• Microbiology and Biotechnology Letters
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• v.32 no.2
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• pp.110-116
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• 2004

The over-expression in E. coli of the pHLN1-SO(＋) and pHLN2-80(－) plasmids cloned an insecticidal crystal protein (ICP) gene (crylAal type) from Bacillus thuringiensis var. kurstaki HD 1 was investigated through in part, the deletion of -80 bp promoter and an alternative change of cloning vector system. Two recombinant plasmids were constructed in an attempt to analyze the over-expression of the ICP in relations to its gene structure possessing only -14 bp ［Shine-Dalgarno (SD) sequence of -80 bp promoter］. Also, anther two recombinant plasmids similarly cloned the icp gene in a different vector system. The amounts of ICP produced from the recombinants were measured by SDS-PAGE and confirmed by Western blot analysis. One clone, pHLRBS1-14 clone in which only the SD sequence in the inverted orientation icp gene appeared, was more evident than the pHLRBS2-14 clone in which only the -14 bp SD sequence of the right orientated icp gene was shown to exist. The pHLN2-80(－) clone produced more ICP proteins than the pHLRBS1-14 clone. In the two clones, pHLNUC1-80 right-oriented icp gene and the pHLNUC2-80 clone inverted-orientation icp gene in a new different vector, the pHLNUC2-80 produced more ICP proteins in E. coli system. These results indicate that the P/ac promoter, the inverted icp gene insertion and -80 bp promoter (-66 bp part of the icp gene promoters), were concerned with the expression of the icp gene in the recombinant plasmids. In addition, the expression mechanism might result from the disruption of the transcription-suppressing regions in the promoter regions.

• Journal of the Korean Society for Marine Environment & Energy
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• v.15 no.4
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• pp.281-291
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• 2012

In this study, WET (whole effluent toxicity) test with Skeletonema costatum, Tigriopus japonicus and Paralichthys olivaceus and ERA (environmental risk assessment) were conducted to assess the unacceptable effect on marine ecosystem by emitting the treated discharge water from 'ARA Plasma BWTS' BWMS (ballast water management system) using filtration, Plasma and MPUV module. 34 psu treated discharge water from ARA Plasma BWTS shown slight chronic toxicity effect on the P. olivaceus ($7d-LC_{50}{\Rightarrow}100.00%$ treated discharge water, $7d-LC_{25}{\Rightarrow}85.15%$ treated discharge water). Bromobenzene, chlorobenzene and 4-chlorotoluene in 34 psu treated discharge water from ARA Plasma BWTS were higher than in the background original content of seawater. The PECs (predictive environmental concentrations) of bromobenzene, chlorobenzene and 4-chlorotoluene calculated by MAMPEC (marine antifoulant model to predict environmental concentrations) program (ver. 3.0) were 3.34E-03, 2.10E-03 and 1.73E-03 ${\mu}g\;L^{-1}$, respectively and PNECs (predicted no effect concentrations) of them were 1.6, 0.5 and 1.9 ${\mu}g\;L^{-1}$. The PEC/PNEC ratio of bromobenzene, chlorobenzene and 4-chlorotoluene did not exceed one and 3 substances did not consider as persistence, bioaccumulative and toxic. Therefore, it was suggested that treated discharge water from ARA Plasma BWTS did not pose unacceptable effect on marine ecosystem.

• KSBB Journal
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• v.20 no.3
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• pp.220-227
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• 2005

Doxorubicin is an anthracycline-family polyketide compound with a very potent anti-cancer activity, typically produced by Streptomyces peucetius. To understand the potential target biosynthetic genes critical for the doxorubicin everproduction, a doxorubicin-specific DNA microarray chip was fabricated and applied to reveal the growth-phase-dependent expression profiles of biosynthetic genes from two doxorubicin-overproducing strains along with the wild-type strain. Two doxorubicin-overproducing 5. peucetius strains were generated via over-expression of a dnrl (a doxorubicin-specific positive regulatory gene) and a doxA (a gene involved in the conversion from daunorubicin to doxorubicin) using a streptomycetes high expression vector containing a strong ermE promoter. Each doxorubicin-overproducing strain was quantitatively compared with the wild-type doxorubicin producer based on the growth-phase-dependent doxorubicin productivity as well as doxorubicin biosynthetic gene expression profiles. The doxorubicin-specific DNA microarray chip data revealed the early-and-steady expressions of the doxorubicin-specific regulatory gene (dnrl), the doxorubicin resistance genes (drrA, drrB, drrC), and the doxorubicin deoxysugar biosynthetic gene (dnmL) are critical for the doxorubicin overproduction in S. peucetius. These results provide that the relationship between the growth-phase-dependent doxorubicin productivity and the doxorubicin biosynthetic gene expression profiles should lead us a rational design of molecular genetic strain improvement strategy.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.1303-1306
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• 2005

The mechanical properties of bellows, such as the extensibility and the strength can be changed depending on the shape. For the shipbuilding material, it is favorable that the fatigue life is long due to the elastic property and the reduction of thermal stress in piping system. Nowadays, the domestic production and design of bellows are based on the E.J.M.A Code. Therefore, the design standard is in need because of much errors and lack of detailed analysis. In this study, it is attempted to find out the optimal shape of U-type bellows using the finite element method. The effective factors, mountain height, length, thickness, and number of mountains and the length of joint are considered and the proper values are chosen for the simulation. The results shows that if the number of mountains are reduced, the volume decreases while the stress increases. However, the number of mountains are increased, the volume increases above the standard volume and the stress obviously increases. In addition, the effect of the thickness of bellows on the stress is very large. Both of the volume and stress are decreasing at a certain lower value region.

