• Korean Journal of Veterinary Research
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• v.35 no.3
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• pp.515-530
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• 1995

Coronaviridae에 속하는 transmissible gastroenteritis virus(TGEV)와 porcine epidemic diarrhea virus(PEDV)를 specific하게 detection할 수 있는 방법을 개발하고자 본 연구를 수행하였다. 두 바이러스 모두 RNA 바이러스이기 때문에 reverse transcription-polymerase chain reaction(RT-PCR)으로 nucleocapsid(N) protein gene의 cDNA를 증폭시켰다. SmaI으로 처리한 pTZ19R에 ligation시킨 후 염기서열을 밝히고자 sequencing하였다. 각각의 prototype virus와 비교하여 상동성을 밝혔다. 두 바이러스에 대한 cDNA probe를 제작하여 Southern blot hybridization을 실시하였다. TGEV의 경우 백신주인 P45와 병독주인 Miller strain을 사용하였다. cDNA를 증폭시키기 위해 N1/N1R과 N2/N2R 두 가지 primer를 이용한 결과, N1/N1R primer의 경우 586bp 크기의 PCR product를 얻을 수 있었고, N2/N2R primers로 582bp의 cDNA를 증폭시킬 수 있었다. PEDV 실험을 위하여 PED 임상 증상을 나타내는 분변을 이용하여 RT-PCR을 실시하였다. P2/P2R primer로 753bp의 PCR product를 얻을 수 있었다. TGEV의 두 가지 strain의 N protein gene을 sequencing하여 prototype인 Purdue strain과 염기서열 상동성을 조사한 결과, 97%이상의 높은 homology를 나타내었다. PED-V 역시 N protein gene을 sequencing하여 CV777과 염기서열 상동성을 조사한 결과 97%이상의 homology로 PEDV임을 알 수 있었다. TGEV와 PEDV의 염기서열을 비교한 결과 29%의 낮은 homology를 관찰할 수 있었다. 두 가지 바이러스의 N protein gene에 대한 cDNA probe를 제작하여 Southern blot hybridization을 한 결과, 각 바이러스에 매우 특이적 반응을 나타내었다.

• Journal of the Korea Society of Computer and Information
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• v.26 no.4
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• pp.11-19
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• 2021

An abnormal object refers to a person, an object, or a mechanical device that performs abnormal and unusual behavior and needs observation or supervision. In order to detect this through artificial intelligence algorithm without continuous human intervention, a method of observing the specificity of temporal features using optical flow technique is widely used. In this study, an abnormal situation is identified by learning an algorithm that translates an input image frame to an optical flow image using a Generative Adversarial Network (GAN). In particular, we propose a technique that improves the pre-processing process to exclude unnecessary outliers and the post-processing process to increase the accuracy of identification in the test dataset after learning to improve the performance of the model's abnormal behavior identification. UCSD Pedestrian and UMN Unusual Crowd Activity were used as training datasets to detect abnormal behavior. For the proposed method, the frame-level AUC 0.9450 and EER 0.1317 were shown in the UCSD Ped2 dataset, which shows performance improvement compared to the models in the previous studies.

• Korean Journal of Health Psychology
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• v.16 no.3
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• pp.483-500
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• 2011

Since the introductionof modern therapies, more children with cancer have survived their illness. As a result, an interest in the degree of adjustment achieved by cancer survivors has increased. In this study, quality of life and behavior problemsof child and adolescent cancer survivors were compared with those of healthy controls. Also, the patternsof the discrepancy between parent-report and self-report were compared. Childhood cancer survivors aged 8-18 and their mothers were participated in the study. Parent and self report versions of PedQL were administered to assess the quality of life. K-CBCL and K-YSR were further completed to assess internalizing and externalizing behavior problem. Results suggested that cancer survivors generally showed lower quality of life in physical and social domains and more internalizing behavior problem than healthy control group. However, the results were affected by the domain, age group, and informant. The pattern of discrepancy between parent and self report was similar. All parents reported higher levelsof quality of life and lower levels of behavior problems compared to the self reports of their siblings. In assessing internalizing problem behaviors, cancer survivor group showed greater discrepancy than the healthy control group.The implications, limitations, and directions for future research were also discussed.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.10 no.6
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• pp.23-29
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• 2010

