• Journal of the Korean institute of surface engineering
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• v.26 no.6
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• pp.327-333
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• 1993

The effect of heat treatment on the adhesion between Cu-18wt% Cr film and polyimide has been studied by using T-peel test, AES, and XRD. Cu-18wt% Cr alloy and pure Cu films were sputter deposited onto pol-yimide. Cu was electroplated before and after heat treatment at $400^{\circ}C$ for 0.5 hr and 2 hrs respectively. The adhesion of metal film onto polyimide was considerably good before heat treatment, but heat treatment re-duced the peel adhesion strength in all specimens. The reduction in adhesion in adhesion strength values in the specimens which were plated after heat treatment was mainly due to Cr-O rich pahse formed in the metal/polyimide in-terface. In the specimens which were heat treated after plating, the enhanced ductility in the metal films con-tributes the peel adhesion strength by increasing the amount of deformation in metal strips.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.28-28
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• 2015

본 연구는 KICT X밴드 이중편파 레이더 관측자료를 이용하여, 우리나라 기상조건에서 X밴드 이중편파 레이더 $K_{DP}$ 추정 기법에 따른 강우강도 추정의 차이를 비교 검토하였다. 이중편파 레이더로 $K_{DP}$ 기반의 정확도 높은 강우강도 분포 추정에 있어, $K_{DP}$ 추정의 방법에 따라서 추정된 $K_{DP}$의 공간 분포는 상당한 차이를 보이게 된다. 추정 기법만을 볼 때, 이는 주로 ${\Phi}_{DP}$로부터 기울기를 산정하는 방법과 기울기를 산정하는 윈도우의 크기에서 차이가 발생한다. 윈도우의 크기는 일반적으로 호우의 강도에 따라 달리 적용하는 것이 $R-K_{DP}$ 관계에서 보다 정확도가 높은 것으로 알려져 있다. $K_{DP}$ 산정 기법에 따른 차이 검토를 위해 기울기 산정 기법, 필터(FIR) 상수 및 ${\Phi}_{DP}$ 기울기 산정을 위한 윈도우 크기에 따른 강수 추정의 정확도를 검토하였다.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.55.3-55.3
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• 2019

우리나라 최초의 우주탐사 프로그램인 Korea Pathfinder Lunar Orbiter (KPLO)는 1년의 임무기간동안 달과 달 주변의 우주환경에 대한 과학탐사 임무를 수행할 예정이다. 이를 위해서 1개에 기술 검증장비와 고해상도 카메라를 포함한 5개의 과학장비를 탑재할 예정이다. 이 중 고해상도 카메라인 LUTI(LUnar Terrain Imager)와 국내에서 개발한 3개의 과학탑재체(KGRS;감마선분광기, KMAG;자기장측정기, PolCam;광시야 편광카메라)가 획득한 과학자료는 일정기간(통상 1년)동안 비공개로 검보정이 이루어진 후 일반에게 공개(Public release)할 예정이다. 이러한 과학자료의 공개와 관리를 위해서 한국항공우주연구원은 KPLO 심우주 지상시스템 내에 과학자료의 공개 및 관리를 위한 KARI Planetary Data System(KPDS)을 개발하고 있다. KPDS는 미국 NASA의 PDS에서 개발하여 유럽, 일본 등에서 이미 행성탐사 과학자료의 표준으로 활용하고 있는 PDS4 표준을 준수하는 과학자료를 제공할 것이다. 본 발표를 통해서 KPDS의 운영개념과 과학자료 관리계획, 그리고 KPDS의 개발현황을 천문학계와 공유하여 KPLO에 의해서 획득된 과학자료가 많은 과학자들이 활용하여 높은 과학적 성과를 낼 수 있기를 기대한다.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.41.2-41.2
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• 2019

The B-fields In STar-forming Region Observations (BISTRO 1 and 2) is a large program of the James Clerk Maxwell Telescope (JCMT) using SCUBA-2 and POL-2, starting in 2016. We aim to study the roles of magnetic fields in star formation by observing 32 fields of nearby low-mass and high-mass star forming regions. The angular resolution and the wavelength provided by JCMT (14 arcsecond at 850 micrometer) are ideal to investigate the intermediate scales of magnetic fields (1000-20000 au) associated in cold dense cores and filaments. We report the current status of this project and discuss the magnetic fields of the Serpens Main molecular cloud in which several filaments with various physical properties have been identified.