• The Korean Journal of Physiology and Pharmacology
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• v.5 no.6
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• pp.511-519
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• 2001

Nimopidine, one of dihydropyridine derivatives, has been widely used to pharmacologically identify L-type Ca currents. In this study, it was tested if nimodipine is a selective blocker for L-type Ca currents in sensory neurons and heterologous system. In mouse dorsal root ganglion neurons (DRG), low concentrations of nimodipine $(<10\;{\mu}M),$ mainly targeting L-type Ca currents, blocked high-voltage-activated calcium channel currents by ${\sim}38%.$ Interestingly, high concentrations of nimodipine $(>10\;{\mu}M)$ further reduced the 'residual' currents in DRG neurons from ${\alpha}_{1E}$ knock-out mice, after blocking L-, N- and P/Q-type Ca currents with $10\;{\mu}M$ nimodipine, $1\;{\mu}M\;{\omega}-conotoxin$ GVIA and 200 nM ${\omega-agatoxin$ IVA, indicating inhibitory effects of nimodipine on R-type Ca currents. Nimodipine $(>10\;{\mu}M)$ also produced the inhibition of both low-voltage-activated calcium channel currents in DRG neurons and ${\alpha}_{1B}\;and\;{\alpha}_{1E}$ subunit based Ca channel currents in heterologous system. These results suggest that higher nimodipine $(>10\;{\mu}M)$ is not necessarily selective for L-type Ca currents. While care should be taken in using nimodipine for pharmacologically defining L-type Ca currents from native macroscopic Ca currents, nimodipine $(>10\;{\mu}M)$ could be a useful pharmacological tool for characterizing R-type Ca currents when combined with toxins blocking other types of Ca channels.

• Journal of Environmental Science International
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• v.31 no.7
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• pp.555-567
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• 2022

With the introduction of the tele-monitoring system (TMS) in South Korea, monitoring of the concentration of pollutants discharged from nationwide water quality TMS attachments is possible. In addition, the Ministry of Environment is implementing a smart sewage system program that combines ICT technology with wastewater treatment plants. Thus, many institutions are adopting the automatic operation technique which uses process operation factors and TMS data of sewage treatment plants. As a part of the preliminary study, a multilayer perceptron (MLP) analysis method was applied to TMS data to identify predictability degree. TMS data were designated as independent variables, and each pollutant was considered as an independent variables. To verify the validity of the prediction, root mean square error analysis was conducted. TMS data from two public sewage treatment plants in Chungnam were used. The values of RMSE in SS, T-N, and COD predictions (excluding T-P) in treatment plant A showed an error range of 10%, and in the case of treatment plant B, all items showed an error exceeding 20%. If the total amount of data used MLP analysis increases, the predictability of MLP analysis is expected to increase further.

• Journal of Korean Society of Steel Construction
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• v.19 no.5
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• pp.493-501
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• 2007

Recently, in the domestic amount of materials,curtailment and economic efficiency security by purpose, tapered beam application is achieved, but the architectural design technology of today based on the material non-linear method does not consider solutions to problems such as brittle fracture. So, geometric non-linear evaluation thatincludes initial deformation, width-thickness ratio, web stiffener and unbraced length is required. Therefore, in this study, we used ANSYS, a proven finite elementanalysis program,and material and geometric non-linear analysis to study existing and completed tapered H-section as deep beam's analysis model. Main parameters include the width-thickness ratio of web, stiffener, and flange brace, with the experimental result obtained by main variable buckling and limit strength evaluation. We made certain that a large width-thickness ratio of the web decreases the buckling strength and short unbraced web significantly improves ductility.

• Proceedings of the KIPE Conference
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• 2004.07a
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• pp.147-150
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• 2004

Maximum power point tracking for PV systems traditionally uses either perturbation and observation method or incremental conductance method. Both methods require modulation of the output voltage and this leads to significant power loss. In this paper, a method, which senses output circuit voltage and short circuit current and use the above two parameters for optimum control with a fuzzy controller, is introduced. The short circuit current of PV cell represents illumination, and the output circuit voltage carry on information about the temperature. PCS(power conditioning system) is controlled not only feed to inverter for stability voltage variation despite of variety external environment, but also operate in order to feeding voltage and current at maximum power point by boost type chopper.

• Proceedings of the Korea Electromagnetic Engineering Society Conference
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• 2003.11a
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• pp.594-598
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• 2003

In this paper, we analyze the performance of UWB(Ultra-WideBand) communication system employing Bi-phase modulation and RAKE Receiver under the MAI(Multiple Access Interference) and the OSI(Other System Interference) environment. Using the multi-path channel model recommended by IEEE P802.15.TG3a, the performance degradation Is described with the number of users, the number of RAKE fingers and training sequences. To meet BER 10e-4 for 20 users at the same time, the number of RAKE fingers are proposed from 3 to 32. And the number of training sequences are limited less than 8 to keep the channel estimation error within 3dB

• Structural Engineering and Mechanics
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• v.31 no.5
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• pp.481-506
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• 2009

The present study is concerned with the stochastic linear free vibration study of laminated composite plate embedded with piezoelectric layers with random material properties. The system equations are derived using higher order shear deformation theory. The lamina material properties of the laminate are modeled as basic random variables for accurate prediction of the system behavior. A $C^0$ finite element is used for spatial descretization of the laminate. First order Taylor series based mean centered perturbation technique in conjunction with finite element method is outlined for the problem. The outlined probabilistic approach is used to obtain typical numerical results, i.e., the mean and standard deviation of natural frequency. Different combinations of simply supported, clamped and free boundary conditions are considered. The effect of side to thickness ratio, aspect ratio, lamination scheme on scattering of natural frequency is studied. The results are compared with those available in literature and an independent Monte Carlo simulation.