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Recycling of Fermented Sawdust-based Oyster Mushroom Spent Substrate as a Feed Supplement for Postweaning Calves

  • Kim, Min-Kook;Lee, Hong-Gu;Park, Jeong-Ah;Kang, Sang-Kee;Choi, Yun-Jaie
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.4
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    • pp.493-499
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    • 2011
  • The objective of this study was to find the way to prolong the storage time of sawdust-based oyster mushroom (Pleurotus osteratus) spent substrate (OMSS) by fermenting with potential probiotic microorganisms to recycle the otherwise waste of mushroom farms. To this purpose, lactic acid bacteria (LAB) were screened to select the best lactic acid-producing strains. Three strains of LAB (Lactobacillus plantarum Lp1', Pediococcus acidilacticii Pa193, L. plantarum Lp2M) were selected and in mixture they lowered the pH of the fermented OMSS to 3.81. fOMSS (fermented sawdust-based oyster mushroom spent substrate) could be stored at room temperature for at least 17 days without any deterioration of feed quality based on the pH, smell, and color. In dry matter disappearance rate in situ, commercial TMR (total mixed ration), OMSS and OMMM (oyster mushroom mycelium mass) showed no significant differences between the samples after 6, 12 and 24 h incubation except for 48 h. Two separate field studies were performed to test the effects of fOMSS supplement on the growth performance of postweaning Holstein calves. Field trials included groups of animals feeding calf starter supplemented with: Control (no supplement), AB (colistin 0.08% and oxyneo 110/110 0.1%), fOMSS (10% fOMSS) and fConc (10% fermented concentrate) and DFM (direct-fed microbials, average $10^9$ cfu for each of three LAB/d/head). Growth performance (average daily gain and feed efficiency) of the fOMSS supplement group was higher than that of AB followed by fConc and DFM even though there was no statistically significant difference. The Control group was lower than any other group. Various hematological values including IgG, IgA, RBC (red blood cell), hemoglobin, and hematocrit were measured every 10 days to check any unusual abnormality for all groups in trial I and II, and they were within a normal and safe range. Our results suggest that sawdust-based OMSS could be recycled after fermentation with three probiotic LAB strains as a feed supplement for post-weaning calves, and fOMSS has the beneficial effects of an alternative to antibiotics for a growth enhancer in dairy calves.

Assessment of Food Service Management Practices in Child Care Centers Operated by Various Types of Foundations in the Chonbuk Area of Korea (전북지역 보육시설의 시설유형에 따른 급식운영관리 실태 조사)

  • Rho, Jeong-Ok;Lee, Eun-Pa;Lee, Jin-Sook
    • Korean journal of food and cookery science
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    • v.25 no.1
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    • pp.74-83
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    • 2009
  • We investigated the food service management practices in child care centers operated by various types of foundations in the Chonbuk area of Korea. Self-administered questionnaires were collected from 125 child care center directors. The statistical analysis was completed using an SPSS v11.5 program. Approximately 82.4% of the directors were women with an the average age of 45 years old. The average number of children in each public and private child care center was 84.7 and 88.8, respectively (P<.001), and the difference in time of operation was significant(P<.001). Only 20.8% of the centers employed a dietitian, whereas 92.0% of the centers employed a cook, thus, food service was not managed by professionals in most centers. In approximately 99.2% of the centers, meals were prepared in a conventional manner. The difference in daily meal frequency was significant(P<.05), with approximately 85.6% of the centers serving snacks twice a day primarily to supplement the intake of nutrients(4.44 point) and to add fun to the daily lives of the children(4.12 point). Approximately 40.7% of the directors of public centers and 57.6% of the directors of private centers responded that the most difficult aspect of food service management was financial management. Overall, 56.8% of the directors responded that the details of financial management had the greatest effect on their ability to improve the quality of food service. In most centers, the center director was also the purchasing manager and half of them purchased food every day. Approximately 97% of the directors responded that they do not include instant foods in their menu plans but they would use them if the children wanted to eat them. These results indicate that the food service management in child care centers in the Chonbuk area is relatively inadequate. We strongly recommend that they hire dietitians for food service administration and focus on strengthening sanitation management.

