• Journal of the Korean Institute of Landscape Architecture
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• v.17 no.3
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• pp.35-47
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• 1990

This study was executed to analyze the vegetational landscape structure and administration planning of Heonin Royal Tomb forest by three kinds of ordination techniques (PCA, RA and DCA) and comparison of the couple photographs between 1920s and 1980s. Seventeen sites in the recreation area and fifteen sites in the protective area were sampled with clumped sampling method in June 1988 and five quadrats were examined in each site. Environmental impact grade 3, 4 and 5 area covered 56.4% of the surveyed area and these area should be restored by the input of human energy. Pinus densifora community of actual vegetation covered 8.4%, Alnus japonica 24.2% and Quercus community 40.9% of the total area. And the afforested vegetation of Pinus koraiensis and Pinus rigida covered 23.1 % The recreation area was divided by P. densiflora, P. densiflora-Quercus aliena, A. japonica-Q.aliena, A. japonica and the protective area by Q. acutissima, Q. aliena, A. japonica-Q. aliena.. DCA ordination showed that successional trends of tree species seem to be from P. densiflora, Sorbus alnifolia, Styrax obassia to Q. variabilis Q. serrata in P. densiflora community and from A. japonica through A. ginnala to Q. aliena in A. japonica community of the upper layer. By the comparison of the couple photographs between 1920s and 1980s, we can recognize the change of historical landscape composed of P. densiflora community and those community is succeeded to Q. aliena.

• Korean journal of food and cookery science
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• v.25 no.4
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• pp.435-444
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• 2009

In this study, Paeonia japonica powder was extracted with ethanol, and its antimicrobial activity was investigated. The ethanol extract of the P. japonica had antimicrobial activity against Bacillus subtilis, Escherichia coli and Staphylococcus aureus. The inhibition zones of the P. japonica ethanol extract (3 mg/disc) against B. subtilis, E. coli and S. aureus were 10, 11, 8.5 mm, respectively. To test the food preservation effect of P. japonica and determine the optimal ratio of the P. japonica extract in the formulation, Sulgidduk samples were prepared with substitutions of 0, 0.25, 0.5, 0.75 and 1% P. japonica extract, and the quality characteristics of the samples were then investigated over 4 days of storage. In these experiments, total cell counts tended to decrease as the amount of added P. japonica extract increased. Moisture contents were not significantly different among the Sulgidduk samples. As the content of the P. japonica extract increased, the L-values of the samples decreased and the a- and b- values increased. In regards to the textural characteristics, the hardness, gumminess, and chewiness of the Sulgidduk samples decreased as the amount of P. japonica extract increased; however, they increased with the progression of storage time. Adhesiveness, springiness and cohesiveness were not significantly different at the different P. japonica extract concentrations and decreased with storage time. In the sensory evaluation, the control group had significantly higher scores for color, flavor and after taste as compared to the P. japonica extract added groups. When the P. japonica extract content was increased, the flavor and overall acceptability decreased, while Bakjakyak flavor, bitterness and off-flavor increased. Softness was not significantly different among the samples. In conclusion, the results indicate that substituting $0.25{\sim}1%$ P. japonica extract in Sulgidduk is optimal for quality and provides a product with reasonably high overall acceptability.

• The Journal of Internal Korean Medicine
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• v.19 no.2
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• pp.300-316
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• 1998

The various oriental herbal medicines, which have usually been used for treatment of reducing fever, purging intense heat and detoxication, were screened to determine the antibacterial activity and the minimal inhibitory concentration against antibiotics-resistant Staphylococcus aureus. The results obtained were as follows: 1. Among the 23 oriental medicines tested, the water-soluble extract of Coptis japonica showed very excellent antibacterial activity against antibiotics-sensitive and -resistant S. aureus. A little activity was found in the extracts of Scutellaria baicalensis, Eriobotrya japonica and Prunus mume against antibiotics-sensitive S. aureus and in that extracts of P. mume, Schizandra chinensis and S. baicalensis against antibiotics-resistant S. aureus. When C japonica was used, the high antibacterial activity was shown against Bacillus subtilis and other extracts showed a little or no antibacterial activity against B. subtilis and E. coli as a control. 2. The highest antibacterial activities against antibiotics-resistant S. aureus were found in the ethanol-soluble extract of C japonica. Other ethanol-soluble extracts of S, baicalensis, S. chinensis and P. mume showed a little activity against both antibiotics-sensitive and antibiotics-resistant S. aureus. 3. Among the medicinal herbs tested, the water- and ethanol extracts of C. japonica showed very excellent antibacterial activity against the pathogenic S. aureus and both Escherichia coli and Bacillus subtilis used as a control. 4. When the water-soluble extracts of C. japonica and S. baicalensis, minimal inhibitory concentrations (MICs) against antibiotics-sensitive S. aureus were $20mg/m{\ell}\;and\;30mg/m{\ell}$, respectively. The MICs of the ethanol-soluble extracts of C. japonica and P. scabriosaefolia were $7.5mg/m{\ell}\;and\;12mg/m{\ell}$, respectively. 5. For the MICs against antibiotics-resistant S. aureus, the water-soluble extracts of C. japonica and P. mume showed $15mg/m{\ell}\;and\;10mg/m{\ell}$, respectively. The MICs of the ethanol-soluble extracts of C. japonica and P. mume were $13mg/m{\ell}\;and\;20mg/m{\ell}$, respectively. As a result, the highest antibacterial activity was found in both water- and ethanol-soluble extracts of C. japonica against antibiotics-resistant S. aureus. And the water- and ethanol-soluble extracts of S. baicalensis, S. chinensis and P. mume showed comparatively high antibacterial activities against both antibiotics-sensitive and -resistant S. aureus.

