• 한국수산과학회지
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• 제36권3호
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• pp.230-238
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• 2003

Biosurfactant-producing bacteria, showing strong crude oil degrading activity, were isolated from the caverns of National Oil Storage Basement. From the results of biochemical and molecular biological tests, the isolate was identified as Pseudomonas fluorescens PD101. It grows well on liquid media at temperature range from $20^{\circ}C\;to\;37^{\circ}C,$ but it does not produce biosurfactant when grown at $37^{\circ}C$ or at higher temperature. The biosurfactant was stable at broad pH range from 5 to 11 and under heat treatment condition of $100^{\circ}C$ for 30 min. The biosurfactant produced dark blue halo around the colony when grown on SW agar plates, which could confirm the biosurfactant as one of rhamnolipid group. The 700 bp of PCR product could be amplified from DNA of P. flurorescens PD101 by using PCR primers designed from rh1A gene of P. aeruginosa, and it showed $99\%$ of sequence homology with rh1A gene of P. aeruginosa encoding rhamnosyltransferase 1.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1327-1338
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• 2013

The humanized anti-hepatocyte growth factor (HGF) monoclonal antibody (mAb) YYB-101 is a promising therapeutic candidate for treating various cancers. In this study, we developed a bioprocess for large-scale production of YYB-101 and evaluated its therapeutic potential for tumor treatment using a xenograft mouse model. By screening diverse chemically defined basal media formulations and by assessing the effects of various feed supplements and feeding schedules on cell growth and antibody production, we established an optimal medium and feeding method to produce 757 mg/l of YYB-101 in flask cultures, representing a 7.5-fold increase in titer compared with that obtained under non-optimized conditions. The optimal dissolved oxygen concentration for antibody production was 70% $pO_2$. A pH shift from 7.2 to 7.0, rather than controlled pH of either 7.0 or 7.2, resulted in productivity improvement in 5 L and 200 L bioreactors, yielding 737 and 830 mg/ml of YYB-101, respectively. The YYB-101 mAb highly purified by affinity chromatography using a Protein A column and two-step ion exchange chromatography effectively neutralized HGF in a cell-based assay and showed potent tumor suppression activity in a mouse xenograft model established with human glioblastoma cells.

• 한국미생물·생명공학회지
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• 제20권3호
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• pp.271-279
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• 1992

내알카리성 및 내열성 xylanase를 생산하는 토양분리균인 Bacillus stearothermophilus의 chromosomal DNA와 pBR322 plasmid DNA의 HindIII 절단 DNA 단편을 ligation 시켜 E.coli HB101들 형질전환, 약 5천개의 형질전환체를 얻었으며 이들 중에서 세개의 xylanase 양성 형질전환체를 분리하였다. 상기 세 xylanase 양성 형질전환체들로부터 분리한 제조합 plasmid(pMG11, pMG12 및 pMG13)는 다 같이 xylanase 활성과 관계되는 B.stearothermophilus 유래의 동일 4kb 외래 DNA를 가지고 있었으나, pMG13은 외래 DNA의 삽입 방향만이 다름을 확인하였다.

• 한국컴퓨터그래픽스학회논문지
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• 제24권3호
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• pp.101-109
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• 2018

최근 가상 현실 및 증강 현실 기술의 발달로 다양한 형태의 기술이 제안되고 발전되고 있다. 특히 가상의 학습공간이나 가상의 건축 공간들을 위한 기술로서 원하는 형태의 그림을 그릴 수 있는 가상 드로잉 툴은 필수적인 요소 중 하나로 볼 수 있다. 기존의 래스터 기반 드로잉 알고리즘은 공간 이동이 잦은 가상 현실에서는 부적합 할 수 있는데, 가상 현실의 특성상 근접하거나 멀어지는 경우가 많기 때문이다. 본 논문에서는 연필 및 에어브러시의 물리적인 특성을 고려하여 실제 결과와 흡사하면서도 확대 및 축소에 강건한 가상의 드로잉 툴 렌더링 알고리즘을 제안한다. 캔버스로부터의 거리, 분사되는 잉크의 양, 움직이는 속도, 마찰력, 압력 등을 고려한다. 드로잉의 기본 단위는 정사각형 패치이며 각 패치는 슈도 랜덤값을 기반으로 하는 입자 형태의 이미지를 가진다. 이러한 입자 패치는 슈도 랜덤 식을 사용하고 매 프레임 마다 재 생성되기 때문에 확대 축소 시 깨짐 현상이 발생하지 않는다. 제안된 알고리즘은 2차원 환경 및 가상 현실 환경에 구현되었다. 2차원 환경은 안드로이드 기반 스마트폰에서 구현하였으며 가상 현실 환경은 언리얼 엔진 4를 사용하여 구현하였다. 구현 결과 확대 축소 시에도 이질감 없이 원래의 질감을 유지하는 렌더링 결과를 얻을 수 있었다.

