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Discrimination and Authentication of Eclipta prostrata and E. alba Based on the Complete Chloroplast Genomes

  • Kim, Inseo;Park, Jee Young;Lee, Yun Sun;Lee, Hyun Oh;Park, Hyun-Seung;Jayakodi, Murukarthick;Waminal, Nomar Espinosa;Kang, Jung Hwa;Lee, Taek Joo;Sung, Sang Hyun;Kim, Kyu Yeob;Yang, Tae-Jin
    • Plant Breeding and Biotechnology
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    • v.5 no.4
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    • pp.334-343
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    • 2017
  • Eclipta prostrata and E. alba are annual herbal medicinal plants and have been used as Chinese medicinal tonics. Both species are widely distributed in tropical and subtropical regions as well as in Korea. Both species have similar morphological features but E. alba has smoother leaf blade margins compared with E. prostrata. Although both species are utilized as oriental medicines, E. prostrata is more widely used than E. alba. Morphological semblances have confounded identification of either species. Here, we report the complete chloroplast genomes of both species to provide an authentication system between the two species and understand their diversity. Both chloroplast genomes were 151,733-151,757 bp long and composed of a large single copy (83,285-83,300 bp), a small single copy (18,283-18,346 bp), and a pair of inverted repeats (25,075-25,063 bp). Gene annotation revealed 80 protein coding genes, 30 tRNA genes and four rRNA genes. A phylogenetic analysis revealed that the genus Eclipta is grouped with Heliantheae tribe species in the Asteraceae family. A comparative analysis verified 29 InDels and 58 SNPs between chloroplast genomes of E. prostrata and E. alba. The low chloroplast genome sequence diversity indicates that both species are really close to each other and are not completely diverged yet. We developed six DNA markers that distinguish E. prostrata and E. alba based on the polymorphisms of chloroplast genomes between E. prostrata and E. alba. The chloroplast genome sequences and the molecular markers generated in this study will be useful for further research of Eclipta species and accurate classification of medicinal herbs.

Optimization of Solar Water Battery for Efficient Photoelectrochemical Solar Energy Conversion and Storage (효율적인 광전기화학적 태양에너지 전환과 저장을 위한 Solar Water Battery의 최적화)

  • Go, Hyunju;Park, Yiseul
    • Clean Technology
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    • v.27 no.1
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    • pp.85-92
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    • 2021
  • A solar water battery is a system that generates power using solar energy. It is a combination of photoelectrochemical cells and an energy storage system. It can simultaneously convert and store solar energy without additional external voltage. Solar water batteries consist of photoelectrodes, storage electrodes and counter electrodes, and their properties and combination are important for the performance and the efficiency of the system. In this study, we tried to find the effect that changing the components of solar water batteries has on its system. The effects of the counter electrode during discharge, the kinds of photoelectrode and storage electrode materials, and electrolytes on the solar energy conversion and storage capacitance were studied. The optimized composition (TiO2 : NaFe-PB : Pt foil) exhibited 72.393 mAh g-1 of discharge capacity after 15 h of photocharging. It indicates that the efficiency of solar energy conversion and storage is largely affected by the configuration of the system. Also, the addition of organic pollutants to the chamber of the photoelectrode improved the battery's photo-current and discharge capacity by efficient photoelectron-hole pair separation with simultaneous degradation of organic pollutants. Solar water batteries are a new eco-friendly solar energy conversion and storage system that does not require additional external voltages. It is also expected to be used for water treatment that utilizes solar energy.

Rice Grain Damage by Paromius exiguus (Distant)(Heteroptera: Lygaeidae) in Rice Fields (흑다리긴노린재 [Paromius exiguus (Distant)](Heteroptera: Lygaeidae)에 의한 벼 이삭 피해)

  • Seo, Bo Yoon;Cho, Jum Rae;Lee, Si-Woo;Kim, Hey-Kyung;Park, Chang-Gyu
    • Korean journal of applied entomology
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    • v.59 no.4
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    • pp.455-460
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    • 2020
  • Rice grain damage caused by Paromius exiguus under various conditions in paddy fields was analyzed. One nymph of P. exiguus caused 2.7 pecky grains in brown rice in one day, and sixteen nymphs caused 132.3 pecky grains over sixteen days. The relationship between the inoculated nymphal density and the number of pecky grains was well described by a linear regression (Y = 8.8446X, r2 = 0.9622). When a pair of P. exiguus adults was inoculated in the heading stage of rice, the damage at the time of harvesting consisted of 40.2% of cracked rice and 30.5% of pecky rice among whole rice grains. Inoculation of 16 pairs resulted in 81.7% of cracked rice and 74.5% of pecky rice. The damage analysis indicated that the quantity of ripened rice decreased as the damage started earlier in the heading stage of rice. On the contrary, the proportion of cracked and pecky rice rapidly increased from the heading time until 28 days after heading of the rice grains.

