• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.3
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• pp.252-255
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• 1999

The quality characteristics of the Korean traditional salt-fermented fish sauces, the traditional anchovy sauce (TAS) and the sandlance sauce(TKS) were evaluated comparing to the commercial anchovy sauce (CAS) and sandlance sauce (CKS). The acidity was higher in TKS than in TAS, whereas the contents of VBN, total-N and amino-N were higher levels in TAS. In color values, L and b values in TAS were generally higher than those in TKS, whereas a and ${\Delta}E$ values were higher in TKS. The contents of total free amino acids in TAS and TKS were $12.40\;g\%$ (w/v) and $9.549 g\%$ (w/v), respectively. The contents or six amino acids, alanine, glutamic acid, leucine, isoleucine, valine and Iysine were higher in TAS, whereas the contents of arginine, glutamic acid, leucine, alanine and valine were higher in TKS. Nucleotides such as IMP and hypoxanthine were principal components in both TAS and TKS. The nitrogen related compounds, TMAO, TMA and total creatinine were determined to be $108.8\;mg\%$ (w/v), $60.5\;mg\%$ (w/v), $62.4\;mg\%$ (w/v) in TAS, and those in TKS were $60.1\;mg\%$ (w/v), $24.1\;mg\%$ (w/v), $67.6\;mg\%$ (w/v), respectively.

• Microbiology and Biotechnology Letters
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• v.41 no.4
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• pp.433-441
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• 2013

In this study, the anti-oxidative, anti-inflammatory, anti-melanogenic activities of Endlicheria anomala (Nees) Mez methanol extract (EAME) were evaluated by use of in vitro assays and cell culture model systems. The results revealed that EAME scavenges various radicals such as 1,1-diphenyl-2-picryl hydrazyl hydrogen peroxide induced reactive oxygen species, and lipopolysaccharide induced nitric oxide. Furthermore, EAME induced the expression of anti-oxidative enzymes such as heme oxygenase 1, thioredoxin reductase 1, NAD(P)H dehydrogenase 1, and their upstream transcription factor, nuclear factor-E2-related factor 2. Moreover, EAME inhibited in vitro DOPA oxidation and 3-isobutyl-1-methylxanthine induced melanogenesis in B16F10 cells. Its anti-melanogenic activity will have originated from the inhibition of tyrosinase enzyme activity and melanogenesis related protein expression. Taken together, these results provide the important new insight that E. anomala possesses various biological activities such as anti-oxidative, anti-inflammatory, and anti-melanogenic. Therefore, it might be utilized as a promising material in the fields of nutraceuticals and cosmetics.

• Environmental Analysis Health and Toxicology
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• v.23 no.3
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• pp.221-230
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• 2008

Chlorpyrifos (CPF) is a widely used organophosphate insecticide. Japanese medaka, Oryzias latipes was selected to investigate the effects of chlorpyrifos on survival rate, hatching rate, reproductivity and histopathological changes. Adult fish were exposed to CPF at concentrations of 20, 40, 80 and $160{\mu}g/L$, and the test duration was 4 weeks. An experiment with eggs was also performed under CPF exposure continued for 7 days covering from the fecundation to end-hatching stages. Survival rate of the adult medaka decreased in CPF $80{\mu}g/L$ and $160{\mu}g/L$ treatment groups compared with control, solvent control, $20{\mu}g/L$ or $40{\mu}g/L$L treatment group. Spontaneous start of feeding significantly decreased compared with the control (p < 0.01) when exposed to CPF at above 40 $\mu g/L$. Total spawning decreased by the 20 or $40{\mu}g/L$ CPF treatment compared with the control or solvent control in the test with eggs obtained from untreated adult medaka, and abnormal eggs increased in those CPF-treated groups. There were certain adverse effects at above $40{\mu}g/L$ determined from liver and gonad histological examinations. The results indicate that CPF has insignificant toxic effects in Japanese medaka at less than $20{\mu}g/L$ in long term exposure.

