용질로, PGMEA (propylene glycol monomethyl ether acetate)를 용매로 사용하여 co-polymer PVP를 제조하였다. 또한 열경화성 수지인 poly(melamine-co-formaldehyde)를 경화제로 사용하여 cross-linked PVP 절연막을 합성하였다. 유기 절연층의 전기적 특성은 co-polymer PVP 소자에 비해 cross-link 방식으로 제조된 소자에서 약 300 pA의 낮은 누설전류와 상대적으로 낮은 잡음전류의 특성을 나타내었다. 또한 cross-linked PVP 절연막에서 보다 양호한 표면형상 (거칠기)이 관찰되었으며 정전용량 값은 약 0.11${\~}$0.18 nF의 값을 나타내었다.

• BMB Reports
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• 제39권6호
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• pp.656-661
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• 2006

Cisplatin (CDDP) is a widely used anticancer drug, but at high dose, it can produce undesirable side effects such as hepatotoxicity. Because silymrin has been used to treat liver disorders, the protective effect of silymarin on CDDP -induced hepatotoxicity was evaluated in rats. Hepatotoxicity was determined by changes in serum alanine aminotransferase [ALT] and aspartate aminotransferase [AST], nitric oxide [NO] levels, albumin and calcium levels, and superoxide dismutase [SOD], glutathione peroxidase [GSHPx] activities, glutathione content, malondialdehyde [MDA] and nitric oxide [NO] levels in liver tissue of rats. Male albino rats were divided into four groups, 10 rats in each. In the control group, rats were injected i.p. with 0.2 ml of propylene glycol in saline 75/25 (v/v) for 5 consecutive days [Silymarin was dissolved in 0.2 ml of propylene glycol in saline 75/25 v/v]. The second group were injected with CDDP (7.5 mg /kg, I.P.), whereas animals in the third group were i.p. injected with silymarin at a dose of 100 mg/kg/day for 5 consecutive days. The Fourth group received a daily i.p. injection of silymarin (100 mg/kg/day for 5 days) 1 hr before a single i.p. injection of CDDP (7.5 mg/kg). CDDP hepatotoxicity was manifested biochemically by an increase in serum ALT and AST, elevation of MDA and NO in liver tissues as well as a decrease in GSH and the activities of antioxidant enzymes, including SOD, GSHPx in liver tissues. In addition, marked decrease in serum NO, albumin and calcium levels were observed. Serum ALT, AST, liver NO level, MDA was found to decreased in the combination group in comparison with the CDDP group. The activities of SOD, GSHPx, GSH and serum NO were lower in CDDP group than both the control and CDDP pretreated with silymarin groups. The results obtained suggested that silymarin significantly attenuated the hepatotoxicity as an indirect target of CDDP in an animal model of CDDP-induced nephrotoxicity.

• 한국식품과학회지
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• 제19권2호
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• pp.119-124
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• 1987

딸기쥬스와 오렌지쥬스 농축액의 빙점을 ${-15}^{\circ}C$로 강하시킬수 있는 복합cryoprotectants를 선정하고, 이를 쥬스에 첨가하여 액체상태로 $-15^{\circ}C$에서 저장하면서 품질변화를 조사하였다. 딸기쥬스에 적합한 복합 cryprotectants의 조성은 설탕25.5%(w/w), 포도당 12.7%, glycerol 1%, propylene glycol 1% 및 ascorbic acid 0.1%였다. 그리고 48% 고형분을 함유하는 농축 오렌지쥬스에 적합한 것으로는 포도당 5%, 과당 5%, glycerol 4% 및 citricacid 1%였다. 저장중 과실쥬스의 종합적 품질은 복합 cryprotectants를 첨가한 것과 대조구사이에 차이가 없었고, 4개월 저장한 딸기쥬스로만들은쨈의 품질에 있어서도 대조구와 처리구간에 유의성 있는 품질차이가 없었다. 이들 결과로 보아 과실쥬스에 복합 cryoprotectants를 첨가하여 냉통온도에서 액체상태로 저장하면 보통 방법으로 냉동저장한것과 대등한 품질을 보존할수 있는 것으로 밝혀졌다.

• Journal of Pharmaceutical Investigation
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• 제29권4호
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• pp.329-335
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• 1999

Clenbuterol, a selective ${\beta}_2-adrenergic$ receptor stimulant, has been introduced as a potent bronchodilator for patients with bronchial asthma, chronic bronchitis and pulmonary emphysema. For the purpose of developing a transdermal preparation for clenbuterol, we attempted to select an optimal solvent system and permeation enhancer among fatty acids and fatty alcohols which are known to accelerate the penetration of various drugs in permeation experiments using hairless mouse skin and Franz diffusion cell. Apparent partition coefficient of clenbuterol was increased as pH of buffer solution was increased and solubility of clenbuterol was increased as the percent of propylene glycol(PG) in buffer solution(pH 10) was increased. Permeability of clenbuterol from different buffer(pH 10)/PG solvent mixtures was decreased as the percent of PG in pH 10 buffer solution was increased and among the various enhancers studied, lauryl alcohol was found to be the most effective enhancer, increasing the permeability of clenbuterol approximately 76-fold compared with control. Lauryl alcohol$(0{\sim}2%)$ enhanced the permeability of clenbuterol concentration-dependently. In this study, the optimal solvent system for the penetration of clenbuterol was found to be 50/50 buffer(pH 10)/PG solvent mixture containing 2% lauryl alcohol.