• Title/Summary/Keyword: P3b

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Study on the Antagonism of Useful Microbes against Soybean Sprout Rotting Pathogens and Their Effect on the Growth of Soybean Sprouts (갈항미생물이 콩나물 부패균에 미치는 향균능력과 콩나물 생육 특성)

  • 김도완
    • Culinary science and hospitality research
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    • v.9 no.4
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    • pp.113-122
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    • 2003
  • This study was conducted to examine the antagonism of useful microbes against soybean sprout rotting pathogens and their effect on the growth of soybean sprouts. The antagonism against soybean sprout rotting pathogens and the effect on the growth of soybean sprouts were examined by using P. areofacience 14H-3, P. fluorescens R1-12 and B. cereus Yell, bacteria were shown to inhibit mycellial growth of Rhizotonia solani strongly. The results of this study are summarized as follows. P. areofacience 14H-3 and B. cereus Yell were highly antagonistic against Rizoctonia solani, while they were especially highly antagonistic against bacterial diseases. The effect of inhibiting the proliferation of soybean sprout rotting pathogens was also examined by adding the culture solution for antagonistic bacteria to the PDA. Both P. areofacience 14H-3 and P. fluorescens Rl-12 showed the inhibition rate of 78.8%, while B. cereus Yell did 52.9%. The fresh weight and length of soybean sprouts were measured after raising them with added antagonistic microbes and culture medium. Soybean sprouts treated with B. cereus Yell showed increased higher, compared with those not treated with it. Soybean sprouts were also raised in the culture solution with antagonistic bacteria to examine the growth of soybean sprouts. Soybean sprouts treated with the culture solution of 200 times showed better growth than those not treated with it. Analyze proximate composition in soybean sprout showed that moisture, ash, total sugar did not appear difference, but in case of crude protein B. cereus Yell(8.9%) increased about 2 times than control(3.6%), but occasion of crude fat and crude fiber were P. areofacience 14H-3, P. fluorescens Rl-12 increased about each 2 times than control. In occasion of vitamin, bacterial antagonist(9.4∼10.8mg%) was more higher than control(9.9mg%).

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Factors Affecting Opinions on Marriage of Unmarried Women in Their 30's in Korea: Based on 2012 Social Survey (한국 30대 미혼여성의 결혼견해 영향요인: 2012 사회조사 기반)

  • Lee, Sung-Hee;Gweon, Hyun-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.1
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    • pp.606-616
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    • 2015
  • This study was conducted in order to identify factors affecting opinions on marriage of unmarried women in their 30's in Korea. Analysis of data on 529 unmarried women in their 30's, which were taken from the 2012 social survey, was analyzed. Data were analyzed using ${\chi}^2$ test, hierarchical logistic regression analysis as well as descriptive statistics by SPSS version 20.0. Results showed that age(Exp(b)=.90,p=.025), opinions on divorce(Exp(b)=3.81,p<.001) and remarriage(Exp(b)=3.09,p=.001), and value on marriage(Exp(b)=.80,p<.001) were independent predictors of opinions on marriage. Economic activity(Exp(b)=19.58,p=.003) was found to be an independent predictor of opinions on marriage for the 35-39 year old women differ from 30-34 year old women predictors. Findings of this study indicate a need for developing family and marriage value-centered education and the low-fertility policy for the 35-39year old actively working women considering factors affecting opinions on marriage.

Antigenotoxicity of Quercetin and Its Glycosides Against Benzo(a)pyrene-induced Genotoxicity (퀘르세틴 및 퀘르세틴 배당체들의 벤조피렌에 대한 유전독성억제효과)

  • Kim, Jeong-Han;Heo, Moon-Young
    • YAKHAK HOEJI
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    • v.42 no.4
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    • pp.414-421
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    • 1998
  • In order to compare the suppressive effect of quercetin and its glycosides, such as quercitrin (quercetin-3-rhamnoside), isoquercitrin (quercetin-3-glucoside), hyperin (querceti n-3-galactoside)and rutin (quercetin-3-rhamnosyl glucoside), on the genotocicity by benzo(a)pyrene(B(a)P), in vitro sister chromatid exchange(SCE) test using mouse spleen lymphocytes and in vivo micronucleus test using mouse peripheral blood were performed. B(a)P-induced SCEs in vitro were slightly decreased by the simultaneous treatment of quercetin and its glycosides, although there was no significant decrease. On the other hand, MNU induced micronucleated reticulocytes(MNRL7s) in vivo were significantly decreased with a dose-dependent manner in all compounds tested. However, there were no differences between quercetin aglycone and glycosides in the suppressive effects under experimental condition of this study. To elucidate, the action mechanism of quercetin aglycone and its glycosides against B(a)P-induced genotoxicity, the assay of DNA binding with B(a)P was studied. Quercetin aglycone and its glycosides inhibited B(a)P metabolism in the presence of S-9 mix and decreased the B(a)P/DNA binding in the calf thymus DNA with S-9 mix. These results suggest that antigenotoxicity of quercetin antiglycosides on B(a)P-induced genotoxicity is due to decrease of DNA binding with B(a)P through the inhibition of metabolism with B(a)P in the calf thymus DNA. Therefore, quercetin and its glycosides may act as an antigenotoxicity agent and may be useful as a chemopreventive agent of polycyclic aromaic hydrocarbons like B(a)P.

