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Degradation of Phenol in Water Using Circulation Dielectric Barrier Plasma Reactors (순환식 유전체 장벽 플라즈마 반응기를 이용한 수중 페놀 처리)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Health Sciences
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    • v.38 no.3
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    • pp.251-260
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    • 2012
  • Objectives: The purpose of this study was evaluating the applicability of the circulation dielectric barrier plasma process (DBD) for efficiently treating non-biodegradable wastewater, such as phenol. Methods: The DBD plasma reactor system in this study consisted of a plasma reactor (discharge, ground electrode and quartz dielectric tube, external tube), high voltage source, air supply and reservoir. Effects of the operating parameters on the degradation of phenol and $UV_{254}$ absorbance such as first voltage (60-180 V), oxygen supply rate (0.5-3 l/min), liquid circulation rate (1.5-7 l/min), pH (3.02-11.06) and initial phenol concentration (12.5-100 mg/l) were investigated. Results: Experimental results showed that optimum first voltage, oxygen supply rate, and liquid circulation rate on phenol degradation were 160 V, 1 l/min, and 4.5 l/min, respectively. The removal efficiency of phenol increased with the increase in the initial pH of the phenol solution. To obtain a removal efficiency of phenol and COD of phenol of over 97% (initial phenol concentration, 50.0 mg/l), 15 min and 180 minutes was needed, respectively. Conclusions: It was considered that the absorbance of $UV_{254}$ for phenol degradation can be used as an indirect indicator of change in non-biodegradable organic compounds. Mineralization of the phenol solution may take a relatively longer time than that required for phenol degradation.

Inhibitory effects of crude polysaccharide fractions from Annona muricata L. on melanogenesis (그라비올라 잎(Annona muricata L.) 조다당 분획분의 멜라닌 생성 저해 효과)

  • Kim, Yi-Eun;Byun, Eui-Hong
    • Korean Journal of Food Science and Technology
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    • v.51 no.1
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    • pp.52-57
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    • 2019
  • The objective of this study was to evaluate the anti-melanogenic effects of crude polysaccharide fractions from Annona muricata L. (ALP) in 3-isobutyl-1-methylxanthine (IBMX) stimulating hormone-induced mouse B16F10 melanoma cells. The inhibitory effect of ALP on tyrosinase activity was approximately $33.88{\pm}0.79%$ at 5 mg/mL. Additionally, the B16F10 cellular tyrosinase and melanin synthesis inhibition activities by ALP were $54.21{\pm}4.76$ and $56.74{\pm}6.97%$ at $250{\mu}g/mL$, respectively. Similarly, whitening-related protein tyrosinase, tyrosinase-related protein 1 (TRP-1) and TRP-2, and microphthalmia-associated transcription factor (MITF) were reduced by ALP treatment. These results indicated that ALP could be used as a functional cosmetic ingredient after confirming its whitening activity related to melanin content.

Removal Efficiency of Cryptosporidium Tracer in Drinking Water Treatment Process (정수처리 공정에서 Cryptosporidium Tracer의 제거효율)

  • Lee, Shun-Hwa;Kim, Yun-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.12
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    • pp.1304-1309
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    • 2006
  • In this study, removal efficiencies of treatment processes with C. tracer which is similar to the characteristics of Cryptosporidium were investigated. The highest removal efficiency of C. tracer was 97.16% when the input dose of PACI(Poly aluminium chloride, $Al_2O_3$(10%)) was 10 mg/L. The higher turbidity and SS removal efficiencies were, the more C. tracer cohesion efficiency increased. Also when pH of the raw water was high, removal efficiency of C. tracer increased. As the correlationship($R^2$) between effluent turbidity after coagulation-precipitation and removal efficiency of C. tracer was 0.9506, removal efficiency of Cryptosporidium could be evaluated by effluent turbidity after coagulation-precipitation. Also the range of C. tracer removal efficiency by sand filtration was $94.00{\sim}95.83%$ and the correlationship($R^2$) between effluent turbidity after filtration and removal efficiency of C. tracer was 0.8704. Therefore, when filtration-effluent turbidity is good under the optimized coagulation condition, removal efficiencies of Cryptosporidium by coagulation-precipitation, sand rapid filtration and sand rapid filtration after coagulation-precipitation are estimated as 1.55 log(97.16%), 1.38 log(95.83%) and 2.31 log(99.51%) respectively.

