• 한국환경생태학회지
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• 제16권2호
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• pp.179-187
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• 2002

유기물이 함유된 공업용 폐수(2차처리수)의 수질을 정화하기 위해 2000년 7～10월에 친환경적인 방법인 식물정화조를 조성하여 하천의 수질 및 지하수를 보전하고 공업단지내에서 생물서식공간과 휴게공간을 제공하고자 본 실험을 진행하였다 식물정화조의 크기는 10m$\times$6m(상부면)로 1일 처리용량은 2.5㎥였다. 폐수는 1일 4회, 6시간 간격으로 0.625㎥씩 나누어 처리하였다. 처리효율을 높이기 위해 수직흐름방식을 채택하였고, 배수층은 모래와 자갈을 2:1의 비율로 혼합하여 깊이 1.0m로 조성하였다. 식물정화조 내에는 갈대, 부들, 골풀, 노랑꽃창포 등의 다년생 정수식물을 20본/㎥ 식재하였다. 처리수 재활용 차원에서 생물종다양성을 증진시키는 자연형 연못을 조성하였으며, 이를 환경교육장으로 활용하기 위해 관찰데크, 안내해설판 등을 도입하였다. 실험결과 BOD$_{5}$, COD, T-N, T-P 등의 제거에 상당한 효과가 있는 것으로 나타났고, 생태계 모니터링 결과, 생물서식공간(비오톱)으로서의 기능이 점진적으로 향상되고 있는 것으로 나타났다.

• 한국약용작물학회지
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• 제25권1호
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• pp.1-9
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• 2017

Background: Some plants have harmful effects on fungi and bacteria as well as other plants. Incorporating such plant into soil as green manure is effective in reducing population densities of soil pathogens. Methods and Results: Twenty-three species of green manure crops were cultivated after the harvest of 6-year-old ginseng and then incorporated into the soil at the flowering stage. The following year, the root rot ratio of 2-year-old ginseng and soil chemical properties were investigated. In the absence of green manure addition, the $NO_3$ content, electric conductivity (EC), and K content decreased by 95%, 79% and 65%, respectively. In the presence of green manure addition, $P_2O_5$ and $NO_3$ contents reduced by 41% and 25%, respectively. The "survived root ratio" of 2-year-old ginseng significantly increased by 56.2%, 47.5%, and 47.3%, in the Sorghum sudanense, Ricinus communis and Helianthus tuberosus treatment, respectively. In addition, there was a significant increase in the "survived root ratio" in the Secale cereale, Chrysanthemum morifolium, Atractylodes macrocephala, and Smallanthus sonchifolius treatments. The "survived root ratio" of ginseng showed a significant positive correlation with the soil pH and a negative correlation with the $NO_3$ contents, and EC. Conclusions: Cultivation of plant form the Chrysanthemum family as green manure, using mainly the rhizomes was effective for the control of root rot disease of ginseng.

• 한국습지학회지
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• 제5권2호
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• pp.57-65
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• 2003

This study was corried out to provide basic data for water analysis biodiversity, Actual vegetation map, flora and fauna on the Sanchon-tideland(Geojaesi), Doma-tideland(Namhaegun) in Kyungsangnamdo from august 2002 to February 2003. This tidelands was very broad and was being reclaimed. Therefore, it was anxious about destroying the habitat of the water birds. Since the water temperature was influenced by the surrounding air, it was highest in summer and lowest in winter. pH value and DO concentration of the water was highest in winter in the Sanchon-tideland and in surmmer in the Doma-tideland. COD concentration was lowest in winter in two tidelands. SS concentration was highest in summer. T-P and T-N concentration were very low but decreased with the seasonal change from summer to winter. Vascular plants of this area consist of 11 varieties, 67 species, 64 genera, 29 families(Sanchon-tideland) and 1 forma, 11varieties, 62 species, 64 genera, 30 families(Doma-tideland). The predominant community of this area, Phragmites communis Community were found on the seashore. The total insect specimens were classified into 120 species, 48 families, 10 orders(Sanchon-tideland) and perdominant species were Gabala argentata(Dominance 18.78%, Diversity index 1.75, Richness index 45.78). And 91 species, 40 families, 8 orders(Doma-tideland) and perdominant species were Sternolophus (Sternolophus) rufipes(Dominance 15.47%, Diversity index 1.47, Richness index 33.66). The total Fishes were classified into 3 species, 2 families, 1 order and Amphibia were 3 species, 2 families, 1 order. And Salicomia herbacea should be worthwhile species to conserve in this area. Because the water birds lived in The Tideland Sanchon and Doma, it was considered that it was necessary to preserve them.

