• Clinics in Shoulder and Elbow
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• 제9권2호
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• pp.149-154
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• 2006

• 오토저널
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• 제22권2호
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• pp.82-85
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• 2000

• 동국한의학연구소논문집
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• 제2권2호
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• pp.99-124
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• 1993

• E2M - 전기 전자와 첨단 소재
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• 제12권2호
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• pp.24-33
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• 1999

• 한국CDE학회지
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• 제17권2호
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• pp.2-9
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• 2011

• 한국세라믹학회:학술대회논문집
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• 한국세라믹학회 2000년도 추계총회,초청강연,특별강연,연구발표회
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• pp.80.2-80
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• 2000

• BMB Reports
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• 제28권6호
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• pp.477-483
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• 1995

The recombinant human interferon alpha 2a ($rhIFN-{\alpha}2a$), expressed in Saccharomyces cerevtsiae, was purified from insoluble aggregates. The inclusion body of $rhIFN-{\alpha}$ was solubilized by guanidine salt in the presence of disulfide reducing agent. The refolding of denatured $rhIFN-{\alpha}2a$ was achieved by simple dilution. The authentic interferon alpha, which has two correctly matched disulfide bonds, was seperated from incompletely oxidized $IFN-{\alpha}$ and dimeric $IFN-{\alpha}$ by use of a CM-Sepharose column, followed by size exclusion columns at two different pH conditions. The purified protein has been subjected to detailed physicochemical characterization including sequence determination. Unlike other $rhIFN-{\alpha}2a$ from E. coli reported, the $rhIFN-{\alpha}2a$ from S. cerevisiae has no methionine residue at its N-terminus originating from the start codon, ATG. The pI of the protein was determined to be 6.05 with a single band in the pI gel, which demonstrated that the purified $rhIFN-{\alpha}$ was homogeneous. The structural study using circular dichroism showed that the protein retains its three dimensional structure in the wide range of pH conditions between pH 3 and 9, and only minor strucural deformation was observed at pH 1.0.

• 한국환경보건학회지
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• 제41권3호
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• pp.171-181
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• 2015

Objectives: This study aimed to survey the characteristics of air quality and meteorological conditions in a greenway park. Methods: We measured meteorological and health related factors, including noise, particulate matter ($PM_{10}$) and selected gaseous air pollutants at three locations in a greenway park and on a general roadside as comparison. The measurements were repeated four times from April to October 2014. Results: The average air temperature in the greenway park was $20.7^{\circ}C$ which was $1-2^{\circ}C$ lower than on the general roadside. The average $PM_{10}$ concentration in the greenway park was $85.0{\mu}g/m^3$, a level 2-3 times lower than that at the roadside. The noise level at the greenway site was 4.4 dB(A)- 23.0 dB(A) lower than at the roadside. The average CO, $CO_2$, $SO_2$ and NOx concentrations in the greenway park were lower than at the roadside. The average phytoncide and anions concentrations in the greenway park were higher than at the roadside. Conclusions: The urban forest of the greenway park may have some impact on air quality and meteorological conditions.

• 한국환경복원기술학회지
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• 제17권1호
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• pp.91-109
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• 2014

This study was conducted to obtain the basic research about Siberian roe deer (Capreolus pygargus), and to establish a conservation area into Seoraksan(Mt.) National Park, South Korea. We recorded Siberian roe deer field signs (bedding sites, feeding areas, feces and tracks etc), and habitat variables such as nearest distance to the watercourse, trails, slope, aspect, forest type and land cover etc. from Jan. 2006 to Dec. 2007 in Seoraksan(Mt.) National Park. According to the research of habitat characteristics of Siberian roe deer inhabited in Seoraksan(Mt.) National Park preferred mixed forest consisted of aged forest and middle-aged forest on the gentle slope which was close to hydrosphere in the middle elevation area of altitude of 400~600m above sea level in Seoraksan(Mt.) National Park. The results indicated that Siberian roe deer appeared to prefer south slope or north slope during the snow season and west or east slope during the non-snow season. The area of most suitable habitat for Siberian roe deer in Seoraksan(Mt.) National Park was $128.5km^2$ (32% of the total area) followed by $244.3km^2$ buffer area (62.2% of the total area), and $22.9km^2$ unsuitable habitat area (5.8% of the total area), which means environmentally unsuitable habitat for Siberian roe deer was rare in Seoraksan(Mt.) National Park. Thus, the area classified as major area in Seoraksan(Mt.) National Park appeared to be most important for the protection of Siberian roe deer.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2007년도 추계학술대회 논문집
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• pp.73-74
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• 2007