• 대한본초학회지
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• 제38권4호
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• pp.45-52
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• 2023

Objectives : Fine dust caused by environmental pollution cause oxidative damage and skin aging. In this study, The possibility of using the Codium fragile extract (CFE) as an anti-aging product for skin improvement was evaluated by confirming the protective effect of skin cells from PM10 (particulate matter 10) through inhibition of ROS and MMPs. Methods : In this study, elastase and collagenase inhibitory activities were evaluated. Cell viability was evaluated by treating keratinocytes (HaCaT cell line) with CFE at various concentrations. The cytoprotective effect from PM10 in keratinocyteswas evaluated using the 3-[4,5-dimethylthiazol]-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay. ROS (reactive oxygen species) was measured in keratinocytes damaged by PM10 using DCF-DA (2′,7′-dichlorofluorescin diacetate) fluorescence staining. As an anti-aging effect of CFE, MMP-1 (matrix metalloproteinase-1) and MMP-1 (matrix metalloproteinase-9) inhibitory activities were evaluated. Results : As a result, CFE decreased the activity of elastase and collagenase. As a result of evaluating the toxicity of CFE, it is non-toxic at a concentration of 10 to 80 ㎍/㎖. Although cell viability of HaCaT cells treated with PM10 decreased, cell viability increased by 38% when treated with CFE 80 ㎍/㎖. Also, ROS decreased by 8.4%, and MMP-1 and MMP-9 decreased at CFE 80 ㎍/㎖. Conclusions : CFE showed excellent cell protection effect, and it is considered that it can be used in anti-aging products for skin improvement by effectively inhibiting ROS and MMPs from keratinocyte damage caused by fine dust.

• Journal of Preventive Medicine and Public Health
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• 제57권2호
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• pp.108-119
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• 2024

Objectives: Abrupt changes in air pollution levels associated with the coronavirus disease 2019 (COVID-19) outbreak present a unique opportunity to evaluate the effects of air pollution on influenza risk, at a time when emission sources were less active and personal hygiene practices were more rigorous. Methods: This time-series study examined the relationship between influenza cases (n=22 874) and air pollutant concentrations from 2018 to 2021, comparing the timeframes before and during the COVID-19 pandemic in and around Thailand's Khon Kaen province. Poisson generalized additive modeling was employed to estimate the relative risk of hospitalization for influenza associated with air pollutant levels. Results: Before the COVID-19 outbreak, both the average daily number of influenza hospitalizations and particulate matter with an aerodynamic diameter of 2.5 ㎛ or less (PM_2.5) concentration exceeded those later observed during the pandemic (p<0.001). In single-pollutant models, a 10 ㎍/m³ increase in PM_2.5 before COVID-19 was significantly associated with increased influenza risk upon exposure to cumulative-day lags, specifically lags 0-5 and 0-6 (p<0.01). After adjustment for co-pollutants, PM_2.5 demonstrated the strongest effects at lags 0 and 4, with elevated risk found across all cumulative-day lags (0-1, 0-2, 0-3, 0-4, 0-5, and 0-6) and significantly greater risk in the winter and summer at lag 0-5 (p<0.01). However, the PM_2.5 level was not significantly associated with influenza risk during the COVID-19 outbreak. Conclusions: Lockdown measures implemented during the COVID-19 pandemic could mitigate the risk of PM_2.5-induced influenza. Effective regulatory actions in the context of COVID-19 may decrease PM_2.5 emissions and improve hygiene practices, thereby reducing influenza hospitalizations.

• Toxicological Research
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• 제27권3호
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• pp.161-166
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• 2011

Carbon black, a particulate form of pure elemental carbon, is an industrial chemical with the high potential of occupational exposure. Although the relationship between exposure to particulate matters (PM) and cardiovascular diseases is well established, the cardiovascular risk of carbon black has not been characterized clearly. In this study, the cytotoxicity of carbon black to vascular smooth muscle and endothelial cells were examined to investigate the potential vascular toxicity of carbon black. Carbon black with distinct particle size, N330 (primary size, 28~36 nm) and N990 (250~350 nm) were treated to A-10, rat aortic smooth muscle cells and human umbilical vein endothelial cell line, ECV304, and cell viability was assessed by lactate dehydrogenase (LDH) leakage assay. Treatment of carbon black N990 resulted in the significant reduction of viability in A-10 cells at 100 ${\mu}g$/ml, the highest concentration tested, while N330 failed to cause cell death. Cytotoxicity to ECV304 cells was induced only by N330 at higher concentration, 200 ${\mu}g$/ml, suggesting that ECV304 cells were relatively resistant to carbon black. Treatment of 100 ${\mu}g$/ml N990 led to the elevation of reactive oxygen species (ROS) detected by dichlorodihydrofluorescein (DCF) in A-10 cells. Pretreatment of antioxidants, N-acetylcysteine (NAC) and sulforaphane restored decreased viability of N990-treated A-10 cells, and N-acetylcysteine, but not sulforaphane, attenuated N990-induced ROS generation in A-10 cells. Taken together, present study shows that carbon black is cytotoxic to vascular cells, and the generation of reactive oxygen contributes to the development of cytotoxicity. ROS scavenging antioxidant could be a potential strategy to attenuate the toxicity induced by carbon black exposure.

