• Journal of Gynecologic Oncology
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• 제29권6호
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• pp.95.1-95.17
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• 2018

Objective: Cervical cancer is one of the most common malignant tumors. Our previous results showed that long non-coding RNA (lncRNA) XLOC_006390 plays an important role in cervical cancer. In this study, we have explored the mechanism of action of lncRNA XLOC_006390. Methods: LncRNA XLOC_006390 was proposed to exercise its function as a competing endogenous RNA (ceRNA), and its potential targeted miRNAs was predicted through the database LncBase Predicted v.2. Two miRNAs, miR-331-3p, and miR-338-3p, were chosen for the study. Expression of miRNAs and lncRNA in cervical cancer cells and tissues was detected by reverse transcription polymerase chain reaction. To determine the correlation, silencing of XLOC_006390, over-expression of miR-331-3p, and miR-338-3p was performed in SiHa and Caski cell lines, respectively. Results: Based on the interactive effect between miRNA and lncRNA, miR-331-3p and miR-338-3p were significantly downregulated in cervical cancer cells and tissues, and their expression levels were negatively related to that of lncRNA. Our results also showed that the expression of miR-331-3p target gene NRP2, miR-338-3p target genes PKM2, EYA2 was significantly downregulated when the XLOC_006390 was knocked down. Further, XLOC_006390 was found to facilitate cervical cancer tumorigenesis and metastasis by downregulating miR-331-3p and miR-338-3p expression. Conclusion: Taken together, our study demonstrated that XLOC_006390 may serve as a ceRNA and reversely regulates the expression of miR-331-3p and miR-338-3p, thus facilitating cervical cancer tumorigenesis and metastasis.

• Journal of Nutrition and Health
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• 제33권2호
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• pp.124-133
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• 2000

This study evaluated the effects of chronic ethanol consumption and/or taurine supplementation on hepatic total, phospholipid fatty acid composition and the metabolism of rats fed one of three purified liquid diets for 8 weeks. the rats followed either the control diet (CD, ethanol-free and taurine-free diet); ethanol diet (ED, CD+ 50g ethanol/L) or ethanol-taurine diet (ETD, ED+3.75g taurne/L). Chronic ethanol consumption and/or dietary taurine supplementation were associated with altered hepatic total and phospholipid fatty acid composition. compared to the values for the control rats, ED or ETD significantly decreased the percentage of total monounsaturated fatty acids ($\Sigma$MUFA), and increased the percentage of total polyunsaturated fatty acids ($\Sigma$PUFA) of hepatic total lipids(p〈0.01). Percentages of 14:0(P〈0.01) and 16:0(p〈0.001) were sigificantly lower, and those of 18:0(p〈0.01), 20:0(p〈0.001), 20:3$\omega$6(p〈0.01) and 22:4$\omega$6(p〈0.01) in hepatic total fatty acid compositions were oserved in rats fed ETD versus those fed ED or ETD. No significant differences in hepatic total fatty acid compositions were observed in rats fed ETD versus those fed ED. Percentages of 24:0(p〈0.01), 16:1(p〈0.05), 20:1(p〈0.01), 18:2$\omega$6(p〈0.01) and 18:3$\omega$3(p〈0.05) in hepati phospholipids were significantly higher, and those of 14:0(p〈0.01), 16:0(p〈0.001), 20:3$\omega$3(p〈0.05) in hepatic phospholipids were significantly higher, and those of 14:0(p〈0.01), 16:0(p〈0.001), 20:3$\omega$3(p〈0.001), 22:6$\omega$3(p〈0.001) and $\Sigma$$\omega$3(P〈0.001) were significantly lower in rats fed ED or ETD compared to the values for the control rats. The Δ5 desaturation index(20:3$\omega$6⇒20:4$\omega$6) and elongation index (20:5$\omega$3⇒22:5$\omega$3) of hepatic phospholipid index (20:3$\omega$6⇒20:4$\omega$6) and decreased Δ4 desaturation index (22:5$\omega$3⇒22:6$\omega$3) compared to the values for the ED rats. These changes in hepatic fatty acid composition induced by chronic ethanol consumption and/or taurine supplementation might be associated with the modulations of physical properties of the hepatic cell membrane and its sensitivity to peroxidation damage.

