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Evaluation of Embolization Effect of Hepatocellular Carcinoma by Hepatic Arterial Flow Study with $^{99m}Tc$-MAA SPECT (간암에서 색전술의 효과를 평가하는데 있어서 $^{99m}Tc$-MAA SPECT를 이용한 간동맥 혈류 검사의 의의)

  • Lee, Byung-Hee;Yoo, Hyung-Sik;Lee, Jong-Doo;Chung, Jin-Ill;Park, Chang-Yun;Lee, Jong-Tae
    • The Korean Journal of Nuclear Medicine
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    • v.28 no.1
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    • pp.62-68
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    • 1994
  • This study was aimed to compare the density of the functional microcirculation of hepatocellular carcinoma (HCC) with normal liver and to investigate the effect of hepatic-arterial oily chemoembolization (HAE) by radionuclide examination. Methods : Eight patients with HCC proven by biopsy in five, and clinically and radiologically in three were included. The mixture of 2 cc normal saline with three to four mCi of $^{99m}Tc$-MAA was infused through a hepatic-arterial catheter for a minute. Dynamic images were obtained at a rate of 4 sec per frame for a minute, and static images and SPECT were followed. Results : In three patients who underwent hepatic arterial angiography (HAA) alone, radioactivity was markedly increased in tumors compared to the adjacent liver immediately after infusion of $^{99m}Tc$-MAA. The ratios of tumoral and extratumoral up-take (T/E ratio) were above 6.5 (range; $6.5{\sim}l9$, mean; 12.5). In four of the five patients who under-went superselective HAE, T/E ratio were remark-ably decreased ($0.5{\sim}1.3$). The areas of embolization were better delineated in radionuclide study than in postembolization HAA. In the other one who was considered to be embolized completely on HAA, strong radiouptake in the tumor was disclosed (T/E ratio; 7.0). Conclusions : Therefore hepatic-arterial flow study with radionuclide imaging using $^{99m}Tc$-MAA can be a valuable method to assess the accurate embolization effect in HCC.

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Protoplast Formation, Regeneration and Reversion in Pleurotus ostreatus and P. sajor-caju (느타리버섯과 여름느타리버섯의 원형질체(原形質體) 나출(裸出)과 재생(再生))

  • Go, Seung-Joo;Shin, Gwan-Chull;Yoo, Young-Bok
    • The Korean Journal of Mycology
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    • v.13 no.3
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    • pp.169-177
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    • 1985
  • The studies were carried out to obtain the basic data for maximizing the protoplast yields from the mycelia of P. ostreatus and P. sajor-caju. Some factors affecting the regeneration of the protoplast of both species and the productivity of their reversion were also examined. The maximum yields of protoplasts were obtained from four days cultured mycelia of both species on cellophan membrane placed on the surface of PSA or MCM media in a petri dish. The optimal concentration of lytic enzyme Novozym 234 for protoplast releasing was 5 mg per ml of 0.5 M phosphate buffer solution with 0.6 M sucrose or 0.6 M $MgSO_4$ at pH 6.0. The greatest number of protoplasts was released 3 hours after incubation of the mycelia of P. ostreatus and after 4 hours for the P. sajor-caju in the lytic enzyme solution. Among the osmotic stabilizer solutions tested 0.6 M sucrose and 0.6 M KCl showed the best regeneration rates of the protoplasts of both species. When 0.75 % agar solution was over-layed on the regeneration media immediately after inoculation of the protoplast the regeneration rates were greatly enhanced. The ampicillin added to the agar solution prevented bacteria from infection. The reverted isolates produced the sporophores and basidial spores just like their parents without any mutations when they were cultivated in a broad mouth bottle with sawdust substrates.

