• 반도체디스플레이기술학회지
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• 제5권3호
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• pp.37-41
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• 2006

In this paper, a composition modulation of Dot-matrix display grayscale is presented. There's two method of expression of Dot-matrix Display grayscale. One is PWM by controlling pulse width. The other is PAM by controlling pulse amplitude. Composition modulation of Dot-matrix use PWM and PAM together. Composition modulation shows the number of grayscale as number of grayscale of PWM$\times$number of grayscale of PAM and have the effect of reduced size than existing single modulation.

• Bulletin of the Korean Chemical Society
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• 제17권11호
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• pp.1036-1039
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• 1996

Synthetic lipoidal peptides based on viral protein sequences have been prepared. These peptides contain an N-palmitoyl group at the N-terminal residue, which is a modified cysteine, containing a S-[2,3-bis(acyloxy)-(2-R,S)-propyl] moiety. When this residue (Pam3Cys) is at the N-terminus of a synthetic peptide, it acts as potent immunoadjuvant to enhance both IgM and IgG antibody responses to the attached peptide. Conventional analytical procedures (e.g., Edman degradation and amino acid analysis) are either not applicable due to the N-terminal modification, or do not provide confirmation of the intact structure. Chromatographic analysis is also hindered by the tendency of these lipoidal Pam3Cys peptides to form large aggregates, and in some cases to be permanently adsorbed on reversed phase columns. We have applied several mass spectrometric techniques, including fast atom bombardment (FAB), electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI) to characterize the intact structures of a number of different Pam3Cys synthetic peptides. The MALDI-MS has been found to be the most sensitive for the analysis of the structure of Pam3Cys peptides.

• Interdisciplinary Bio Central
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• 제6권4호
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• pp.3.1-3.10
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• 2014

Amino acid substitution matrices are essential tools for protein sequence analysis, homology sequence search in protein databases and multiple sequence alignment. The PAM matrix was the first widely used amino acid substitution matrix. The BLOSUM series then succeeded the PAM matrix. Most substitution matrixes were developed by using the statistical frequency of substitution between each amino acid at blocks representing groups of protein families or related proteins. However, substitution of amino acids is based on the similarity of physiochemical properties of each amino acid. In this study, a new approach was used to obtain major physiochemical properties in multiple sequence alignment. Frequency of amino acid substitution in multiple sequence alignment database and selected attributes of amino acids in physiochemical properties database were merged. This merged data showed the major physiochemical properties through principle components analysis. Using factor analysis, these four principle components were interpreted as flexibility of electronic movement, polarity, negative charge and structural flexibility. Applying these four components, BAPS was constructed and validated for accuracy. When comparing receiver operated characteristic ($ROC_{50}$) values, BAPS scored slightly lower than BLOSUM and PAM. However, when evaluating for accuracy by comparing results from multiple sequence alignment with the structural alignment results of two test data sets with known three-dimensional structure in the homologous structure alignment database, the result of the test for BAPS was comparatively equivalent or better than results for prior matrices including PAM, Gonnet, Identity and Genetic code matrix.

• Bioinformatics and Biosystems
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• 제2권1호
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• pp.7-11
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• 2007

하나의 아미노산이 다른 아미노산으로 바뀌는 가능성을 계통학적인 나무를 이용해서 치환행렬로 만들었다. PFMT(Phylogenetic Focused Mutaion Tendency)행렬은 기존의 PAM160이나 BLOSUM62와 다르게 공통조상으로부터 상위 종으로 치환되는 가능성을 점수화 하였다. COGs의 데이터베이스에 있는 152KOGs를 뽑아서 아미노산의 치환횟수를 점수화하였다. PFMT 행렬은 어떤 서열보다 더 상위 종의 서열을 비교할 때 유용하게 쓰일 수 있으며 2개의 아미노산간의 치환 관계를 더 자세하게 볼 수 있게 한다.

• Bulletin of the Korean Chemical Society
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• 제35권4호
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• pp.1077-1081
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• 2014

Polyacrylamide (PAM) -HgS nanocomposites were successfully prepared in polyacrylamide (PAM) matrix. From TEM and XRD characterizations, the synthesized HgS nanocrystals were chain-like spherical in shape with a diameter of about 40-60 nm and high crystalline quality. The quantum-confined effect of HgS nanocrystals was confirmed by UV-vis diffuse reflection spectra. The optical properties of products were investigated by using photoluminescence (PL) spectra, which showed that HgS nanocrystals exhibited good optical properties with maximum emission peak at about 640 and 650 nm at different reaction temperatures. The interaction of HgS nanocrystals with PAM was studied through FT-IR spectroscopy and TG analysis, which suggested that $Hg^{2+}$ could interact with functional groups of PAM. The experimental results indicated that PAM not only induced nucleation, but also inhibited further growth of HgS crystals and play an important role in the formation of PAM/HgS nanocomposites. In addition, the possible mechanism of HgS nanoparticles growth in PAM solution was also discussed.