• Restorative Dentistry and Endodontics
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• 제37권1호
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• pp.16-23
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• 2012

Objectives: Present study was undertaken to investigate the crystal growth onto synthetic hydroxyapatite (HA) seeds in pH 4.3 and pH 7.0 supersaturated solutions with different fluoride concentrations. Materials and Methods: 8 groups of pH 4.3 and 7.0 calcium phosphate supersaturated solutions were prepared with different fluoride concentrations (0, 1, 2 and 4 ppm). Calcium phosphate precipitates yield crystal growth onto the HA seed surface while solutions flow. For evaluation of crystallizing process, the changes of $Ca^{2+}$, $PO{_4}^{3-}$, $F^-$ concentrations of the inlet and outlet solutions were determined. The recovered solid samples were weighed to assess the amount of minerals precipitated, and finally determined their composition to deduce characteristics of crystals. Results: During the seeded crystal growth, there were significantly more consumption of $Ca^{2+}$, $PO{_4}^{3-}$, $F^-$ in pH 4.3 solutions than pH 7.0 (p < 0.05). As fluoride concentration increased in pH 4.3 solution, $Ca^{2+}$, $PO{_4}^{3-}$, $F^-$ consumption in experimental solutions, weight increment of HA seed, and fluoride ratio in crystallized samples were increased. There were significant differences among the groups (p < 0.05). But in pH 7.0 solution, these phenomena were not significant. In pH 7.0 solutions, analyses of crystallized samples showed higher Ca/P ratio in higher fluoride concentration. There were significant differences among the groups (p < 0.05). But in pH 4.3 solution, there were not significant differences in Ca/P ratio. Conclusions: Crystal growth in pH 4.3 solutions was superior to that in pH 7.0 solutions. In pH 4.3 solutions, crystal growth increased with showed in higher fluoride concentration up to 4 ppm.

• 한국어류학회지
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• 제10권1호
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• pp.32-39
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• 1998

1996년 3월 인도네시아 아라푸라해에서 채집된 하스돔속(Pomadasys) 어류 3종을 대상으로 그들의 악골, 새파 및 인두골을 조사하였다. 전상악골 상행돌기를 두장에 대한 백분율로 환산하면, P. maculatus가 25.4~27.8%로 가장 크며, P. kaakan은 21.6~23.3%, P. argyreus는 15.3~21.0% 범위에 속한다. 전상악골 상행돌기를 전상악골에 대한 백분율로 환산하면, P. argyreus가 54.7~72.8%로 전상악골보다 상행돌기가 작으며, P. kaakan은 107.8~ 114.9%로 상행돌기가 전상악골보다 조금 크고, P. maculatus는 126.7~136.7%로 상행돌기가 전상악골보다 다소 크다. P. argyreus의 성장단계별 악골의 형태변화를 조사한 결과, 전상악골 상행돌기에서 증가경향을 보인다. 양턱에는 원뿔니가 띠를 형성하는데, P. argyreus가 가장 조잡한 형태를 보였고, P. kaakan은 다소 발달된 형태를, P. maculatus는 규칙적이고 강한 원뿔니가 조밀하게 나 있다. 인두치는 턱니보다 크고 굵은 원뿔니 및 바늘 모양의 날카로운 이빨을 가지는데, P. argyreus 는 P. kaakan과 P. maculatus보다 바늘 모양의 이빨이 더 많다. 새파는 P. maculatus 와 P. kaakan에서는 18~19개로 길이는 짧고 다소 굵은 반면에, P. argyreus는 22~24개로 길이가 길고 가늘다.