Nowadays it is required to design intelligent visual surveillance systems which automatically detect abandoned objects in public places to strengthen the social safety. Already recognized abandoned objects can be occluded partially or fully by surrounding people in public places after the first recognition. To improve an essential recognition performance index PAT, the system should overcome the occlusion problems. In this research, a design scheme is newly proposed to construct the robust detection system which is comprised of multiple stages considering the occlusion problem. To show the feasibilities of the proposed system, the evaluation was tried for the prepared image streams including 6 various situations and the experimental results show 96% and 75% in PAT performance for intrusion and abandoning events, respectively. Finally in spite of full occlusions by multiple persons, the proposed system shows the capability to continuously recognize the abandoned object after complex occlusions disappear.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.6
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• pp.1872-1881
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• 1996

LBB(Leak-Before-Break) analysis is performed for the highest stress location of each different type of mateerials in the nuclear piping line. In most cases, the highest stress occurs in the pipe and nozzle interface location. i.e. terminal end. The current finite element analysis approach utilizes the symmetry condition both for locations near the nozzle and for locationa away from the nozzle to minimize the size of the finite element model and to make analysis simple when calculating the J-integral values at the crack tip. In other words, the nozzle is not included in the finite element model. However, in reality, the symmetric condition is not applicable for the pipe-nozzle interface location. Because the pipe-nozzle interface location is asymmetric due to different stiffenss of the pipe and nozzle(both material and dimensions). The simplified analysis approach for pipe-nozzle interface locaiton is too conservative for a smaller diameter piping. In tlhis paper, various analyses are performed for the range of materials and crack sizes to evaluate the nozzle effect for a LBB anlaysis. This paper presents methodology for developing the piping evaluaiton diagram at the pipe-nozzle interface location.

• Korean Journal of Veterinary Research
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• v.43 no.3
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• pp.477-483
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• 2003

Molecular diagnostic techniques have been used to identify porcine epidemic diarrhea virus (PEDV), a causative agent of acute enteritis in swine, but they were difficult to be petformed and time-consuming. To detect PEDV in a rapid and easy way, we developed biotinylated cDNA probe for N gene encoding the nucleoproteins of PEDV. Formalin-fixed and paraffin-embedded tissues from 24 naturally infected pigs were used for the experiment. The ISH produced a positive reaction in all cases. When intestinal tissues were hybridized with PEDV probe, strong signals were seen in the villus enterocytes of the jejunum and ileum. Hybridization signals were also found in the duodenum from one pig and in colon from dnother. In conclusion, ISH with a biotinylated cDNA probe was provided to be a useful diagnostic method for detecting PEDV effectively in routinely processed tissue sections.

• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1215-1218
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• 2010

The recombinant DNA pLR5cat_PSAB, in which pediocin PA-1 structural and immunity genes (pedAB) fused with the promoter and deduced signal sequence of an ${\alpha}$-amylase gene from a bifidobacterial strain were inserted in Escherichia coli-lactobacilli shuttle vector pLR5cat, was transferred to Lactobacillus reuteri KCTC 3679 and the transformant presented bacteriocin activity. The recombinant L. reuteri KCTC 3679 transformed with the shortened pLR5cat(S)_PSAB, where a nonessential region for the lactobacilli replicon was removed, also showed bacteriocin activity. The molecular mass of the secreted pediocin PA-1 from the recombinant bacteria was the same as that of native pediocin PA-1 (~4.6 kDa) from Pediococcus acidilactici K10 on a sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel. In cocultures with Listeria monocytogenes, the recombinant L. reuteri KCTC 3679 effectively reduced the viable cell count of the pathogenic bacterium by a 3 log scale compared with a control where L. monocytogenes was incubated alone.

