• Mycobiology
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• v.40 no.4
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• pp.231-235
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• 2012

Fungal endophytes are microfungi that live in plants without causing apparent symptoms of infection. This study was conducted to identify endophytic fungi isolated from leaves of coniferous trees in Bohyeon Mountain of Korea. We collected leaves of two species of coniferous trees, Pinus densiflora and Pinus koraiensis, from 11 sites in the study area. A total 58 isolates were obtained and identified using molecular and morphological characteristics. Four species of endophytic fungi were isolated from P. densiflora: Lophodermium conigenum, Leotiomycetes sp., Septoria pini-thunbergii, and Polyporales sp., while two fungal species were isolated from P. koraiensis: Eurotiomycetes sp. and Rhytismataceae sp. The most frequently isolated species were L. conigenum and S. pini-thunbergii.

• Journal of Korean Society of Forest Science
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• v.87 no.4
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• pp.557-561
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• 1998

A die-back fungus isolated from Pinus koraiensis was identified as Cenangium ferruginosum. Mycelial growth of the fungus was the best on PDA among five media tested. Mycelial growths on agar media supplemented with extracts from coniferous trees were relatively better than those on media with extracts from non-coniferous trees. Optimum temperature and pH for the mycelial growth of C. ferruginosum on PDA were $10^{\circ}C$ and pH 5, respectively. When five-years-old P. koraiensis seedlings were artificially inoculated with the fungus for the pathogenicity test of this fungus, 80% of seedlings were infected. and the same symptoms and signs were developed as those of naturally-infected trees.

• Journal of the Korea Furniture Society
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• v.20 no.4
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• pp.281-289
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• 2009

This research examined the technical feasibility of composite that had two layers of fiberglass reinforcement to enhance the load carrying capacity of lumber. Specimens were prepared from standard lumber made of thinning crop trees such as Larix kaempferi, Pinus koraiensis, and P. rigida. Results indicated that bending properties, hardness, and wood screw withdrawal load of low density lumber from the P. koraiensis increased as the number of layers of fiberglass reinforcement increased. Composite produced by VARTM method showed the MOE increased in all specimens, while the MOR differed on the location, whether the 2-layers of FRP is put on tensile zone or compressive zone. The MOR of high density lumber from Larix kaempferi and P. rigida decreased when they placed into tensile zone, but low density lumber from P. koraiensis increased in same location. The bending toughness only increased high density lumber of Larix kaempferi as the number of layers of fiberglass reinforcement increased. The technique developed by this study may increase an opportunity to use small diameter log with low density for commercial purposes.

• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.353-358
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• 2010

In this study, we investigated the relationship of tree-ring growth with total nitrogen content, and stable carbon and nitrogen isotopes from the core samples of Pinus koraiensis (35-year-old). Annual ring width showed significant positive correlations with the ${\delta}^{13}C$ (P=0.003). The total nitrogen content (P=0.024), and the ${\delta}^{13}C$ content was also correlated with total nitrogen content (P=0.038), indicating that the growth of P. koraiensis was stimulated as the contents of both ${\delta}^{13}C$ and total nitrogen were increased. On the other hand, the less the ${\delta}^{15}N$ content and the C/N ratio were, the larger the annual ring width was. Moreover the families with relatively better growth performance contained the higher levels of ${\delta}^{13}C$ in the xylem compared to other families. These results suggest that the ${\delta}^{13}C$ and total nitrogen contents are the important determinants in the growth performance of P. koraiensis.

• Journal of Korean Society of Forest Science
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• v.89 no.3
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• pp.422-430
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• 2000

Three-year-old Pinus koraiensis seedlings, transplanted in brown forest soils originating from granite were treated with simulated acid rain of pH concentrations 5.6(control), 4.0, 3.0, 2.5 and 2.0 for 210 days from April 21 to November 17, 1999. Visible injury of the seedlings were observed at the pH 2.0 and pH 2.5 treatments. The total dry weight of the seedlings decreased at pH 2.0 treatment compared with that of the control, and T/R ratio increased at pH 2.0 treatment compared with others. The elements in each part of the seedlings, concentrations of Ca, P and content of chlorophyll in needles increased at the pH 2.0 treatment compared with the control. The concentration of N in the needles of the seedlings increased as the soil pH decreased. As the treated pH was lowered, soil pH has decreased, and concentrations of Ca, Mg, Al, and Mn increased, especially at pH 4.4. In addition, there was a strong correlation(r=0.90, p<0.05 ; r=-0.94, p<0.01) between the dry weight of the seedlings and the pH and Al concentration of the soils. Therefore, the pH and Al concentration in the soil may be useful indicator for assessing the effect of acid rain on the growth of woody plants.

