• Proceedings of the Optical Society of Korea Conference
• /
• 2002.07a
• /
• pp.158-159
• /
• 2002

오늘날에도 RSA암호문은 현대 암호에 대부분 적용될 정도로 안전하다고 생각되어왔다. 또한 PGP(Pretty Good Privacy)라는 가장 대중적인 암호화 소프트웨어도 RSA 원리를 기반으로 하고 있다. 그러나 RSA암호화체계는 주요한 결점이 있다. 인수분해하는 것이 ＂어렵다＂라고 하지만, 결코 증명되지 않았고, 또한 인수분해를 수행하는 아주 빠른 알고리즘의 존재가 전혀 불가능하지는 않기 때문이다. 따라서 현존하는 암호화 체계보다 더 확실한 안전을 보장해주는 새로운 암호화 체계와 암호화키의 분배시스템의 필요성이 대두되고 있다. (중략)

• Proceedings of the Korea Information Processing Society Conference
• /
• 2003.05c
• /
• pp.1909-1912
• /
• 2003

공개키 암호화와 X.400 프로토콜 그리고 PGP상에 존재하는 메시지 인증 문제를 해결하기 위하여 NMAP로 명명된 인증된 실체기반 암호화 시스템을 제안하고 이를 선계 구현하였다. 제안된 시스템은 전자상거래의 활성화와 비대화형 인증 서비스 제공에 사용될 수 있을 것이다.

• Biomolecules & Therapeutics
• /
• v.24 no.2
• /
• pp.199-205
• /
• 2016

This study aimed to investigate the in vivo relevance of P-glycoprotein (P-gp) in the pharmacokinetics and adverse effect of phenformin. To investigate the involvement of P-gp in the transport of phenformin, a bi-directional transport of phenformin was carried out in LLC-PK1 cells overexpressing P-gp, LLC-PK1-Pgp. Basal to apical transport of phenformin was 3.9-fold greater than apical to basal transport and became saturated with increasing phenformin concentration ($2-75{\mu}M$) in LLC-PK1-Pgp, suggesting the involvement of P-gp in phenformin transport. Intrinsic clearance mediated by P-gp was $1.9{\mu}L/min$ while passive diffusion clearance was $0.31{\mu}L/min$. Thus, P-gp contributed more to phenformin transport than passive diffusion. To investigate the contribution of P-gp on the pharmacokinetics and adverse effect of phenformin, the effects of verapamil, a P-gp inhibitor, on the pharmacokinetics of phenformin were also examined in rats. The plasma concentrations of phenformin were increased following oral administration of phenformin and intravenous verapamil infusion compared with those administerd phenformin alone. Pharmacokinetic parameters such as $C_{max}$ and AUC of phenformin increased and CL/F and Vss/F decreased as a consequence of verapamil treatment. These results suggested that P-gp blockade by verapamil may decrease the phenformin disposition and increase plasma phenformin concentrations. P-gp inhibition by verapamil treatment also increased plasma lactate concentration, which is a crucial adverse event of phenformin. In conclusion, P-gp may play an important role in phenformin transport process and, therefore, contribute to the modulation of pharmacokinetics of phenformin and onset of plasma lactate level.

• Journal of Life Science
• /
• v.18 no.9
• /
• pp.1202-1206
• /
• 2008

The present investigation demonstrates HLA-DR and PGP9.5 double positive cells accumulate thymus cortical region in normal baboon thymus and baboon lung. But, these cells disappeared in thymus and lung of bronchopulmonary dysplasia (BPD) animals. 125d GC animal model is more suitable for BPD than 140d GC animal. Anti-bombesin antibody, 2A11 treated baboon recover normal level of HLA-DR positive cells from BPD animal. In addition, thymocytes show responsiveness for bombesin. These observation suggest that blocking BLPs protects a chronic lung injury by BPD and 2A11 is possible agent for passive therapy of BPD.