• Microbiology and Biotechnology Letters
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• v.46 no.3
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• pp.288-299
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• 2018

Food bio preservation is of major interest in the food industry. Many types of antimicrobial compounds can be produced by lactic acid bacteria (LAB), including bacteriocins. Bacteriocins increase the shelf-life of food by decreasing some food-borne diseases. In this study, a multi-coding sequence of bacteriocin genes was used for primer design to produce bacteriocin genes in Enterococcus faecium AH2 strain and Pediococcus pentosaceus AH1. Multi-coding sequences were aligned to detect conserved sequences in the bacteriocin gene. Eight genes encoding proteins involved in bacteriocin production were isolated and sequenced, including six from E. faecium AH2 (entA, entI, entF, entR, orfA2, orfA3) and two from P. pentoceseus AH1 (papA, pedB), and all gene sequences were deposited in the Gen Bank database under accession numbers LC064146-LC064151, LC101300, and LC101789, respectively. P. pentosaceus AH1 and E. faecium AH2 strains displayed bacteriocin activities of $2610AU\;mL^{-1}$ and $690AU\;mL^{-1}$ and inhibition zones of 26 mm and 19 mm, respectively. Overexpression of entA in E. faecium AH2 increased the bacteriocin and antimicrobial activities.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.4
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• pp.58-71
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• 1989

This study aims to develop a computerized program for analysis of the ground-supported cylindrical shell structure with step varied section and to find out its mechanical characteri- stics through application of the developed program to the analysis of a ensiled farm silo as a model structure. The thickness of wall and bottom-plate of farm silo is assumed to be step-varied and its detailed structural dimensions are presented in Tab. 1 and 2. Several numerical case studies show that sectional stresses of the sample structures are largely reduced by adopting "varied section" design technique. And, other major results ob- tained from this study are summarize4 as follows ; 1. The variation of wall-thickness has a great influence on bending stresses of wall. Ho- wever, the larger the relative thickness of bottom-plate is, the smaller the influence is. 2. The magnitude of thickness of projecting toe of bottom-plate has negligible effect on sectional stresses 3. The conventional design methodology, which assumes the bottom edge of wall as clam- ped on ground, is proved to be discarded through the numerical analysis. 4. It is found that the "varied section" design technique should get similar effects as in the case of thick bott6m-plate having uniform thickness. 5. The variation of wall-thickness has a considerable effect on the bending stresses of bo- ttom-plate. Especially, this phenomenon is very remarkable in its projecting toe. In some cases. the negative bending moment may be acted on.

• Korean Journal of Veterinary Service
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• v.19 no.1
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• pp.46-54
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• 1996

This study was perfomed to examine the distribution of causative agent of piglets diarrhea in eastern Chonnam province from February 1994 to March 1995. The causative agents of diarrhea were examined by bacterial culture test, parasitological test and serological test against PED, TGE and Rota. The 35 isolated E. coli were tested for antibiotic sensitivity. The results obtained were as follows. 1. The incidence of 81 piglets with diarrhea was most prevalent as 39.5% in the age of 2 to 4 weeks, compare to 34.6% in the age of 5 to 8 weeks and 16.0% under 1 weeks and 9.9 % in the age of 9 to 11 weeks after birth. 2. The incidence of 81 piglets diarrhea showed bacterial diarrhea(75.3%), viral diarrhea (35.8%) and parasitological diarrhea(18.5%). When compared the isolation frequency of each etiological agent, enteropathogenic E. coli was most prevalent as 55.5% in bacterial diarrhea, rotavlrus enteritis as 18.5% in viral diarrhea and trichuriasis as 13.6% in parasitological diarrhea. 3. The complicated infection of piglets was most prevalent as 41.7% in rotavirus enteritis with enteropathogenic E coli in 24 complicated piglets diarrhea. 4. In antibiotic sensitivity test, isolates showed moderatly resistance to Tobramycin, Amikacin, Imipenem, Trimethoprim/Sulfamethoxazole, Gentamicin, Ampicillin but sensitivty to Ticarcillin/K. The 30 E. coli isolate showed multiple drug resistances in 3 different antibiotics.