Development of Value-added Ketchup Products with Korean Chile Peppers(Capsicum annuum L.) and Their Sensory Evaluation (국내산 고추를 이용한 고추 케찹 개발 및 관능적 특성 평가)

  • Lee, Seul;Yoo, Kyung-Mi;Song, Soon-Ran;Park, Jae-Bok;Hwang, In-Kyeong
    • The Korean Journal of Food And Nutrition
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    • v.25 no.1
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    • pp.9-16
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    • 2012
  • The purposes of this study were to develop value-added sauce(ketchup) products with Korean advanced chile peppers (Capsicum annuum L.), determine their physicochemical characteristics, and conduct a sensory evaluation. American chile ketchup products were collected from American local favorites and analyzed based on their compositions. The Korean chile ketchup contained tomato paste(37.5%), Korean chile pepper(14.1%), sugar(14.8%), vinegar(14.1%), garlic(8.5%), herbs, plum extract, and oligosaccharide. Its physiochemical analysis showed: moisture $59.61{\pm}0.28%$, crude protein $2.18{\pm}0.11%$, crude lipid $1.99{\pm}0.04%$, crude ash $9.26{\pm}0.13%$, crude carbohydrate $26.97{\pm}0.48%$, reducing sugar $35.19{\pm}0.97%$, salt $3.04{\pm}0.04%$, acidity $2.22{\pm}0.01%$, pH $3.7{\pm}0.01$, and $^{\circ}brix\;36.3{\pm}0.14$. Korean chile ketchup showed higher overall acceptability compared to American local favorite chile ketchup. This result suggests the possibility for replacing chile ketchup products imported from foreign countries(USA and Europe).

Evaluation of Affecting Factors on N and P removal in Biological SND (Simultaneous Nitrification and Denitrification) Process with NADH Sensor (NADH 센서를 이용한 생물학적 동시 탈질.질산화공정에서 질소, 인제거 영향인자 및 거동 평가)

  • Kim, Han-Lae;Lee, Si-Jin
    • Journal of Environmental Health Sciences
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    • v.34 no.5
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    • pp.374-381
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    • 2008
  • In this study, the factors affecting biological N and P removal using SND (simultaneous nitrification and denitrification) process were investigated and evaluated to examine the possibility of treating N and P through SND with NADH by surveying N and P traces in an aeration tank. Variations of $NH_4^+$-N+$NO_3^-$-N concentration were used to estimate the degree of SND in each point (P2, P3, P4, P5) of the aeration tank and these variations showed that denitrification efficiency in P2 (front zone), nitrification and denitrification efficiencies in P4 (middle zone) were 67%, 86% and 39%, respectively. When $PO_4^{-3}$-P concentration was analyzed in each point of the aeration tank, it was shown that $PO_4^{-3}$-P concentration coming into P2 was 1.25 mg/L, which increased to 2.22 mg/L by P release in P2 zone and then decreased to 0.74 mg/L by P uptake in P4. Consequently, we were able to estimate which high P removal efficiency observed in this study was caused by biological phosphorus removal. To determine the operating factors affecting effluent T-N, we analyzed the correlation among FN/M ratio, C/N ratio, Temp., SRT etc and these results showed that the correlation among FN/M ratio, C/N ratio and Temp was not high. However, the relationship of SRT and other parameters (effluent $NH_4^+$-N and effluent BOD) and the short SRT could have an affect on effluent $NH_4^+$-N and so effluent BOD could be increased. Thus, SRT operation should be controlled over 10 days. The results for analyzing the correlation between SRT and influent $NO_3^-$-N in order to investigate the operating factors affecting effluent T-P showed that T-P or $PO_4^{-3}$-P was not highly correlation with SRT, whereas $PO_4^{-3}$-P concentration increased along with increasing $NO_3^-$-N concentration into P2. Based on these results, we concluded, using regression analysis (R2=0.97), that effluent $PO_4^{-3}$-P concentration depends on $NO_3^-$-N concentration into P2.