• KSBB Journal
• /
• v.31 no.3
• /
• pp.158-164
• /
• 2016

Plant growth promoting (PGP) hormones, which are produced in a small quantity by bacteria, affect in plant growth and development. PGPs play an important role on the crop productivity in agricultural field. In this study, a photosynthetic bacterial strain producing the PGP was isolated from paddy soil. Bacterial isolate was gram negative, rod-shaped and motility positive. From the 16s rRNA gene sequence analysis, the isolate was identified as Rhodobacter capsulatus PS-2. The mass cultivation of R. capsulatus PS-2 was optimized by considering of the carbon, nitrogen and inorganic salt sources. Optimal medium composition was determined as Na-succinate 4.5 g, yeast extract 5 g, $K_2HPO_4$ 1 g, $MgSO_4$ 5 g, per liter. From the result of 500 L fermentation for 2 days using the optimal medium, the viable cells were $8.7{\times}10^9cfu/mL$. R. capsulatus PS-2 strain produced the carotenoid and indole-3-acetic acid (IAA). The carotenoid extraction and quantitative analysis were performed by HCl-assisting method. Total carotenoid contents from R. capsulatus PS-2 culture broth were measured as $7.02{\pm}0.04$ and $6.93{\pm}0.05mg/L$ under photoheterotrophic and chemoheterotrophic conditions, respectively. To measure the productivity of IAA, colorimetric method was employed using Salkowski reagent at optical density 535 nm. The results showed that the highest content of IAA was $197.44{\pm}5.92mg/L$ in the optimal medium supplemented with 0.3% tryptophan.

• Korean Journal of Veterinary Research
• /
• v.53 no.1
• /
• pp.55-60
• /
• 2013

The vomeronasal organ (VNO) plays an important role in reproduction and social activities in ruminants including goats. A morphological study on the structure of VNO and its epithelial cells was carried out in Korean black goats. Grossly, the VNO of Korean goats opens into mouth through incisive ducts. Microscopically, the epithelium of VNO consisted of medial sensory epithelium and lateral non-sensory epithelium. Several blood vessels and nerve bundles were observed in the lamina propria encased by vomeronasal cartilage. Immunohistochemical staining showed that protein gene product (PGP) 9.5 was immunostained in the receptor cells of the sensory epithelium and in some cells of the non-sensory epithelium. Galectin-3 was mainly observed in the supporting cells of sensory and non-sensory epithelium. Lectins including wheat germ agglutinin, Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin Isolectin B4, Dolichos biflorus agglutinin and soybean agglutinin used in this study were bound in VNO sensory, non-sensory epithelia as well as in the lamina propria with varying intensity. Collectively, this is a first descriptive morphological study of VNO of Korean black goat with special reference to lectin histochemistry.

• Journal of Ginseng Research
• /
• v.44 no.2
• /
• pp.321-331
• /
• 2020

Background: The patatin-related phospholipase AIII family (pPLAIIIs) genes alter cell elongation and cell wall composition in Arabidopsis and rice plant, suggesting diverse commercial purposes of the economically important medicinal ginseng plant. Herein, we show the functional characterization of a ginseng pPLAIII gene for the first time and discuss its potential applications. Methods: pPLAIIIs were identified from ginseng expressed sequence tag clones and further confirmed by search against ginseng database and polymerase chain reaction. A clone showing the highest homology with pPLAIIIβ was shown to be overexpressed in Arabidopsis using Agrobacterium. Quantitative polymerase chain reaction was performed to analyze ginseng pPLAIIIβ expression. Phenotypes were observed using a low-vacuum scanning electron microscope. Lignin was stained using phloroglucinol and quantified using acetyl bromide. Results: The PgpPLAIIIβ transcripts were observed in all organs of 2-year-old ginseng. Overexpression of ginseng pPLAIIIβ (PgpPLAIIIβ-OE) in Arabidopsis resulted in small and stunted plants. It shortened the trichomes and decreased trichome number, indicating defects in cell polarity. Furthermore, OE lines exhibited enlarged seeds with less number per silique. The YUCCA9 gene was downregulated in the OE lines, which is reported to be associated with lignification. Accordingly, lignin was stained less in the OE lines, and the expression of two transcription factors related to lignin biosynthesis was also decreased significantly. Conclusion: Overexpression of pPLAIIIβ retarded cell elongation in all the tested organs except seeds, which were longer and thicker than those of the controls. Shorter root length is related to auxinresponsive genes, and its stunted phenotype showed decreased lignin content.

