• Title/Summary/Keyword: P242

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Identification and Fermentation Characteristics of Lactic Acid Bacteria that Produce Soy Curd With Low Sour Taste (저산미의 두유 커드를 생성하는 젖산균의 동정과 발효 특성)

  • Kim, Su-In;Jung, Min-Gi;Lee, Seung-Min;Kang, Moon-Sun;Seong, Jong-Hwan;Lee, Young-Geun;Kim, Han-Soo;Chung, Hun-Sik;Kim, Dong-Seob
    • Food Engineering Progress
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    • v.21 no.3
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    • pp.242-248
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    • 2017
  • The acidity of soy curd fermented by lactic acid bacteria is a major factor degrading the sensory properties of soy curd. For preparation of soy curd with low sour taste, lactic acid bacteria were separated from kimchi. The lactic acid bacteria which showed yellow-clear zone around the colonies on BCP plate and formed soy curd with low level of acidity were selected. The selected strain was analyzed by 16S rDNA sequence and named as Pediococcus inopinatus Y2. The maximum viable cell number of the soy curd fermented by P. inopinatus Y2 was obtained at 10.73 log CFU/mL at $25^{\circ}C$ for 24 h of fermentation. By the results of panel test, the overall sensory quality of the soy curd produced by P. inopinatus Y2 was higher than that of Leuconostoc mesenteroides No. 4395 and Lactobacillus sakei strain No. 383.

Factors affecting Person-Centered Care Competence for senior nursing students: Focused on King's dynamic interaction system model (고학년 간호대학생의 인간중심간호역량 영향요인: King의 역동적 상호작용 체계모형을 중심으로)

  • Cho, In Young;Yun, Ji Yeong;Kim, Hyojin
    • Journal of the Korea Convergence Society
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    • v.12 no.8
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    • pp.357-367
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    • 2021
  • We aimed to identify personal-interpersonal-social factors influencing Person-Centered Care Competence (PCCC) of senior grade nursing students based on King's dynamic interaction system model. The data were collected using online based questionnaires that contained items on ethical sensitivity, peer group care interaction, interpersonal competence, social support and PCCC. The participants were 242 4th grade nursing students who had undergone clinical practicum. The data were analyzed using descriptive statistics, T-test, ANOVA, Pearson's correlation coefficients, and Multiple regression analysis with the SPSS 26.0. In the multiple regression analysis, interpersonal competence(β=0.42, p<.001) and social support(β=0.29, p<.001) were statistically significant factors that explained 44.1% of PCCC(F=23.77, p<.001). This study is significant in that we identified the factors affecting the Person-centered Care competence based on King's conceptual framework. This study could provide basic data for the development of an intervention program to improve PCCC of senior nursing students.

Studies on the Ethanol Production by Clostridium thermosaccharolyticum (Clostridium thermosaccharolyticum에 의한 에탄올생산에 관한 연구)

  • 조은경;이윤광;변유량;유주현
    • Microbiology and Biotechnology Letters
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    • v.13 no.4
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    • pp.397-402
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    • 1985
  • The fermentation of various sugars by C. thermosaccharolyticum was examined under pH controlled, anaerobic condition. The kinetic model for Product formation at various sugars was the combination of growth and non-growth associated mode. In the utilization of a single sugar, glucose was the best carbon source for growth. The specific growth rate of glucose, xylose and cellobiose were 0.363 h$^{-1}$, 0.242 h$^{-1}$ and 0.144 h$^{-1}$ respectively. The production of ethanol from glucose showed a negatively growth associated mode, so the higher growth rate decreased the productivity of ethanol. The maximum concentrations of the produced ethanol were 2.42 g/l, 3.76 g/l, and 3.4 g/l on glucose, xylose, and cellobiose. No glucose was detected during cellobiose fermentation. Sequential utilization of sugars was observed in the mixtures of glucose, xylose and cellobiose. It preferred glucose, followed by xylose and then cellobiose. The presence of other sugars had little or no effect on the rate of another sugar utilization. Cell lysis at the end of fermentation occured more slowly in the mixtures of sugars than a single sugar.

