• Journal of Applied Biological Chemistry
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• v.62 no.4
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• pp.433-439
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• 2019

Artemisia species are widely used as food ingredients and raw material in traditional medicine. However, to date, the secondary metabolites of Artemisia gmelinii Weber ex Stechm. have not been sufficiently investigated. The secondary metabolites of A. gmelinii, which was collected from representative regions in Chungbuk, Gangwon, and Gyeongbuk, were analyzed using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTof MS) combined with an unsupervised principal component analysis (PCA) multivariate analysis. In the loading scatter plot of PCA, significant changes in metabolites were observed between the regions, ten metabolites (3: 5-O-caffeoylquinic acid, 4: 4-O-caffeoylquinic acid, 8: trans-melilotoside, 12: quercetin 3-O-hexoside, 15: 3,4-O-dicaffeoylquinic acid, 17: 3,5-O-dicaffeoylquinic acid, 18: 4,5-O-dicaffeoylquinic acid, 19: syringaldehyde, 20: caffeoylquinic acid derivative, and 23: icariside II) were evaluated as key markers among twenty-five identified metabolites. Interestingly, the contents of the identified marker significantly differed between the three groups. This is the first study to report the presence of marker metabolites and their correlating geographical cultivation in A. gmelinii.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.3
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• pp.371-374
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• 2011

The objectives of the present study evaluated aquatic animals on the water in a rice field. Field investigation was carried out in conventional tillage without rice straw or green manure crop treatment (CTFS, check plot), no-tillage without cover crops (NTNT), no-tillage amended with rape (NTRA), no-tillage amended with rye (NTRY), no-tillage amended with hairyvetch (NTHV), and no-tillage amended with Chinese milk vetch (NTCM). Total dense population of aquatic animals in HTHV was significantly higher than the other plots (p<0.05) on May 30. Dense populations of Daphniidae and Culicidae on June 20 were lowest in CTFS compared to no-tillage plots (p<0.05). Furthermore, in principal component analysis (PCA), PC1 explained 44.9% of variance, whereas PC2 explained 26%, for a cumulative total of 70.9% and the PC1 of the PCA separated the samples from NT treatments and CFS (p<0.05).

• The Plant Pathology Journal
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• v.34 no.6
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• pp.544-554
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• 2018

Tomato (Solanum lycopersicum) production in Ghana is limited by the root-knot nematode (Meloidogyne incognita, and yield losses over 70% have been experienced in farmer fields. Major management strategies of the root-knot nematode (RKN), such as rotation and nematicide application, and crop rotation are either little efficient and harmful to environments, with high control cost, respectively. Therefore, this study aims to examine morphometric variations of RKN populations in Ghana, using principal component analysis (PCA), of which the information can be utilized for the development of tomato cultivars resistant to RKN. Ninety (90) second-stage juveniles (J2) and 16 adult males of M. incognita were morphometrically characterized. Six and five morphometric variables were measured for adult males and second-stage juveniles (J2) respectively. Morphological measurements showed differences among the adult males and second-stage juveniles (J2). A plot of PC1 and PC2 for M. incognita male populations showed clustering into three main groups. Populations from Asuosu and Afrancho (Group I) were more closely related compared to populations from Tuobodom and Vea (Group II). There was however a single nematode from Afrancho (AF4) that fell into Group III. Biplots for male populations indicate, body length, DEGO, greatest body width, and gubernaculum length serving as variables distinguishing Group 1 and Group 2 populations. These same groupings from the PCA were reflected in the dendogram generated using Agglomerative Hierarchical Clustering (AHC). This study provides the first report on morphometric characterisation of M. incognita male and juvenile populations in Ghana showing significant morphological variation.

• Journal of Practical Agriculture & Fisheries Research
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• v.22 no.2
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• pp.59-67
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• 2020

Growth tests on the Wando and Baengnyeongdo cultivars of Saccharina japonica were performed at the Myeongcheon and Gyedo aquafarms, Goheung in Jeollanamdo, from February to July in 2003. Five environmental conditions and 2 traits were measured monthly. The data were used to analyze the growth patterns, relationships between traits and principal component. Box plots were used to display the growth patterns. Scatter plots and regression and correlation coefficients were used to determine the strength of relationships between the traits. A principal component analysis revealed that the first principal component explained more than 91.4% and 90.5% of the total sample variance in the Myeongcheon and Gyedo aquafarms. From the viewpoint of the economic traits (blade length, blade weight), the growth of populations from the Gyedo aquafarm was stronger than that of those from the Myeongcheon aquafarm, and the growth of the Baengnyeongdo cultivar was superior to that of the Wando one.

• Journal of the Korean Society of International Agriculture
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• v.31 no.4
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• pp.378-383
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• 2019

To determine whether FT-IR spectral analysis based on multivariate analysis for whole cell extracts can be used to discriminate papaya at metabolic level. FT-IR spectral data from leaves were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). FT-IR spectra confirmed typical spectral differences between the frequency regions of 1,700-1,500, 1,500-1,300 and 1,100-950 cm^-1, respectively. These spectral regions were reflecting the quantitative and qualitative variations of amide I, II from amino acids and proteins (1,700-1,500 cm^-1), phosphodiester groups from nucleic acid and phospholipid (1,500-1,300 cm^-1) and carbohydrate compounds (1,100-950 cm^-1). The result of PCA analysis showed that papaya leaves could be separated into clusters depending on different growth temperature. In this case, showed discrimination confirmed according to metabolite content of growth condition from papaya. And PLS-DA analysis also showed more clear discrimination pattern than PCA result. Furthermore, these metabolic discrimination systems could be applied for rapid selection and classification of useful papaya cultivars.

