• Applied Chemistry for Engineering
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• v.8 no.1
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• pp.59-66
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• 1997

Di(10-hemisuccinyloxy)decyl muconate(DDM) and di(6-hemisuccinyloxy)hexyl muconate(DHM) were synthesized, and showed a phase transition in an aqueous solution at 97 and $79^{\circ}C$, respectively. They did not form liposomes by themselves or even in the presence of cholesterol or dioleoylphosphatidylethanolamine(DOPE), but did form liposomes when they were mixed with phosphatidylcholine(PC). DHM molecules in liposome membranes were readily polymerized via 1,2-polymerization process on exposure to 254nm. Liposomes composed of DOPE/dioleoylphosphatidylcholine(DOPC)/DHM(3/3/1) were stable at neutral pH, but leaky at weakly acidic pH. The leakage of entrapped calcein from liposomes in phosphate-buffered saline (PBS, $37^{\circ}C$) of pH 4.8 and 5.8 was complete within 30 and 50 min, respectively. The release of toke entrapped calcein was increased with decreasing pH such that 50% and 100% of the calcein were released at pH near 5.5 and 5.0, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.7
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• pp.946-951
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• 2010

The objective of this research was to evaluate the effects of dietary supplementation of blood meal (BM) as a source of histidine, and magnesium oxide (MgO) as a catalyst of carnosine synthetase, on carnosine (L-Car) content in the chicken breast muscle (CBM), laying performance, and egg quality of spent old hens. Four hundred eighty laying hens (Hy-Line$^{(R)}$ Brown), 95wk old, were allotted randomly into five replicates of six dietary treatments: T1; 100% basal diet, T2; 100% basal diet+MgO, T3; 97.5% basal diet+2.5% BM, T4; 97.5% basal diet+2.5% BM+MgO, T5; 95% basal diet+5% BM, T6; 95% basal diet+5% BM+MgO. Magnesium oxide was added at 0.3% of diets. The layers were fed experimental diets for 5wk. There were no significant differences in the weekly L-Car content in CBM among all treatments during the total experimental period, but some of the contrast comparisions showed higher L-Car in CBM of T6. The L-Car contents linearly decreased (p<0.01 or p<0.05) as the layers got older except in T4 (p>0.05). There were significant differences in egg weight (p<0.01) and soft and broken egg ratio (p<0.05). The control (T1) was highest in egg weight and T6 was lowest in soft and broken egg ratio. Among the parameters of egg quality, there were significant differences in eggshell strength (p<0.01) and egg yolk color (p<0.05). Magnesium oxide supplementation increased the eggshell strength and BM tended to decrease egg yolk color. Eggshell color, eggshell thickness, and Haugh unit were not influenced by BM and MgO. In conclusion, BM and MgO did not significantly influence the L-Car in CBM of spent layers. The L-Car content rapidly decreased as the layers became senescent. Eggshell strength was increased by MgO supplementation.

• Korean Journal of Food Science and Technology
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• v.34 no.2
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• pp.164-169
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• 2002

Five kinds of red wine from three different grape varieties, such as Gerbong (G), Campbell (C), Moru (M), Gerbong+Moru (70:30, GM), and Gerbong+Campbell(70:30, GC) were prepared in the year of 2000. The total phenolic content and color changes were monitored using hunter colorimeter and spectrophotometry methods during wine processing. The total phenolic content of Gerbong (G), Campbell (C), Moru (M), Gerbong+Moru(70:30, GM), and Gerbong+Campbell(70:30, GC) were 712.6 mg/L, 3472.9 mg/L, 2209.4 mg/L, 2019.4 mg/L, and 1184.5 mg/L, respectively. Between total phenolic content and hunter colorimeter value (L, a, and b) of red wine showed no significant difference, but that of spectrophotometry color value (hue and intensity) were significantly different (p<0.001). The total phenolic content, hue, and intensity values represented significantly different between grape varieties (p<0.01, p<0.001). the hue showed a negative correlation relationship $(r^2=0.8660,\;p<0.0001)$, and that of intensity was a positive $(r^2=0.8304,\;p<0.0001)$ between total phenolic contents. The total phenolic content of red wine could be estimated simply using these equations, $Y_{Total\;phenolic\;content\;(mg/L)}=3319.3-X_{Hue}/2208.36,\;and\;Y_{Total\;phenolic\;content\;(mg/L)}=1230.97-X_{intensity}/98.93$

