• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.10a
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• pp.184-191
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• 2000

In this research, a numerical model is developed for analysis of buried pipelines considering longitudinal permanent ground deformation(PGD) due to liquefaction induced lateral spreading. Buried pipelines and surrounding soil are medeled as continuous pipelines using the beam elements and a series of elasto-plastic springs uniformly distributed along the pipelines, respectively. Idealized various PGD patte군 based on the observation of PGD are used as a loading configuration and the length of the lateral spread zone is considered as a loading parameter. Numerical results are verified with other research results and efficient applicability of developed procedure is shown. Analyses are performed by varying different parameters such as PGD pattern, pipe diameter and pipe thickness. Results show that response of buried pipelines are more affected by pipe thickness than pipe diameter. Finally, the critical length of the lateral spread zone and the critical magnitude of PGD which cause yielding, local buckling or tension failure are proposed for the steel pipe which are normally used in Korea.

• Journal of the Earthquake Engineering Society of Korea
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• v.6 no.2
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• pp.51-61
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• 2002

In this research, a numerical algorithm is developed for the response analysis of burined pipelines considering longitudinal permanent ground deformation(PGD) due to liquefaction induced lateral spreading. Buried pipelines and surrounding soil are modeled as continuous pipelines using the beam elements and a series of elasto-plastic springs represented for equivalent soil stiffness, respectively. Idealized various PGD patterns based on the observation of PGD are used as a loading configuration and the length of the lateral spread zone is considered as loading parameter. Numerical results are verified with other research results and efficient applicability of developed procedure is shown. Analyses are performed by varying different parameters such as PGD pattern, pipe diameter and pipe thickness. Through these procedures, relative influences of various parameters on the response of buried pipeline subject to longitudinal PGD are investigated.

• Journal of the Earthquake Engineering Society of Korea
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• v.7 no.5
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• pp.37-46
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• 2003

Response analysis of buried pipeline subjected to permanent ground deformation(PGD) due to liquefaction is mainly executed by use of numerical analysis or semi-analytical relationship, Especially for the semi-analytical relationship considering transverse PGD, it has somewhat limited applicability : since it has different formula according to the width of PGD and does not reflect various patterns of PGD which is caused by the decrease of soil stiffness, Therefore, in this study, the applicability of existing analytical relationship is closely investigated through the comparison of FEM results at first. And then, based on meaningful contemplation, improved analytical relationship is proposed. The proposed one models the system behavior of buried pipeline as the combination of cable and beam, and thus it is applicable to arbitrary width of PGD, Moreover, it does reflect various patterns of PGD by introducing interaction pattern coefficient. Through the comparison of numerical results using the FEM and the proposed analytical relationship, rational applicability is objectively verified and noticeable considerations are discussed, Moreover, analyses considering the change of PGD magnitude and patterns are performed.

• Clinical and Experimental Reproductive Medicine
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• v.26 no.1
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• pp.9-20
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• 1999

The genetic defects in human gametes and embryos can cause adverse effects on overall reproductive events. Biopsy of embryos for preimplantation genetic diagnosis (PGD) offers a new possibility of having children free of the genetic disease. In addition, advanced embryo culture method may enhance the effectiveness of embryo biopsy for the practical application of PGD. This experimental study was undertaken to evaluate the effects of coculture on the development in vitro of biopsied mouse embryos as a preclinical model for PGD of human embryos. Embryos were obtained after in vitro fertilization (IVF) from F1 hybrid mice (C57BLfemale/CBAmale). Using micromanipulation, 1, 2, 3 or 4 blastomeres of 8-cell stage embryos were aspirated through a hole made in the zona pellucida by zona drilling (ZD) with acidic Tyrode's solution (ATS). After biopsy of blastomeres, embryos were cultured in vitro for 110 hours in Ham's F-10 supplemented with 0.4% BSA or cocultured on the monolayer of Vero cells in the same medium. The frequence of blastocyst formation were recorded, and the embryos beyond blastocyst stage were stained with 10% Giemsa to count the total number of nuclei in each embryo. There was no significant difference in the blastocyst formation between the zona intact control group and the zona drilling (ZD) only, or biopsied groups. The hatching rate of all the treatment groups except 4/8 group was significantly higher than that of control group. In all the treatment groups, there was a significant reduction in the mean cell number of embryos beyond blastocyst stage ($50.2{\pm}14.0$ in control group vs. $41.2{\pm}7.9$ in ZD, $39.3{\pm}8.8$ in 7/8, $29.7{\pm}6.4$ in 6/8, $25.1{\pm}5.7$ in 5/8, and $22.1{\pm}4.3$ in 4/8 groups, p<0.05). When the same treatments were followed by coculture with Vero cells, a similar pattern was seen in the blastocyst formation and the hatching rate. However, in all the treatment groups, there was a significant increase in the mean cell number of embryos beyond blastocyst stage with coculture, compared with the parallel groups without coculture. In the cleavage rate of biopsied blastomeres cultured for 110 hours after IVF, there was no significant difference between coculture and non-coculture groups (87.2% vs. 78.7%). However, the mean cell number of embryos developed from the biopsied blastomeres was significantly higher in coculture group ($11.5{\pm}4.7\;vs.\;5.9{\pm}1.9$, p<0.05). In conclusion, biopsy of mouse embryos after ZD with ATS is a safe and highly efficient method for PGD, and coculture with Vero cells showed a positive effect on the development in vitro of biopsied mouse embryos and blastomeres as a preclinical model for PGD of human embryos.

• Animal Systematics, Evolution and Diversity
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• v.5 no.1
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• pp.77-88
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• 1989

Genetic and morphometric comparison between two species of the genus Agkistrodon were performed to estimate the degree of genic variation and to clarify the taxonomic status of Cheju population, a suspected hybrid form between A. blomhoffii brevicaudus and A u ussuriensis. A sum of 147 specimens representing six populations of A.b. brevicaudus and two populations of A ussuriensis was used in this study. Out of the 21 loci examined, 5 loci (Adh, Ldh-2, Mdh-2, Mpi, Pgi) were monomorphic with identical mobility in both species. O Other Sioci (Aco, Gp, Fum, [po and Xdh in A b. brevicaudus; Est, Got-l, Gp, Mdh-l and 6Pgd in A ussuriensis) were highly poplymorphic. The degree of genic variation of Ab. b brevicaudus and A ussuriensis was A=1.SS, P=42.1%, HD=0.096, HG=O.l1S, and A= 1.4S, P = 31.6%, HD=0.117, HG =0.121, repectively. These values are twofold higher t than those of other reptilian species reported (Selander, 1976) including Korean species of Rhabdophis tigrina and Elaphe dione (Paik and Yang, 1986, 1987). The average values of t the genetic similarities among six populations of A b. brevicaudus and two populations of A ussuriensis were S=0.919 and S=0.962, respectively, whereas the value between species was S = 0.662. Presumed divergent time estimate (Nei, 1975) of these two species was about 1 1.8 million years ago. The analysis of the geographic variations of various morphological c characters was based on Gloyd’s criteria (Gloyd, 1972). Coloration of tongue and tail tip, t the number of ventral scales, and the number of subcaudal scales are good diagnostic c characters to identify these two species. The pattern of cross band is, however, highly variable within and between populations of both species. No hybrid was detected and the s suspected hybrid form of Cheju population falls within the range of A. ussuriensis in the g genetic and morphometric analyses.