• 제목/요약/키워드: PCoA

검색결과 153건 처리시간 0.032초

목질섭식곤충의 장내 세균 다양성 분석 및 섬유소 분해균 탐색 (Analysis of gut bacterial diversity and exploration of cellulose-degrading bacteria in xylophagous insects)

  • 최민영;안재형;송재경;김성현;배진우;원항연
    • 미생물학회지
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    • 제51권3호
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    • pp.209-220
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    • 2015
  • 목질 섭식 곤충에 관한 장내 세균 군집의 연구를 이용한 lignocellulose의 분해는 생명 공학적 응용에 있어 큰 잠재력을 갖는다. 본 연구에서 목질 섭식 곤충의 장내 세균 군집은 16S rRNA 유전자를 기반으로 한 파이로시퀀싱 방법을 이용하여 분석되었다. 분석된 모든 곤충에서 중장보다 후장에서 OTU수, 종 풍부도, 다양성 지수가 높았다. 세균 문 또는 강 수준의 다양성을 분석한 결과, 흰개미를 제외한 곤충의 장내 군집에는 Firmicutes, Bacteroidetes, ${\gamma}-Proteobacteria$가 우점하였다. PCoA (principal coordinates analysis)를 이용하여 세균의 군집 구조를 분석한 결과, 서식지보다는 곤충의 과별로 클러스터링 되는 경향이었다. CMC 분해 활성이 가장 높은 두 균주는 Bacillus toyonensis $BCT-7112^T$와 Lactococcus lactis subsp. hordniae $NCDO\;2181^T$과 유연관계가 높았다. 장 적출물의 섬유소 분해활성 실험 결과, 하늘소 후장에서 ${\beta}-1,4-glucosidase$, ${\beta}-1,4-endoglucanase$, ${\beta}-1,4-xylanase$의 효소활성이 가장 높았다. 본 연구에서는 목질 섭식 곤충의 장내에 다양하고 풍부한 세균이 서식하며, 섬유소를 분해하는 세균이 존재한다는 사실을 확인하였고, 이로부터 다양하고 유용한 섬유소 분해균을 분리할 수 있을 것으로 판단되었다.

대사에너지가 열 스트레스에 노출된 오리의 혈액 생체지표에 미치는 영향 (Influence of metabolizable energy on blood biomarkers in duck under heat stress)

  • 심준보;최두형;김창래;박병성
    • 한국응용과학기술학회지
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    • 제34권1호
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    • pp.132-141
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    • 2017
  • 본 연구의 목표는 열 스트레스 하에서 오리사료 내 대사에너지(ME) 수준이 혈액 매개변수에 미치는 영향을 조사하는 것이었다. 총 240마리의 육용 오리 채리밸리((Anas platyrhynchos)를 4처리구로 완전임의배치 한 후 42일 동안 사육하였다. 처리구는 ME 2900 kcal/kg, ME 3000 kcal/kg, ME 3100 kcal/kg 및 ME 3200 kcal/kg로 구분하였다. 혈액 지질 프로파일은 ME 3000과 비교할 때 ME 2900이 높았으나 ME 3100과 ME 3200은 낮았다 (p < 0.05). 혈액 aspartate aminotransferase (AST)와 alanine aminotransferase (ALT) 수준은 ME 3000과 비교할 때 ME 3100과 ME 3200에서 증가하였다 (p < 0.05). 혈액 적혈구와 혈소판 프로파일은 ME 3000과 비교할 때 ME 3100과 ME 3200은 높아졌으나 ME 2900은 감소하였다 (p < 0.05). 혈액 전해질 가운데, chloride ($Cl^-$) 농도는 ME 3000과 비교할 때 ME 2900에서 낮아졌다 (p < 0.05). 혈액 가스와 $PCO_2$는 ME 3000과 비교할 때 ME 2900에서 감소하였다 (p < 0.05). 혈액 면역물질(IgG) 수준은 ME 3000과 비교할 때 ME 2900에서 줄어들었다 (p < 0.05). 스트레스 호르몬, 코르티코스테론은 ME 3000과 비교할 때 ME 2900에서 높아졌으나 ME 3100과 ME 3200은 낮아졌다 (p < 0.05).

PD-DESYNC: Practical and Deterministic Desynchronization in Wireless Sensor Networks

  • Hyun, Sang-Hyun;Kim, Geon;Yang, Dongmin
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • 제13권8호
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    • pp.3880-3899
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    • 2019
  • Distributive desynchronization algorithms based on pulse-coupled oscillator (PCO) models have been proposed for achieving collision-free wireless transmissions. These algorithms do not depend on a global clock or infrastructure overheads. Moreover, they gradually converge to fair time-division multiple access (TDMA) scheduling by broadcasting a periodic pulse signal (called a 'firing') and adjusting the next firing time based on firings from other nodes. The time required to achieve constant spacing between phase neighbors is estimated in a closed form or via stochastic modeling. However, because these algorithms cannot guarantee the completion of desynchronization in a short and bounded timeframe, they are not practical. Motivated by the limitations of these methods, we propose a practical solution called PD-DESYNC that provides a short and deterministic convergence time using a flag firing to indicate the beginning of a cycle. We demonstrate that the proposed method guarantees the completion of desynchronization within three cycles, regardless of the number of nodes. Through extensive simulations and experiments, we confirm that PD-DESYNC not only outperforms other algorithms in terms of convergence time but also is a practical solution.

