• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1061-1065
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• 2001

The antibacterial effects of water extracts of Scutellariate Radix (a dried root of Scutellaria baicalensis GEORGI) and its major flavonoid components, Baicalin and Baicalein, on Salmonella typhimurium, a representative enteric pathogen, were studied. Through a Kriby-Bauer disc analysis, the growth-inhibition activity of Scutellariae Radix against. S. typhimurium was found to be compatible with commercial antibiotics, such as ampicillin, chloramphenicol, and streptomycin. In contrast, the growth of a nonpathogenic E. coli strain was unaffercted by Scutellariae Radix. To examine the effect of polyphosphate kinase (ppk), a putative virulence factor, on the antibacterial activity of Scutellariae Radix, the growth profile of a ppk mutant of S. typhimurium was investigated in a tryptic soy broth containing different concentrations of water extracts of Scutellariae Radix. The ppk mutant was able to grow in 6 mg/ml of water extracts of Scutellariae Radix, whereas in 6 mg/ml of water extracts of Scutellariae Radix, whereas the wild-type could not, implying that the inactivation of ppk made S. typhimurium more resistant to the antibacterial activity of Scutellariae Radix. No enhanced resistance was observed in a ppk mutant of S. typhimurium complemented with a ppk expression vector. The attenuation of the virulence by ppk inactivation was also observed in a virulence assay using BLAB/c mice. Neither Baicalin nor Baicalein exhibited any growth-inhibition activity against S. typhimurium. The water extracts of Scutellariae Radix stimulated the transcription of ppk, especially in the early growth-stage of S. typhimurium.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.7 no.4
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• pp.355-362
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• 2017

Recently, UAV(Unmanned Aerial Vehicle) have been utilized in various fields, including surveys, mapping, and spatial analysis, depending on the increase in demand for spatial information and UAV is receiving a lot of attention due to rapid data acquisition and economic viability. In this study, the applicability of UAV image images was analyzed for urban disaster prevention. UAV images were acquired for the study area and digital surface model and ortho image were generated through data processing. Also, the process using PPK(Post Processed Kinematic) GNSS method is compared with existing method. Through the research, it was able to effectively deploy urban disaster prevention information about the target area, and displayed the effectiveness of the methods for efficient comparison with existing unmanned aerial photogrammetry. If the PPK technique is applied to thethe disaster prevention field, it is expected that the work flow in the field of rapid data acquisition and disaster prevention data construction can be greatly improved.

• KAPE Magazine
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• s.172
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• pp.34-37
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• 2010

• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.139-145
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• 2003

The gene encoding Thermus caldophilus GK24 polyphosphate kinase (Tca PPK) was cloned and sequenced. The gene contains an open reading frame encoding 608 amino acids with a calculated molecular mass of 69,850 Da. The deduced amino acid sequence of Tca PPK showed a 40% homology to Escherichia coli PPK, and $39\%$ to Klebsiella aerogenes PPK. The Tca ppk gene was expressed under the control of the T7lac promoter on pET-22b(+) in E. coli and its enzyme was purified about 70-fold with $36\%$ yield, following heating and HiTrap chelating HP column chromatography. The native enzyme was found to have an approximate molecular mass of 580,000 Da and consisted of eight subunits. The optimum pH and temperature of the enzyme were 5.5 and $70^{\circ}C$, respectively. A divalent cation was required for the enzyme activity, with $Mg^2+$ being the most effective.

• Journal of The Geomorphological Association of Korea
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• v.28 no.4
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• pp.41-52
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• 2021

With the popularization of drones and the ease of use of the Global Navigation Satellite System (GNSS), drone photogrammetry for terrain information has been widely used. Drone photogrammetry enables the realization of high-accuracy three-dimensional topography for the entire area with less effort and time compared to the past direct survey using GNSS or total station. From 3-D topographic data, various topographical analysis is possible. To improve the accuracy of drone photogrammetry, direct GCP surveying in the field is essential, and the numbers and reasonable positioning of GCPs are very important. In the case of beaches or tidal flats on the west coast of Korea, the numbers and location of GCPs are important factors in efficient drone photogrammetry because of the size of the area, difficulties of movement, and the risk from tides. If the RTK (Real-time kinematic) or PPK (Post-processed kinematic) method is used, the increased accuracy of the drone's location enables high-accuracy photogrammetry with a small number of GCPs. This study presents an efficient drone photogrammetry method in terms of time and economy by comparing and analyzing the results of drone photogrammetry using Non-PPK with low-cost PPK-Kit, based on the tests of various numbers and locations of GCPs in the university field including various slopes and structures like coastal terrain.