• 한국해양환경ㆍ에너지학회지
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• 제9권4호
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• pp.203-215
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• 2006

'한국 남해 중앙부 해역의 표층퇴적물내 유기물의 시 공간적 분포특성을 파악하고자 2002년 4월부터 2003년 1월까지 15개 정점을 대상으로 격월간격으로 현장조사를 실시하였다. 저층해수(B-1m) 수온과 염분 그리고 표층퇴적물의 니질 함량과 함수율은 각각 $8.1{\sim}23.4^{\circ}C,\;29.2{\sim}34.5\;psu,\;71.2{\sim}99.9%$ 및 $38.7{\sim}68.9%$의 범위를 보였다. 강열감량(IL), 식물색소량(phaeopigment), 입자성 유기탄소(POC), 입자성 유기질소(PON) 및 화학적산소요구량(CODs)은 각각 $3.9{\sim}12.5%,\;1.58{\sim}29.51\;{\mu}g/g-dry,\;3.12{\sim}13.01\;mgC/g-dry,\;0.49{\sim}2.0\;mgN/g-dry$ 그리고 $9.6{\sim}44.05\;mgO_2/g-dry$의 범위를 보였다. 유기물의 공간적인 분포는 육지와 인접한 연안역보다 수심이 깊은 외양역에서 높은 유기물량을 나타내었다. 시간적으로는 저수온기보다 고수온기에 유기물량이 증가하였다. 유기물 기원은 C/N ratio가 평균 6.44(${\pm}0.51$)로 나타나 해양자체 생산에 의한 생물기원 유기물에 게 의존하고 있으나, 비교적 높은 POC/phaeopigment ratio로 부터 전체적으로 식물플랑크톤보다 쇄설성 유기물질에 의한 조성비가 높음을 알 수 있었다. 주성분분석 결과 누적 기여율 73.2%의 제 2주성분까지 도출되었다. 얻어진 인자부하량으로부터 제 1주성분은 '환경요인에 따른 표층퇴적물의 유기물 집적정도(57.3%)'를, 제 2주성분은 '해양의 생유기물에 의한 유기물 척도(15.9%)'로 판단되었다. 정점별 득점 분포로부터 남해 중앙부 해역의 표층퇴적물 환경은 유기물 함량과 퇴적물내 식물플랑크톤에 의한 유기물 조성 비율에 따라 4개의 해역으로 구분되었다.

• Tuberculosis and Respiratory Diseases
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• 제60권4호
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• pp.426-436
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• 2006

연구배경: 본 연구는 교통량이 많은 서울의 한 대로변에서 채취된 복합적인 미세분진이 염증반응이 일어날 때 형성되는 것으로 알려진 NO와 NO를 형성하는 iNOS발현 및 NO에 의하여 생성이 증가되는 nitro-tyrosilated-protein의 발현에 미치는 영향을 알아보고자 SPF 흰쥐와 염증성 흰쥐에서 분리된 폐포대식세포와 그람음성 세균의 세포벽 성분인 LPS에 감작시켜 분석하였다. 방법: SPF 흰쥐와 염증성 흰쥐의 폐포대식세포에서 PM을 농도별로 처리하였을 때와 동일한 농도에서 배양시간을 달리하였을 때 분비되는 NO를 Griess reaction 방법으로 측정하였고, iNOS와 nitrotyrosilated-protein의 발현을 세포면역화학염색법과 western blot 분석법으로 확인하였다. 결과: PM의 단독투여 및 LPS와 PM을 혼합하여 투여하였을 때 NO의 생성 및 iNOS와 nitrotyrosilated-protein의 발현을 확인할 수 있었고, 그 효과는 LPS를 단독투여 하였을 때보다 발현이 증가함을 확인할 수 있었다. 한편, 폐 내 자연적 염증성 증상이 있는 흰쥐에서 분리된 폐 내 대식세포는 PM의 농도 증가에 따라 비례하여 NO의 형성을 증가시켰다. 결론: 본 실험에 사용된 PM은 그 자체만으로도 SPF 흰쥐와 폐렴증상이 있는 흰쥐 BAL cell에서 NO의 생성을 증가시켰고, LPS가 처리된 세포들에 PM을 병용하여 투여하였을 경우에는 NO의 생성과 iNOS와 nitrotyrosilated-protein의 발현이 유의하게 상승되었다.