• 한국콘텐츠학회논문지
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• 제12권3호
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• pp.34-43
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• 2012

두 문자열 간의 유사도를 계산하는 문제는 정보 검색, 오타 교정, 스팸 필터링 등 다양한 분야에 응용될 수 있다. 동적 계획법 기반의 유사도 계산 방법을 통하여 한글 문자열의 유사도 계산을 위해서는 우선 음소간의 유사도에 대한 정의가 필요하다. 그러나 기존의 방법들은 수동적 설정에 의한 유사도 점수를 사용하고 있다는 한계점이 있다. 본 논문에서는 PAM(Point Accepted Mutation) 행렬과 유사한 확률 모델을 이용하여 변형 단어 집합으로부터 음소 간의 유사도를 자동적으로 계산하는 기법을 제안한다. 제안 기법은 주어진 변형 단어의 집합 내 유사한 단어 쌍을 찾아 문자열 정렬(Text Alignment)을 수행함으로써 음소 변형 규칙을 도출하고, 이로부터 각 음소 쌍의 상호 변형 빈도에 따른 유사도 점수를 계산한다. 실험 결과 특이도(Specificity) 77.2~80.4% 수준에서 불일치 여부에 따른 단순 점수 부여 방식에 비해서는 10.4~14.1%, 수동으로 음소 간 유사도를 직접 설정하는 방식에 비해서는 8.1~11.8%의 민감도(Sensitivity) 향상이 있음을 확인하였다.

• 폴리머
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• 제30권6호
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• pp.545-549
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• 2006

폴리(비닐 알코올) (PVA)과 폴리아크릴산-말레산-공중합체(PAM)의 블렌드를 수용액 상태로 얻은 후 점토인 사포나이트(SPT)를 분간시켜 필름 형태인 PVA/PAM/SPT의 나노복합재료를 합성하였다. 용액 삽입법을 이용하여 점토 함량을 0-9 wt%의 다양한 농도로 변화시켜 얻은 나노복합재료에 대해 분산도, 모폴로지 및 열적-기계적 성질 등을 각각 조사하였다. 점토 함량이 3 wt%일 때 점토 입자는 PVA/PAM 블렌드에 잘 분산되었으며, 점토함량이 7 wt%보다 많을 경우에는 고분자 모체에 일부 뭉친 구조가 관찰되었다. 나노복합재료의 열적 안정성은 점토함량이 9 wt%로 증가할 때까지 꾸준히 증가하였다. 인장 강도와 초기인장 탄성률은 점토 함량이 7 wt%일 때 최고값을 나타내었으나 그 이상의 점토 농도에서는 오히려 감소하였다. 본 연구 결과로부터 소량의 점토 첨가는 PVA/PAM 나노복합재료 필름의 열적, 기계적 성질을 증가시키는데 도움이 된다는 것을 알았다.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제3권2호
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• pp.187-193
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• 2003

In this paper, a systematic design method of the intelligent PAM fuzzy controller for nonlinear systems using the efficient tools-Linear Matrix Inequality and the intelligent digital redesign is proposed. In order to digitally control the nonlinear systems, the TS fuzzy model is used for fuzzy modeling of the given nonlinear system. The convex representation technique also can be utilized for obtaining TS fuzzy models. First, the analog fuzzy-model-based controller is designed such that the closed-loop system is globally asymptotically stable in the sense of Lyapunov stability criterion. The simulation results strongly convince us that the proposed method has great potential in the application to the industry.

• ETRI Journal
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• 제43권5호
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• pp.923-931
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• 2021

We studied the influence of GaAs top-layer thickness on the small-signal modulation response and 56 Gb/s four-level pulse-amplitude modulation eye quality of 850 nm vertical-cavity surface-emitting lasers (VCSELs). We considered the proportionality of the gain-saturation coefficient to the photon lifetime. The simulation results that employed the transfer-matrix method and laser rate equations led to the conclusion that the proportionality should be considered for proper explanation of the experimental results. From the obtained optical eyes, we could determine an optimum thickness of the GaAs top layer that rendered the best eye quality of VCSEL. We also compared two results: one result with a fixed gain-saturation coefficient and the other that considered the proportionality. The former result with the constant gain-saturation coefficient demonstrated a better eye quality and a wider optimum range of the GaAs top-layer thickness because the resultant higher damping reduced the relaxation oscillation.

• IMMUNE NETWORK
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• 제21권6호
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• pp.42.1-42.20
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• 2021

Eosinophils play critical roles in the maintenance of homeostasis in innate and adaptive immunity. Although primarily known for their roles in parasitic infections and the development of Th2 cell responses, eosinophils also play complex roles in other immune responses ranging from anti-inflammation to defense against viral and bacterial infections. However, the contributions of pattern recognition receptors in general, and NOD-like receptors (NLRs) in particular, to eosinophil involvement in these immune responses remain relatively underappreciated. Our in vivo studies demonstrated that NLRC4 deficient mice had a decreased number of eosinophils and impaired Th2 responses after induction of an allergic airway disease model. Our in vitro data, utilizing human eosinophilic EoL-1 cells, suggested that TLR2 induction markedly induced pro-inflammatory responses and inflammasome forming NLRC4 and NLRP3. Moreover, activation by their specific ligands resulted in caspase-1 cleavage and mature IL-1β secretion. Interestingly, Th2 responses such as secretion of IL-5 and IL-13 decreased after transfection of EoL-1 cells with short interfering RNAs targeting human NLRC4. Specific induction of NLRC4 with PAM3CSK4 and flagellin upregulated the expression of IL-5 receptor and expression of Fc epsilon receptors (FcεR1α, FcεR2). Strikingly, activation of the NLRC4 inflammasome also promoted expression of the costimulatory receptor CD80 as well as expression of immunoregulatory receptors PD-L1 and Siglec-8. Concomitant with NLRC4 upregulation, we found an increase in expression and activation of matrix metalloproteinase (MMP)-9, but not MMP-2. Collectively, our results present new potential roles of NLRC4 in mediating a variety of eosinopilic functions.