• Journal of Life Science
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• v.10 no.4
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• pp.397-402
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• 2000

Polyphosphate kinase catalyzes the formation of polyphosphate from ATP. To understand the mechanism of phosphate accumulation, the Serratia marcescens gene encoding ppk was cloned from the genomic library by the method of Southern hybridization. The hybridization positive DNA fragment region from pDH3 was subcloned into the expression vector. The ppk gene product, a polypeptide of 75 kDa, was confirmed by SDS-PAGE. Expression of the Serratia marcescens ppk is regulated by the catabolite repression system. The enzyme activity polyphosphate kinase was increased in the E. coli strain harboring plasmid pMH4 with ppk gene.

• Molecules and Cells
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• v.39 no.7
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• pp.573-579
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• 2016

During copulation, male Drosophila transfers Sex Peptide (SP) to females where it acts on internal sensory neurons expressing pickpocket (ppk). These neurons induce a post-mating response (PMR) that includes elevated egg-laying and refractoriness to re-mating. Exactly how ppk neurons regulate the different aspects of the PMR, however, remains unclear. Here, we identify a small subset of the ppk neurons which requires expression of a pre-mRNA splicing factor CG3542 for egg-laying, but not refractoriness to mating. We identify two CG3542-ppk expressing neurons that innervate the upper oviduct and appear to be responsible for normal egg-laying. Our results suggest specific subsets of the ppk neurons are responsible for each PMR component.

• The Journal of Asian Finance, Economics and Business
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• v.7 no.10
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• pp.491-499
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• 2020

The implementation of PPK-BLU in Indonesia was not followed by a comprehensive change in aspects of organization, human resources, and finance. Based on this background, this study aims to provide a comprehensive evaluation of the BTIP after the implementation of the PPK-BLU policy in the implementation of telecommunications services and information to the public in Indonesia. This research used integration of the McKinsey 7S method, Analytical Hierarchy Process, and Likert scale. The integration of these methods can provide a detailed quantitative analysis. Based on the overall evaluation results of carrying out the PPK BLU, BTIP has a performance value of 81.195%, which puts it within the Good category. The McKinsey 7S elements used as a whole have the following values: one aspect is categorized as Very Good, namely, the strategy aspect with a value of 91.025%; two aspects are categorized as Good (structural aspects with a value of 86.857%, and skill aspects with a value of 81.432%); three aspects are categorized as Adequate (style aspect with a value of 76.441%, staff aspect with a value of 78.605%, and share value aspect with a value of 76.331%); one aspect is categorized as Bad, that is the system aspect with a value of 67.503%.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1527-1535
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• 2009

Polyphosphate (polyP) plays diverse physiological functions in prokaryotes and eukaryotes, but most of their detailed mechanisms are still obscure. Here, we show that deletion of polyphosphate kinase (PPK), the principal enzyme responsible for synthesis of polyP, resulted in augmented expression of cAMP receptor protein (CRP) and rpoS and lowered $H_2O_2$ sensitivity in Salmonella Typhimurium ATCC14028. The binding of cAMP-CRP complex to rpoS promoter and further stimulation of its transcription were proved through electrophoretic mobility shift assay, lacZ fusion, and exogenous cAMP addition, respectively. The rpoS expression increased in cpdA (cAMP phosphodiesterase coding gene) mutant, further suggesting that cAMP-CRP upregulated rpoS expression. These results demonstrate that PPK affects oxidative stress response by modulating crp and rpoS expression in S. Typhimurium.

• 프린팅코리아
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• v.10 no.5
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• pp.84-85
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• 2011