• Journal of Nutrition and Health
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• v.24 no.4
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• pp.299-307
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• 1991

The present study was designed to evaluate the effects of dietary coenzyme $Q_{10}$ on mitochondrial coenzyme $Q_{10}$ and fatty acid composition in adriamycin (ADR)-treated rats. Two experiments were conducted in rats. Experiment 1 was undertaken under the condition of simultaneous administration of ADR and coenzyme $Q_{10}$ for 4 weeks. Experiment 2 was undertaken under the same condition as experiment 1 after feeding the experimental diets alone without administration of ADR for 4 weeks. Heart mitochondrial coenzyme $Q_{10}$ level of rats was greatly decreased by ADR treatment. but higher level of dietary coenzyme $Q_{10}$ elevated this decrease to control ranges. Pretreatment with dietary supplementation of coenzyme $Q_{10}$ showed a significant increase in myocardial coenzyme $Q_{10}$ level. With ADR treatment. polyunsaturated fatty acids such as arachidonic acid (20 : 4) and docosahexaenoic acid (22 : 6) were decreased. However, dietary supplementation of coenzyme $Q_{10}$ modified this decrement to some extent. In both experiment 1 and 2. the polyunsaturated fatty acids/saturated and polyunsaturated fatty acids (P/S+ M) ratio of ADR-treated rats tended to be lower than that of control rats.

• YAKHAK HOEJI
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• v.57 no.3
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• pp.205-212
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• 2013

Dissolution test has been performed to control drug quality and to predict in vivo drug release profile of solid dosage forms, so there's a drift towards setting dissolution test instead of disintegration test. However, some solid dosage forms in Korea Pharmaceutical Codex (KPC) are not established the dissolution test yet, so these monographs are necessary to set the specification of dissolution test. In this study, we developed the specification and test method of dissolution test for itopride hydrochloride tablets and tiropramide hydrochloride tablets which are not established the dissolution test yet. According to the "Manual for Guideline Application for Validation of Analytical Procedures" and "Guidelines on Specification of Dissolution test for Oral dosage form" of Korean Pharmacopoeia (KP), we validated and established each development method. Based on the preliminary dissolution profile, we set the dissolution condition(paddle apparatus, pH 1.2 media, 50 rpm). For this condition, we performed the main dissolution test to determine the specification (45 min, 85%). Finally, we validated each analytical method by specificity, linearity, accuracy and precision. These developed methods will be included the next supplement of KPC and also contributed to the quality control of medicines.

• Journal of Korean Society on Water Environment
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• v.26 no.3
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• pp.377-386
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• 2010

Introduction of TBELs into Korean environmental regulatory system for wastewater may require very careful considerations and appropriate modifications of the TBELs applied in US. The Korean regulations for wastewater are based on uniform regulatory criteria for wastewater effluent discharge and are quite different from the individual permit system in US. In addition, the toxic pollutants regulated in Korea are much less than those in US. Therefore, the effects of TBELs application on the pollutants reduction and the economic feasibility should be carefully assessed for different categories of wastewater sources. In this study, the applicability of TBELs for the industrial categories of Korea was discussed. The TBELs were derived for a sample category, the pulp paper paperboard manufacture, based on the previously reported analytical data from 52 facilities of the domestic pulp paper paperboard manufacture in Korea. It was suggested that the BAT effluent limitations were BOD 30 mg/L, $COD_{Mn}$ 40 mg/L, SS 40 mg/L, T-N 30 mg/L and T-P 4 mg/L and that the allowable effluent loads were $0.31{\sim}1.75kgCOD_{Mn}/ton$-products. Due to the limitation of insufficient data, there were difficult to obtain the important factors to derive more systematic and accurate limitation standards for the pollutants such as the 'Long Term Average (LTA)', the 'Product Normalized Discharge Flow (PNDF)', and the 'Variability Factor (VF)'. However, as the first trial of TBELs determination based on the all available analytical data reported, the procedure and the outcome of the study may provide valuable insight on application of TBELs in Korea.

• Korean Journal of Weed Science
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• v.5 no.2
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• pp.137-142
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• 1985

This experiment was conducted to know the difference in weed population in the five cropping patterns kept same for six years from 1976 such as ricebarley, potato-rice, pea-rice, rice-rye, and rice-fallow. More and many weeds were growing in single cropping field than double cropping field. Dominant weed species in pea-rice and potato-rice cropping patterns were M. vaginalis and S. hotarui, M. vaginalis and P. distinctus. Coefficient of similarity of the cropping patterns between pea-rice and potato-rice appeared higher than single cropping system. However, pea-rice and rice-fallow cropping patterns showed low coefficient of similarity.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.5
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• pp.272-279
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• 2002