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UNDERWATER DISTRIBUTION OF VESSEL NOISE (선박소음의 수중분포에 관한 연구)

  • PARK Jung Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.10 no.4
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    • pp.227-235
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    • 1977
  • The noise pressure scattered underwater on account of the engine revolution of a pole and liner, Kwan-Ak-San(G. T. 234.96), was measured at the locations of Lat. $34^{\circ}47'N$, Long. $128^{\circ}53'E$ on the 16th of August 1976 and Lat. $34^{\circ}27'N$, Long. $128^{\circ}23'E$ on the 28th of July, 1977. The noise pressure passed through each observation point (Nos. 1 to 5), which was established at every 10m distance at circumference of outside hull was recorded when the vessel was cruising and drifted. In case of drifting, the revolution of engine was fixed at 600 r. p. m. and the noise was recorded at every 10 m distance apart from observation point No. 3 in both horizontal and vertical directions with $90^{\circ}$ toward the stern-bow line. In case of cruising, the engine was kept in a full speed at 700 r.p.m. and the sounds passed through underwater in 1 m depth were also recorded while the vessel moved back and forth. The noise pressure was analyzed with sound level meter (Bruel & Kjar 2205, measuring range 37-140 dB) at the anechoic chamber in the Institute of Marine Science, National Fisheries University of Busan. The frequency and sound waves of the noise were analyzed in the Laboratory of Navigation Instrument. From the results, the noise pressure was closely related to the engine revolution shelving that the noise pressure marked 100 dB when .400 r. p. m. and increase of 100 r. p. m. resulted in 1 dB increase in noise pressure and the maximum appeared at 600 r. p. m. (Fig.5). When the engine revolution was fixed at 700 r. p. m., the noise pressures passed through each observation point (Nos. 1 to 5) placed at circumference of out side hull were 75,78,76,74 and 68 dB, the highest at No.2, in case of keeping under way while 75,76,77,70 and 67 dB, the highest at No.3 in case of drifting respectively (Fig.5). When the vessel plyed 1,400 m distance at 700 r.p.m., the noise pressure were 67 dB at the point 0 m, 64 dB at 600m and 56 dB at 1,400m on forward while 72 at 0 m, 66 at 600 m and 57 dB at 1,400 m on backward respectively indicating the Doppler effects 5 dB at 0 m and 3 dB at 200 m(Fig.6). The noise pressures passed through the points apart 1,10,20,30,40 and 50 m depth underwater from the observation point No.7 (horizontal distance 20 m from the point No.3) were 68,75,62,59,55 and 51 dB respectively as the vessel was being drifted maintaining the engine revolution at 600 r. p. m. (Fig. 8-B) whereas the noise pressures at the observation points Nos.6,7,8,9 and 10 of 10 m depth underwater were 64,75,55,58,58 and 52 dB respectively(Fig.8-A).

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Clinical Features of Severely Constipated Children: Comparison of Infrequent Bowel Movement and Fecal Soiling Groups

  • Lee, Gyung;Son, Jae Sung;Bae, Sun Hwan
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.23 no.1
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    • pp.26-34
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    • 2020
  • Purpose: To compare the clinical features, diagnostic findings, and medications of children with infrequent bowel movements or fecal soiling. Methods: This study enrolled 333 children (189 male; age range, 1 month to 18 years) diagnosed with functional constipation by Rome III or IV criteria. We classified them into 3 groups (infrequent bowel movement without fecal soiling [G3-a], infrequent bowel movement with fecal soiling [G3-b], and fecal soiling only [G3-c]) and into 2 subgroups of fecal soiling (G2-b) or not (G2-a). Retrospective data on clinical characteristics, colon transit time (CTT) test results, and medications were collected. The Wilcoxon rank-sum test, Kruskal-Wallis test, Chi-square test, and Fisher's exact test were used for the statistical analysis. Results: The median age (months) and interquartile range (IQR) was 33 (45) in G3-a, 54 (40) in G3-b, and 73 (48) in G3-c (p<0.0001). G3-c had the latest onset (median, 18; IQR, 18; p=0.0219) and longest symptom duration (24 [24], p=0.0148). PEG 4000 was used in 60.6% (G3-a), 96.8% (G3-b), and 83.2% (G3-c) of patients (p<0.0001). The median age (months) and IQR were 33.0 (45.0) in G2-a and 63.5 (52.5) in G2-b (p<0.0001). G2-b had later onset (median, 12; IQR, 19.5; p=0.0062) and longer symptom duration than G2-a (24 [12], p=0.0070). PEG 4000 was used in 60.6% (G2-a) and 88.3% (G2-b) of children (p<0.0001). No statistically significant intergroup differences were seen in maintenance laxative dose, CTT, or CTT type. Conclusion: Infrequent bowel movement and fecal soiling represent the advanced stage of chronic functional constipation.