A Newly Identified Glutaminase-Free L-Asparaginase (L-ASPG86) from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens

  • Lee, Su-Jin;Lee, Youngdeuk;Park, Gun-Hoo;Umasuthan, Navaneethaiyer;Heo, Soo-Jin;Zoysa, Mahanama De;Jung, Won-Kyo;Lee, Dae-Won;Kim, Hanjun;Kang, Do-Hyung;Oh, Chulhong
    • Journal of Microbiology and Biotechnology
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    • v.26 no.6
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    • pp.1115-1123
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    • 2016
  • L-Asparaginase (E.C. 3.5.1.1) is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. We identified the L-asparaginase gene (L-ASPG86) in the genus Mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. Following phylogenetic analysis, the deduced amino acid sequence of L-ASPG86 (L-ASPG86) was grouped as a type I asparaginase with respective homologs in Escherichia coli and Yersinia pseudotuberculosis. The L-ASPG86 gene was cloned into the pET-16b vector to express the respective protein in E. coli BL21 (DE3) cells. Recombinant L-asparaginase (r-L-ASPG86) showed optimum conditions at 37-40℃, pH 9. Moreover, r-L-ASPG86 did not exhibit glutaminase activity. In the metal ions test, its enzymatic activity was highly improved upon addition of 5 mM manganese (3.97-fold) and magnesium (3.35-fold) compared with the untreated control. The specific activity of r-L-ASPG86 was 687.1 units/mg under optimum conditions (37℃, pH 9, and 5 mM MnSO4).

Analysis of Image Quality and Optimized Reconstruction Window through Heart Rate and Its Variation in Retrospectively ECG-gated Coronary Angiography Using Multi-Detector Row CT

  • Lee, Sang-Ho;Park, Byoung-Wook;Kim, Hee-Joung;Haijo Jung;Kang, Won-suk;Son, Hye-Kyung;Choe, Kyu-Ok
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.461-463
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    • 2002
  • Image quality and selection of optimized window for good quality reconstruction in coronary angiography using multi-detector row CT (MDCT) have not been studied by heart rate and its variation. Therefore, the effect of heart rate and its variation was systemically analyzed. Eighty-three patients were undergone contrast-enhanced coronary angiography using MDCT. In this study, sixty cases were enrolled. Two radiologists graded image quality as follows: 4, excellent; 3, good; 2, fair; l, bad. The starting points of the reconstruction window were chosen at seventy and forty percent of R wave interval. Optimized window was scored as 1 when 40% reconstruction was better quality than 70%, as 2 when 40% reconstruction is same as 70%, and as 3 when 70% reconstruction was better than 40%. Regression analysis was performed. The range of variation of beats per minute (BPM) was well correlated with image quality (r=-0.55, p=0.000), however correlation with optimized window percentage was not statistically significant (p=0.969). By contraries, median value of BPM was comparatively well correlated with optimized window grade (r=-0.24, p=0.086). Median value of BPM was not well correlated with image quality (r=0.l70, p=0.l97). Image quality is more affected by variation of heart rate (VHR) than by higher heart rate. Selection of optimized reconstruction window for good image quality is mainly affected by heart rate and there is a tendency that systolic phase reconstruction is better in image quality than diastolic reconstruction in higher heart rate.

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Growth Characteristics of Candida parapsilosis Isolated from Deteriorated Ginseng Extract (인삼추출물로부터 분리한 Candida parapsilosis의 생육특성)

  • 양재원;유태종;김영배
    • Microbiology and Biotechnology Letters
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    • v.13 no.4
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    • pp.371-376
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    • 1985
  • We investigated the growth parameters of Candida parapsilosis isolated from spoiled red ginseng extract. The optimum pH range for C. parapsilosis was 6.0, whereas the minimum and maximum pH values that permitted growth were 3.0 and 11.0, respectively. For cells grown in PG medium plus 0 and 60% sucrose, the optimum water activity(Aw) values were 0.98 and 0.97, respectively. The optimum temperature for C. parapsilosis were 3$0^{\circ}C$ at an Aw of 0.90 in 4.8% potato dextrose broth with 18% sucrose (PGS). Cations inhibiting the growth of C parapsilosis were L $i^{+}$ $Ca^{2+}$, $Mg^{+2}$, $K^{+}$ in decreasing order, while anions were S $O_4$$^{2-}$, N $O_3$$^{[-10]}$ , C $l^{[-10]}$ .TEX> .