Conservation Treatment of Leather Socks Housed in the National Museum of Korea (국립중앙박물관 소장 가죽버선의 보존처리)

  • Lee, Hyelin;Park, Seungwon
    • Conservation Science in Museum
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    • v.27
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    • pp.39-56
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    • 2022
  • The purpose of this project was to improve the stability of a pair of leather socks for use on snowy days in the collection of the National Museum of Korea (Namsan1567) by conducting conservation treatment and restoring the socks to their original form for use in research and exhibition. Leather socks are referred to in ancient documents with names combining the word "mal" for socks with a term indicating their material (e.g., pimal, meaning leather socks; nokpimal, meaning deer leather socks; jangpimal, meaning roe deer leather socks, or lipimal, meaning racoon dog leather socks) and are mentioned mostly in connection with Jeju Island. Related documents include the Seungjeongwon ilgi (Daily Record of the Grand Secretariat), Injaeilnok (Diary of Injae Jo Geukseon), and Hamel's Journal and a Description of the Kingdom of Joseon. Extant examples of ancient leather socks display the same form as beoseon (traditional Korean socks) and are made of either leather or a combination of leather and fabric. It is likely that such leather socks were worn on Jeju Island to protect the feet from the cold. A condition survey of the leather socks was first conducted to establish a plan for their conservation treatment. Since the socks were in rather poor condition, it was decided to identify their original form through an investigation of relics and pertinent previous studies. The socks were cleaned in consideration of results of the condition survey, and the missing parts around the necks of the socks were reinforced in a reversible manner using counting stitches with cloth dyed to match the original color. Since the bottoms of the socks had lost much of their original form due to deterioration and disintegration in the leather, supports were made and inserted inside the socks to help retain their shape. Through these processes, the structure and characteristics of the socks and the techniques used in their production could be analyzed, their condition was stabilized, and their original form was recovered.

Reduction of VOCs and the Antibacterial Effect of a Visible-Light Responsive Polydopamine (PDA) Layer-TiO2 on Glass Fiber Fabric (Polydopamine (PDA)-TiO2 코팅 유리섬유 직물을 이용한 VOCs의 저감 성능 및 항균성 연구)

  • Park, Seo-Hyun;Choi, Yein;Lee, Hong Joo;Park, Chan-gyu
    • Journal of Environmental Health Sciences
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    • v.47 no.6
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    • pp.540-547
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    • 2021
  • Background: Indoor air pollutants are caused by a number of factors, such as coming in from the outside or being generated by internal activities. Typical indoor air pollutants include nitrogen dioxide and carbon monoxide from household items such as heating appliances and volatile organic compounds from building materials. In addition there is carbon dioxide from human breathing and bacteria from speaking, coughing, and sneezing. Objectives: According to recent research results, most indoor air pollution is known to be greatly affected by internal factors such as burning (biomass for cooking) and various pollutants. These pollutants can have a fatal effect on the human body due to a lack of ventilation facilities. Methods: We fabricated a polydopamine (PDA) layer with Ti substrates as a coating on supported glass fiber fabric to enhance its photo-activity. The PDA layer with TiO2 was covalently attached to glass fiber fabric using the drop-casting method. The roughness and functional groups of the surface of the Ti substrate/PDA coated glass fiber fabric were verified through infrared imaging microscopy and field emission scanning electron microscopy (FE-SEM). The obtained hybrid Ti substrate/PDA coated glass fiber fabric was investigated for photocatalytic activity by the removal of ammonia and an epidermal Staphylococcus aureus reduction test with lamp (250 nm, 405 nm wavelength) at 24℃. Results: Antibacterial properties were found to reduce epidermal staphylococcus aureus in the Ti substrate/PDA coated glass fiber fabric under 405 nm after three hours. In addition, the Ti substrate/PDA coated glass fiber fabric of VOC reduction rate for ammonia was 50% under 405 nm after 30 min. Conclusions: An electron-hole pair due to photoexcitation is generated in the PDA layer and transferred to the conduction band of TiO2. This generates a superoxide radical that degrades ammonia and removes epidermal Staphylococcus aureus.