Purification of Alginate Lyase from Streptomyces violaceoruber and the Growth Activity of Intestinal Bacteria by Degree of Polymerization of Alginate Hydrolysates (Streptomyces violaceoruber 유래 Alginate Lyase의 정제 및 Sodium Alginate 가수분해 올리고당의 중합도별 Bifidobacterium spp.과 Lactobacillus spp.에 대한 생육활성)

  • Yoon, Min;Park, Young-Seo;Park, Gwi-Gun
    • Food Engineering Progress
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    • v.21 no.2
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    • pp.103-109
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    • 2017
  • Alginate lyase from Streptomyces violaceoruber was purified by DEAE sephacel chromatography and SP sepharose chromatography. The specific activity of the purified enzyme was 14.6 units/mg protein, representing a 40.6-fold purification of the crude extract. The final preparation thus obtained showed a single band on Tricine-SDS polyacrylamide gel electrophoresis whose molecular weight was determined to be 23.3 kDa. The polyMG block of sodium alginate was hydrolyzed by the purified alginate lyase and then separated by activated carbon column chromatography and bio gel P-2 gel filtration. The main hydrolysates were composed of hetero type M/G-oligosaccharides with the degrees of polymerization (D.P.) being 6 and 8. To investigate the effects of hetero type M/G-oligosaccharides from the sodium alginate on the growth of some intestinal bacteria, cells were cultivated individually on the modified-MRS medium containing D.P. 6 and 8 M/G-oligosaccharides. B. longumgrew 4.25-fold and 6.44-fold more effectively by the treatment of D.P. 6 and 8 M/G-oligosaccharides compared with those of standard MRS medium. In addition, B. bifidumgrew 3.3-fold and 5.4-fold more effectively by the treatment of D.P. 6 and 8 M/G-oligosaccharides. In conclusion, D.P. 8 was more effective than D.P. 6 hetero M/G-oligosaccharides as regards the growth of Bifidobacteriumspp. and Lactobacillus spp.

Paleoecological Aspect using Palynology since 4,000 Year B. P. in the Lowland of Western Central Korea (화분분석에 의한 한국 중서부 저지대의 4,000년전 이후 고환경)

  • 이상헌;전희영;윤혜수
    • The Korean Journal of Quaternary Research
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    • v.13 no.1
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    • pp.1-23
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    • 1999
  • A palynological investigation of the Annyeong-ri, Hwasung-gun Gyeonggi-do, in where Yung-Nung and Gun-Nung are located, was carried out for the first time. From the pollen analysis, two pollen zones are established: Pollen Zone I : Ainus-Quercus-Pinus and Pollen Zone Ⅱ : Pinus. Pollen Zone I is characterized by Mixed coniferous and deciduous broadleaved vegetation during about 3,500 yr. B.P.-2,000 yr. B.P., while after 2,000 yr. B.P. Pollen Zone Ⅱ appears to be Coniferous vegetation and Evergreen broadleaved vegetation. The former reflects there was a lowland fluvio-lacustrine environment before fully lacustrine one which represents the latter. Pollen analysis indicates the vegetation before 2,000 yr. B.P. was controled by environmental factor such as climate conditions, whereas after that time vegetation was strongly effected by human's impact. It is concluded that pollen analysis is useful to find out the relationship between vegetation history and man's interference. In addition freshwater algae play to tell the lake environments.