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Development of Versatile Strains of Pseudomonas Degrading Various Persistent Aromatic Hydrocarbons (다양한 난분해성 방향족 탄화수소를 분해하는 Pseudomonas의 균주개발)

  • 이지현;최인성;박경량;박용근;이영록
    • Korean Journal of Microbiology
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    • v.28 no.3
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    • pp.236-242
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    • 1990
  • To develop the new strains of microorganisms having the degradative ability for various aromatic hydrocarbons, the hybrid plasmid pKG2 having the 2,4-Dichlorophenoxyacetic acid(2,4-D) degradative genes, the hybrid plasmid pKG3 containg the naphthalene degradative genes and TOL plasmid were introduced into Pseudomonas putida KUD 12 and P. putida KUP 10 by transformation or conjugation which originally have the degradative ability of the synthetic surfactants and phthalate esters, respectively. From P. putida KUD12, the new strains of P. putida KUD101(pKG2), KUD102(pKG3), KUD103(TOL), and KUD202(pKG3, TOL) were obtained, and KUD106(pKG2), KUD107(pKG3), KUD108(TOL) were originated from the P.putida KUP10. The degradative abilities in P. putida KUD101, KUD102 and KUD107 were similar with those of the original strains. The P. putida KUD103, KUD106 and KUD202 had a little lower and P. putida KUD108 had a better degradative abilitie than those of the original ones. In the case of mixed cultures, the mixed culture of KUD107 and KUD108 had a better degradative abilities than those of the other mixed cultures.

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Improving the Initial Effluent Water Quality of Rapid Sand Filter by Coagulants Injection (응집제주입에 의한 급속모래여과에서 초기유출수의 수질향상)

  • Kim Woo-Hang;Jeon Ji-Hoon
    • Journal of Environmental Science International
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    • v.15 no.3
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    • pp.237-242
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    • 2006
  • The purpose of this research was to investigate the efficiency of coagulants dose after backwashing. The turbidity of initial effluent was high after backwashing in the rapid sand filtration and the high turbidity was almost removed by coagulants dose into filter-sand after backwashing. It was found that the turbidity of initial effluent was well removed by all kinds of the coagulants used in this study. When filtration was performed input water with differentiated pH's, the turbidity of effluent was low at the range of pH 5 - pH 7. But the removal was not good about over pH 9. This result was considered into the existence forms of aluminium, $Al(OH)^{2+}\;and\;{Al(OH)_2}^+$ at pH 5. Cryptosporidiums of effluent were 4/ml for ten minutes immediately after back washing and 3/ml until sixty minutes. However, the case of coagulant dose after backwashing, Cryptosporidiums of effluent were 0.5/ml for ten minutes with no detection after twenty minutes.

Factors Affecting on Smartphone Addiction according to the Classification of Addiction-risk Groups among College Students: A Focus on Self-control (대학생의 스마트폰 중독 분류군 별 중독에 영향을 미치는 요인: 자기통제력을 중심으로)

  • Jang, In Sun;Park, Seungmi
    • Korean Journal of Adult Nursing
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    • v.27 no.6
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    • pp.634-643
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    • 2015
  • Purpose: The purpose of the study was to investigate the factors affecting on smartphone addiction according to the classification of addiction-risk groups among college students, focusing on self-control. Methods: A sample of 242 college students at K University in Seoul was recruited to participate in the study. A structured questionnaire composed of items for the self-rated smartphone addiction scale and self-control scale was used to collect data. Results: The number of students in smartphone addiction-risk group was 66 (27.3%). This study had 17.5% of the explanatory power, including perceived smartphone necessity (${\beta}=.330$, p=.007), and instant self-control (${\beta}=-.281$, p=.028) in addiction-risk group. Whereas, in non-risk group, the affecting factors included gender (${\beta}=.194$, p=.004), self-awareness of addiction (${\beta}=-.290$, p<.001), and instant self-control (${\beta}=-.281$, p<.001) with 31.3% of the explanatory power. Conclusion: There is a need to develop an intervention program to prevent the addiction of smartphones and to improve self-control among college students.

Development and Application of Timeout Protocol on OR (수술실 타임아웃 프로토콜 개발 및 적용)

  • Park, Jeong-Sook;Kim, Eun-Hee;Lee, Hye-Ran
    • Korean Journal of Adult Nursing
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    • v.20 no.2
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    • pp.353-363
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    • 2008
  • Purpose: This study was conducted to develop a timeout protocol as the process of patients verification in the operating room, and to evaluate its effects. Methods: The timeout protocol was developed based on the experience of practices and the universal protocol of JCAHO 2004. The subjects of this study were 192 surgical members working in the operating room at an university hospital in Daegu, Korea. Results: The timeout protocol was developed in six steps; participants verification, encouragement of compliance, verification of right 3 PSP, agreement of surgical team members, verification of the parties to a patient, patient agreement. The data which have been resulted from the experimental group show significantly higher than those of control group as follows; cognition(t = -6.580, p = .000), contents of performance; progress of anesthesiologist as leader(${\chi}^2$ = 29.029, p = .000), verification of right patient, right site and right procedure(${\chi}^2$ = 40.663, p = .000), participation of surgical team(${\chi}^2$ = 68.412, p = .000), and the number of cases of performance(${\chi}^2$ = 242.900, p = .000). Conclusion: It suggests that medical accidents caused by failures in a preoperative verification process can be prevented if a timeout is conducted active involvement and effective communication among surgical team members for a final verification of the correct patient, procedure, and site.

