• Journal of the Semiconductor & Display Technology
• /
• v.5 no.3 s.16
• /
• pp.37-41
• /
• 2006

In this paper, a composition modulation of Dot-matrix display grayscale is presented. There's two method of expression of Dot-matrix Display grayscale. One is PWM by controlling pulse width. The other is PAM by controlling pulse amplitude. Composition modulation of Dot-matrix use PWM and PAM together. Composition modulation shows the number of grayscale as number of grayscale of PWM$\times$number of grayscale of PAM and have the effect of reduced size than existing single modulation.

• Bulletin of the Korean Chemical Society
• /
• v.17 no.11
• /
• pp.1036-1039
• /
• 1996

Synthetic lipoidal peptides based on viral protein sequences have been prepared. These peptides contain an N-palmitoyl group at the N-terminal residue, which is a modified cysteine, containing a S-[2,3-bis(acyloxy)-(2-R,S)-propyl] moiety. When this residue (Pam3Cys) is at the N-terminus of a synthetic peptide, it acts as potent immunoadjuvant to enhance both IgM and IgG antibody responses to the attached peptide. Conventional analytical procedures (e.g., Edman degradation and amino acid analysis) are either not applicable due to the N-terminal modification, or do not provide confirmation of the intact structure. Chromatographic analysis is also hindered by the tendency of these lipoidal Pam3Cys peptides to form large aggregates, and in some cases to be permanently adsorbed on reversed phase columns. We have applied several mass spectrometric techniques, including fast atom bombardment (FAB), electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI) to characterize the intact structures of a number of different Pam3Cys synthetic peptides. The MALDI-MS has been found to be the most sensitive for the analysis of the structure of Pam3Cys peptides.

• Interdisciplinary Bio Central
• /
• v.6 no.4
• /
• pp.3.1-3.10
• /
• 2014

Amino acid substitution matrices are essential tools for protein sequence analysis, homology sequence search in protein databases and multiple sequence alignment. The PAM matrix was the first widely used amino acid substitution matrix. The BLOSUM series then succeeded the PAM matrix. Most substitution matrixes were developed by using the statistical frequency of substitution between each amino acid at blocks representing groups of protein families or related proteins. However, substitution of amino acids is based on the similarity of physiochemical properties of each amino acid. In this study, a new approach was used to obtain major physiochemical properties in multiple sequence alignment. Frequency of amino acid substitution in multiple sequence alignment database and selected attributes of amino acids in physiochemical properties database were merged. This merged data showed the major physiochemical properties through principle components analysis. Using factor analysis, these four principle components were interpreted as flexibility of electronic movement, polarity, negative charge and structural flexibility. Applying these four components, BAPS was constructed and validated for accuracy. When comparing receiver operated characteristic ($ROC_{50}$) values, BAPS scored slightly lower than BLOSUM and PAM. However, when evaluating for accuracy by comparing results from multiple sequence alignment with the structural alignment results of two test data sets with known three-dimensional structure in the homologous structure alignment database, the result of the test for BAPS was comparatively equivalent or better than results for prior matrices including PAM, Gonnet, Identity and Genetic code matrix.

• Bioinformatics and Biosystems
• /
• v.2 no.1
• /
• pp.7-11
• /
• 2007

Methods for making scoring matrix based on phylogenetic tree for all possible exchanges of one amino acid with another. PFMT(Phylogenetic focused Mutation Tendency) matrix is different BLOSUM62 and PAM160 which are the most used scoring matrixes. This matrix calculates possibility of substitution from common ancestor to high spices. PFMT matrix scores substitution frequency in COGs databases which contain 152 KOGs's dataset. PFMT matrix usefully is able to compare between query sequence and sequences of more higher species and show detailed substitution relation of 20 amino acids.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.4
• /
• pp.1077-1081
• /
• 2014

Polyacrylamide (PAM) -HgS nanocomposites were successfully prepared in polyacrylamide (PAM) matrix. From TEM and XRD characterizations, the synthesized HgS nanocrystals were chain-like spherical in shape with a diameter of about 40-60 nm and high crystalline quality. The quantum-confined effect of HgS nanocrystals was confirmed by UV-vis diffuse reflection spectra. The optical properties of products were investigated by using photoluminescence (PL) spectra, which showed that HgS nanocrystals exhibited good optical properties with maximum emission peak at about 640 and 650 nm at different reaction temperatures. The interaction of HgS nanocrystals with PAM was studied through FT-IR spectroscopy and TG analysis, which suggested that $Hg^{2+}$ could interact with functional groups of PAM. The experimental results indicated that PAM not only induced nucleation, but also inhibited further growth of HgS crystals and play an important role in the formation of PAM/HgS nanocomposites. In addition, the possible mechanism of HgS nanoparticles growth in PAM solution was also discussed.