체외순환시 호기말 이산화탄소압의 변화 (Change of End-tidal PCS During Cardiopulmonary Bypass)

  • 오중환
    • Journal of Chest Surgery
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    • 제25권12호
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    • pp.1399-1403
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    • 1992
  • The evaluation of the effectivess of ongoing cardiopulmonary resucitation efforts is dependent on the commonly used methods, such as the presence of femoral or carotid artery pulsations, arterial blood gas determinations, peripheral arterial pressure and intracardiac pressure monitoring. But recent studies suggest that end-tidal carbon dioxide tension serves as a non-invasive measurement of pulmonary blood flow and therefore cardiac output under constant ventilation. A prospective clinical study was done to determine whether end-tidal carbon dioxide monitoring in open heart surgery under cardiopulmonary bypass could be used as a prognostic indicator of bypass weaning. We monitored end-tidal PCO2 values continuously during cardiopulmonary bypass in 30 patients. "Ohmeda 5210 CO-2 monitor" under infrared absorption method were incorperated into the ventilator circuit by means of a side point adaptor between endotracheal tube and ventilator tubing. 18 patients[Group I ] were res-ucitated from partial bypass followed by aorta cross clamp off and 12 patients[Group II ] from aorta cross clamp off followed by partial bypass. But there was no difference between two groups[p>0.05]. The value of end-tidal carbon dioxide tension during ventricular fibrillation or nearly arrest state was 6.6$\pm$2.9 mmHg, and at the time of spontaneous beating was 19.3$\pm$5.6 mmHg[Mean$\pm$Standard deviation], In conclusion end-tidal carbon dioxide tension monitoring provides clinically useful, continous, noninvasive and supplementary prognostic indicator during cardiopulmonary bypass weaning procedures.rocedures.

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LRCN을 이용한 리튬 이온 배터리의 건강 상태 추정 (State of Health Estimation for Lithium-Ion Batteries Using Long-term Recurrent Convolutional Network)

  • 홍선리;강모세;정학근;백종복;김종훈
    • 전력전자학회논문지
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    • 제26권3호
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    • pp.183-191
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    • 2021
  • A battery management system (BMS) provides some functions for ensuring safety and reliability that includes algorithms estimating battery states. Given the changes caused by various operating conditions, the state-of-health (SOH), which represents a figure of merit of the battery's ability to store and deliver energy, becomes challenging to estimate. Machine learning methods can be applied to perform accurate SOH estimation. In this study, we propose a Long-Term Recurrent Convolutional Network (LRCN) that combines the Convolutional Neural Network (CNN) and Long Short-term Memory (LSTM) to extract aging characteristics and learn temporal mechanisms. The dataset collected by the battery aging experiments of NASA PCoE is used to train models. The input dataset used part of the charging profile. The accuracy of the proposed model is compared with the CNN and LSTM models using the k-fold cross-validation technique. The proposed model achieves a low RMSE of 2.21%, which shows higher accuracy than others in SOH estimation.

Assessment of Genetic Diversity and Population Structure on Kenyan Sunflower (Helianthus annus L.) Breeding Lines by SSR Markers

  • Mwangi, Esther W.;Marzougui, Salem;Sung, Jung Suk;Bwalya, Ernest C.;Choi, Yu-Mi;Lee, Myung-Chul
    • 한국자원식물학회지
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    • 제32권3호
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    • pp.244-253
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    • 2019
  • In crop breeding program, information about genetic dissimilarity on breeding resources is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations and inbred breeding. This study aimed to evaluate the genetic variation in Kenyan sunflower breeding lines based on simple sequence repeat (SSR). A total of 83 alleles were detected at 32 SSR loci. The allele number per locus ranged from 2 to 7 with an average of 2.7 alleles per locus detected from the 24 sunflower accessions and the average value of polymorphic information contents (PIC) were 0.384. A cluster analysis based on the genetic similarity coefficients was conducted and the 24 sunflower breeding resources were classified into three groups. The principal coordinates (PCoA) revealed 34% and 13.38% respectively, and 47.38% of total variation. It was found that the genetic diversity within the Kenyan sunflower breeding resources was narrower than that in other sunflower germplasm resources, suggesting the importance and feasibility of introducing elite genotypes from different origins for selection of breeding lines with broader genetic base in Kenyan sunflower breeding program.