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Changes in Radiation Use Efficiency of Rice Canopies under Different Nitrogen Nutrition Status (질소영양 상태에 따른 벼 군락의 광 이용효율 변화)

  • Lee Dong-Yun;Kim Min-Ho;Lee Kyu-Jong;Lee Byun-Woo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.8 no.3
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    • pp.190-198
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    • 2006
  • Radiation use efficiency (RUE), the amount of biomass produced per unit intercepted photosynthetically active radiation (PAR), constitutes a main part of crop growth simulation models. The objective of the present study was to evaluate the variation of RUE of rice plants under various nitrogen nutritive conditions. from 1998 to 2000, shoot dry weight (DW), intercepted PAR of rice canopies, and nitrogen nutritive status were measured in various nitrogen fertilization regimes using japonica and Tongil-type varieties. These data were used for estimating the average RUEs before heading and the relationship between RUE and the nitrogen nutritive status. The canopy extinction coefficient (K) increased with the growth of rice until maximum tillering stage and maintained constant at about 0.4 from maximum tillering to heading stage, rapidly increasing again after heading stage. The DW growth revealed significant linear correlation with the cumulative PAR interception of the canopy, enabling the estimation of the average RUE before heading with the slopes of the regression lines. Average RUE tended to increase with the increased level of nitrogen fertilization. RUE increased approaching maximum as the nitrogen nutrition index (NNI) calculated by the ratio of actual shoot N concentration to the critical N concentration for the maximum growth at any growth stage and the specific leaf nitrogen $(SLN;\;g/m^2\;leaf\;area)$ increased. This relationship between RUE (g/MJ of PAR) and N nutritive status was expressed well by the following exponential functions: $$RUE=3.13\{1-exp(-4.33NNNI+1.26)\}$$ $$RUE=3.17\{1-exp(-1.33SLN+0.04)\}$$ The above equations explained, respectively, about 80% and 75% of the average RUE variation due to varying nitrogen nutritive status of rice plants. However, these equations would have some limitations if incorporated as a component model to simulate the rice growth as they are based on relationships averaged over the entire growth period before heading.

Varietal Response to Grain Quality and Palatability of Cooked Rice Influenced by Different Nitrogen Applications (질소 시비조건에 따른 벼 품종의 미질과 식미특성 반응)

  • Kim, Jeong-Il;Choi, Hae-Chun;Kim, Kwang-Ho;Ahn, Jong-Kuk;Park, No-Bong;Park, Dong-Soo;Kim, Chun-Song;Lee, Ji-Yoon;Kim, Jae-Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.1
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    • pp.13-23
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    • 2009
  • To intensively analyze and examine the changes in palatability of cooked rice and structural properties of rice grains influenced by increased nitrogen topdressing during the panicle formation and ripening stage, a series of experiments were carried out using three japonica rice cultivars with contrasting rice quality characteristics. The protein content of brown rice increased maximally up to $35{\sim}47%$ of that in standard N6 plot with the increase in nitrogen topdressing fertilizer during 20 days before heading to 10 days after flowering. The high-yielding rice cultivars showing poor palatability of cooked rice revealed larger increase in protein content of rice grains by increased nitrogen topdressing. Under the same nitrogen level of 15 kg per 10a with nitrogen topdressing at 3 kg/10a, high-yielding rice cultivars, Yumehikari and Reihou showed the significant increase in protein content of brown rice when topdressing was applied at 10 days after flowering as compared with when it was applied at 30 days after transplanting. Although the variation in amylose content of milled rice as affected by nitrogen topdressing level was relatively small, it decreased within 1% with the opposite tendency against increased protein content of brown rice by increased nitrogen topdressing. The total score of sensory evaluation was higher in the order of Hinohikari < Yumehikari < Reihou in panel test. It decreased significantly by increased amounts of nitrogen topdressing during 20 days before heading to 10 days after flowering when nitrogen level was higher than 12 kg/10a. The more poor palatable rice cultivar in panel test revealed the larger decreasing in total score of sensory evaluation by higher nitrogen topdressing rates. All sensory evaluation components were largely affected by the change in protein content of brown rice rather than amylose content of milled rice. The influence of protein content to palatability of cooked rice was larger in poor-palatable rice than in high-palatable rice. The protein content decreased drastically from outer layer to inner layer of rice grains, while the amylose content increased on the contrary. The high-palatable rice exhibited higher distribution of protein content on bran layer but lower distribution of protein content on the layer of polished rice as compared with the poor-palatable rice. Especially, the high-palatable rice showed also significantly lower distribution of amylose content on the outer layer of polished rice as compared with the poor-palatable rice.

