• Applied Biological Chemistry
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• v.37 no.2
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• pp.105-109
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• 1994

Five cytotoxic and antioxidative flavonoids were isolated from the stem bark of Cudrania tricuspidata by consecutive purification using HP-20, silicagel and prep-HPLC. They were identified as taxifolin, orobol, eriodictyol, dihydrokaempferol and steppogenin by means of spectral studies. The antioxidative activities $(IC_{50})$ assayed by TBA method of these compound $1{\sim}5$ to were 6, 3, 3, >50, and $10\;{\mu}g/ml$, respectively. The effect on the growth inhibition $(IC_{50})$ of these compounds against P388 cell line were found to be 0.18, 3.3, 15 and $6.2\;{\mu}g/ml$, respectively in the order of compound 2 to 5.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.395-402
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• 2005

Biologically active recombinant human follicle stimulating hormone (rhFSH) was produced in Chinese hamster ovary cells and purified by a series of chromatographic steps. The chromatographic steps included anion-exchange chromatography (DEAE Sepharose F/F, Q Sepharose F/F), hydrophobic interaction chromatography (Source 15 PHE), and hydroxyapatite chromatography (Macro-Prep ceramic hydroxyapatite type I). A distinctive step of the purification process developed was the use of ZnCl$_2$ for the removal of non-glycosylated or lowly-glycosylated FSH and impurities through co-precipitation with Zn$^{2+}$. Purified rhFSH was identified and characterized by several physicochemical and biological methods such as gel electrophoresis, high-performance liquid chromatography, amino acid analysis, carbohydrate analysis, and biological activity. The overall yield of the purification was ~$30\%$. The rhFSH preparation obtained showed high purity (>$99\%$) and high in vivo potency (>16,000 IU/mg). Carbohydrate analysis suggested that the purified rhFSH contained approximately $40\%$ (w/w) carbohydrate with di­or tri-antennary structure on average, which is somewhat more heavily sialylated than commercially available rhFSH. In conclusion, the results of these analyses established an identity of the purified rhFSH with natural FSH from human pituitary glands, and furthermore, the purified rhFSH preparation showed higher in vivo potency and was slightly more heavily sialylated than commercially available rhFSH.

• Journal of Life Science
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• v.16 no.6
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• pp.989-993
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• 2006

The antimicrobial substance from Pyrola japonica were extracted and isolated. Eighty percent ethanol extract of dried Pyrola japonica was fractionated to hexane, diethyl ether, ethyl acetate and aqueous layer. The hexane-soluble fraction showed the highest inhibitory activity against Bacillus subtilis. Moreover the hexane layer was fractionated into 5 groups by silica gel column chromatography. From the results, group No. 2 ($18{\sim}40$ fractions) showed the highest antimicrobial activity. The group was re-separated to 10 fractions by preparative thin layer chromatography and the peak I as active fraction was isolated by HPLC.

• Korean Journal of Food Science and Technology
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• v.33 no.3
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• pp.389-394
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• 2001

In order to isolate antimicrobial substances from Schizandra chinensis, the dried fruits were extracted with the methanol and the extract showed a strong antimicrobial activity. Also, the methanol exract was further fractionated with hexane, dichloromethane, ethylacetate and buthanol. The ethyl acetate-soluble fraction showed the strongest antimicrobial activity. These fraction were further separated by using various chromatographic methods including thin layer chromatography, silicagel open column chromatography and prep. HPLC. A major component S-EA-5-T1 and S-EA-5-T3 from the ethyl acetate fraction, which showed a strong antimicrobial activity was identified by Mass and NMR spectrometry. Two compounds were isolated and identified as trimethylcitrate and the essential oil of Schizandra chinensis and was estimated as gomisin C, respectively. The growth of S. typhimurium was also inhibited about 1.65 to 2.86 log cycle in minced pork by the addition 1% of Schizandra chinensis extract for 12 days at $4^{\circ}C$. These results suggested that these compounds have a strong potential as a natual food preservatives.

• Journal of Dental Rehabilitation and Applied Science
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• v.22 no.3
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• pp.231-242
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• 2006

Statement of problem: The sealing of the opened dentinal tubules that follows the tooth preparation for the prosthodontic restoration is considered as clinical process to reduce postoperative sensitivity. Purpose: This study investigated the effect of desensitization treatment on shear bond strength of luting cements. Materials and Method: Total 80 dentin specimens were divided into two groups according to the kinds of luting cements. Each groups was further divided into 4 subgroups with AQ $bond^{(R)}$, $Saforide^{(R)}$, Diode laser $MDL-10^{(R)}$ application and without desensitization treatment. After desensitization treatment application, Ni-Cr specimens were luted to dentin surface with Fuji $CEM^{(R)}$ and $Panavia-F^{(R)}$. Specimens were placed in distilled water at $37^{\circ}C$ for 24 hours and shear bond strength between metal and dentin was measured by a universal testing machine. Results: 1. In Fuji $CEM^{(R)}$ cemented groups, the combination of AQ $bond^{(R)}$ showed the greatest strength, followed by diode laser, no desensitizer treatment, and $Saforide^{(R)}$. Both AQ $bond^{(R)}$ and Diode laser groups had a significant difference than no desensitization treatment group and $Saforide^{(R)}$ group(p<0.05). 2. In $Panavia-F^{(R)}$ cemented groups, the combination of Diode laser showed the greatest strength, followed by AQ $bond^{(R)}$, $Saforide^{(R)}$, and No desensitization treatment. All desensitization treatment groups had a significant difference than no desensitization treatment group(p<0.05). 3. All $Panavia-F^{(R)}$ groups showed a significant higher shear bond strength than all Fuji $CEM^{(R)}$ groups(p<0.05). Conclusion: The results of this study showed possibility of bond strength increase after desensitization treatment. The application of desensitization treatments like AQ $bond^{(R)}$, $Saforide^{(R)}$, and Diode laser $MDL-10^{(R)}$ have advantages in exposed dentin surface after tooth prep.