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Feasibility Study of the Stabilization for the Arsenic Contaminated Farmland Soil by Using Amendments at Samkwang Abandoned Mine (삼광광산 주변 비소 오염 토양에 대한 안정화 공법 적용성 평가)

  • Lee, Jung-Rak;Kim, Jae-Jung;Cho, Jin-Dong;Hwang, Jin-Yeon;Lee, Min-Hee
    • Economic and Environmental Geology
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    • v.44 no.3
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    • pp.217-228
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    • 2011
  • The feasibility study for the stabilization process using 5 amendments was performed to quantify As-immobilization efficiency in farmland soils around Samkwang abandoned mine, Korea. For the batch experiments, with 2% and 3% of granular lime(2-5 mm in diameter), leaching concentration of As from the soil decreased by 86% and 95% respectively, compared to that without the amendment. When 5% and 10% of granular limestone was added in the soil, As concentration decreased by 82% and 95%, showing that lime and limestone has a great capability to immobilize As in the soil. From the results of batch experiments, continuous column(15 cm in dimeter and 100 cm in length) tests using granular lime and limestone as amendments was performed. Without the amendment, As concentration from the effluent of the column ranged from 167 ${\mu}g$/L to 845 ${\mu}g$/L, which were higher than Korea Drinking Water Limit(50 ${\mu}g$/L). However, only with 1% and 2% of lime, As concentration from the column dramatically decreased by 97% for 9 years rainfall and maintained below 50 ${\mu}g$/L. With 5% of limestone and the mixed amendment(1% of lime + 2% of limestone), more than 95% diminution of As leaching from the column occurred within I year rainfall and maintained below 20 ${\mu}g$/L, suggesting that the capability of limestone to immobilize As in the farmland soil was outstanding and similar to that of lime. Results of experiments suggested that As stabilization process using limestone could be more available to immobilize As from the soil than using lime because of low pH increase and thus less harmful side effect.

Biological treatment process for Food wastewater Using ER-1 bioreactor (다단 수직형 호기성반응기(ER-1)를 이용한 음식물 폐수의 생물학적 처리기술)

  • Lee, Jae-ki;Choi, Hong-Bok;Shin, Eung-Bai;Park, Ju-Hyoung;Choi, Eun-Ju;Kim, Jung-Rae;Park, Young-Sook
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.4
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    • pp.113-120
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    • 2006
  • Wastewater generated through the food waste recycling process have known high concentration, BOD 20,000~150,000 mg/L, which has to treat to the proper level because of a ban on reclamation. But it is impossible to treat less than 10 days by existing water treatment plant. Ecodays Ltd. is to treat this wastewater during 2~4 days by ER-1, which can simultaneously induce the modified PFR(Plug Flow Reactor) of the oxygen transfer rate, MLVSS concentration, and influent concentration to top from bottom of reactor. We tested the pilot test about low concentration food wastewater(BOD 16,500 mg/L) and high concentration food wastewater(64,431 mg/L) at the food waste recycling plant of H-Gun(20t/d). Hydraulic retention time(HRT) of ER-1 for low concentration food wastewater is 2.5day. In low concentration conditions, ER-1 treatment efficiency is to appear BOD 99%, COD 98%, TN 97%, and TP 96%. While ER-1 process for high concentration food wastewater treatment is composed 2 stages, which are to be HRT 2.5day for law wastewater and HRT 1.5 day for secondary treatment. In high concentration conditions, ER-1 treatment efficiency is to appear BOD 97%, COD 84%, TN 66%, and TP 95%. It is treated without temperature control about high temperature($50^{\circ}C$) to appear low treatment efficiency in high concentration conditions.

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