• 한국정보기술응용학회:학술대회논문집
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• 한국정보기술응용학회 2002년도 추계공동학술대회 정보환경 변화에 따른 신정보기술 패러다임
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• pp.331-335
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• 2002

인터넷의 발전은 사용자들의 정보에 대한 요구를 급속히 증가시키고 있으며, 고급 정보 획득을 위한 사용료에 대한 부담도 함께 증가하고 있다. 따라서 사용자 각자가 보유한 정보들을 다른 사용자들에게 오픈 시켜 공유할 수 있도록 하는 시스템의 개발에 대한 요구가 발생하게 되었다. 이러한 요구에 맞추어 본 논문에서는 네티즌들이 서로의 컴퓨터에 접속하여 상대방이 가진 정보들을 검색하고 공유할 수 있는 p2p(peer to peer) 시스템인 $\ulcorner$p2p-31$\lrcorner$을 제안하게 되었다. $\ulcorner$p2p-3$\lrcorner$ 시스템은 객체지향설계 기법인 UML을 이용하여 개발함으로써 시스템 구현의 효율성을 추구하였다. 또한 일반적인 p2P 시스템의 문제점으로 대두된 보안문제를 해결하기 위하여 3-tier 분산시스템을 이용하였다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2001년도 추계학술대회
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• pp.16-25
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• 2001

A genomic library of biphenyl-degrading strain Comamonas sp. SMN4 was constructed by using the cosmid vector pWE15 and introduced into Escherichia coli. Of 1,000 recombinant clones tested, two clones that expressed 2,3-dihydroxybiphenyl 1,2-dioxygenase activity were found (named pNB 1 and pNB2). From pNB1 clone, subclone pNA210, demonstrated 2,3-dihydroxybiphenyl 1,2-dioxygenase activity, is isolated. 2,3-Dihydroxybiphenyl 1,2-dioxygenase (23DBDO, BphC) is an extradiol-type dioxygenase that involved in third step of biphenyl degradation pathway. The nucleotide sequence of the Comamonas sp. SMN4 gene bphC, which encodes 23DBDO, was cloned into a plasmid pQE30. The His-tagged 23DBDO produced by a recombinant Escherichia coli, SG 13009 (pREP4)(pNPC), and purified with a Ni-nitrilotriacetic acid resin affinity column using the His-bind Qiagen system. The His-tagged 23DBDO construction was active. SDS-PAGE analysis of the purified active 23DBDO gave a single band of 32 kDa; this is in agreement with the size of the bphC coding region. The 23DBDO exhibited maximum activity at pH 9.0. The CD data for the pHs, showed that this enzyme had a typical a-helical folding structures at neutral pHs ranged from pH 4.5 to pH 9.0. This structure maintained up to pH 10.5. However, this high stable folding strucure was converted to unfolded structure in acidic region (pH 2.5) or in high pH (pH 12.0). The result of CD spectra observed with pH effects on 23DBDO activity, suggested that charge transition by pH change have affected change of conformational structure for 23DBDO catalytic reaction. The $K_m$ for 2,3-dihydroxybiphenyl, 3-metylcatechol, 4-methylcatechol and catechol was 11.7 $\mu$M, 24 $\mu$M, 50 mM and 625 $\mu$M.

• Journal of Animal Science and Technology
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• 제61권3호
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• pp.147-153
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• 2019

A goal for swine farming is the improvement in the number of live-born and weaned piglets per sow. Hence, the effect of parities should consider the correlation between the component traits of reproductive performance, weaning, and duration. Sows were housed in farrowing pens (W 2.2 ${\times}$ D 1.8 ${\times}$ H 1.2 m) on a partially slatted plastic floor. Twenty sows used in this study were between the first and sixth parity in gilts (P1), parity 2-5 (P3), and parity 6-9 (P6). Data collection by parity was classified into three categories: (1) reproduction performance (gestation length, total number of piglets born, number of piglets live born, number of piglets stillborn, total piglet birth weight; (2) weaning traits (weaning period, number of piglets weaning, total piglets weaning weight); (3) duration traits (farrowing duration, placenta expulsion duration, time from last piglet to first placenta, average birth interval, and tail wagging behavior). Gestation length was higher in P6 than P1 and P3 of different parity sows. The maximum value in P1 and P3 was 117 days, but the median value in P6 was 117 days (p < 0.05). The total number of piglets born (p = 0.113), number of piglets live born (p = 0.118), number of still piglets born (p = 0.151), and total piglet birth weight (p = 0.117) were not affected by parity. The number of live piglets was higher than the other parities by an average of $15.6{\pm}2.1$ in P1. The duration of farrowing was the lowest at 22.2 min in P6, but the maximum value was 42.2 min more than other parities (p = 0.355). Weaning traits of sows also did not differ significantly (p > 0.05), but the weaning period from P1 was lower than that of the other parities (p = 0.170). The number of piglets weaned was 10 heads on average in P1, P3, and P6 (p < 0.05). However, the mean values of the total piglet weaning weight (p = 0.377) of P6 (62.0 10.4 kg) were higher than those of P1 (54.9 10.2 kg) and P3 (58.4 13.6 kg). The placenta expulsion duration was higher in P6 than that in P1 and P3 (p = 0.447). The time from the last piglet to first placenta was be lower in P3 than that of the other parities (p = 0.206). The average birth interval was higher in P3 than that of the other parities (p = 0.156). Tail wagging (count) behavior was higher in P6 than in the other parities (p = 0.065). The data showed that the reproduction performance, total piglets born, and weight were higher in the gilts group, and higher in the weaning trait than that in the P6 group. This study examined the relationship between reproductive performance, birth interval and tail motion according to sow parity. Regarding duration, farrowing duration was lower in P6 than that in the other parities, but placenta expulsion and tail wagging were higher in P6 than in the other parities. Therefore, it is possible that the results from these sows could be used as basic data for effective farm management.