A Study of Measurement on Airtightness and Air-Flow Performance of Apartment Housing Adopting Window Frame-Type Natural Ventilation (자연환기장치가 적용된 공동주택의 기밀 및 공기유동 성능 실측 연구)

  • Chun, Chu-Young;Kim, Gil-Tae;Kim, Sun-Dong
    • Land and Housing Review
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    • v.5 no.4
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    • pp.325-332
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    • 2014
  • The purpose of this study was to measure the airtightness and Air-Flow Performance for 7th house of small apartment houses adopted window frame-type natural ventilation. All window and living room door is provide with window frame-type natural ventilation, and there is provide with manual controller. As the object of measurement, the 6th type small apartment houses with area of $33m^2$ to $51m^2$ was selected. airtightness performance was measured at the front door using Blower door system. We measured ventilation rate per hour on 50Pa pressure different between inside and outside by the 1st to 6th cases. As a result, when the natural ventilation frame was closed, average amounts are shown as the ventilation rate per hour were 2.27ACH (CASE1). and the result is similar to general apartment house (1.65~4.28ACH). When the natural ventilation frame was open, average amounts are shown as the ventilation rate per hour were 5.87ACH (CASE6). In addition, that's a 3.6ACH increased more than CASE1.

Single-crystal Structure of Partially Dehydrated Partially Mg2+-exchanged Zeolite Y (FAU), |Mg30.5Na14(H2O)2.5|[Si117Al75O384]-FAU

  • Kim, Hu-Sik;Ko, Seong-Oon;Lim, Woo-Taik
    • Bulletin of the Korean Chemical Society
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    • v.32 no.10
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    • pp.3696-3701
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    • 2011
  • The single-crystal structure of partially dehydrated partially $Mg^{2+}$-exchanged zeolite Y, ${\mid}Mg{30.5}Na_{14}(H_2O)_{2.5}{\mid}$ [$Si_{117}Al_{75}O_{384}$]-FAU per unit cell, ${\alpha}$ = 25.5060(1) ${\AA}$, dehydrated at 723 K and $1{\times}10^{-4}$ Pa, has been determined by single-crystal X-ray diffraction techniques in the cubic space group Fd$\bar{3}$ m at 100(1) K. The structure was refined using all intensities to the final error indices (using only the 561 reflections with $F_{\circ}$ > $4{\sigma}(F_{\circ})$) $R_1$ = 0.0377 (Based on F) and $R_2$ = 0.1032 (Based on $F^2$). About 30.5 $Mg^{2+}$ ions per unit cell are found at four different crystallographic sites. The 14 $Mg^{2+}$ ions occupy at site I at the center of double 6-ring (Mg-O = 2.231(3) ${\AA}$, O-Mg-O = $89.15(11)^{\circ}$ and $90.85(11)^{\circ}$). Four $Mg^{2+}$ ions are found at site I' in the sodalite cavity; the $Mg^{2+}$ ions are recessed 1.22 ${\AA}$ into the sodalite cavity from their 3-oxygen plane (Mg-O = 2.20(3) ${\AA}$ and O-Mg-O = $92.3(14)^{\circ}$). Site II' positions (opposite single 6-rings in the sodalite cage) are occupied by 2.5 $Mg^{2+}$ ions, each coordinated to an $H_2O$ molecule (Mg-O = 2.187(20) ${\AA}$ and O-Mg-O = $114.2(16)^{\circ}$). The 10 $Mg^{2+}$ ions are nearly three-quarters filled at site II in the supercage, being recessed 0.12 ${\AA}$ into the supercage (Mg-O = 2.123(4) A and O-Mg-O = $119.70(19)^{\circ}$). About 14 $Na^+$ ions per unit cell are found at one crystallographic site; the $Na^+$ ions are located at site II in the supercage (Na-O = 2.234(7) ${\AA}$ and O-Mg-O = $110.5(4)^{\circ}$).