• Korean Journal of Environmental Agriculture
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• v.41 no.1
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• pp.50-54
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• 2022

BACKGROUND: Phyllospadix japonica Makino is a perennial plant belonging to the family Zosteraceae. This species is native to the eastern coast of the Korean Peninsula, and it is found attached to rocks on the seashore. As with all seagrass species, P. japonica is also known to play a major role in protecting the marine environment, and studies on its physiological activities suggestive of its antioxidant, antibacterial and anti-inflammatory potentials have been reported. In this study, purification and structural analysis were performed to identify the polyphenol substances derived from P. japonica. METHODS AND RESULTS: An polyphenol substance MP-1 was purified from the 70% aqueous methanol extract of P. japonica using Diaion® HP-20 column chromatography, ODS column chromatography, and medium-pressure liquid chromatography (MPLC). The purified MP-1 was identified as rosmarinic acid having a molecular weight of 360 and a molecular formula of C18H16O8 through electrospray ionization (ESI)-mass and nuclear magnetic resonance (NMR) spectroscopic analysis. CONCLUSION(S): This study highlights the processes used for the identification of the polyphenol substance derived from P. japonica. Rosmarinic acid, the polyphenol derived from P. japonica identified by this study, is a kind of bioactive substance mainly present in plants. These findings provide an important starting point and are valuable for future studies on bioactive substances in seagrass.

• Korean Journal of Environment and Ecology
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• v.34 no.2
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• pp.157-169
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• 2020

The purpose of this study is to investigate the ecological characteristics and vegetation structures of Eurya japonica in Busan. As a result of the TWINSPAN and DCA analysis, 89 plots of 100㎡ each were divided into 3 communities: Quercus serrata-Pinus densiflora-E. japonica community, Pinus thunbergii-E. japonica community, and P. thunbergii-Camellia japonica community. Community I consisted of the Quercus serrata-Pinus densiflora-E. japonica which was mainly located in the high altitude inland. While Q. serrata and P. densiflora competed in the tree layer, the dominant species of the understory layer was E. japonica. Since Carpinus tschonoskii, one of the climax species, was distributed evenly from shrub to tree layers, it was likely that deciduous oak trees or Carpinus tschonoskii would become dominant species in community I. In community I, E. japonica was found in higher altitude than the other evergreen broad-leaved tree and was expected to maintain their tree vigor even if the vegetation structure is converted into the deciduous forest. Community II, the P. thunbergii-E. japonica community, was predicted to maintain its tree vigor unless there were unexpected disturbance factors. Community III, consisting of P. thunbergii-C. japonica and located in Dongbaek Island, was under artificial management. In community III, P. thunbergii was the only species in the tree layer, while C. japonica was predominant in the understory layer. E. japonica and various evergreen broad-leaved tree species were present in the understory layer and shrub layer, which were unmanaged areas. Therefore, it is expected that unless C. japonica is continuously managed, E. japonica is likely to become the dominant species. There were also various evergreen broad-leaved species, such as Machilus thunbergii and Pittosporum tobira, present in the shrub layer. If the temperature continues to rise, the habitat is expected to become evergreen broad-leaved forests in the future as P. thunbergii community declines. The result of Pearson's correlation coefficient analysis of E. japonica and species appearing in 89 plots showed that 9 species were had a statistically significant relationship (p<0.05). Four species, including P. tobira and Q. dentata, had a positive correlation. Five species had a negative correlation, and C. japonica, which had the same ecological position as E. japonica, showed the most negative correlation at -0.384.

• Journal of Life Science
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• v.29 no.12
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• pp.1386-1392
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• 2019

This study investigated the potential of dye plants as natural oral health products. The antibacterial activity of ethanol stem extracts of A. japonica, R. verniciflua Stokes, G. jasminoides, D. morbifera, P. amurense Rupr., and S. japonica against P. gingivalis KCTC 5352, S. mutans KCTC3065, S. downei KCTC3634, S. sanguinis KCTC3284, and S. gordonii KCTC 3286 was confirmed. Among the stem extracts from 6 dye plants grown in Korea, ethanol extract from A. japonica stem (1 mg/disc) showed the highest antibacterial activity against P. gingivalis KCTC5352. The A. japonica stem extracts showed antibacterial activity similar to chlorhexidine, which was used as a positive control. The MIC and MBC of P. gingivalis KCTC5352 were 0.4 mg/ml and 0.6 mg/ml, respectively. The biofilm production rate and cell growth of P. gingivalis KCTC5352 in the cultures treated with 0.2-2.0 mg/ml of A. japonica extract were significantly decreased in a concentration-dependent manner. In addition, the mRNA expression of the superoxide dismutase and fimA associated with fimbriae formation in these cultures was suppressed, also in a concentration-dependent manner. Based on these results, it is concluded that A. japonica stem extracts can be used as an oral health product derived from natural materials, as demonstrated by its antibacterial action against and inhibition of biofilm formation of P. gingivalis KCTC5352.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.5
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• pp.548-554
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• 2008