• Journal of Korean Society of Environmental Engineers
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• v.28 no.3
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• pp.286-291
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• 2006

We studied possibility of mixing treatment of livestock wastewater and sewage using Phanerochaete chrysosporium PSBL-1. Our study showed that 97.6% of SS and 95% of T-P removal efficiency was achieved when 2 mL BF02(a coagulant) and 100 mL C-210EL(a cationic polymer) were added to the mixture(2:1, v/v) of livestock wastewater and sewage. We studied treatment characteristic of Phanerochaete chrysosporium PSBL-1, after were mixed pretreated wastewater and sewage by dillution ten times about livestock wastewater. The removal efficiency of NBDCOD(non-biodegradable COD), $NH_3-N$ and T-N was increased according to increase of pH. That is, T-N concentration of effluent was satisfied 60 mg/L by drain water waterqulity standard of livestock wastewater public treatment facilities with 35 mg/L from a lapse of five days at pH 6.7, 51 mg/L from a lapse of three days at pH 8 and 33 mg/L from a lapse of one day at pH 10. Moreover $COD_{Mn}$ concentration of effluent was satisfied 40 mg/L by drain water waterqulity standard of livestock wastewater public treatment facilities after a laps of one day at all pH. Organics and nitrogen concentrations of effluent were higher case with addition of V.A.(veratryl alcohol) than case without addition of V.A.(veratryl alcohol). $COD_{Mn}$ concentration of effluent satisfied drain water qulity standard of livestock wastewater public treatment facilities from a lapse of one day, when C/N rate(3:1) of influent was not controled. T-N satisfied that from a lapse of two days, when C/N rate was controled with $4{\sim}6$.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.1
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• pp.42-48
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• 2012

In this study, experiment on phosphorus removal was performed by using microbubble liquid film flotation tank with microbubble module. After dissolving gas and liquid in dissolving tank, microbubble liquid film system created microbubbles in equal size under fixed low pressure. After being passed through $A_2O$ and m-$O_3$ process, secondary treatment wastewater was used as influent in phosphorus removal process. When the T-P concentration of influent was 2.89 mg/L, alum(8%, 30 mg/L) was injected into a microbubble flotation tank, the treatment resulted 94% of T-P removal rate. Remaining T-P concentration was less than 0.2 mg/L, which is in accord with the effluent quality standard. Seasonal variations in water temperature showed no differences in T-P removal property. When the inflow concentration of SS was 1.0 mg/L or more, it served as coagulation nuclei in the coagulation process. In that condition, average T-P removal rate was higher than 97%. When 50% of floated scum was returned, coagulator Al included in scum assisted the injected coagulator and maximized the coagulation efficiency of pollutant. In such treatment, the T-P concentration was measured as 0.18 mg/L and satisfied the outflow water quality standard, which is 0.2 mg/L or less.

• Korean journal of applied entomology
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• v.51 no.2
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• pp.149-152
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• 2012

The larvicidal activities of Piper nigrum fruit methanol extracts and its constituents against larvae of the diamondback moth, Plutella xylostella, were investigated using the leaf dipping method. Administering the P. nigrum methanol extract resulted in 100 and 97% mortality against diamondback moth larvae at 5.0 and 2.5 mg/mL concentrations, respectively. Larvicidal activities of the P. nigrum fruit-derived piperidine alkaloids, piperine, and N-isobutylamide, as well as pellitorine, guineensine, pipercide, and retrofractamide A against P. xylostella varied according to test compound. Based on the 48 h $LC_{50}$ values, the most toxic compound to diamondback moth larvae was guineensine (0.013 mg/mL) followed by retrofractamide A (0.020mg/mL), pipercide (0.033mg/mL), and pellitorine (0.046 mg/mL). The $LC_{50}$ value of piperine was >0.5 mg/mL.