Effect of $\gamma$-Irradiation of Volatile Organic Compounds of Houttuynia cordata Thunb. (방사선 조사에 의한 어성초의 휘발성 유기성분 변화)

  • Ryu, Keun-Young;Shim, Sung-Lye;Jung, Min-Seok;Jun, Sam-Nyeo;Jo, Cheor-Un;Song, Hyun-Pa;Kim, Kyong-Su;Kim, Youn-Soon
    • Food Science and Preservation
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    • v.15 no.3
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    • pp.411-420
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    • 2008
  • This study was performed to examine the effect of $\gamma$-irradiation on the volatile organic compounds in Houttuynia cordata Thunb.(H. cordata). 1be volatile compounds of non-irradiated and 10 kGy $\gamma$-irradiated H. cordata were isolated using SDE apparatus and analyzed by GC/MS. For each treatment the number of volatile compounds were detected at 83 and 85, respectively, 1be predominant functional groups of volatile organic compounds from H. cordata were identified as alcohols and ketones. H. cordata was mainly composed of hexahydrofarnesyl acetone (12.81 %), phytol, decanoic acid, dodecanoic acid, octadecanol, caryophyllene oxide, 2-undecanone and menthol. Houttuynum which is characteristic compound of H. cordata was found in all samples and there was no significant difference with irradiation doses. Consequently, $\gamma$-irradiation of H. cordata would be an effective process for sanitation and to increase extraction efficiency.

Catalytic CVD-Kinetics of Pyrolytic Carbon and SiC on the Stainless Steel Stent (Stainless Steel Stent에 Pyrolytic Carbon과 SiC의 촉매적 CVD-Kinetic연구)

  • 이보성;이무용
    • Proceedings of the KAIS Fall Conference
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    • 2000.10a
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    • pp.30-33
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    • 2000
  • 최근 국내에서도 관 동맥 질환 환자의 수가 급증하고 있으며, 관 동맥 질환의 치료 방법인 관 동맥 성형 술은 관 동맥 stent의 도입에 의하여 보편화되어 국내에서 년간 5000개 이상의 stent가 시술되고 있다. 그러나 stent는 고가(1,200천원/개)로 전량 수입에 의존하고 있으며, 시술 후 사망까지 이를 수 있는 혈전에 의한 급성 페쇠와 재 협착이 문제점이다. 이를 위한 한가지 방법이 생체 적합성이 뛰어난 복합 stent의 개발인데 SiC나 Carbon을 coating한 stent는 시술 후 혈전 형성을 억제하는 것으로 알려져 있다. 특히 가장 순수한 Pyrolytic carbon은 hemocompatibility가 탁월하고 기밀 성이기 때문에 본 연구에서 그의 CVB-Kinetics를 연구코저 하는 것이다. methane으로부터 pyrolytic carbon의 CVD는 온도에 따라서 다양한 구조를 가지며 따라서 그의 mechanism도 다양하다는 것은 잘 알려져 있다. 더구나 광간(균질)반응과 표면(불균질)반응의 정량적 관계에 따라서도 다르다는 것도 확인되었다. 그러나 stainless steel 316L로 만든 stent는 12 - 15 %의 Ni과 2%의 Mo을 함유해서 금속성을 잃지 않는 저온(600℃)에서도 pyrolytic carbon의 속매적 CVD가 가능함을 그리고 SiC의 코팅에 적합한 buffer layer 역할을 함을 확인하였다. 그리하여 본 연구는 반응기 설계에 필요한 저온 촉매적 pyrolytic carbon의 CVD-kinetics의 연구결로 그의 mechanism과 함께 rate law 식을 유도, 확인하였으며 600℃, 90kPa에서 P/sub ch4//P/sub H2/=5:1과 체류시간 1.8 sec가 최적임을 발견하였다. 이때 석출속도 11.2 g-mol/g-cat.h 혹은 두께속도로 73 nm/sec를 나타내었다.메타놀-물 (1 : 1) 유출액에서 $(0.80\;{\mu}g)$ 검출되었다. 하면 morey eel내장에서 얻은 독물질도 DEAE-셀루로즈에서 ST-1 과 ST-2로 나누어지며, 이 ST-1의 TLC, HPLC 및 알루미나 컬럼상의 거동이 파랑비늘돔에서 얻은 ST-1의 그것과 같으므로 scaritoxin으로 보고한 ST-1은 ciguatoxin의 형태인 less polar cigutoxin (LPCTX) 으로 생각된다.에서 각각 대조구의 57, 413 및 315% 증진되었다. 거품의 열안정성은 15분 whipping시, pH 4.0(대조구, 30.2%) 및 5.0(대조구, 23.7%)에서 각각 $0{\sim}38.0$$0{\sim}57.0%$이었고 pH 7.0(대조구, 39.6%) 및 8.0(대조구, 43.6%)에서 각각 $0{\sim}59.4$$36.6{\sim}58.4%$이었으며 sodium alginate 첨가시가 가장 양호하였다. 전체적으로 보아 거품안정성이 높은 것은 열안정성도 높은 경향이며, 표면장력이 낮으면 거품형성능이 높아지고, 비점도가 높으면 거품안정성 및 열안정성이 높아지는 경향이 있었다.protocol.eractions between application agents that are developed using different languages. Dynamic agent invocation is accomplished by Java Native Interface(JNI) that links two heterogeneous methods, and by KQML language interface that facilitates the communications between heterogeneous agents. This scheme of dyna

