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Effect of Exposure Chlorpyrifos (CPF) on Survival, Hatching rate, Reproductivity and Histological Changes of Japanese Medaka, Oryzias latipes  

Kim, Byeong-Pyo (Department of Oceanography, Inha University)
Han, Kyung-Nam (Department of Oceanography, Inha University)
Publication Information
Environmental Analysis Health and Toxicology / v.23, no.3, 2008 , pp. 221-230 More about this Journal
Abstract
Chlorpyrifos (CPF) is a widely used organophosphate insecticide. Japanese medaka, Oryzias latipes was selected to investigate the effects of chlorpyrifos on survival rate, hatching rate, reproductivity and histopathological changes. Adult fish were exposed to CPF at concentrations of 20, 40, 80 and $160{\mu}g/L$, and the test duration was 4 weeks. An experiment with eggs was also performed under CPF exposure continued for 7 days covering from the fecundation to end-hatching stages. Survival rate of the adult medaka decreased in CPF $80{\mu}g/L$ and $160{\mu}g/L$ treatment groups compared with control, solvent control, $20{\mu}g/L$ or $40{\mu}g/L$L treatment group. Spontaneous start of feeding significantly decreased compared with the control (p < 0.01) when exposed to CPF at above 40 $\mu g/L$. Total spawning decreased by the 20 or $40{\mu}g/L$ CPF treatment compared with the control or solvent control in the test with eggs obtained from untreated adult medaka, and abnormal eggs increased in those CPF-treated groups. There were certain adverse effects at above $40{\mu}g/L$ determined from liver and gonad histological examinations. The results indicate that CPF has insignificant toxic effects in Japanese medaka at less than $20{\mu}g/L$ in long term exposure.
Keywords
chlorpyrifos; Oryzias latipes; toxicity; organophosphorous insecticide;
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