• KSBB Journal
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• v.15 no.3
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• pp.219-225
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• 2000

Both the production of high spore concentration and high insecticidal activity are required in the production of Bacillus thuringiensis to be used for the bacterial insecticide. In the production of high cell and spore concentrations of B. thuringiensis the continuous fed-batch culture(CFBC) and intermittent fed-batch culture(IFBC) were investigated at $28^{\circ}C$ by maintaining 40% dissolved oxygen concentration. When the final glucose concentration was 50 g/L the maximum viable cell number obtained using the CFBC with linear gradient feeding was $9.37{\times}109$ cells/mL and maximum spore concentration was $8.33{\times}109$ spores/mL which was approximately 84.4% yield of spore formation. When the final glucose concentration was 100 g/L the aximum viable cell and spore concentrations obtained using the IFBC with pH-statb were $1.38{\times}$1010 cells/mL and $1.35{\times}1010$ spores/mL respectively and the yield of spore formation was approximately 97.8%.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.3
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• pp.179-183
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• 2001

Shortnecked clam, Ruditapes philippinarum, is one of the very important shellfish produced in south-western coast of Korea. But it's ready to be polluted and have sand in flesh because it mainly inhabit in silt at the inside of coastal area. This study was carried out to obtain informations about the elimination of sand in shortnecked clam harvested from western coast of Korea. During rearing shortnecked clams in water tank, the elimination rates of sand from them at 6, 13, 23, $28^{\circ}C$ were 59.0, 88.2, 97.9, $96.1^{\circ}C$ after 48 hours, respectively. The sand was eliminated above $95^{\circ}C$ from the shellfish at 32,9 to $40\%_{\circ}$ of salinity after 48 hours. But the sand elimination from them was incomplete and inconsistent at 10 to 20 of salinity. The sand was eliminated rapidly at pH 1.9 to 9.0 however, its elimination was not effective at neutral or acidity range, It was found that the sand elimination was most effective at $23^{\circ}C, 35\%_{\circ}$ salinty and pH 9.0. Reasonable flow rate for sand eliminate in shortnecked clam was above 150 L/min./shellfish $m^3$ under both 3,000 and 4,000 L/shellfish $m^3$, and above 100 L/min,/shellfish $m^3$ under both 6,000 and 8,000 L/shellfish $m^3$ in water tank.

• Food Science and Preservation
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• v.15 no.6
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• pp.832-839
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• 2008

The stock that is the first step for preparation of soups and purees links to the taste of food. Many types of vegetable have been used in stocks, but this study focused on stocks prepared with sweet pumpkin. The stock preparation conditions including the weight of sweet pumpkin, the water volume, and the boiling time at $97^{\circ}C$ were optimized by response surface methodology. The quality characteristics of the resulting stock were investigated. The color, flavor, taste and overall acceptability were dependent parameters. A model equation was proposed with regard to the sweet pumpkin weight, water volume, and boiling time at $97^{\circ}C$. A sweet pumpkin weight of 357.9 to 403.0 g, a water volume of 689.8 to 768.5 mL, and a boiling time of 9.9 to 10.3 min at $97^{\circ}C$ were found to be the optimal stock preparation conditions. The quality characteristics of the sweet pumpkin stock prepared under the optimized conditions were pH 6.64, total acidity 0.18%, soluble solids $2.39\;^{\circ}Brix$, color value (L, 99.07 a, -2.43 b, 11.82), total polyphenol 280.75 mg/L, and electron donating ability 21.32%.

• Journal of Dairy Science and Biotechnology
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• v.30 no.1
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• pp.45-53
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• 2012

Lactobacillus acidophilus isolated from the feces of a 7-day-old breast-fed infant was characterized to examine the scope of its commercial use. Forty-three Lactobacillus strains, which could grow at pH 5.5, were isolated. From these Lactobacillus isolates, 14 Lactobacillus strains were selected, which demonstrated more than 80% viability and homofermentative lactic fermentation. Finally, 9 L. acidophilus strains (NB 201~NB 209) were identified as candidate strains based upon biochemical properties, carbohydrate utilization, and cellular fatty acid composition. L. acidophilus isolates demonstrated a survival rate of more than 80% when exposed to pH 2.5 for 2 h. In particular, L. acidophilus NB 204 showed a strong acid tolerance, with a 71% survival rate even at pH 2.0. L. acidophilus isolates also manifested high bile acid tolerance; more than 87% of the cells survived on agar containing 1% bile extract, except for L. acidophilus NB 206, which showed a 73% survival rate. All L. acidophilus isolates were confirmed to have proteolytic activity; L. acidophilus NB 204 and NB 209 yielded higher levels of TCA-soluble peptides and free amino acids. The ${\beta}$-galactosidase activity of the L. acidophilus isolates was in the range of 1.97~2.45 units/mL.