• Title/Summary/Keyword: PGM

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Purification and reaction pattern of cephalexin synthesizing enzyme from Acetobacter turbidans

  • Kang, Sang-Moo;Kim, June-Hoe;Kim, Deog-Jung;Kim, Young-Jun
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.525.2-525
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    • 1986
  • Cephalexin synthesizing enzyme (${\alpha}$ amino acid ester hydrolase) was partially purified from the culture broth of Acetobacter turbidans ATCC9325 through ammonium sulfate fractionation, DEAE, CM, and Sephacryl S-200 gel filtration. The enzyme has optimum pH 6.0 and temperature, 40$^{\circ}C$ respectively. From the analysis of reaction mixtures by thin layer chromatographic and high performance liquid chromatographic techniques, it was confirmed this enzyme catalyzed simultaneously the following reactions : 1) Synthesis of cephalexin from D-${\alpha}$-phenylglycine methylester (PGM) and 7-amino 3-deacetoxy-cetoxycephalosporanic acid (7-ADCA) 2) Hydrolysis of cephalexin to form 7-ADCA and phenylglycine (PG) 3) Hydrolysis of PGM to form PG and methanol. Base on the above experimental observations, the reaction model of this enzyme was identical with that of the enzyme from Xanthomonas citri.

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Electrophoretic Study of Semisulcospira gottschei in Korea (한국산(韓國産) Semisulcospira gottschei의 전기영동적(電氣泳動的) 연구(硏究))

  • Kim, Chang Han
    • The Korean Journal of Malacology
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    • v.2 no.1
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    • pp.30-34
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    • 1986
  • Genetic variations of Semisulcospjra gottschei in Korea were investigated by means of starch gel-electrophoresis. The results are as follows; 1) Eight loci of ${\alpha}$-Gpd, Mdh-2, Pept-1, Pgm-2, Gp-1, Gp-2, Gp-3 and Gp-4 were monomorphic, and 8 loci of Mpi, Mdh-1, Pgi, Got, Pept-2, Pept-3, Pgm-1 and Sdh showed genetic variations. 2) Means of allele per locus ($\bar{A}$) and polymorphism (P) were revealed 0.64 and 0.44, respectively. The value of heterozygosity ($H_G$) showed a little more than heterozygosity $H_D$. ($H_D$=0.09, $H_G$=0.13) 3) Genetic variation appears to be due to loci of Got, Pept, Pgi, and Sdh, all of which of the $H_D$ values are high.

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Allozyme Variability in Tree of Genus Semisulcospira(Prosobranchia: Pleuroceridae) (다슬기속 3종(Prosobranchia: Pleuroceridae)에서의 도위효소 변이)

  • 정영헌;박준우;정평림;박갑만;김재진;민득영
    • The Korean Journal of Malacology
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    • v.15 no.1
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    • pp.13-20
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    • 1999
  • A horizontal starch gel electrophoresis for enzyme proteins extracted from three Korean species and one Chinese species of Semisulcospira was carried out in order to elucidate their genetic relationships. A total of 10 enzymes were employed in three different of buffer systems. Two loci from each enzyme of GAPDH, GOT, ICDH, IDH and PEP(VL); three loci from each of three enzymes, EST, PEP(LGG) and PGDH; and five loci from GPI were observed. Most of the loci in three pleurocerid species employed showed homozygous monomorphic banding patterns and some of them were specific as genetic markers between two different species. However, EST-2, PEP(LGG-3) and PGDH-1 loci in Korean S. libertina and PEP(LGG-3), PGM-1 and PGM-2 loci in Chinese S. libertina showed polymorphic banding patterns. Three Korean Semisulcospira species including S. libertina were more closely clustered in a dendrogram within the range of genetic identity values of 0.818-0.936, and these clusters were lineated with Chinese S. libertina at the value of 0.621.

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Consecutive-Frame Super-Resolution considering Moving Object Region

  • Cho, Sung Min;Jeong, Woo Jin;Jang, Kyung Hyun;Choi, Byung In;Moon, Young Shik
    • Journal of the Korea Society of Computer and Information
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    • v.22 no.3
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    • pp.45-51
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    • 2017
  • In this paper, we propose a consecutive-frame super-resolution method to tackle a moving object problem. The super-resolution is a method restoring a high resolution image from a low resolution image. The super-resolution is classified into two types, briefly, single-frame super-resolution and consecutive-frame super-resolution. Typically, the consecutive-frame super-resolution recovers a better than the single-frame super-resolution, because it use more information from consecutive frames. However, the consecutive-frame super-resolution failed to recover the moving object. Therefore, we proposed an improved method via moving object detection. Experimental results showed that the proposed method restored both the moving object and the background properly.