Analysis of Educational Elements of Educational Online Games - Focused on Real Farm Games - (교육용 온라인 게임의 교육적 요소 분석 - 레알팜 게임을 중심으로 -)

  • Jung, Mi-A;Jung, Hyung-Won
    • Journal of Korea Entertainment Industry Association
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    • v.14 no.3
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    • pp.173-180
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    • 2020
  • According to KISA, many people play smartphone games. stress relieving, time is the reason to beat. Attempts to combine mobile games and education in the Korean game market are continuing. Studies have shown that using commercial games for education can be effective. Also, researches for activation educational games are increasing. Creating educational games requires a lot of time and money. Due to the nature of mobile games, the service is often terminated without updating. If you are able to acquire a naturally educational part while playing mobile games, you will see the effect of one pair of fun and education, both at once. We analyzed the education factors of the game with 'Real Farm' which is a simulation farm game. We focused on analyzing information acquisition and utilization part through indirect experience which is characteristic of simulation and examined educational factors based on design elements of educational game. This can signal the positive aspects of the game and the educational content of existing games. If you use smartphones as a pedagogy, you can expect a positive perception of games and an increase in the average life expectancy of mobile games.

Development of TDR-based Water Leak Detection Sensor for Seawater Pipeline of Ship (시간영역반사계를 이용한 해수배관시스템의 누수 탐지용 센서 개발 연구)

  • Hwang, Hyun-Kyu;Shin, Dong-Ho;Kim, Heon-Hui;Lee, Jung-Hyung
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.6
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    • pp.1044-1053
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    • 2022
  • Time domain reflectometry (TDR) is a diagnostic technique to evaluate the physical integrity of cable and finds application in leak detection and localization of piping system. In this study, a cable-shaped leak detection sensor was proposed using the TDR technique for monitoring leakage detection of ship's engine room seawater piping system. The cable sensor was developed using a twisted pair arrangement and wound by an absorbent material. The availability and performance of the sensor for leak detection and localization were evaluated on a lab-scale pipeline set up. The developed sensor was installed onto the pipes and flanges of the lab-scale set up and various TDR waveforms were acquired and analyzed according to the dif erent variables including the number of twists and sheath thickness. The result indicated that the twisted cable sensor was able to produce clear and smooth signal as compared to the TDR sensor with a parallel arrangement. The optimal number of twist was determined to be above 10 per the unit length. The optimal diameter of sheath thickness that results in the desired sensitivity was determined to be ranging from 80% up to 120% of the diameter of the conductor. The linear regression analysis for estimation of leak localization was carried out to estimate the location of the leakage, and the result was a determination coefficient of 0.9998, indicating a positive relationship with the actual leakage point. The proposed TDR based leak detection method appears to be an effective method for monitoring leakage of ship's seawater piping system.

Dietary Iron Intake of Koreans Estimated using 2 Different Sources of Iron Contents are Comparable: Food & Nutrient Database and Iron Contents of Cooked Foods in the Korean Total Diet Study (우리 국민의 철 섭취량 평가: 식품별 영양성분 함량자료와 한국형 총식이조사 기반 추정량 비교)

  • Lee, Jeeyeon;Kwon, Sung Ok;Yeoh, Yoonjae;Seo, Min Jeong;Lee, Gae Ho;Kim, Cho-il
    • Korean Journal of Community Nutrition
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    • v.27 no.3
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    • pp.245-253
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    • 2022
  • Objectives: This study was conducted to find out if the dietary iron intake of Koreans estimated by 2 different methods (iron content sources) using the food intake data from the Korea National Health and Nutrition Examination Survey (KNHANES) are comparable. One method was based on the KNHANES's Food & Nutrient Database (FND) derived mainly from the Korean Food Composition Table and the other used the iron content (IC) of food samples processed in the Korean Total Diet Study (KTDS). Methods: Dietary intake data from the 2013-2016 KNHANES was used to select representative foods (RFs) in KTDS for iron analysis. Selection of the RFs and cooking methods for each RF (RF × cooking method pair) was performed according to the 'Guidebook for Korean Total Diet Studies' and resulted in a total of 132 RFs and 224 'RF × cooking method' pairs. RFs were collected in 9 metropolitan cities nationwide once or twice (for those with seasonality) in 2018 and made into 6 composites each, based on the origin and season prior to cooking. Then, the RF composites prepared to a 'table ready' state for KTDS were analyzed by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Dietary iron intake of the Korean population was estimated using only RFs' intake data based on the 2 sources of iron content, namely FND-KNHANES and IC-KTDS. Results: RFs in KTDS covered 92.0% of total food intake of Koreans in the 2016-2018 KNHANES. Mean iron intake of Koreans was 7.77 mg/person/day by IC-KTDS vs 9.73 mg/person/day by FND-KNHANES. The major food groups contributing to iron intake were meats (21.7%), vegetables (20.5%), and grains & cereals (13.4%) as per IC-KTDS. On the other hand, the latter source (FND-KNHANES) resulted in a very different profile: grains & cereals (31.1%), vegetables (16.8%), and meats (15.3%). While the top iron source was beef, accounting for 8.6% in the former, it was polished rice (19.2%) in the latter. There was a 10-fold difference in the iron content of polished rice between 2 sources that iron intakes excluding the contribution by polished rice resulted in very similar values: 7.58 mg/person/day by IC-KTDS and 7.86 mg/person/day by FND-KNHANES. Conclusions: This study revealed that the dietary iron intake estimated by 2 different methods were quite comparable, excluding one RF, namely polished rice. KTDS was thus proven to be a useful tool in estimating a 'closer-to-real' dietary intake of nutrients for Koreans and further research on various nutrients is warranted.