• The Plant Pathology Journal
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• v.16 no.1
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• pp.52-54
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• 2000

Pitch canker of Pinus rigida, caused by Fusarium subglutinans f. sp. pini, was first noticed in Inchon, Korea, and is now being spread into other plantations of pines. Typical symptoms re resin flows from canders on the shoots, branches, and trunks, and resin-soaking of the sap wood under the bark. Pathogenic fungi were isolated from the infected shoots, branches, and sees on Fusarium-selective medium. Mycelial growth and microscopic characteristics were examined. Pathogenicity test was carried out by inoculating four common species of pines (P. rigida, P. densiflora, P. thunbergii, and P. koraiensis) in Korea. P. rigida and P. thunbergii showed symptoms identical to those of naturally infected trees, while P. densiflora and P. koraiensis remained free symptomless.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.497-502
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• 2008

The essential oil from the cones of Pinus koraiensis was prepared after removing the seeds, and its chemical composition analyzed using gas chromatography-mass spectrometry (GC-MS). Hydrodistillation of the P. koraiensis cones yielded 1.07% (v/w) of essential oil, which was almost three times the amount of essential oil extracted from the needles of the same plant. Moreover, the antimicrobial activities of the oil against the growth of Gram-positive bacteria, Gram-negative bacteria, and fungi were evaluated using the agar disc diffusion method and broth microdilution method. Eighty-seven components, comprising about 96.8% of the total oil, were identified. The most abundant oil components were limonene (27.90%), ${\alpha}$-pinene (23.89%), ${\beta}$-pinene (12.02%), 3-carene(4.95%), ${\beta}$-myrcene (4.53%), isolongifolene (3.35%), (-)-bornyl acetate (2.02%), caryophyllene (1.71%), and camphene (1.54%). The essential oil was confirmed to have significant antimicrobial activities, especially against pathogenic fungal strains such as Candida glabrata YFCC 062 and Cryptococcus neoformans B 42419. Therefore, the present results indicate that the essential oil from the cones of Pinus koraiensis can be used in various ways as a nontoxic and environmentally friendly disinfectant.

• Journal of Korean Society of Forest Science
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• v.61 no.1
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• pp.15-19
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• 1983

To establish the stand volume table of Pinus koraiensis S. et Z. and Larix leptolepsis Gord. mean diameter at breast height (D), mean height, basal area per ha, stand from height (H.F), basal area height, number of trees per ha, volume per ha, stand age and site were investigated for 107 plots of P. koraiensis and 82 plots of L. leptolepsis throughout the country. The obtained results are as follows; 1) the relationships between stand volume (V) and H.F. were log V=1.3855+0.1168 H.F and log V+0.9929+0.1543 H.F in P. koraiensis and L. leptolepsis, respectively. 2) The relationship between D and H.F was estimated as H.F = 1.2569+0.2576D for P. koraiensis and as H.F = 4.3045+0.1443D for L. leptolepsis 3) Estimated errors calculated with the application of these stand volume tables were 18.29% and 19.22%r for P. koraiensis and L. leptolepsis respectively.

• Journal of Korean Society of Forest Science
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• v.69 no.1
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• pp.13-18
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• 1985

Forest soils mixed with organic matters (green needle, flesh needle litter and needle litter in F layer of Pinus koraiensis, and green leaf of Quercus dentata and Q. variabilis) were incubated under a constant $30^{\circ}C({\pm}1)$ for 53 days to measure the changes of inorganic nitrogen and $CO_2$ evolution rate. The results obtained were summarized as follows; 1) In the early incubation period the amounts of total inorganic nitrogen in soils by mixture of organic matters decreased rapidly because of immobilization by microbial uptake, and thereafter their amounts increased with further incubation. 2) The rate of immobilization of organic nitrogen in mixed organic matters was the highest in green needle among green needle, flesh needle litter and needle litter in F layer of P. koraiensis, but lower than that of green leaf of Q. variabilis and Q. dentata. 3) The rates of $CO_2$ evolution from soils mixed with organic matters increased sharply in the early time, and then decreased slowly with increasing time. The order of the $CO_2$ evolution rate was green leaf of Q. variabilis > green leaf of Q. dentata > green needle of P. koraiensis > flesh needle litter of P. koraiensis > needle litter of P. koraiensis in F layer from the largest to the least. 4) Nitrate nitrogen concentrations showed a tendency to increase throughout incubation time, so that their concentrations after 53 days were higher than that of ammonium nitrogen.

• Journal of Korean Society of Forest Science
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• v.96 no.4
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• pp.414-418
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• 2007

The average number of oviposion scars in lower (facing the Land) and upper part (facing the Sun) of P. koraiensis logs were $16.3{\pm}13.9$ and $3.3{\pm}3.6$, respectively. Oviposition preference of Monochamus saltuarius adults was lower part of Pinus koraiensis. Estimated average number of larvae and egg from oviposition scars ($64.7{\pm}23.8$) in P. koraiensis logs was $18.8{\pm}10.0$ in the laboratory condition. However, oviposition scars were $63.6{\pm}31.1$ and average number of larvae and egg was $2.0{\pm}2.6$ in field condition. When we fed with inner bark (phloem) and xylem of P. koraiensis, body weight of M. saltuarius larvae was normal. However, when we only fed xylem of P. koraiensis, body weight and size were reduced but they were recovered after feeding inner bark (phloem). Esterase isozymes were assessed from larvae fat body of M. saltuarius and Est1 were specifically activated when we fed with inner bark (phloem) and xylem.