The aim of this study was to identify the inhibitory effect of hepatic toxicity and liver lipid metabolism after the administration of Artemisia capillaris and Paecilomyces japonica. SD rats were divided into $CCI_4$ treated group with subgroups of 6% Artemisia capillaries (6A), 4% Artemisia capillaris＋2% Paecilomyces japonica (4A2P), 3% Artemisia capillaris＋3% Paecilomyces japonica (3A3P), 2% Artemisia capillaris＋4% Paecilomyces japonica (2A4P) and 6% Paecilomyces japonica (6P). In this study we also intended to verify the optimum ratio of Artemisia capillaris and Paecilomyces japonica which can reduce hepatotoxicity. Artemisia capillaris and Paecilomyces japonica reduced cholesterol and triglyceride levels which were increased by the treatment of $CCI_4$. HDL-cholesterol level was the most enhanced in the group of 4A2P. On the other hand, athrogenic index (AI) was reduced statistically (p<0.05). When the ratio of Artemisia capillaris and Paecilomyces japonica was 2:1, the improvement of rat serum and liver lipid metabolism and the alleviation of hepatic damage induced by $CCI_4$ were shown to be the most effective in this study. It is considered that the symptoms of severe chemically induced hepatotoxicity could be lessened by Artemisia capillaris and Paecilomyces japonica administration.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.2
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• pp.71-81
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• 2016

This text was experimented and investigated the optimum production time and germination characteristics of seed that collect in Korean Chungnam, in order to offer the basic informations for slope restoration and revegetation using Poaceae plant. Optimum time of seed production was mid-late October of Miscanthus sinensis, Pennisetum alopecuroides, early November to late October of Themeda triandra var. japonica and M. sacchariflorus, and mid November of Phragmites communis. Epiphytic amount of seed full ripe was the most times in mid October of M. sinensis, P. alopecuroides and M. sacchariflorus, early November of T. triandra var. japonica, and mid November of P. communis. Seed rate of maturity was the highest times in early November of M. sinensis, P. alopecuroides and M. sacchariflorus, and mid November of T. triandra var. japonica and P. communis. Germination rate by seed collection time was the highest times in early October of M. sacchariflorus, mid October of T. triandra var. japonica, early November of M. sinensis and mid November of P. communis. Germination days by seed production time was the shortest times in early November of M. sinensis and M. sacchariflorus, mid November of T. triandra var. japonica and P. communis. Optimum temperature of germination was $20{\sim}25^{\circ}C$ in M. sinensis and P. alopecuroides, $25^{\circ}C$ in T. triandra var. japonica, and $25{\sim}30^{\circ}C$ in P. communis.

• Korean Journal of Environmental Biology
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• v.17 no.2
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• pp.153-158
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• 1999

Uptakes of N and P by Phragmites japonica Steudel. are determined in continuous flow tanks as a function of hydraulic retention time (HRT, days) and nutrient concentration (NC). Results show that the uptake was higher at shorter HRT and higher nutrient concentration, and the regression equations were estimated. Mean above ground biomass of Phragmites japonica in the middle reaches of Sagimak stream was 335.92 g/$m^2$, and estimated uptake by this vegetation were 7.31~20.15 NH$_4$-N, 31.15~95.84 NO$_3$-N, and 4.09~11.48 PO$_4$-P mg/$m^2$/day in Sagimak stream, respectively when HRT was 1~5 days. According to this study, it is concluded that Phragmites japonica Steudel. are suitable for water quality improvement of stream through nutrient uptake at short HRT and high concentration.

• KSBB Journal
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• v.29 no.1
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• pp.67-71
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• 2014

Planaria were recently reported to be a simple and sensitive model to investigate the mechanistic aspect of seizure and to screen potential anticonvulsants. Using planarian model, we analyzed the pharmacological effect of ukgansan (UGS), an oriental herbal medicine containing seven medicinal herbs, on the planarian locomotor velocity (pLMV) and glutamate-induced seizure-like activity (pSLA). To test whether D. japonica is suitable for studying anti-seizure agents, we investigated the effect of glutamate on pLMV and pSLA in D. japonica. In the present study we first confirmed that pSLA in D. japonica was induced by L-glutamate. Glutamate significantly produced pSLA in a dose dependent manner, but did not affect pLMV. These glutamate-induced paroxysms were decreased by antiepileptic drug, topiramate. A similar inhibitory effect on glutamate-induced pSLA was observed after the treatment of UGS. The present results suggest that UGS and its active constituents possess useful substance inhibiting seizure in planarian and that D. japonica provides a convenient model to search active herbs containing anti-seizure activity.