• 한국생산제조학회지
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• 제8권4호
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• pp.13-18
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• 1999

Recently among after-treatment devices which have high possibility of utility diesel oxidation catalyst(DOC) is concerned over the world. DOC oxidizes pollutants by means of activate-reaction during by-passing in the catalyst in doing so conversion efficiency of PM, CO and HC is high and this device does not have an effect on engine performance because back pressure is not nearly increased, But as a small amount of sulfur content in fuel is oxidized it makes sulfate which is absorbed on the surface of catalyst. So in this study the experiment is carried out by means of using ordinary fuel(0.1wt%) and low sulfur fuel(0.05wt%) with DOC and the emission gas of diesel engine is measured.

• 에너지공학
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• 제14권4호
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• pp.226-231
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• 2005

Essentially combination of spark ignition and compression ignition engines, the HCCI engine exhibits low NOx and Particulate Matter (PM) emissions as well as high efficiency under part load. This paper is concerned with the Homogeneous Charge Compression Ignition (HCCI) engine as a new concept in engines and a power source for future automotive applications. In this research, a 4 cylinder diesel engine was converted into a HCCI engine, and propane was used as the fuel. The purpose of this research is to show the effects of fuel flow rate and the temperature of the intake manifold on the performance and exhaust of an HCCI engine.

• 동력기계공학회지
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• 제15권3호
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• pp.5-11
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• 2011

디젤기관의 경우는 종래부터 직분식이 주류를 이루었고, 근래에는 분사압력의 고압화가 진행중이다. 분사압력의 고압화에 의해 연소효율의 향상 및 배출가스중의 입자상물질(PM:Particulate Matter)의 저감을 유도하고 있으나, 연소가스의 고온화로 인해 질소산화물(NOx:Nitrogen Oxides)은 증가한다. 따라서, 분사기간의 지연(Retard)이나 파일럿분사(Pilot injection)등의 혼합기제어에 의해 질소산화물의 저감을 꾀하고 있다. 이와 같이 디젤기관에 있어서도 혼합기 형성의 최적화에 의한 연소제어를 시도하는 수법이 중시되고 있고, 이를 위해서는 디젤분무 구조에 기초한 혼합기의 형성기구에 대한 규명이 매우 중요하다. 그러므로 본 연구에서는 보다 고도의 혼합기형성 제어를 위한 기초연구로서 고온 고압장에서의 증발디젤자유분무구조를 해석하였으며, 계측영역은 연료와 주위기체와의 혼합이 활발히 진행되는 분무의 하류영역으로 설정하고, 입자화상속도측정법(particle Image Velocimetry:PIV)을 이용한 분무의 유동해석을 기초로 증발 디젤분무의 구조 해석을 행하였다. 실험조건으로서 분사압력을 72MPa, 112MPa로 각각 변화시켰다.

• Journal of Mechanical Science and Technology
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• 제18권11호
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• pp.2049-2057
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• 2004

In this study, the combustion characteristics and durability of a diesel engine using emulsified fuel was investigated. Water was used in oil type emulsified fuel. In order to understand the effect of emulsified fuel in a wide range of engine running conditions, D-13 mode was selected as a test condition, and a durability test was included to understand the long-term effect of water. Combustion pressure in a cylinder, exhaust emissions, specific fuel consumption, sound level and maximum torque were measured. NOx and PM were simultaneously reduced and the specific fuel consumption was increased and decreased at low and high loads, respectively. There was no trouble and any damage on the parts of the cylinder during a 500 hour durability test.

• International Journal of Automotive Technology
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• 제5권3호
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• pp.195-200
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• 2004

This paper is concerned with the Homogeneous Charge Compression Ignition (HCCI) engine as a new concept in engines and a power source for future automotive applications. Essentially a combination of spark ignition and compression ignition engines, the HCCI engine exhibits low NOx and Particulate Matter (PM) emissions as well as high efficiency under part load. The objective of this research is to determine the effects of Exhaust Gas Recirculation (EGR) rate on the combustion processes of HCCI. For this purpose, a 4-cylinder, compression ignition engine was converted into a HCCI engine, and a heating device was installed to raise the temperature of the intake air and also to make it more consistent. In addition, a pressure sensor was inserted into each of the cylinders to investigate the differences in characteristics among the cylinders.