Interpretation of Construction Procedure and Physicochemical Characteristics for Soil Layers from Sowangneung (Small Royal Tomb) of Ssangneung (Twin Tombs) in Iksan, Korea (익산 쌍릉 소왕릉 봉분 토층의 물리화학적 특성과 조영과정 해석)

  • Chae, Joon;Park, Seok Tae;Cho, Ji Hyun;Lee, Chan Hee
    • Journal of Conservation Science
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    • v.37 no.6
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    • pp.748-766
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    • 2021
  • The Iksan Ssangneung (twin tombs), a pair of tombs comprising the Daewangneung (large royal tomb) and the Sowangneung (small royal tomb), were constructed in the typical style of stone tunnel and chamber tombs in the Baekje Kingdom during the Sabi period (538 to 660 AD) of ancient Korea. Soil layers exposed during excavation of Sowangneung in a trench east of the tomb are: the bottommost layer, the ground level layer, the Panchuk (rammed earth) layer of the Baekje, the layer created by a grave robbery, and soil recovered during the Japanese colonial period. Soil samples were obtained by segmenting an easy stratigraphic horizon into sub categorized soil layers, and their material properties were analyzed; they are composed mainly of sandy loam based on the particle size distributions. In the site foundation, loamy sand is packed in the bottommost layer, and sandy loam with high sand and silty sand fills most of the overlying layer. The central and topmost portion of the Baekje layer is composed of loam with high clay content. All soil layers show geochemical behaviors similar to those of the bottommost layer. X-ray diffraction analysis verified kaolinite in all layers, also observed in soil layers displaying high crystallinity. Kaolinite and halloysite were identified by scanning electron microscopy. Thus, we conclude that the Baekje layer of the Sowangneung is composed of sandy loam containing kaolin procured from near the site. An impermeable middle to upper layer was created using viscous loam. The top of the tomb was closed tightly.

Effect of Distance between Finger Tip and Root Width on Compressive Strength Performance of Finger-Jointed Timber (핑거공차가 핑거접합재의 압축강도 성능에 미치는 영향)

  • Ryu, Hyun-Soo;Ahn, Sang-Yeol;Park, Han-Min;Byeon, Hee-Seop;Kim, Jong-Man
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.4
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    • pp.66-73
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    • 2004
  • Three species of Italian poplar (Populus euramericana), red pine (Pinus densiflora) and oriental oak (Quercus variabilis) were selected for this study. They were cut so that the distances between each of tips and roots for a pair of fingers were 0, 0.15, 0.30 and 0.45 mm. Poly vinyl acetate (PVAc) and resorcinol-phenol resin (RPR) were used for finger-jointing. Compressive test parallel to the grain was conducted for the finger-jointed specimens. The results were as follows: The efficiency of compressive Young's modulus of finger-jointed timber to solid wood indicated low values, whereas the efficiency of compressive strength indicated high values of more than 90% in all species, especially, it was found that those of red pine indicated markedly high values of more than 97%. The efficiency of compressive displacement of Italian poplar finger-jointed timber was 2 times higher than solid wood, and it was 1.2 and 1.3 times higher than solid woods in red pine and oriental oak, respectively. Also, it was found that 0, the distance between each tip and root for the fingers, indicated the highest efficiency of compressive strength performance in Italian poplar finger-jointed timber, and for red pine and oriental oak finger-jointed timbers, the distances of 0.15 and 0.30 were found to indicate the highest efficiency.