• The Korean Journal of Pesticide Science
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• v.2 no.3
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• pp.64-69
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• 1998

New thirty derivatives of 5,6-dihydro-2-trifluoromethyl-1,4-oxathiin carboxanilide as substrate(S) were synthesized and their fungicidal activities in vivo against rice sheath blight(Rhizoctonia solani) and wheat leaf rust(Puccinia recondita) were examined. The structure activity relationships(SAR) between the activities($pI_{50}$) and a physicochemical parameters of substituents(X) at the phenylcarbamoyl group were analyzed using the adaptive regression analysis method. The 3-methoxy, 11, 3-isopropyloxy, 13 and 3-isopropyl substituent, 25 as X on the phenylcarbamoyl group exhibited the most highest fungicidal activity against the two fungi. The fungicidal potency of the (S) against Puccinia recondita was higher than Rhizoctonia solani. In case of Rhizoctonia solani, the molecular hydrophobicity(${\pi}>0$) and resonance effect(R<0) by meta-alkyl substitutents with electron donating were important factors in determining fungicidal activity. And the HOMO energy(HOMO>0), ABSQ, sum of absolute values of the atomic charges on each atom and specific polarizability(Sp.Pol<0) of (S) were significantly influential towards fungicidal activity against Puccinia recondita.. The interaction between (S) and receptor agonist from the based on SAR studies proceeds through charge-control reaction, and conditions to show higher activity has been also discussed.

• Microbiology and Biotechnology Letters
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• v.37 no.1
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• pp.17-23
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• 2009

The gene encoding Thermus thermophilus HJ6 DNA polymerase (Tod) was cloned and sequenced. The open reading frame (ORF) of the Tod gene was composed of 2,505 nucleotides and encoded a protein (843 amino acids) with a predicted molecular weight of 93,795 Da. The deduced amino acid sequence of Tod showed 98% and 86% identities to the Thermus thermophilus HB8 DNA pol and Thermus aquaticus DNA pol, respectively, The Tod gene was expressed under the control of the bacteriophage $\lambda$ promoters PR and PL on the expression vector pJLA503 in Escherichia coli strain BL21 (DE3) codon plus. The expressed enzyme was purified by heat treatment, $HiTrap^{TM}$ Q column, and $HiPrep^{TM}$ Sephacryl S-200 HR 26/60 column chromatographies. The optimal temperature and pH for DNA polymerase activity were found to be $75{\sim}80^{\circ}C$ and 9.0, respectively. The optimal concentrations of $Mg^{2+}$ and $Mn^{2+}$ were 2.5 mM and 1 mM, respectively. The enzyme activity was activated by divalent cations, and was inhibited by monovalent cations. The result of the PCR experiment with Tod DNA polymerase indicates that this enzyme might be useful in DNA amplification and PCR-based applications.

• Reproductive and Developmental Biology
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• v.30 no.3
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• pp.181-187
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• 2006

In recent years the number of patients waiting for organ transplantation has greatly outpaced the supply of human organs available, which leads to a renewed interest in pig-to-human xenotransplantation as an alternative. However, one of the biggest barriers in the xenotransplantation is presence of porcine endogenous retroviruses(PERV) that can infect human cells. In this study, to present a possible solution for this problem we tried to inhibit expression of PERVs using shRNAs(short hairpin RNA) at the level of RNA synthesis and virus release. The shRNA targeting the sequence of PERV A, B type was cloned into pSIREN-RetroQ vector under the control of polymerase-III U6-RNA gene promoter. Quantitative real-time PCR was performed to detect my alterations in mRNA production of PERV A, B targeted by the shRNA in each done. Depending on the target sequence of the shRNA, the transcription of PERV was decreased to as much as 4% and the number of progeny viruses was reduced to less than 1/200,000. Transgenic pigs producing such shRNAs may result in a highly reduced PERV expression in cells and organs, which is a prerequisite for safe xenotransplantations.