초위성체 마커를 이용한 한국 재래 흑염소의 유전적 다양성 및 유연관계 분석 (Analysis of Genetic Diversity and Relationships of Korean Native Black Goat using Microsatellite Markers)

  • 박병규;김이슬;성지연;공홍식
    • 한국동물생명공학회지
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    • 제34권3호
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    • pp.183-189
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    • 2019
  • The aim of this study was to assess the levels of genetic diversity and relationships of Korean native black goat (n = 58), compared with the exotic breed, Boer (n = 97). For the analysis of genetic characterization 11 microsatellite markers (MAF065, INRA063, CSRD247, OarFCB20, SRCRSP5, INRA006, ILSTS008, ILSTS011, INRA005, ILSTS087, SRCRSP8) were genotyped. The number of alleles was observed 3 (INRA005) to 10 (SRCRSP8) each markers. The mean expected and observed heterozygosity (Hexp and Hobs) and polymorphism information content (PIC) for the Korean native black goat breed varied from 0.551 to 0.860, 0.517 to 0.948 and 0.464 to 0.835, respectively. Principal Components Analysis (PCoA) and FCA results showed that Korean native black goat breed was confirmed to be clearly separated from bore breed. These results were scientific evidence that Korean native black goat represents a unique and valuable animal genetic resource.

행소음전탕액(杏蘇飮煎湯液)이 가토혈장(家兎血漿) COTISOL농도(濃度) 및 $PCO_2,\;CO_2$와 GUINEA PIG의 기관지(氣管支) 평활근(平滑筋)에 미치는 영향(影響) (Studies on the Effects of Haengsoyeum (杏蘇飮) Water Extract on the Plasma Cortisol Concentration in the Rabbit and the Contraction of Isolated Guinea Pig Tracheal Smooth Muscle)

  • 이승호
    • 대한한방내과학회지
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    • 제13권1호
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    • pp.143-155
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    • 1992
  • The following results were obtained from the observation on the change of plasma cortisol concentration in the experiment of intravenous administration of Haengsoyeum Water Extract in the rabbit. And the effects of Haengsoyeum extract on the contractile force of the isolated guinea pig trachea smooth muscle. 1. In intravenous administration the plasma cortisol concentration increased significantly about 1 hours after with a does of 0.2 ml/kg. 2. In intravenous administration the plasma cortisol concentration increased significantly about from 1 to 3 hours after with a does of 0.4 ml/kg. 3. The contractile response of the trachea smooth muscle of isolated guinea pig to histamine $10^{-4}\;M$ was significantly inhibited by Haengsoyeum extract. 4. The contractile response of the trachea smooth muscle of isolated guinea pig to acetylcholine $10^{-4}\;M$ was considerably inhibited by Haengsoyeum extract. 5. The contractile response of the trachea smooth muscle of isolated guinea pig to 5-hydroxytryptamine $10^{-4}\;M$ was inhibited by Haengsoyeum extract.

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Time-resolved PIV와 POD기법을 이용한 유량에 따른 단일노즐 버블링 유동 특성에 관한 연구 (Characteristics of Bubble-driven Flow with Varying Flow Rates by Using Time-resolved PIV and POD Technique)

  • 이승재;김종욱;김현동;김경천
    • 한국가시화정보학회지
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    • 제6권2호
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    • pp.14-19
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    • 2008
  • In this paper, the recirculation flow motion and mixing characteristics driven by air bubble flow in a rectangular water tank is studied. The Time-resolved PIV technique is adopted for the quantitative visualization and analysis. 532 nm Diode CW laser is used for illumination and orange fluorescent particle images are acquired by a PCO 10bit high-speed camera. To obtain clean particle images, 545 nm long pass optical filter and an image intensifier are employed and the flow rates of compressed air is changed from 2 l/min to 4 l/min at 0.5 MPa. The recirculation and mixing flow field is further investigated by the POD analysis technique. It is observed that the large scale counterclockwise rotation and main vortex is generated in the upper half depth from the free surface and one quarter width from the sidewall. When the flow rates are increased, the main vortex core is moved to the side and bottom wall direction.

Time-resolved PIV와 POD기법을 이용한 단일노즐 버블링 유동 특성에 관한 연구 (Characteristics of Bubble-driven Flow by Using Time-resolved PIV and POD Technique)

  • 이승재;김종욱;김현동;김경천
    • 한국가시화정보학회지
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    • 제6권1호
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    • pp.41-46
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    • 2008
  • In this paper, the recirculation flow motion and mixing characteristics driven by air bubble stream in a rectangular water tank is studied. The time-resolved PIV technique is adopted for the quantitative visualization and analysis. 488 nm Ar-ion CW laser is used for illumination and orange fluorescent ($\lambda_{ex}=540nm,\;\lambda_{em}=560nm$) particle images are acquired by a PCO 10bit high-speed CCD camera (1280$\times$1024). To obtain clean particle images, 545 nm long pass optical filter and an image intensifier are employed and the flow rates of compressed air is 3 l/min at 0.5 MPa. The recirculation and mixing flow field is further investigated by time-resolved POD analysis technique. It is observed that the large scale recirculation resulting from the interaction between rising bubble stream and side wall is the most dominant flow structure and there are small scale vortex structures moving along with large scale recirculation flow. It is also verified that the sum of 20 modes of velocity field has about 67.4% of total turbulent energy.