• 한국자원식물학회지
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• 제36권6호
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• pp.571-579
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• 2023

In this study, cryopreservation by droplet-vitrification was applied to pear (Pyrus spp.) germplasm. We focused on the development and assessment of various strategies for the selection of suitable tissue, osmoprotection, and dehydration. We also evaluated post-thaw recovery of cryopreserved explants by droplet-vitrification. Preferentially, we tested the effects of preculture and loading treatments to determine which tissues were more suitable, either the apical shoot tips or the axillary buds. Apical shoot tips showed the better regrowth rate than in vitro axillary buds. The most effective techniques for cryopreservation were as follows. Shoots from in vitro seedlings which had been cultured for about 5-6 weeks were cold-hardened at 4℃ for one week, excised shoot tips were precultured on liquid MS medium including 0.3 M sucrose for 31 hours and 0.7 M sucrose for 17 hours, osmoprotected in loading solution (LS) for 40 min, and then cryoprotected in dehydration solution (PVS3) for 90 min. In addition, we found that regrowth rates of explants on regrowth medium after exposure to liquid nitrogen (LN) were higher than those on MS medium. Results indicated that the highest regrowth percentage was 95.6% for 'Bartlett' cultivar and 68.9% for 'BaeYun No.3' cultivar. Consequently, apical shoot tips of two pear cultivars, 'Bartlett' (P. communis) and 'BaeYun No.3' (P. pyrifolia), were successfully cryopreserved by droplet-vitrification. Results of this study show that the enhanced droplet-vitrification method described in the present study could be used as an effective means for long-term storage of pear genetic resources.

• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1308-1318
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• 2010

The purpose of this study was to evaluate the effect of replacing soybean meal with treated castor meal with (CMT) or without lime (CMNT) on the nutrient intake, performance, carcass characteristics, and yield of commercial cuts of beef cattle from a feedlot. Thirty male, castrated, crossbreed zebu cattle were used in the study, with an average initial weight of $360{\pm}30.27\;kg$. Five animals were used as a control group and were slaughtered at the beginning of the experiment; the remaining animals (n = 25) were distributed in random blocks (repetitions), with body weight as the criterion for block assignment. The animals were fed a diet containing 65% corn silage and 35% of concentrate on dry matter (DM) basis. Five diets consisted of four levels of soybean meal (SM) substituted with CMT (0, 33, 67 and 100%) on a DM basis and a diet with 100% of SM replaced with CMNT. At the end of the experiment, all animals were slaughtered, and their gastrointestinal tracts were emptied to determine their empty body weights (EBW). No significant effects were observed (p>0.05) for the substitution of soybean meal with CMT on intake of dietary nutrients, the average daily body weight gain (ADG) or EBW gain (EBWG). In spite of greater (p<0.05) ricin intake for the diet containing CMNT (3.06 mg/kg BW) compared to the CMT diet (0.10 mg/kg BW/d), there were no effects (p>0.05) on intake of dietary nutrients, ADG or EBWG. The average intake of DM and the ADG were 10,664.63 and 1,353.04 g/d, respectively. Regarding carcass characteristics, only carcass yield in relation to body weight was linearly reduced (p<0.05) upon substitution of SM by CMT. There was no effect (p>0.05) of the substitution of SM by CMT or CMNT on the yield of carcass basic cuts. CMT prices that are higher than 85% of the SM price do not economically justify the use of CMT. For CMT prices between 20 and 80% of the SM price, the optimal level was 67% substitution, while for prices below 15% of the SM price, the optimal level was 100% substitution with CMT. It can be concluded that treated castor meal with 6% lime can totally replace soybean meal in beef cattle diets.