• 한국재료학회지
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• 제8권10호
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• pp.890-897
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• 1998

n-InP(001)기판과 PECVD법으로 ${Si}_3N_4$(200nm)막이 성장된 InP 기판사이의 direct wafer bonding을 분석하였다. 두 기판을 접촉시켰을 때 이들 사이의 결합력에 크게 영향을 주는 표면 상태를 접촉각 측정과 AFM을 통해서 분석하였다. InP 기판은 $50{\%}$ 불산용액으로 에칭하였을 때 접촉각이 $5^{\circ}$, RMS roughness는 $1.54{\AA}$이었다. ${Si}_3N_4$는 암모니아수 용액으로 에칭하였을 때 RMS roughness가 $3.11{\AA}$이었다. Inp 기판과 ${Si}_3N_4$/InP를 각각 $50{\%}$ 불산 용액과 암모니아수 용액에 에칭한 후 접촉시켰을 때 상당한 크기의 초기 겹합력을 관찰할 수 있었다. 기계적으로 결합된 시편을 $580^{\circ}C$-$680^{\circ}C$, 1시간동안 수소 분위기와 질소분우기에서 열처리하였다. SAT(Scanning Acoustic Tomography)측정으로 두 기판 사이의 결합여부를 확인하였다. shear force로 측정한 InP 기판과 ${Si}_3N_4$/InP사이의 결합력은 ${Si}_3N_4$/InP 계면의 결합력만큼 증가되었다. TEM과 AES를 이용해서 di-rect water bonding 계면과 PECVD계면을 분석하였다.

• 산업기술연구
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• 제20권A호
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• pp.45-50
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• 2000

A submerged cultivation of Ganoderma lucidum was carried out in an air-lift fermenter system, and the effects of different pH processes on extracellular branched ${\beta}$-1,3-glucan(EPS) production and mycelial growth(MDW) were investigated. The controlled pH process improved the production of branched ${\beta}$-1,3-glucan and biomass in comparison to the uncontrolled pH process. However, the maximum production of branched ${\beta}$-1,3-glucan were obtained by the bi-staged pH process. From these results, we confirmed that the bi-staged pH process was the most effective for improving the production of branched ${\beta}$-1,3-glucan from submerged culture of G. lucidum.

• Journal of Microbiology and Biotechnology
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• 제5권3호
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• pp.132-137
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• 1995

The lambda integrase (lnt) is believed to bind to several arm and core sites of attP DNA in order to facilitate intasome formation. We have done systematic mutagenic analysis on all 5 arm sites and found that P1 is absolutely required for integration while P2 is not. We also found that all 3 P' arm sites(P'1, P'2, and P'3) are required for efficient integrative recombination. P'1, which is an important binding site for excision, also seems to be crucial for integration when preincubation of attP DNA with Int and IHF is performed before recombination. Preincubation assay revealed that preincubation with Int and IHF improved the efficiency of recombination of wild type attP DNA and demolished recombinations of P'1 mutant attP DNAs.