• Journal of Life Science
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• v.17 no.1 s.81
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• pp.39-44
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• 2007

The Rqh1 gene is essential for vegetative growth in fission Yeast. The rqh1 mutant showed that sensitivity of DNA damaging agent, a wild range of phenotype including abnormal gene expression and cell elongation. This result showed that the rqhl-overexpression cell was sensitivity to DNA damaging agent like rqhl mutant. When Rqh1 have an over-expression by $nmt1^+$ promoter of pREP vector, rqh1 mutant DNA damaging agent sensitivity could be compensated. We isolated two strong mutant containing complementation gene, rqh156 and rqh172, respectively. This result observed that the DNA damaging agent sensitivity of rqhl mutant was complemented by the expression of rqh156 and rqh172. They induced mRNA expression in a dose-dependent manner HU, MMS and UV. The HU sensitivity of the rqhl was complemented by the expression of rqh156 and rqh172. The mRNA expression of rqh156 decreased on HU dose dependent but the mRNA expression of rqh172 did not decrease on HU dose dependent. The MMS and W sensitivity of the rqhl was complemented by the expression of rqh156 and rqh172. These results indicate that the isolated rqhl gene may play an important role in DNA metabolism.

• The Journal of the Convergence on Culture Technology
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• v.6 no.1
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• pp.413-418
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• 2020

The travel pattern of tourists for the development of the tourist course is designed to collect and analyze tourist information based on the big data of the carrier to improve the quality of the tourist course. In particular, the analyzed data is used to derive empirical data that can estimate the effect of tourists' inflow into tourism, and to utilize the information as basic data for the development of the tourist course. In addition, the travel pattern of tourists for the development of regional tourism courses is to collect and analyze information on the route and duration of tourists' travel based on big data collected by telecom operators, credit card companies and other data to improve the quality of tourist course development, and to derive empirical data to estimate the effect of tourist inflow through the analyzed data, based on the characteristics of the tourism course and the data needed for the development of new tourist courses in the future.

• Convergence Security Journal
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• v.19 no.3
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• pp.109-115
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• 2019

The North's short-range projectiles and missiles are the Iskander-class missiles of the S-300 series, with a range of 270-420 kilometers and an altitude of 40-50 kilometers, making it very difficult to respond with South Korea's detection radar or striking weapons. The North's handling of the Seoul sea of fire also makes it very urgent for the South to deploy the weapons to power or introduce them as soon as possible, as it can identify its intention to strike the Seoul metropolitan area by equipping such short-range rockets and missiles with nuclear or chemical weapons. We will be prepared to prep are for reckless provocations by securing our own technology by continuously developing the Korean missile defense system and striking system, Kill Chain, which is designed to defend short-range missiles in the long-term, and securing our own technology.

• Genomics & Informatics
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• v.2 no.1
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• pp.30-35
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• 2004

To investigate the XIST gene expression and its effect in a Klinefelter's patient, we used Klinefelter's syndrome (XXY) patient with azoospermia and also used a normal male (XY) and a normal female (XX) as the control, We were performed cytogenetic analysis, Y chromosomal microdeletion assay (Yq), semi-quantitative RT-PCR, and the Northern blot for Klinefelter's syndrome (KS) patient, a female and a male control, We extracted total RNA from the KS patient, and from the normal cells of the female and male control subjects using the RNA prep kit (Qiagen), cDNA microarray contained 218 human X chromosome-specific genes was fabricated. Each total RNA was reverse transcribed to the first strand cDNA and was labeled with Cy-3 and Cy-5 fluorescein, The microarray was scanned by ScanArray 4000XL system. XIST transcripts were detected from the Klinefelters patient and the female by RT-PCR and Northern blot analysis, but not from the normal male, In the cDNA microarray experiment, we found 24 genes and 14 genes are highly expressed in KS more than the normal male and females, respectively. We concluded that highly expressed genes in KS may be a resulted of the abnormal X inactivation mechanism.

• Analytical Science and Technology
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• v.7 no.4
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• pp.547-554
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• 1994

Ceramide components were detected from a native mushroom, "Phellinus ribis" growing in Korea. Cerebroside fractions were isolated by silica column chromatography. The ceramide fraction were purified by preparative TLC and their constituents were analyzed by using GC-MS and FAB-MS. The fatty acid components consisted of mainly hydroxy fatty acids($C_{22:0}$, $C_{25:0}$, $C_{24:0}$, $C_{23:0}$) : The major components of long-chain base were trihydroxy-bases, principally 4-hydroxy sphinganin analogues.