• 한국약용작물학회지
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• 제22권5호
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• pp.339-348
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• 2014

The transcriptomes of four ginseng accessions such as Cheonryang (Korean ginseng cultivar), Yunpoong (Korean ginseng cultivar), G03080 (breeding line of Korean ginseng), and P. quinquefolius (American ginseng) was characterized. As a result of sequencing, total lengths of the reads in each sample were 156.42 Mb (Cheonryang cultivar), 161.95 Mb (Yunpoong cultivar), 165.07 Mb (G03080 breeding line), and 166.48 Mb (P. quinquefolius). Using a BLAST search against the Phytozome databases with an arbitrary expectation value of 1E-10, over 20,000 unigenes were functionally annotated and classified using DAVID software, and were found in response to external stress in the G03080 breeding line, as well as in the Cheonryang cultivar, which was associated with the ion binding term. Finally, unigenes related to transmembrane transporter activity were observed in Cheonryang and P. quinquefolius, which involves controlling osmotic pressure and turgor pressure within the cell. The expression patterns were analyzed to identify dehydrin family genes that were abundantly detected in the Cheonryang cultivar and the G03080 breeding line. In addition, the Yunpoong cultivar and P. quinquefolius accession had higher expression of heat shock proteins expressed in Ricinus communis. These results will be a valuable resource for understanding the structure and function of the ginseng transcriptomes.

• 한국습지학회지
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• 제16권3호
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• pp.355-362
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• 2014

하구에서의 퇴적과 침식 현상에 의한 토양특성의 변화는 식물성장 환경의 변화를 유도하고 하구 식물의 분포와 성장에 영향을 미치게 되어 다양한 생태적 기능을 수행하고 있는 하구의 주요 서식처의 변화를 초래할 수 있다. 본 연구에서는 낙동강 하구 지역의 주요 사주섬 및 서식지별 토양 특성을 파악하고자 을숙도 하단, 맹금머리등, 백합등, 도요등의 갈대와 새섬매자기 우점지역 그리고 갯벌 지역의 표토 특성을 분석하였다. 분석결과 직간접적으로 식물의 영향을 받는 용적밀도, pH, 유기물 특성 및 총질소 등에서 95% 유의수준에서 서식지 별 차이가 있는 것으로 나타났지만, 주로 입도 특성때문에 사주섬 간에 차이를 나타내었던 수분함량이나 산화환원전위 등은 큰 차이를 보이지 않았다. 이는 낙동강 하구 사주 지역 토양이 퇴적과 같은 지형 형성 기작에 주로 영향을 받으며 이후 식생의 유무나 종류 등의 영향 또한 받고 있기 때문으로 추정된다. 조사토양의 물리적 특성 중에서는 수분함량과 용적밀도, 화학적 특성에서는 유기물 함량과 pH가 각각 7개의 다른 토양특성들과 90% 이상의 유의수준에서 상관관계를 갖고 있는 것으로 나타나 대상지역의 전반적인 토양특성에 중요한 역할을 미치고 있는 것으로 나타났다.

• 식물병연구
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• 제29권4호
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• pp.440-444
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• 2023

2021년 8월 부산광역시 강서구에서 아주까리의 잎과 줄기에 점무늬병의 발생이 확인되었다. 아주까리 분리균은 tryptic soy agar에서 점액성이 없고 연한 녹색을 띠는 둥근 콜로니를 형성하였다. 세균은 단극성으로 다수의 편모를 가진 막대 모양이었다. 세균을 건전한 아주까리 유묘에 접종했을 때, 동일한 점무늬 증상의 괴사 반응이 나타났다. 병징이 나타난 부위에서 세균을 재분리하여 동일균 여부를 확인하였다. 분리균은 감자 무름 반응과 D-fucose 대사반응이 양성이었으며, troleandomycin에서 생장할 수 있었다. 16S rRNA와 항존유전자(gyrB, rpoD) 염기서열의 상동성을 비교하였을때, Pseudomonas capsici와 유연관계가 가장 높았다. 결과적으로 아주까리에서 분리된 균은 배양적, 생화학적, 유전적 특성에 의해 P. capsici로 동정되었다. 본 연구는 국내 아주까리에서 P. capsici에 의한 점무늬병의 최초 보고이다.