• The KIPS Transactions:PartC
• /
• v.13C no.4 s.107
• /
• pp.435-440
• /
• 2006

XML encryption is to provide confidentiality service, though not limited to, for web contents. XML encryption can be applied to entire data files as opaque objects, or more frequently to various parts of XML documents, supporting various encryption granularity. It is this characteristic that makes XML encryption a more efficient alternative for data confidentiality in various web applications than is possible with SSL/TLS, IPsec, PGP, or S/MIME. It is essential for successful deployment of XML encryption to achieve interoperability among the products implementing this technology, which requires the products to implement the XML encryption standards correctly. Conformance testing is to test if products implement the relevant standard correctly. In this paper we present a conformance testing method for XML encryption products and implement it. We will first look at XML encryption standards developed by W3C, and extract test criteria. Then we propose a testing method in which the encryption capability and the decryption capability of a product are tested separately. The proposed methody is actually implemented as a GUI-based testing tool and some test results are presented.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.1
• /
• pp.66-78
• /
• 2019

In this study, strain KNU17Pc1 was tested for its antifungal activity against Rhizoctonia solani AG-1(IA), which causes banded leaf and sheath blight (BLSB) of maize. KNU17Pc1 was tested further for its broad-spectrum antifungal activity and in vitro plant growth promoting (PGP) traits. In addition, the in vivo effects of KNU17Pc1 on reduction of BLSB severity and seedling growth promotion of two maize cultivars under greenhouse conditions were investigated. On the basis of multilocus sequence analysis (MLSA), KNU17Pc1 was confirmed as P. chlororaphis subsp. aurantiaca. The study revealed that KNU17Pc1 had strong in vitro antifungal activity and was effective toward all in vitro PGP traits except phosphate solubilization. In this study, for the first time, a strain of P. chlororaphis against Colletotrichum dematium, Colletotrichum gloeosporioides, Fusarium oxysporum f.sp. melonis, Fusarium subglutinans and Stemphylium lycopersici has been reported. Further biochemical studies showed that KNU17Pc1 was able to produce both types of phenazine derivatives, PCA and 2-OH-PCA. In addition, solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) analysis identified 13 volatile organic compounds (VOCs) in the TSB culture of KNU17Pc1, 1-undecene being the most abundant volatile. Moreover, for the first time, Octamethylcyclotetrasiloxan (D4), dimethyl disulfide, 2-methyl-1,3-butadiene and 1-undecene were detected in P. chlororaphis. Furthermore, this study reported for the first time the effectiveness of P. chlororaphis to control BLSB of maize. Hence, further studies are necessary to test the effectiveness of KNU17Pc1 under different environmental conditions so that it can be exploited further for biocontrol and plant growth promotion.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.17 no.5
• /
• pp.27-38
• /
• 2007

One of the most important security issues of e-mail service is user privacy. Currently, various security protocols, like PGP(pretty Good Privacy), S/MIME(Secure/Multipurpose Internet Mail Extension), have been proposed. These protocols, however, do not provide forward secrecy. Recently, some security protocols that provide forward secrecy were proposed. But all of them require changes to the current e-mail infrastructure. Moreover, contrary to authors' intention, some of them do not actually provide perfect forward secrecy. In this paper, we propose a new practical e-mail security protocol. The proposed protocol provides perfect forward secrecy and uses a practical e-mail model that dose not require any changes to existing e-mail servers. It encrypts and authenticates messages efficiently using elliptic curve based signcryption scheme. In addition, we provide a way to send secure group e-mails.