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Comparisons of food intake between breast cancer patients and controls in Korean women

  • Kim, Eun-Young;Hong, Yeong-Seon;Jeon, Hae-Myung;Sung, Mi-Kyung;Sung, Chung-Ja
    • Nutrition Research and Practice
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    • v.1 no.3
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    • pp.237-242
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    • 2007
  • The purpose of this study was to compare food intakes between Korean breast cancer patients and a healthy control group. We compared the intake of nutrients of 117 food items between Korean breast cancer patients (n=97) and age matched healthy controls (n=97). Nutrient intake was estimated using a quantitative food frequency questionnaire. The mean caloric intake of breast cancer patients and healthy controls was not significantly different. Breast cancer patients consumed significantly less fat and antioxidant nutrients such as vitamin A, retinol, ${\beta}-carotene$, vitamin C and vitamin E when compared to the control subjects. Among the food items, the intake of eggs (p<0.01), legumes (p<0.05), vegetables (p<0.05), seasonings (p<0.001), and oils and fats (p<0.01) in breast cancer patients was significantly lower than that in the controls. These results suggest that Korean breast cancer patients consumed less amount of soy and vegetables, which are rich source of antioxidant nutrients and phytosterols. Thus, dietary guidance to increase intake of these foods may be beneficial in the prevention of breast cancer.

MOLECULAR BREEDING OF GLUTATHIONE PRODUCING BACTERIAL STRAINS

  • Nam Yong-Suk;Lee Se Yong
    • Proceedings of the Microbiological Society of Korea Conference
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    • 1991.04a
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    • pp.237-242
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    • 1991
  • In order to increase the production of glutathione by maximizing the expression of recombinant gsh plasmids, two genes responsible for the biosynthesis of glutathione were cloned. A gshI gene was cloned onto pBR322 plasmid as 3.6Kb PstI DNA fragment from E. coli K-12 chromosomal DNA. Also gshII gene was cloned onto pUC13 plasmid as 2.2Kb PstI-BamHI DNA fragment. In order to improve the glutathione producing activity more efficiently, various recombinant plasmids containing tandem repeated gshI genes or both genes in various copy number onto the same vector were constructed. E. coli cells harboring pGH501 plasmid (pUC8-gshI$\cdot$I$\cdot$II) showed the highest glutathione synthesizing activity. The conditions for glutathione production with an ATP-generating system such as acetate kinase reaction of E. coli cells or glycolytic pathway of yeast cells were examined using the E. coli cells harboring the pGH501 plasmid. When the acetate kinase reaction of E. coli cells was used as an ATP generating system, 20mM of L-csteine was converted into glutathione with a yield of $100\%$.

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Isolation and Cultural Characteristics of a Phosphate-solubilizing Fungus, Penicillium sp. PS-113 (인산가용화 사상균, Penicillium sp. PS-113 균주의 분리 및 배양특성)

  • 강선철;최명철
    • KSBB Journal
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    • v.13 no.5
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    • pp.497-501
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    • 1998
  • A fungus with high phosphate solubilizing activites was isolated from soil using potato dextrose agar-calcium phosphate medium and identified to Penicillium sp. PS-113, based on the morphological characteristics of conidiophore and conidia; flask shape of phialide, simple branching type of conidiophore, and columnar shape of conidial head, in malt extract agar and potato dextrose agar media. The optimum temperature ad initial pH to solubilize rock phosphate in potato dextrose broth-rock phosphate medium were 30$^{\circ}C$ and pH 8.0, respectively. In these conditions phosphate solubilizing activities of Penicillium sp. PS-113 against four types of insoluble phosphate like tricalcium-phosphate, aluminium phosphate, hydroxyapatite and rock phosphate were quantitatively determined. As results, this fungus highly produced free phosphates to the culture broth with the concentrations of 1,283 ppm against tricalcium-phosphate, 585 ppm against rock phosphate, 528 ppm against aluminium phosphate, and 242 ppm against hydroxyapatite, respectively.

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