Media Expression and Structure Generation under RTSP for Effective Transmission on Mobile Environment with PoC Box system (PoC BoX시스템이 적용된 모바일 환경에서 단말로의 효율적인 전송을 위한 RTSP 기반 미디어 표현 및 구조 생성 방법)

  • Lee, Sung-Jun;Kim, Dae-Won
    • Journal of Korea Multimedia Society
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    • v.12 no.8
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    • pp.1142-1154
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    • 2009
  • The brand new type of mobile terminal services are kept being introduced in accordance with the development of mobile communication technology. Among many kinds of mobile application services, the PoC application standard which is using instant messaging service and group calls method with the existing walkie-talkie technology has been finished as the version 1.0 after tremendously active discussion and is being continued to be confirmed as 2.0 and 2.1. The PoC Box, which is discussed for replacing the PoC client and intermediate object as a voice messaging box, is currently being introduced and the biggest issues for PoC Box technology topics include the part of saved informations' processing and effective multimedia contents' transmission in the PoC Box system. In this research, we propose that the PaC client could effectively transmit the media to the end-user by specifying the playback location or range, focusing on the contents and the methods of dynamic controlling for saved media in PoC Box. This paper deals with the way of dynamic controlling method using the RTSP which is appropriate for PoC Box and the effective method for generation, expression, processing of various multimedia contents including audio and video objects.

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Interpretation of Blood Gas Analysis During Hypothermic Cardiopulmonary Bypass (저체온 체외순환시 혈액가스분석의 판독과 체온과의 상관관계)

  • Song, Sun-Ok
    • Journal of Yeungnam Medical Science
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    • v.6 no.1
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    • pp.121-131
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    • 1989
  • The temperature-corrected values of blood gas analysis were compared to uncorrected values in 40 cases of open heart surgery under moderate hypothermic cardiopulmonary bypass. The results were as follows. 1. The corrected value of pH was significantly higher than uncorrected value, and it's relationship was ${\Delta}pH=-0.015$ ${\Delta}Temp+0.005$(r=0.81, P<(0.01). 2. The corrected value of $PCO_2$ was lower than uncorrected value, and it's relationship was ${\Delta}PCO_2=1.11$ ${\Delta}Temp+1.81$(r=0.50, P<0.01). 3. The corrected value of $PO_2$ was lower than uncorrected value, and it' s relationship was ${\Delta}PO_2=5.21$ ${\Delta}Temp-1.45$(r=0.32, P<0.01). But there was no clinical significance. 4. The corrected values of $HCO_{3^-}$, base excess, $CO_2$ content and oxygen saturation were similar with uncorrected values. In summery, the values of pH and $PCO_2$were significantly changed by temperature-correction. Because of the neutral point of water (pH=pOH) rises as temperature falls and it change in parallel with the changes in blood pH, a corrected pH of 7.4, $PaCO_2$ of 40mmHg during deep hypothermia would reflect a profound respiratory acidosis. Therefore, the use of the uncorrected value at $37^{\circ}C$ is more preferable and valid means of assessing acid-base management regardless of actual patient temperature.