• Molecules and Cells
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• v.38 no.1
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• pp.1-13
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• 2015

Recent technical developments have transformed how neuroscientists can probe brain function. What was once thought to be difficult and perhaps impossible, stimulating a single set of long range inputs among many, is now relatively straight-forward using optogenetic approaches. This has provided an avalanche of data demonstrating causal roles for circuits in a variety of behaviors. However, despite the critical role that neuropeptide signaling plays in the regulation of behavior and physiology of the brain, there have been remarkably few studies demonstrating how peptide release is causally linked to behaviors. This is likely due to both the different time scale by which peptides act on and the modulatory nature of their actions. For example, while glutamate release can effectively transmit information between synapses in milliseconds, peptide release is potentially slower [See the excellent review by Van Den Pol on the time scales and mechanisms of release (van den Pol, 2012)] and it can only tune the existing signals via modulation. And while there have been some studies exploring mechanisms of release, it is still not as clearly known what is required for efficient peptide release. Furthermore, this analysis could be complicated by the fact that there are multiple peptides released, some of which may act in contrast. Despite these limitations, there are a number of groups making progress in this area. The goal of this review is to explore the role of peptide signaling in one specific structure, the bed nucleus of the stria terminalis, that has proven to be a fertile ground for peptide action.

• Proceedings of the KSRS Conference
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• 1999.11a
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• pp.456-461
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• 1999

It is very important for monitoring the interannual variability of sea ice extents in the Okhotsk Sea because the global warming has firstly appeared around the Okhotsk Sea, locating around the southernmost region of sea ice cover in the Northern Hemisphere. In order to develop the sea ice concentration algorithm by microwave sensors onboard satellite, electromagnetic properties of sea ice in the Okhotsk Sea, therefore, were observed by airborne microwave radiometer (AMR), which has the same frequencies as AMSR (Advanced Microwave Scanning Radiometer), ADEOS-II, launching on November, 2000. On this study, it is discussed how to make the image of AMR-EFOV and the video image with nadir angle under flight at the same time, and superimpose the brightness temperature data by AMR-EFOV on the video mosaiced images. For comparing SPOT image, it is clearly that the variation of brightness temperature is small in 89GHz V-pol without the sea ice types and increase at the lower frequency-band.

• Korean Journal of Microbiology
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• v.29 no.2
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• pp.75-79
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• 1991

The yeast L-A virus (4.6 kb dsRNA genome) encodes the major coat protein and a "gag-pol" fusion minor coat protein that separately encapsidate itself and $M_{1}$, a 1.8 kb dsRNA satellite virus encoding a secreted protein toxin (the killer toxin). The teast chromosomal SKI genes prevent viral cytopathology by lowering the virus copy number. Thus, $ski^{-}$ mutants are ts and cs for growth. We transformed a ski2-2 virus-infested mutant with a yeast bank in a high copy cloning vector and selected the rare healthy transformants for analysis. One type of transformant segregated M-O L-A-O cells with high frequency. Elimination of the DNA clone from the ski2-2 strain eliminated this phinotype and introduction of the DNA clone recovered from such transformants into the parent ski2-2 strain, or into ski3 or ski6 mutants gave the same phenotype. This killer-curing phenotype was due to the curing of the helper L-A dsRNA virus. The 6.5 kb insert only had this activity when carried on a high copy vector and in $ski^{-}$ cells (not in $SKI^{+}$ cells). This 6.5 kb insert acts as a mutagen on L-A dsRNA producing a high rate of deletion mutations.mutations.