A Program of Water Quality Management for Agricultural Reservoirs by Trophic State (농업용 저수지의 부영양화와 수질관리방안)

  • Lee, Kwang-Sik;Yoon, Kyung-Sup;Kim, Ho-Il;Kim, Hyung-Joong
    • Korean Journal of Environmental Agriculture
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    • v.22 no.2
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    • pp.166-171
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    • 2003
  • A total of 498 agricultural reservoirs ranging from $164{\times}10^3\;m^3$ to $253{\times}10^6\;m^3$ in storage volume were investigated from 1990 to 2001 with respect to Chl-${\alpha}$, COD concentration and pollutant loading of BOD, TN, and TP. The lakes and reservoirs could be classified to 4 types using the relationships between the ratio of storage volume per water surface area(ST/WS) and Chl-${\alpha}$ concentration. It is recommended that the improvement of polluted lakes should be performed in the order of integrated consolidation type ${\rightarrow}$ watershed consolidation type ${\rightarrow}$ in-lake consolidation type ${\rightarrow}$ Management type and reservoir should be constructed to be over $5{\sim}6\;m$ in depth(ST/WS ratio) for preventing the eutrophication of agricultural reservoirs. We propose that water quality criteria for agricultural water is changed from less than 8 mg/L to less than 6 mg/L for safety value, $6{\sim}10\;mg/L$ for concern value, and more than 10 mg/L for countermeasure value in COD concentration, respectively.

Population Structure and Habitat Characteristics of Deutzia paniculata Nakai, as an Endemic Plant Species in Korea (한반도 특산식물 꼬리말발도리 개체군 구조 및 서식지 특성)

  • Jung, Ji-young;Pi, Jung-hun;Park, Jeong-geun;Jeong, Mi-jin;Kim, Eun-hye;Seo, Gang-Uk;Lee, Cheul-ho;Son, Sung-won
    • Korean Journal of Ecology and Environment
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    • v.49 no.1
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    • pp.31-41
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    • 2016
  • Deutzia paniculata is an endemic species to the Korean Peninsula. Despite of importance for conservation, the population structure and habitat characteristics of D. paniculata have not been determined yet. We analyzed the ecological characteristics of the species based on the literature review and field survey. Field survey was conducted on May to October 2014 during which 11 quadrats of size $15{\times}15m$ were studied in six regions. Each of the quadrats were further divided into $5{\times}5m$ small quadrats and population characteristics were recorded. The population and habitat characteristics were analyzed, including species abundance (density and coverage), demographic attributes (flowering rates and fruiting plants), vegetation (structure, species composition), light availability (transmitted light and canopy openness) and soil characteristics (temperature and humidity). We found that D. paniculata mainly distributed in Gyeongsangdo (including Taebaek in Gangwondo) along a broad elevational range of 290~959 m (mean: 493 m) above sea level. In preferred habitat the species grows within the slope range of $7^{\circ}$ and $35^{\circ}$ with the average of $16^{\circ}$. D. paniculata was generally distributed on talus deposits and low adjacent slopes. The average number of individual plants per small quadrat was 12.5 with the mean density $0.5stems\;m^{-2}$. The vegetative reproduction was frequent in D. paniculata and mean flowering rate was as low as 15%. Altogether 138 taxa were found in whole observation area with the dominant tree species mainly spring ephemerals, such as Cornus controversa (importance value: 25.5%) and Fraxinus rhynchophylla (importance value: 15.8%). Although, C. controversa usually grows on steep slopes and F. rhynchophylla mostly distributed at high-altitudes, however, both species distributed in disturbed environments and among talus deposits. Thus based on our results, we concluded that D. paniculata is a disturbance-prone species, primarily existing in habitats subjected to natural disturbances, such as floods. The species occurs less at anthropogenically disturbed sites, thus there is no apparent threat to the populations and habitat of D. paniculata.