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Quality and Antioxidant Activity of Wet Noodles Supplemented with Brown Rice and Sorghum Powders (현미 및 수수 첨가에 따른 생면의 품질특성과 항산화 효과)

  • Cheng, Li;Kim, Sin-Joung;Kil, Jeung-Ha;Park, Kun-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.530-536
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    • 2014
  • In this study, we investigated the quality and antioxidant activity of wet noodles fortified by adding brown rice and sorghum powders. Wet noodles were divided into four groups: WN-p (wheat flour 100%, purified salt 2%), WBN-b (wheat flour 80%, brown rice powder 20%, bamboo salt (${\times}1$) 2%), WBSN-b (wheat flour 80%, brown rice powder 10%, sorghum powder 10%, bamboo salt (${\times}1$) 2%), and WSN-b (wheat flour 80%, sorghum powder 20%, bamboo salt (${\times}1$) 2%). The wet noodles were evaluated for their quality characteristics and capacities to scavenge free radicals. The weight, volume, capacity to absorb water, and turbidity of cooked WBSN-b were close to those of cooked WN-p. Springiness, cohesiveness, and chewiness of cooked WBSN-b were the highest among all cooked noodles added with brown rice or sorghum powders and textural properties of cooked WBSN-b were not significantly different from WN-P. In the sensory evaluation, the overall acceptance of WBSN-b received the highest score of 6.4 points, which was higher than the score for WN-p. DPPH and hydroxyl radical scavenging activities increased significantly with addition of brown rice and sorghum powder, and radical scavenging activities of WBSN-b and WSN-b were the highest. In conclusion, wet noodles added with 10% brown rice powder, 10% sorghum powder, and 2% bamboo salt (${\times}1$) exhibited the same quality properties of WN-p. Addition of 10% brown rice powder, 10% sorghum powder, and 2% bamboo salt (${\times}1$) increased the sensory and antioxidant activities of wheat flour noodles.

Performance of Early Laying Period of Large-Type Korean Native Ducks (토종오리 대형종 산란초기 능력)

  • Kim, Hak-Kyu;Choo, Hyo-Jun;Kim, Chong-Dae;Heo, Kang-Nyeong;Lee, Myeong-Ji;Son, Bo-Ram;Kang, Bo-Seok;Suh, Ok-Suk;Choi, Hee-Cheol;Hong, Eui-Chul
    • Korean Journal of Poultry Science
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    • v.40 no.1
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    • pp.17-23
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    • 2013
  • This work was carried out to investigate the performance of early laying period of large-type Korean native ducks. One hundred sixty eight ducks were used in this work and divided into A and B strains (6 replications/strain, 14 birds/replication). The days at first egg of A and B strains were the age of 154.5 and 154.7 days, egg weights were 69.1 g and 65.5 g, and body weights were 3,187 g and 3,105 g at the period of laying the first egg, respectively. The days at sexual maturity of A and B strains were the age of 175.8 and 171.5 days, egg weights were 75.2 g and 74.9 g, and body weights were 3,245 g and 3,225 g at the period of laying the first egg, respectively. There were no significant differences on the day of first egg, the day of sexual maturity, egg weight and body weight at first egg and the sexual maturity, average body weight, and daily feed intake between A and B strains (P>0.05). The day of the sexual maturity of B strain was earlier than that of A strain (P<0.05). However, weekly body weight was the highest at the age of 28 weeks (P<0.05), and weekly feed intake increased after 26 weeks old (P<0.05). Egg production (%) of B strain was higher than that of A strain at the age of 22~24 weeks (P<0.05), but there was no significant difference on egg production (%) the other weeks. A number of egg laid of B strain was higher than A strain at 22~24 weeks old (P<0.05). Feed conversion ratio of A and B strains were 10.7 and 6.53 at 24~24 weeks old, respectively, so feed conversion ratio of A strain was higher than that of B strain (P<0.05). Weekly feed conversion ratio wes the highest at 20~22 weeks old, and significantly decreased until 24~24 weeks old, but were lowly maintained from the age of 24 weeks to the age of 30 weeks. These results provided the basic data on the record of early laying period of large-type Korean native ducks.

An Interferon Resistance Induced by the Interaction between HCV NS5B and Host p48 (C형 간염 바이러스 NS5B 단백질과 숙주의 p48 단백질의 상호작용에 의한 인터페론 저항성의 유도)

  • Park, So-Yeon;Lee, Jong-Ho;Myung, Hee-Joon
    • Microbiology and Biotechnology Letters
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    • v.36 no.4
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    • pp.353-359
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    • 2008
  • Hepatitis C virus (HCV) is known as the causative agent of blood transmitted hepatitis. Two viral proteins, E2 and NS5A, are known to exert interferon resistance of HCV via PKR pathway. Here, we report a third protein, the RNA-dependent RNA polymerase (NS5B) of HCV, induced interferon resistance inhibiting p56 pathway. p56 was shown to interact with p48 subunit of eukaryotic initiation factor 3 (eIF3). This interaction inhibited formation of ternary complex in translation initiation. Using dual reporter assay system, we observed that the translation decreased when interferon alpha was added to the culture. But, in the presence of HCV NS5B, the translation partly recovered. NS5B and p48 subunit of eIF3 were shown to interact. This interaction seems to inhibit the interaction between p48 and p56. This is the first report that a virus exerts interferon resistance via p56 pathway.