A Temperature Analysis Study of Korea and its Neighbor Regions for Temperature Specification (온도규격 설정을 위한 한반도 및 주변권역 온도분석 연구)

  • Kim, In-Soo;Kang, Chee-Woo
    • Journal of the Korea Institute of Military Science and Technology
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    • v.18 no.1
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    • pp.55-65
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    • 2015
  • This paper describes the results of a temperature analysis of Korea and its neighbor regions for temperature specification of weapon systems to be deployed in Korea, and introduces the concept of a standard deviation frequency of occurrence which represents a frequency of yearly occurrence. On the basis of this analysis, reasonable operational temperatures for the Korea weapon systems are recommended, and the regional frequency of yearly occurrence of temperatures worse than recommended operational temperatures in each country regions are presented.

Analyzing the Modern Warfare and Weapon Systems Supported by Improved GPS Informations

  • Ko, Kwang-Soob
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.21 no.3
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    • pp.234-239
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    • 2015
  • This paper focuses on analyzing the modern warfare and weapon systems supported by improved GPS informations. The GPS capability was investigated through the real experimental test for verifying the most recent GPS features under its modernization processing. And then it was verified that such capabilities, accuracy and availability, of a typical L1, C/A code GPS receiver are equivalent to the military receiver's ones. It was also sure that the influence of GPS improved informations on NCW(Network-Centric Warfare), PGM(Precision Guided Munition) and C4SIR(Command, Control, Communications, Computers, Intelligence, Surveillance and Reconnaissance) should be increased and the modern warfare may be strongly dependent on GNSS informations.

Study of the requirements measurement program for estimation of requirements (요구사항 평가를 위한 요구사항 품질 척도 프로그램 연구)

  • Lee, Jaung Hun;Kim, Sang Cheon;Kim, Sung Ick;Hwang, Ho Sung
    • Journal of the Korean Society of Systems Engineering
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    • v.4 no.1
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    • pp.1-9
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    • 2008
  • The developer and user of weapon system must define the requirements of weapon system for the satisfaction of stakeholders and complexity of modern weapons in the developing process of weapon system. The perfect requirements reduce risks of the developing system in the initial step. It is not only economy of time and money but also good for the project of developing system. But, the perfect requirement is very difficult because of contradictions between the user and the developer, and limited conditions. So, the structured method and the process is needed for developing requirements.

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Radar Signal Processor Design Using FPGA (FPGA를 이용한 레이더 신호처리 설계)

  • Ha, Changhun;Kwon, Bojun;Lee, Mangyu
    • Journal of the Korea Institute of Military Science and Technology
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    • v.20 no.4
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    • pp.482-490
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    • 2017
  • The radar signal processing procedure is divided into the pre-processing such as frequency down converting, down sampling, pulse compression, and etc, and the post-processing such as doppler filtering, extracting target information, detecting, tracking, and etc. The former is generally designed using FPGA because the procedure is relatively simple even though there are large amounts of ADC data to organize very quickly. On the other hand, in general, the latter is parallel processed by multiple DSPs because of complexity, flexibility and real-time processing. This paper presents the radar signal processor design using FPGA which includes not only the pre-processing but also the post-processing such as doppler filtering, bore-sight error, NCI(Non-Coherent Integration), CFAR(Constant False Alarm Rate) and etc.

A Design of Sensor Simulator for Interoperability between Simulation and Real World Environment (시뮬레이션 및 실 환경에서 상호 운용이 용이한 센서 시뮬레이터 설계)

  • Shim, Jun-Yong;Wi, Soung-Hyouk
    • Proceedings of the Korea Information Processing Society Conference
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    • 2019.10a
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    • pp.708-709
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    • 2019
  • 최근 국방 분야는 모델링 및 시뮬레이션 기술을 활용한 시뮬레이터 개발 사업을 늘리고 있으며, 특히 실 환경에서 시험하기 어려운 다양한 기능과 성능을 시험하기 위해 무기체계 시뮬레이터와 실 장비를 상호 운용할 수 있는 방안을 마련하고 있다. 본 논문은 함정에 탑재되는 대공유도무기체계 개발에 필요한 시뮬레이션 환경을 구축하는데 있어 시뮬레이션과 실환경을 상호 운용할 수 있는 시뮬레이터 설계 방법을 다루며, 특히 소프트웨어로 구현된 다양한 센서 모델과 실 환경에서 운용되는 레이더 및 표적기의 통합 운용이 용이한 센서 시뮬레이터의 구조를 보여준다.

Identification of Loblolly Pine (Pinus taeda L.) Clones through Isozyme Analysis (동위효소분석(同位酵素分析)에 의(依)한 테다소나무(Pinus taeda L.) 클론의 식별(識別))

  • Ryu, Jang Bal;Na, Chun Soo
    • Journal of Korean Society of Forest Science
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    • v.76 no.4
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    • pp.330-337
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    • 1987
  • Megagametophyte tissues from seeds of 45 loblolly pine clones were subjected to horizontal starch-gel electrophoresis. The resulting gels were tested for activity of five enzyme systems (GOT, SDH, PGM, MDH, and AP). Isozymes observed were under control of 11 loci. All 45 clones could be identified with unique genotypes at above 10 loci.

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