• Journal of Plant Biotechnology
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• 제42권4호
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• pp.290-297
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• 2015

배나무는 원산지와 분화방향에 따라 유럽, 미국, 호주 등에서 주로 재배되는 서양배와 중국, 일본, 한국 등 동남 아시아 지역을 중심으로 분포 및 재배되고 있는 동양배로 구분된다. 17개의 기본염색체를 가진 배나무는 대부분 이배성(2n=2x=34)이며, 단일 S 유전자좌에 의해 조절되는 자가불화합성과 과수 작물의 주요 특징인 유년성으로 인해 유전 연구 및 정밀한 품종 육성에 큰 제한을 받고 있다. 배나무속 식물의 유전연구는 분자생물학 관련 기술의 발달로 다양한 형태의 분자 표지의 개발이 이루어짐과 동시에 유연관계분석, 유전자지도작성, QTL 분석과 같은 다양한 유전연구에 활발히 이용되었다. 또한 배나무의 유전자지도는 병 저항성이나 다양한 유용형질과 연관된 QTL 확인을 위한 연구로 이어지고 있다. 대량 병렬 반응 및 다중처리를 토대로 획기적인 염기서열 분석 비용의 감소를 이뤄낸 NGS 기술은 대용량, 고효율, 저비용으로 식물 유전체 해독을 가능하게 하여, 중국배 'Danshansuli'와 유럽배 'Bartlett'에서 유전체 분석이 완료되었다. 최근 국내에서는 황금배, 청실리 및 미니배의 resequencing 및 GBS를 통한 SNP 탐색 등의 연구를 통해 화기, 숙기 당도 등 농업적으로 유용형질에 대한 게놈전체 연관분석을 수행하고 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권1호
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• pp.104-115
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• 2003

A series of experiments was carried out to determine the possibility for the non-ionic surfactant (NIS) as a feed additive for ruminant animals. The effect of the NIS on (1) the enzyme distribution in the rumen fluids of Hereford bulls, (2) the growth of pure culture of rumen bacteria and (3) rumen anaerobic fungi, (4) the ruminal fermentation characteristics of Korean native cattle (Hanwoo), and (5) the performances of Holstein dairy cows were investigated. When NIS was added to rumen fluid at the level of 0.05 and 0.1% (v/v), the total and specific activities of cell-free enzymes were significantly (p<0.01) increased, but those of cell-bound enzymes were slightly decreased, but not statistically significant. The growth rates of ruminal noncellulolytic species (Ruminobacter amylophilus, Megasphaera elsdenii, Prevotella ruminicola and Selenomonas ruminantium) were significantly (p<0.01) increased by the addition of NIS at both concentrations tested. However, the growth rate of ruminal cellulolytic bacteria (Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens and Butyrivibrio fibrisolvens) were slightly increased or not affected by the NIS. In general, NIS appears to effect Gram-negative bacteria more than Gram-positive bacteria; and non-cellulolytic bacteria more than cellulolytic bacteria. The growth rates of ruminal monocentric fungi (Neocallimastix patriciarum and Piromyces communis) and polycentric fungi (Orpinomyces joyonii and Anaeromyces mucronatus) were also significantly (p<0.01) increased by the addition of NIS at all concentrations tested. When NIS was administrated to the rumen of Hanwoo, Total VFA and ammonia-N concentrations, the microbial cell growth rate, CMCase and xylanase activities in the rumen increased with statistical difference (p<0.01), but NIS administration did not affect at the time of 0 and 9 h post-feeding. Addition of NIS to TMR resulted in increased TMR intake and increased milk production by Holstein cows and decreased body condition scores. The NEFA and corticoid concentrations in the blood were lowered by the addition of NIS. These results indicated that the addition of NIS may greatly stimulate the release of some kinds of enzymes from microbial cells, and stimulate the growth rates of a range of anaerobic ruminal microorganisms, and also stimulate the rumen fermentation characteristics and animal performances. Our data indicates potential uses of the NIS as a feed additive for ruminant animals.