A field experiment was conducted to investigate the effect of N-use efficiency and rice yield by rapeseed cake(organic fertilizer) application at panicle initiation stage in machine transplating cultivation from 1999 to 2000. The rice yield increased by 4% in "50% rapeseed cake application", by 2% in "30% rapeseed cake application" at panicle initiation stage, respectively. Amount of fertilizer N uptake was high according to increasing amount of rapeseed cake application at panicle initiation stage compared with conventional treatment, but percentage recovery of fertilizer N was higher in 30% rapeseed cake application than in 50% rapeseed cake application at panicle initiation stage. Thus, this result was thought that there was more adventageous in 30% rapeseed cake application than 50% rapeseed cake application at panicle initiation stage in the fertilizer reduction or N-use efficiency respects in rice paddy.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.4
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• pp.424-431
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• 1996

This study was carried out to provide information for establishing counter measures of soil pollution through analysis of Cd. Cu, Pb, and Zn in paddy soils and brown rice. Cadmium, Cu, Pb, and Zn contents in soils were analyzed and distribution maps for these heavy metals were prepared. Heavy metal contents in brown rice were also measured. Average contents of Cd, Cu, Pb, and Zn in surface paddy soils extracted with 0.1 N HCl were 7.4, 35.8, 98.9, and $118.8mg\;kg^{-1}$, respectively. These were 9 times (Cu) to 50 times (Cd) higher than the background level of heavy metals in unpolluted paddy soils in Korea. The contents of Pb and Zn were lower than those measured in 1980, whereas Cd content did not decrease. The levels of heavy metal contamination in paddy soils may not affect growth or yield of rice plant, however, Cd contents indicated a level of serious concern to humans. The average contents of Cd, Cu, Pb, and Zn in brown rice were 0.38, 2.38, 1.31 and $22.31mg\;kg^{-1}$, respectively.

• Journal of Plant Biology
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• v.11 no.2
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• pp.1-30
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• 1968

The Han River was investigated monthly from April through September, 1967 at Chun-chon reservoir, Shin-yean, So-yang, Chung-pyong reservoir, Yang-soo-ri and Gi-doo-won to clarify the phytoplankton and to study its seasonal variation. At the same time, vertical distribution of phytoplankton was also studied from June through September at Chun-chon and Chung-pyong reservoir. The results obtained are as follows: As the environmental conditions, water temperature, dissolved oxygen, pH, and transparency were determined. The plankton samples collected from six stations were identified and classified by Engler's classification system. It resulted in 4 phyla, 4 classes, 3 subclasses, 13 orders, 27 families, 5 tribes, 71 genera, 222 species, 1 subspecies, 51 varieties and 2 forms. The total number of phytoplankton identified was 276. Of them, 84 species, 1 subspecies 26 varieties, 2 forms were recorded in this paper for the first time. Considering with the habitat of the total number of phytoplanktons, the highest percentage of Limnoplankton existed in the Chung-pyong reservoir. It suggests that hte Chung-pyong reservoir is becoming to have the character of lake. The planktons appeared commonly during six months at six station belong to Chrysophyta including 22 species in 11 genera. Among these 22 species, Navicula, consists of 6 species, was the most abundant. The number of species identified uniquely at each station were 5 species in the Chun-chon reservoir, 6 in Chung-pyong and Yang-soo-ri, 8 at Shin-yean, 11 at So-yang, and 18 at Gi-doo-won. The number of species occurred at all stations were 29 in spring, 42 in Autumn and during investigation period. The maximum production of total phytoplankton was generally shown in the late spring and the early fall with differences in quantity at six collecting statins. In the Chun-chon reservoir, the high concentration of approximately 1, 190, 000 cells per litter in the months of August and September was due to the increase of Chrysophyte genus, Melosira and the highest concentration of approximately 2, 000, 000 in July at Gi-doo-won was caused Chlorophyte genus, Secenedesmus. The Chun-chon reservoir of three years old was different from the Chung-pyong reservoir of 25 years old with respect to increase in Melosira genus in August and September. After the completion of the Ui-am reservoir, though there was an increase in total density, the number of species of phytoplankton was not changed. It suggests that Ui-am reservoir is similar to the Chun-chon reservoir under the influence of the latter. The density of total phytoplankton was generally high at the depth of 2m to 5m for the two reservoirs.