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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Mass Cultivation and Secondary Metabolite Analysis of Rhodobacter capsulatus PS-2

광합성세균 Rhodobater capsulatus PS-2의 대량배양 최적화 및 대사산물 분석

  • Bong, Ki Moon (Bio Control Research Institute, Jeonnam Bioindustry Foundation) ;
  • Kim, Jong Min (Bio Control Research Institute, Jeonnam Bioindustry Foundation) ;
  • Yoo, Jae-Hong (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Park, In Chul (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Chul Won (Department of Chemistry, Chonnam National University) ;
  • Kim, Pyoung Il (Bio Control Research Institute, Jeonnam Bioindustry Foundation)
  • 봉기문 (전남생물산업진흥원 생물방제연구센터) ;
  • 김종민 (전남생물산업진흥원 생물방제연구센터) ;
  • 유재홍 (국립농업과학원 농업생물부 농업미생물과) ;
  • 박인철 (국립농업과학원 농업생물부 농업미생물과) ;
  • 이철원 (전남대학교 자연과학대학 화학과) ;
  • 김평일 (전남생물산업진흥원 생물방제연구센터)
  • Received : 2016.08.08
  • Accepted : 2016.09.21
  • Published : 2016.09.30
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Abstract

Plant growth promoting (PGP) hormones, which are produced in a small quantity by bacteria, affect in plant growth and development. PGPs play an important role on the crop productivity in agricultural field. In this study, a photosynthetic bacterial strain producing the PGP was isolated from paddy soil. Bacterial isolate was gram negative, rod-shaped and motility positive. From the 16s rRNA gene sequence analysis, the isolate was identified as Rhodobacter capsulatus PS-2. The mass cultivation of R. capsulatus PS-2 was optimized by considering of the carbon, nitrogen and inorganic salt sources. Optimal medium composition was determined as Na-succinate 4.5 g, yeast extract 5 g, $K_2HPO_4$ 1 g, $MgSO_4$ 5 g, per liter. From the result of 500 L fermentation for 2 days using the optimal medium, the viable cells were $8.7{\times}10^9cfu/mL$. R. capsulatus PS-2 strain produced the carotenoid and indole-3-acetic acid (IAA). The carotenoid extraction and quantitative analysis were performed by HCl-assisting method. Total carotenoid contents from R. capsulatus PS-2 culture broth were measured as $7.02{\pm}0.04$ and $6.93{\pm}0.05mg/L$ under photoheterotrophic and chemoheterotrophic conditions, respectively. To measure the productivity of IAA, colorimetric method was employed using Salkowski reagent at optical density 535 nm. The results showed that the highest content of IAA was $197.44{\pm}5.92mg/L$ in the optimal medium supplemented with 0.3% tryptophan.

Keywords

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