• The Journal of the Korea Contents Association
• /
• v.12 no.3
• /
• pp.34-43
• /
• 2012

Determining the similarity between two strings can be applied various area such as information retrieval, spell checker and spam filtering. Similarity calculation between Korean strings based on dynamic programming methods firstly requires a definition of the similarity between phonemes. However, existing methods have a limitation that they use manually set similarity scores. In this paper, we propose a method to automatically calculate inter-phoneme similarity from a given set of variant words using a PAM-like probabilistic model. Our proposed method first finds the pairs of similar words from a given word set, and derives derivation rules from text alignment results among the similar word pairs. Then, similarity scores are calculated from the frequencies of variations between different phonemes. As an experimental result, we show an improvement of 10.1%~14.1% and 8.1%~11.8% in terms of sensitivity compared with the simple match-mismatch scoring scheme and the manually set inter-phoneme similarity scheme, respectively, with a specificity of 77.2%~80.4%.

• Polymer(Korea)
• /
• v.30 no.6
• /
• pp.545-549
• /
• 2006

Blends of poly(acrylic acid- co-maleic acid) (PAM) with poly (vinyl alcohol) (PVA) were pre -pared in distilled water PVA/PAM/saponite (PVA/PAM/SPT) nanocomposite films were prepared with various clay contents by using the solution intercalation method. The variations of the dispersion, morphology, and thermo-mechanical properties of the nanocomposites with clay content in the range 0 to 9 wt% were examined. Up to 3 wt% clay loading, the clay particles were homogeneously dispersed in the PVA/PAM blends. However, some agglomerated structures form in the polymer matrix above a clay content of 7 wt%. The thermal stability of the hybrids was increased linearly with increasing the clay loading up to 9 wt%. The maximum strength and modulus were obtained at a clay content of 7 wt%. Thus, the addition of small amounts of clay to the PVA/PAM blends produced PVA/PAM nano-composites with improved the thermo-mechanical properties.

• International Journal of Fuzzy Logic and Intelligent Systems
• /
• v.3 no.2
• /
• pp.187-193
• /
• 2003

In this paper, a systematic design method of the intelligent PAM fuzzy controller for nonlinear systems using the efficient tools-Linear Matrix Inequality and the intelligent digital redesign is proposed. In order to digitally control the nonlinear systems, the TS fuzzy model is used for fuzzy modeling of the given nonlinear system. The convex representation technique also can be utilized for obtaining TS fuzzy models. First, the analog fuzzy-model-based controller is designed such that the closed-loop system is globally asymptotically stable in the sense of Lyapunov stability criterion. The simulation results strongly convince us that the proposed method has great potential in the application to the industry.

• ETRI Journal
• /
• v.43 no.5
• /
• pp.923-931
• /
• 2021

We studied the influence of GaAs top-layer thickness on the small-signal modulation response and 56 Gb/s four-level pulse-amplitude modulation eye quality of 850 nm vertical-cavity surface-emitting lasers (VCSELs). We considered the proportionality of the gain-saturation coefficient to the photon lifetime. The simulation results that employed the transfer-matrix method and laser rate equations led to the conclusion that the proportionality should be considered for proper explanation of the experimental results. From the obtained optical eyes, we could determine an optimum thickness of the GaAs top layer that rendered the best eye quality of VCSEL. We also compared two results: one result with a fixed gain-saturation coefficient and the other that considered the proportionality. The former result with the constant gain-saturation coefficient demonstrated a better eye quality and a wider optimum range of the GaAs top-layer thickness because the resultant higher damping reduced the relaxation oscillation.

• IMMUNE NETWORK
• /
• v.21 no.6
• /
• pp.42.1-42.20
• /
• 2021

Eosinophils play critical roles in the maintenance of homeostasis in innate and adaptive immunity. Although primarily known for their roles in parasitic infections and the development of Th2 cell responses, eosinophils also play complex roles in other immune responses ranging from anti-inflammation to defense against viral and bacterial infections. However, the contributions of pattern recognition receptors in general, and NOD-like receptors (NLRs) in particular, to eosinophil involvement in these immune responses remain relatively underappreciated. Our in vivo studies demonstrated that NLRC4 deficient mice had a decreased number of eosinophils and impaired Th2 responses after induction of an allergic airway disease model. Our in vitro data, utilizing human eosinophilic EoL-1 cells, suggested that TLR2 induction markedly induced pro-inflammatory responses and inflammasome forming NLRC4 and NLRP3. Moreover, activation by their specific ligands resulted in caspase-1 cleavage and mature IL-1β secretion. Interestingly, Th2 responses such as secretion of IL-5 and IL-13 decreased after transfection of EoL-1 cells with short interfering RNAs targeting human NLRC4. Specific induction of NLRC4 with PAM3CSK4 and flagellin upregulated the expression of IL-5 receptor and expression of Fc epsilon receptors (FcεR1α, FcεR2). Strikingly, activation of the NLRC4 inflammasome also promoted expression of the costimulatory receptor CD80 as well as expression of immunoregulatory receptors PD-L1 and Siglec-8. Concomitant with NLRC4 upregulation, we found an increase in expression and activation of matrix metalloproteinase (MMP)-9, but not MMP-2. Collectively, our results present new potential roles of NLRC4 in mediating a variety of eosinopilic functions.