• 한국가금학회지
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• 제34권2호
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• pp.129-136
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• 2007

본 연구는 한국산 참나무 겨우살이를 육계에 급여하였을 때 생산성 및 혈액 특성에 미치는 영향을 구명하기 위해 실시하였다. 1일령 Ross 수평아리를 4처리 4반복, 반복당 30수씩 총 480수를 공시하였고, 실험 설계는 무항생제 사료, 항생제 첨가 사료, 겨우살이 0.5% 및 1.0% 처리구로 나누어 총 49일간 사육하였다. 실험 전 기간 처리구간에 사료 섭취량과 일당 증체량에 있어서는 유의차가 없었다. 실험 종료시 공장의 길이에 있어서 겨우살이 1.0% 처리구가 다른 처리구에 비해 증가하는 경향이나 유의하게 증가하였다(P<0.05). 실험 35일째, 맹장내 살모넬라 균수는 겨우살이를 급여함으로써 유의하게 감소하였다(P<0.05). 겨우살이 처리구에서는 대조구(+)에 비해 림프구와 단핵구의 수가 증가하는 경향 또는 유의하게 증가하였다(P<0.05). 따라서, 겨우살이의 사료 내 첨가급여는 육계에서 생산성 및 혈액 성상에 아무런 부정적인 영향을 미치지 않아 항생제 대체 물질로서의 이용 가능성이 있을 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권2호
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• pp.227-232
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• 2013

Phytate is not only an unavailable source of phosphorus (P) for broilers but it also acts as an anti-nutrient, reducing protein and mineral absorption, increasing endogenous losses and reducing broiler performance. The objective of this study was to investigate the anti-nutritional effects of phytate by including high levels of phytase in diets not severely limited in available P. A total of 768 male Arbor Acres broilers were distributed in six treatments of eight replicate pens of 16 birds each consisting of a positive control diet (PC), positive control with 500 FTU/kg phytase, negative control (NC) diet with lower available P and calcium (Ca) levels and the same NC diet with 500, 1,000 or 1,500 FTU/kg phytase. Body weight gain (BWG), feed intake (FI), feed conversion ratio (FCR) and mortality were determined at 21 and 35 d of age while foot ash was determined in four birds per pen at 21 d of age. FI, FCR and foot ash where not affected by the lower mineral diets at 21 d of age nor by the enzyme inclusion but broilers fed lower Ca and available P diets had lower BWG. At 35 d of age no difference was observed between broilers fed the positive or NC diets but broilers fed 500, 1,000 and 1,500 FTU/kg on top of the NC diet had better FCR than broilers fed the positive control diet. When compared to birds fed a diet adequate in P, birds fed the same diet included with 500, 1,000 and 1,500 FTU/kg of phytase in marginally deficient available P and Ca diets had an improvement of performance. These results support the concept that hydrolysing phytate and reducing the anti-nutritional effects of phytate improves bird performance on marginally deficient diets that were not covering the P requirement of birds.

• Nutrition Research and Practice
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• 제10권6호
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• pp.569-574
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• 2016

BACKGROUND/OBJECTIVES: We investigated the anti-osteoarthritic effects of deer bone extract on the gene expressions of matrix metalloproteinases (MMPs) and collagen type II (COL2) in interleukin-$1{\beta}$-induced osteoarthritis (OA) chondrocytes. MATERIALS/METHODS: Primary rabbit chondrocytes were treated as follows: CON (PBS treatment), NC (IL-$1{\beta}$ treatment), PC (IL-$1{\beta}+100{\mu}g/mL$ glucosamine sulphate/chondroitin sulphate mixture), and DB (IL-$1{\beta}+100{\mu}g/mL$ deer bone extract). RESULTS: The results of the cell viability assay indicated that deer bone extract at doses ranging from 100 to $500{\mu}g/mL$ inhibits cell death in chondrocytes induced by IL-$1{\beta}$. Deer bone extract was able to significantly recover the mRNA expression of COL2 that was down-regulated by IL-$1{\beta}$ (NC: 0.79 vs. DB: 0.87, P < 0.05) and significantly decrease the mRNA expression of MMP-3 (NC: 2.24 vs. DB: 1.75) and -13 (NC: 1.28 vs. DB: 0.89) in OA chondrocytes (P < 0.05).CONCLUSIONS: We concluded that deer bone extract induces accumulation of COL2 through the down-regulation of MMPs in IL-$1{\beta}$-induced OA chondrocytes. Our results suggest that deer bone extract, which contains various components related to OA, including chondroitin sulphate, may possess anti-osteoarthritic properties and be of value in inhibiting the pathogenesis of OA.

