• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.454-460
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• 2009

Phyllanthus urinaria was an important species in Korea and distributed in all around of Korea. The roots and stems of this plant have been used for natural medicine for the treatment of diabetes, the hepatitis B virus and disturbances of the kidney and urinary bladder. Production of adventitious roots in P. urinaria by in vitro cultures could be used as alternatives materials. Shoot and root segments from P. urinaria seedling were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/L IBA and 30 g/L sucrose. After 4 weeks of culture, the highest induction of adventitious roots was obtained from the shoot part. Frequency of adventitious root formation on medium with various kinds of auxins (IAA, NAA, 2,4-D, and IBA) and various concentrations of IBA (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L) was tested. The maximun induction of adventitious root was obtained on medium with 0.5 mg/L IBA. In liquid culture, growth of root was best on medium supplemented with 30 g/L sucrose. Adventitious roots were cultured in 5 L bioreactor containing 1/2 MS medium supplemented with 0.5 mg/L IBA and 30 g/L sucrose and mass-production of adventitious roots was successfully achieved. These results revealed the first attempt for the production of adventitious roots in P. urinaria.

• International Journal of Industrial Entomology and Biomaterials
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• v.20 no.1
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• pp.13-17
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• 2010

Ten numbers of plants, based on their medicinal value, were used to test their efficacy against virosis (caused by cytoplassmic polyhedrosis virus) in tasar silkworm, Antheraea mylitta Drury. in indoor rearing conditions. The aqueous extracts of leaf of Azadirichita indica (neem), Acharanthus aspera, Psoralea corylifolia, Asparagus racemosus, Adhatoda zelanica (Basak), Andrographis paniculata (Kalmegh), Moringa oilephera (sahjan), whole plant of Phyllanthus urinaria (Bhuiamla), Centella asiatica (Veng sag) and Curcuma longa (Haldi powder) in different concentrations were used for containment of virosis in silkworm larvae. The tasar silkworm larvae were orally inoculated with PIBs ($1\times10^6$) of AmCPV in $2^{nd}$ instar and treated with plant extracts in each instar ($2^{rd}$ instar onwards). The mortality due to virosis was recorded during larval period. The plant extracts, irrespective of their concentrations, were found effective in suppressing the virosis where P. urinaria reduced the virosis to 56.90% followed by A. paniculata (53.82%) and least in C. asiatica (5.15%). The lowest pooled larva mortality 36.99% was recorded in the treatment of P. urinaria. Comparatively higher larva mortality 39.91% was observed with the treatment of A. paniculata. The highest larva mortality in treatment was with C. asiatica (81.99%). In treated control larva mortality was 86.50%.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.59-66
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• 2018

Propionibacterium acnes, a major pathogens bacterium of acne, is one of the microflora associated with the inflammation. In this study, the antibacterial effects of 70% ethanol extracts from the 61 natural plants in Jeju against P. acnes were investigated using the disc diffusion method. The 45 natural plant extracts showed antibacterial effects and the 16 natural plants extracts didn't show antibacterial effects. The Phyllanthus urinaria L. (stem and leaves) extract showed thr highest antimicrobial activity with $18.96{\pm}0.69mm$ clear zone of the growth of P. acnes, followed by Angelica gigas Nakai (roots), Gynostemma pentaphyllum (Thunb.) Makino, Angelica gigas Nakai (stem and leaves), Isodon inflexus (Thunb.) Kudo, camellia sinensis L. (flowers), Rosmarinus officinalis, etc. These results suggest that the natural plant in Jeju can be used as functional cosmetic biomaterials or antimicrobial agents against P. acnes.