• 한국식품영양과학회지
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• 제38권11호
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• pp.1612-1617
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• 2009

단백질 식품의 가공에 이용할 수 있는 내염성 단백분해효소를 생산하기 위하여, 된장에서 분리한 Aspergillus sp. 101균으로부터 내염성 단백분해효소생산을 위한 최적 배지조성을 확립하였다. 질소원으로서 대두단백원인 탈지대두분(DSF)과 분리대두단백(SPI)을 각각 2% 첨가 시에 효소 활성이 가장 높았고, 무기질소원으로 $CaCO_3$와 $K_2HPO_4$이 각각 0.1%씩 첨가 시에 가장 좋은 결과를 얻었다. 또한 Arabic gum을 배양액에 0.1% 첨가하였을 때, 효소 활성이 가장 높았다. Aspergillus sp. 101은 최고 15% NaCl 농도의 agar plate에서 생육이 가능하였으며, 조효소 또한 NaCl 7%까지 안정하였다. 이와 같이 조정 배지(modified medium)에 Aspergillus sp. 101에 의해 생산된 내염성 단백분해효소는 일반적인 곰팡이류의 단백분해효소 생산용 배지에 비해 생산성이 높았으며, 최적배지에서 배양한 Aspergillus sp. 101 유래 단백분해효소에 의한 어육단백질 가수분해물의 기능 개선 또는 식품첨가물로서의 활용은 단백분해효소 생산의 산업화를 가능하게 하는 것으로 보인다.

• 한국미생물·생명공학회지
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• 제17권2호
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• pp.154-159
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• 1989

Chromosomal DNA fragments of Bacillus sp. YA-14 isolated from soil as a potent xylan hydrolyzing bacterium, were ligated to a vector plasmid, pBR322, and used to transfer Escherichia coli HB101 cells. The recombinant plasmid pYDC21 was found to enable the transformants to produce xylanase. pYDC21 was found to contain the 3 kb HindIII fragment originated from the Bacillus sp. YA-14 chromosomal DNA by southern hybridization. The optimum temperature and pH for the reaction of xylanse produced by E. coli (pYDC21) were appeared at 50$_7$C and pH 7.0, respectiveiy. the xylanase enzyme was stable between pH 5.0 and 7.0 and maintained stably up to 4$0^{\circ}C$.

• 대한임베디드공학회논문지
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• 제9권2호
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• pp.101-107
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• 2014

Mobile robots use an environment map of its workspace to complete the surveillance task. However grid-based maps that are commonly used map format for mobile robot navigation use a large size of memory for accurate representation of environment. In this reason, grid-based maps are not suitable for path planning of mobile robots using embedded board. In this paper, we present the path planning algorithm that produce a secure path rapidly. The proposed approach utilizes a hybrid map that uses less memory than grid map and has same efficiency of a topological map. Experimental results show that the fast path planning uses only 1.5% of the time that a grid map based path planning requires. And the results show a secure path for mobile robot.

• International Journal of Advanced Culture Technology
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• 제3권1호
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• pp.101-112
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• 2015

Renewable energy resources play an important part in the world's future. Renewable energy sources have the following components: biomass, geothermal, solar thermal, directs solar, wind, tidal and hydropower. Hydropower is still the most efficient way to generate electricity worldwide. Hydropower projects can contribute as a cheap energy source, as well to encourage the development of small industries across a wide range of new technology; furthermore hydropower systems use the energy in flowing and falling water to produce electricity or mechanical energy. Hydropower systems are classified as large, medium, small, mini and micro according to their installed power generation capacity, as do the following components: water turbines, control mechanisms and electrical transmissions. In this article a review of small hydropower systems has been done on the principles surrounding the fundamentals of hydraulic engineering, the fundamentals of hydrology, identification of sites and economic analysis.

• 디지털산업정보학회논문지
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• 제7권4호
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• pp.101-110
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• 2011

The change of digital convergence service environment converted the subject of design into user -centric one instead of developer-centric one, and changed the existing design environment focused on product development into the new user -centric design environment. Accordingly, GUI considering user's usability come to take big shares in those contents being used for multimedia equipments. However, as the present educational contents provide complex interface due to its design produced based on the existing e-learning system, they have many problems in that they don't consider user's usability. Thus this paper aimed to analyze actual examples through GUI elements of educational contents being used for multimedia equipments and attempt to suggest some directions for development. additionally, consider the various characteristics of digital convergence environment and educational contents, and try to set up strategic directions possible to utilize user'-centric interface elements more usefully and properly at the time to produce designs.

• 한국미생물·생명공학회지
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• 제23권5호
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• pp.550-555
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• 1995

A strain of Pseudomonas sp. isolated from soil was shown to produce a high level of extracellular endo-inulinase. In this work, the endo-inulinase gene (inu1) of the bacterial strain was cloned into the plasmid pBR322 by using EcoRI restriction endonuclease and E. coli HB101 as a host strain. One out of 7, 000 transformants obtained from the above cloning experiment formed a clear zone around its colony on the selective medium supplemented with 2.0% inulin after a prolonged incubation at 37$\circ$C and subsequent cold shock treatment. The functional clone was found to carry a recombinant plasmid (pKMG50) with a 3.7 kb genomic insert containing the genetic information for the inulinase activity. The inulinase from E. coli HB101/pKMG50 was proved to be an endo-acting enzyme and produced constitutively in the recombinant E. coli cells. Zymogram of the enzyme from the recombinant cells with inulin substrate indicated that the molecular mass of the active protein was 190 Kd, while that of the endo-inulinase from the Pseudomonas strain was 170 Kd. This size discrepancy suggested that the inulinase from the recombinant E. coli HB101 cells might be the initial product of translation, not the mature form produced in the strain of Pseudomonas sp..