• 한국정밀공학회지
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• 제10권2호
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• pp.114-125
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• 1993

In the present study the newly developed CAD/CAM system is applied to the process of the molding design, machining for mini-sized and precise processive die, and the production of press-stamped parts. When the design of a die was completed by means of CAD, wire cut NC data were generated with the aid of a design drawing in the CAD system and then inputed into the wire cut machine, and with the aid of a hole chart which had been made for this purpose, all the data were classified into the categories of CNC milling, jig boring, jig grinding, and machine center, and then developing a program of generating NC data, errors in process were reduced and programming time was shortened. The program was developed by using Autolisp language which was built-in the CAD, and realizing the intergation of designing a die, generating and processing NC data directly by a designer, designing time and machinery processing time were shorted. And the traditionally required working time for design. NC program required 6 days of work becomes 4 days of work by using the developed CAD/CAM system so that the efficiency shows 150% of the reduction working time. The prpgram of the design of the automation a progressive die mold was developed in the PC-Class Autocad system, therefore development expense could be reduced, and the integration of the CAD/CAM of the progressive die mold with the standard DB being built could be realized.

• 대한한방소아과학회지
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• 제22권2호
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• pp.81-114
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• 2008

Objectives : The purpose of this study is to investigate the effect of Jeseupwiryeongtang-gagam(JWTG) on atopic dermatitis by in vivo experiment using NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the atopic dermatitis of human. Methods : To investigate the effect of JWTG on AD, we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells(PBMCs), splenocytes, draining lymph node(DLN) and performed skin histology in ears and dorsal skin of atopic dermatitis-like skin NC/Nga mouse in vivo. Results and Conclusions : In vivo, clinical skin severity score were significantly lower in JWTG group than control group. IgE, IL-6, $TNF-{\alpha}$, IgM, IgG2a and IgG2b levels in serum were decreased remarkably in JWTG group than control group and $IFN-{\gamma}$ production, secreted in Th1 cell were increased by JWTG. After experiment ended, we analyzed immunological cells ($CD3^+$, $CD19^+$, $CD4^+$, $CD8^+$, $CD3^+$$CD69^+$, $CD4^+$$CD25^+$ and $CD49b^+$) by flow cytometry. It resulted that total absolute number of $CD3^+$, $CD19^+$, $CD4^+$ and $CD8^+$ cells were recovered as normal and $CD3^+$$CD69^+$ were decreased significantly compared with control group in isolated DLN and PBMCs from NC/Nga mouse and total absolute number of $Gr-1^+$, $CD11b^+$ and $CD3^+$ in dorsal skin of NC/Nga mouse were decreased by JWTG. We analyzed ear, DLN, and neck-back skin after biopsy and dyeing by hematoxyline/eosin(H&E) and toluidine staining (mast cells marker) and obtained results that JWTG were effective to histological symptoms (dermal and epidermal thickening, hyperkeratosis and inflammatory cell infiltration). Ear thickness was decreased significantly than the control group and the size of inflammatory lymphocytes cells(ILC) and plasma cells(PC) in DLN were also decreased.

• 대한한방소아과학회지
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• 제22권1호
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• pp.69-93
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• 2008