• Weed & Turfgrass Science
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• v.4 no.3
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• pp.176-187
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• 2015

The weed flora in the Codonopsis lanceolata upland fields were surveyed in Hoengseong and Jeju in May, August, and October of 2014. The objectives of this study were to use the survey data for establishing weed control methods and to bring awareness of possible problematic weeds occurred in C. lanceolata upland fields in Korea. Altogether 207 weed species of 47 families were identified, of which 87 were annual, 47 species were biennial and 73 were perennial. Based on the morphological characteristic of the leaves, 182 species were broad leaves, 18 species were grasses, and 7 species were sedges. The dominance was highest in the Artemisia princeps followed by Digitaria ciliaris, Commelina communis, Persicaria longiseta and Echinochloa crus-galli etc. Fifty-nine species were exotic weeds with 29% of a total presence, of which Erigeron annuus was highest, followed by Crassocephalum crepidioides, Bidens frondosa, Hypochaeris radicata etc. In the PCA plot, weeds presented in the C. lanceolata upland fields of Hoengseong were divided into three groups, E. annuus, Sigesbeckia pubescence and C. communis communities and those of Jeju were divided into four groups, C. crepidioides-Cyperus iria, Spergula arvensis, Poa annua and Youngia japonica communities.

• Korean Journal of Agricultural Science
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• v.36 no.2
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• pp.219-224
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• 2009

Two functional modified-butterfats (MF668 and MF866) were synthesized with two blends (6:6:8 and 8:6:6, w/w%) of anhydrous butterfat (ABF), palm stearin (PS) and flaxseed oil (FSO, omega-3) via lipase-catalyzed interesterification reaction. Their flavor characteristic was investigated using electronic nose and SPME-GC/MS analysis. Each flavor pattern of ABF, FSO, MF668 and MF866 was significantly discriminated with first principal component score of 95.16% in PCA plot. In functional modified-butterfats analyzed with SPME-GC/MS, various volatile compounds such as aldehydes, ketones, acids, and alkanes were detected.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.1971-1981
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• 2018

In this study, we investigated the altered enzymatic activities and metabolite profiles of koji fermented using varying permutations of Aspergillus oryzae and/or Bacillus amyloliquefaciens. Notably, the protease and ${\beta}$-glucosidase activities were manifold increased in co-inoculated (CO) koji samples (co-inoculation of A. oryzae and B. amyloliquefaciens). Furthermore, gas chromatography-mass spectrometry (GC-MS)-based metabolite profiling indicates that levels of amino acids, organic acids, sugars, sugar alcohols, fatty acids, nucleosides, and vitamins were distinctly higher in CO, SA (sequential inoculation of A. oryzae, followed by B. amyloliquefaciens), and SB (sequential inoculation of B. amyloliquefaciens, followed by A. oryzae). The multivariate principal component analysis (PCA) plot based on GC-MS datasets indicated a clustered pattern for MA and MB (koji samples inoculated either with A. oryzae or B. amyloliquefaciens) across PC2 (20.0%). In contrast, the CO, SA, and SB metabolite profiles displayed segregated patterns across PLS1 (22.2%) and PLS2 (21.1%) in the partial least-square discriminant analysis (PLS-DA) model. Intriguingly, the observed disparity in the levels of primary metabolites was engendered largely by higher relative levels of sugars and sugar alcohols in MA, SA, and CO koji samples, which was commensurate with the relative amylase activities in respective samples. Collectively, the present study emphasizes the utility of integrated biochemical and metabolomic approaches for achieving the optimal permutation of fermentative inocula for industrial koji preparation.

• Korean journal of food and cookery science
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• v.17 no.4
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• pp.353-358
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• 2001

The leaves of Duchung(Eucommia ulmoides), an oriental medicinal plant, have a peculiar aroma of Chinese medicine and astringent taste, which make the consumer be reluctant to Duchung leaf tea. Therefore, the purpose of this study was to develop a mild flavored Duchung leaf tea by mixing with other plants. The flavor patterns of developed tea were analyzed using an electronic nose. Polygonatum odoratum and Elsholtzia splendens were used for improving the flavor of Duchung leaf tea. The addition of 20, 30 and 40% of Polygonatum odoratum improved the overall acceptance in hedonic sensory evaluation. The flavor pattern of the tea was described by principal component analysis(PCA) and the resistance ratio(R/cub gas/R$\_$air/) of sensors. The PCA plot was also used to explain the mild flavor of the tea, which was extended from the right side(positive value of the first principal component) to the left side(negative value). Analysis by using an electronic nose with metal oxide sensors could be applied to detect whether mixed Duchung leaf tea was acceptable or not.

• Korean Journal of Food Science and Technology
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• v.26 no.5
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• pp.649-654
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• 1994

In order to provide a quality index of Korean folk sojues, physicochemical properties of Korean folk sojues (Andong soju, Moonbaesul, Leekangju, Jindo Hongju, Chebiwon soju, Yethyang (rice) and Yethyang (barley)), Paekrosul, Chinese kaoliangchiews (Moutaichiew, Ergoutoutiu, Chuyehchingchiew, Zhikukaoliangchiew and Paigal), Japanese Senbatanuki shochu and two whiskies were determined. The pH of sojues ($3.43{\sim}5.85$) were mainly influenced by total acidity which was described as acetic acid. The conductivities of Paekrosul and Leekangju showed relatively high value of $246\;and\;122.7{\mu}S/cm$, respectively. Korean folk sojues and Paekrosul showed maximum absorption at 274 or $278{\sim}280nm$ in the spectrometric study. We performed principal components analysis (PCA) of physicochemical properties and spectrometric data to extract representative characteristics and to compare the similarity or the dissimilarity. The PCA plot showed the distinguished cluster of Korean folk sojues comparing with Chinese kaoliangchiew or Paekrosul etc.