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Microbiological Contamination Level and Radiation Sterilization in Disposable Kitchen Utensil (일회용 주방용품의 미생물 오염도평가 및 방사선 살균)

  • Kim Jin-Hee;Lim Sang-Yong;Song Hyun-Pa;Kim Byeong-Keun;Chung Jin-Woo;Yoon Hae-Jung;Byun Myung-Woo;Kim Dong-Ho
    • Food Science and Preservation
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    • v.12 no.4
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    • pp.317-322
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    • 2005
  • The purposes of this study were to assess the microbiological contamination level of various disposable kitchen utensils and evaluate the effectiveness of gamma irradiation as a sterilization process for the utensils. The 51 kinds in 17 groups of disposable kitchen utensils were tested for the enumeration of total aerobic plate count, coliforms, fungi and Salmonella. Generally, cell density of microorganisms in disposable kitchen utensils were lower than that of cooking utensils currently using in kitchen. The survivals of total aerobic plate counts, putrefactive bacteria, on the surface of the disposable utensils were ranged up to $10^3\;CFU/100\;cm^2$. Filamentous fungi were detected in 13 samples. Coliform bacteria were detected in two kinds of samples but Salmonella spp. was detected below detection limits in all the samples. The microorganisms survived on the surface of utensils were effectively eliminated by gamma irradiation process at 3 kGy.

Genome characterization and mutation analysis of human influenza A virus in Thailand

  • Rattanaburi, Somruthai;Sawaswong, Vorthon;Nimsamer, Pattaraporn;Mayuramart, Oraphan;Sivapornnukul, Pavaret;Khamwut, Ariya;Chanchaem, Prangwalai;Kongnomnan, Kritsada;Suntronwong, Nungruthai;Poovorawan, Yong;Payungporn, Sunchai
    • Genomics & Informatics
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    • v.20 no.2
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    • pp.21.1-21.14
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    • 2022
  • The influenza A viruses have high mutation rates and cause a serious health problem worldwide. Therefore, this study focused on genome characterization of the viruses isolated from Thai patients based on the next-generation sequencing technology. The nasal swabs were collected from patients with influenza-like illness in Thailand during 2017-2018. Then, the influenza A viruses were detected by reverse transcription-quantitative polymerase chain reaction and isolated by MDCK cells. The viral genomes were amplified and sequenced by Illumina MiSeq platform. Whole genome sequences were used for characterization, phylogenetic construction, mutation analysis and nucleotide diversity of the viruses. The result revealed that 90 samples were positive for the viruses including 44 of A/H1N1 and 46 of A/H3N2. Among these, 43 samples were successfully isolated and then the viral genomes of 25 samples were completely amplified. Finally, 17 whole genomes of the viruses (A/H1N1, n=12 and A/H3N2, n=5) were successfully sequenced with an average of 232,578 mapped reads and 1,720 genome coverage per sample. Phylogenetic analysis demonstrated that the A/H1N1 viruses were distinguishable from the recommended vaccine strains. However, the A/H3N2 viruses from this study were closely related to the recommended vaccine strains. The nonsynonymous mutations were found in all genes of both viruses, especially in hemagglutinin (HA) and neuraminidase (NA) genes. The nucleotide diversity analysis revealed negative selection in the PB1, PA, HA, and NA genes of the A/H1N1 viruses. High-throughput data in this study allow for genetic characterization of circulating influenza viruses which would be crucial for preparation against pandemic and epidemic outbreaks in the future.