Objectives The purpose of this study is to investigate the effect of KKHS on atopic dermatitis in an in-vivo experiment using an NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to this condition in humans. Methods To investigate the effect of KKHS on atopic dermatitis (AD), we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells(PBMCs), splenocytes, draining lymph node(DLN) and performed skin histology in ears and dorsal skin of atopic dermatitis of NC/Nga mouse in vivo. Results In vivo, clinical skin severity score was significantly lower in the KKHS group than in the control group. IgE, IL-6, TNF-${\alpha}$, IgM, IgG2a and IgG2b levels in serum decreased remarkably in the KKHS group than in the control group, and the level of IFN-${\gamma}$ production which is secreted from Th1 cell was increased by KKHS. After this experiment we analyzed immunological cells ($CD3^+$, $CD19^+$, $CD4^+$, $CD8^+$, $CD3^+CD69^+$, $CD4^+CD25^+$ and $CD49b^+$) by flow cytometry. It results that the total absolute number of $CD3^+$, $CD19^+$, $CD4^+$ and $CD8^+$ cells were recovered as much as normal state, and the level of $CD3^+CD69^+$ in isolated DLN and PBMCs were significantly decreased, and total absolute number of $Gr-1^+$, $CD11b^+$ and $CD3^+$ in dorsal skin of NC/Nga mouse were decreased by KKHS. We analyzed ear, DLN, and neck-back skin after biopsy and dyeing by hematoxyline/eosin(H&E), toluidine staining (mast cells marker). KKHS were very effective to the histological symptoms which are in dermal and epidermal thickening, hyperkeratosis and inflammatory cell infiltration. Ear thickness was significantly decreased compared with the control group and the size of inflammatory lymphocytes cells (ILC) and plasma cells (PC) in DLN were also decreased. Conclusions KKHS on atopic dermatitis in an in-vivo experiment using an NC/Nga atopic dermatitis mouse was very effectiveness to the atopy dermatitis treatment.

• 한국식품영양과학회지
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• 제42권8호
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• pp.1190-1196
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• 2013

연구에서는 백삼이나 홍삼의 기억력 개선 효과 연구를 기초로 흑삼의 기억력 개선효과 여부를 판단하고자 scopolamine으로 유도된 시험동물에게 7주간 시료 물질(백삼, WG; 홍삼, RG; 흑삼, BG) 추출액을 투여한 후, 행동학적인 평가 및 뇌 조직 내 malondialdehyde 농도, ChAT 활성 변화를 비교 분석하여 기억력 및 학습능력 손상에 대한 개선효과를 알아보고자 하였다. 수동회피시험에서 BG군과 RG군의 latency time이 scopolamine 투여한 PC군(positive control)에 비해서 유의적으로 길어지는 결과를 나타냈다. 수중미로시험에서도 BG군과 RG군의 scopolamine에 의한 기억 손상이 유의적으로 개선되어 NC군의 escape latency 수준 정도로 낮아짐을 확인할 수 있었다. 또한 probe test에서도 BG군과 RG군에서 장기 기억력 손상이 유의적으로 개선됨이 확인되었다. BG군과 RG군의 뇌조직 ChAT 효소 활성은 PC군에 비해 각각 42%, 71% 수준의 유의성 있는 활성증가를 보였다. 지질 과산화도 malondialdehyde 측정 결과에서 PC군 대비 RG군과 BG군에서 각각 37%, 33% 수준의 유의성 있는 감소를 보였다. 이상의 결과를 요약하면 시험물질 가운데 흑삼의 반복 경구투여는 scopolamine으로 유도된 흰쥐에서 기억력 감퇴를 개선하는 데 가장 효과적인 것으로 사료된다.

• 산업공학
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• 제10권1호
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• pp.135-143
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• 1997

The importance of training for NC, CNC and Machining Center has been greatly increased. This paper presents implementation of a DNC(Direct Numerical Control) operating software for educational system. This system is able to connect 8-32 CNCs to Control PC with RS232 multi-port serial card. Therefore, it allows much efficiency in training even after costs are considered. The KISCO DNC S/W for above system includes various communication functions, communication parameters setting, program editor and user-friendly environment. This software was developed with C and Windows programming. It was proved in function and stability by iterative field tests.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1996년도 한국자동제어학술회의논문집(국내학술편); 포항공과대학교, 포항; 24-26 Oct. 1996
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• pp.1328-1331
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• 1996

The importance of training for NC, CNC and Machining Center has been greatly increased. This paper presents implementation of a DNC system and operating software for educational purpose. This system is able to connect 8-32 CNCs to Control PC with RS232 multi-port serial card. Therefore, it allows much efficiency in training even after costs are considered. The KISCO DNC S/W for above system includes various communication function, communication parameter setting, program editor, tool management and user-friendly environment.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1992년도 추계학술대회 논문집
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• pp.228-232
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• 1992

The integrated management system IMMC system) is developed for machining, measuring and checking by using IBM-PC under Borland C $\^$++/ environment. MMC system consists of following systems : DNC system, MascMC system (for measuring and checking) and MascCAM system : DNC system, MascNC system (for measuring and checking) and MascCAM system (for CAM). And some other modules support MMC system for practical usages. A vertical machining center equipped with FANUC 0MC is used for experiments. Feasibility of the system is confirmed by a large amount of experiments.