Effect of Application of Pig Manure Slurry to Rye on Rice Productivity in Paddy-land (답작지대에서 돈분액비에 의한 호밀 생산시 후작 벼의 생산성에 미치는 영향)

  • Yoon Chang;Yook Wan-Bang;Choi Ki-Choon;Lee Kyung-bo;Chung Ku-Nam
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.4
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    • pp.251-258
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    • 2005
  • This study was conducted to examine the effect of application of pig manure slurry(PMS) for rye cultivation in Paddy-land on rice productivity The field experiments were carried out from 1999 to 2002 on well-grown rice in Paddy-land at Kimje, Chunbuk province in Korea. PMS was manufactured by the fermentation for 6 months in natural condition. Amount of PMS was adjusted equal to total nitrogen value relative to its value of commercial chemical fertilizers. The amount of PMS were spread evenly over the soil surface two times; first in the autumn (before sowing, end of November) and second in the spring(regrowth of rye, middle of March). The field plots were consisted of four kinds (PMS $100\%$ treatment, PMS $200\%$ treatment PMS $100\%$ treatment with half of CF (CF $50\%$), Full of CF treatment (CF $100\%$). The experiment was designed as a randomized complete block. Each treatment was replicated three times. Rices are customarily gown under tropical korean condition as annuals. The results obtained from this study were summarized as fellows. Panicle length of rice was shorter in PMS $100\%$ than other treatments. Ripening grain ratio was the lower in CF treatment. In treatment of PMS $100\%$ and PMS $200\%$, 1000 grain weight was a downward trend as compared to that of full of CF treatments, whereas number of grains per $m^2$ was increased as compared to other treatments. Number of panicles reveals that there is an increase in order; CF $50\% + PMS\;100\%>\;PMS\;100\%\;>\;CF\;100\%\;>\;PMS\;200\%$. Yield of milled rice showed hi인 in CF $100\%$ and $CF 50\%+ PMS 100\%$, but were was no significant difference among treatments. There were did not a regular tendency in the ratio of leaf sheath bright, however the ratio of lodging area was higher in $CF50\%+PMS\;100\%$ treatment than other treatments. Based on the results of this experiment, $CF 50\%+PMS\;100\%$ may be the most effective in rice productivity under application of PMS for rye cultivation in Paddy-land.

Comparison of Non-structural Carbohydrate Concentration Between Zoysiagrass and Creeping Bentgrass During Summer Growing Season (하계 생육기 동안 Zoysiagrass와 Creeping Bentgrass의 비구조적 탄수화물 함량의 비교)

  • Kim, Dae-Hyun;Jung, Woo-Jin;Lee, Bok-Rye;Kim, Kil-Yong;Kim, Tae-Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.2
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    • pp.145-152
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    • 2002
  • To compare the Carbon metabolic response to high temperature stress in Zoysiagrass [Zoysia matrella (L.) Merr.] and Creeping bentgrass (Agrostis palustris Huds) with respect to heat tolerance, C metabolites were determined from April to September. Sampling was carried out on an established golf course (Muan Country Club, Chonnam, Korea). Shoot mass(g Dry weight per hole cup) of creeping bentgrass started to decrease from June and recovered from August whereas that of zoysiagrass was less varied. Chlorophyll content in creeping bentgrass was significantly higher than zoysiagrass until July, and then decreased by 43% from July to August. Zoysiagrass contained higher soluble sugar than creeping bentgrass throughout experimental period. Soluble sugar in zoysiagrass increased about 58% from April to May, and less varied until August. Soluble sugar in creeping bentgrass slightly increased until July and sharply decreased at August. Starch concentration in zoysiagrass continuously decreased to September after a significant increase from April to May. A remarkable fluctuation in both starch and fluctuation concentration was observed between June and August showing high accumulation for June to July and high degradation for July to August. These results suggest that through creeping bentgrass suffers much severely from high temperature stress than zoysiagrass especially June to August. An active accumulation and degradation in nonstructural carbohydrate in creeping bentgrass during this period might be associated with heat stress.

Evaluation of Continuing Education Program to Enhance Competency for Hospice Volunteers: An Exploratory Mixed-Methods Design (호스피스 자원봉사자 역량강화를 위한 지속교육의 효과: 혼합연구방법의 적용)

  • Seo, Minjeong;Cho, Han-A;Han, Sang Mi;Ko, Youngshim;Gil, Cho-Rong
    • Journal of Hospice and Palliative Care
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    • v.22 no.4
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    • pp.185-197
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    • 2019
  • Purpose: Hospice volunteers are serving an invisible yet pivotal role in the hospice and palliative care team. This study investigated how effectively a continuing education program could enhance hospice volunteers' competency. Methods: A total of 20 hours (four hours per week) of training was provided to 30 hospice volunteers who participated in the continuing education for hospice volunteers. Efficiency of the education was analyzed with an exploratory mixed-methods design. For quantitative analysis, the volunteers were asked, before and after the training, about their attitudes towards hospice care, what makes a meaningful life, self-efficacy and satisfaction with their volunteer service. Descriptive statistics, paired t-tests, and Wilcoxon signed-rank test were performed using SPSS Window 20.0. For qualitative research, participants were placed in three groups for a focus group interview, and data were analyzed by content analysis. Results: A quantitative study result shows that this training can significantly affect hospice volunteers' attitudes and improve their self-efficacy. A qualitative study result shows that participants wanted to receive continuous education from the physical/psychosocial/spiritual aspects to better serve end-of-life patients and their family members even though they have to spare significant time for the volunteer service. They wanted to know how to take good care of patients without getting themselves injured and how to provide spiritual care. Conclusion: The continuing education program reflecting volunteers' requests is strongly needed to improve their competency. An effective continuing education requires continuous training and support in areas where hospice volunteers are interested in. A good alternative is to combine web-based and hands-on training, thereby allowing hospice volunteers freely take training that suits their interest.

A study of Establishment on Radiomap that Utilizes the Mobile device Indoor Positioning DB based on Wi-Fi (Wi-Fi 기반 모바일 디바이스 실내측위 DB를 활용한 라디오맵 구축에 관한 연구)

  • Jeong, In Hun;Kim, Chong Mun;Choi, Yun Soo;Kim, Sang Bong;Lee, Yun
    • Journal of Korean Society for Geospatial Information Science
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    • v.22 no.3
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    • pp.57-69
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    • 2014
  • As of 2013, Korean population density is 505 persons per $1km^2$ and is ranked 3rd place in the most densely populated countries exception of city-states. It shows clearly the population is concentrated in the city area. To fulfil this urban concentration population demand, the enlargement and complexation of buildings, subway and other underground spaces connection tendency has been intensified, and it is need to construct the indoor spatial information DB as well as the accurate indoor surveying DB to promote people's safety and social welfare. In this study, Sadang station and Incheon National Airport were aimed for the construction of Wi-Fi AP location DB and RadioMap DB by collecting the indoor AP raw datas by using mobile device and those collected results were ran through the process of verification, supplementation, and analyzation. To evaluate the performance of constructed DB, 10 points in Incheon Airport- 3rd flr in block A, and 9 points in Sadang station-B1 were selected and calculated the estimated points and ran evaluation experiment using survey positioning error, which is distance between real position and the estimated position. The result shows that Incheon international airport's average and standard deviation was separately 17.81m, 17.79m and Sadang station's average and standard deviation was separately 22.64m, 23.74m. In Sadang station's case, the areas near the exit has low performance of surveying position due to fewer visible AP points than other areas. As total datas were examined except those position, it was verified that the user's location was mapping close position in surveying positioning by using constructed DB. It means that constructed DB contains correct Wi-Fi AP locations and radio wave patterns in object region, so it is considered that the indoor spatial information service based on constructed DB would be available.