• Title/Summary/Keyword: P. florida

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Comparison of Water Relations of Three Cultivated Pleurotus Species and Trichoderma Green Moulds

  • Lee, Hyang-Burm;Naresh Magan;Yu, Seung-Hun
    • The Plant Pathology Journal
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    • v.16 no.1
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    • pp.25-28
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    • 2000
  • The effect of ionic osmotic potential (Ψ$\pi$), and matric potential (Ψm) in the range of -0.2 to -4.0 Mpa on mycelial growth of three species of Pleurotus (P.florida, P.ostrenatus and P.safor-caju) were determined over a range of temperature (15-3$0^{\circ}C$) on a 2% malt extract agar medium and compared with the Ψ$\pi$ effect on growth of two strains of Trichoderma green mould. With the ionic solute KCl, optimun Ψ$\pi$for growth was -0.2 MPa for P.floreda and in the range of -0.2 to -0.5 MPa, with slight growth at -3.0 MPa and with nogrowth at -4.0 MPa. Of the species of Pleurotus, P.florida grew signigicantly slower than the other two species. Growt of the species of Pleurocus was significantly slower when water potential (Ψ$\omega$) was modified matrically with polyethylene glycol (PEG) 8000 then osmotically with KCl. They were also more sensitive to changes in Ψm than Ψ$\pi$The optimum Ψm of the Pleurotus was -0.5 Ψm, with no growth below -3.0 MPa. Of the species of Pleurotus, P.florida was most sensitive and P.sajor-caju was more tolerent to lowered Ψ$\pi$,but P.sajor-caju was most sensitive to lowered Ψm. The growth rate of the Trichoderma green mould strains was much faster than that observed for the Pleurotus spp. Optimum growth for bot strains of Trichoderma was in the range of -0.2 to -0.5 MPa. Strain CNU 503 was more tolerant to water stress than strain CNU 501. Both strains were able to grow up to 30% of optimum growth at -4.0 MPa at 25-3$0^{\circ}C$.

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Fruiting body development and genetic analysis of somatic hybrids by protoplast fusion in edible fungi (식용버섯의 원형질체 융합체의 자실체 발생 및 유전분석)

  • Yoo, Young Bok;Kong, Won Sik;Oh, Se Jong;Jhune, Chang Sung;Shin, Pyung Gyun;Kim, Beom Gi;Kim, Gyu Hyun;Park, Minsun;Min, Byung Re
    • Journal of Mushroom
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    • v.2 no.3
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    • pp.115-126
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    • 2004
  • Somatic hybrids of inter-compatible and inter-incompatible strains were obtained by protoplast fusion. The fusion products between compatible strains, Pleurotus ostreatus and P. florida, formed heterokaryons, while fusants between incompatible strains such as P. cornucopiae + P. florida, P. ostreatus + Ganoderma applanatum, P. florida + Ganoderma lucidum, and P. ostreatus + Flammulina velutipes formed synkaryons that retained genes from both parents. The heterokaryons showed the same level of basidioma development. In contrast, the synkaryons showed unique characteristics including clamp connection formation at mitosis, either partner basidioma development, and abnormal segregation and recombination compared with inter-compatible strains. Synkaryons can be classified into homokaryoyic and heterokaryotic type. A comparison of somatic hybrids with compatible and incompatible strains was made using random amplified polymorphic DNA (RAPD) analysis. The heterokaryons between compatible species showed the same level of variability and contained both parental RAPD bands. In contrast, most of the synkaryons between incompatible species showed similarity to those of either parental bands and non-parental RAPD bands. Synkaryons can be classified into microgenome insertion type and macrogenome insertion type. A tetrapolar mating system was found among monospore isolates in somatic hybrids and wild type P. ostreatus. Homokaryons from each somatic hybrid combination were paired with tester homokaryons of the initial wild type of P. ostreatus. The changed mating types were identified in progenies. The pattern of mating type switching in somatic hybrids depends on compatibility of fusion partner. There are several factors related to the mechanism of clamp connection formation and fruiting body development of synkaryons. Of these,the major factor may be associated with self-fertility and mating type switching such as homokaryotic fruiting of wild type P. ostreatus. This review will discuss these aspects.

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Bandgap Tuning in InGaAs/InGaAsP Laser Structure by Quantum Well Intermixing

  • Nah Jongbum;Kam PatrickLi
    • Korean Journal of Optics and Photonics
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    • v.16 no.2
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    • pp.159-161
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    • 2005
  • We report the selective area bandgap tuning of multiple quantum well structures by an impurity free vacancy induced quantum well intermixing technique. A 3dB waveguide directional coupler was fabricated in the disordered section of an intermixed quantum well sample as a demonstration of photonic device applications.

Effect of Difference in Cold-tolerance of Variety on Forage Productivity of Italian Ryegrass in Middle Regions of Korea (중부지역에서 이탈리안 라이그라스 품종의 내한성 차이가 생산성에 미치는 영향)

  • Choi, Gi Jun;Choi, Ki Choon;Hwang, Tae Young;Lee, Ki Won;Kim, Ji Hye;Kim, Won Ho;Lee, Eun Ja;Sung, Kyung Il;Jung, Jeong Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.4
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    • pp.210-216
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    • 2018
  • This experiment was carried out to study the effect of difference in cold-tolerance and growth characteristics of variety on forage productivity of Italian ryegrass(IRG) in middle region of Korea from 2017 to 2018. Cold-tolerance of IRG was significant different between varieties(p<0.05). Overwinter rate of domestic variety of IRG was higher than that of foreign variety(p<0.05). Among early-maturing varieties, overwinter rate of Kowinearly was highest as 68% that was 33% higher than that of Florida 80. Among middle-maturing varieties, overwinter rate of Kowinmaster was highest as 70% that was 28% higher than that of Tam 90. DM yield of IRG was significant different between varieties(p<0.05). Among early-maturing varieties, DM yield of Kowinearly and Greenfarm 2 was about 3 to 3.5 ton/ha more than that of Florida 80. Among middle-maturing varieties, DM yield of Kowinmaster was about 2ton/ha more than that of winter-hawk or Tam 90. There is a direct correlation between forage productivity and cold-tolerance of IRG variety(p<0.01). These results suggested that choice of early- or middle-maturing variety with cold-tolerant is more important for increasing the forage productivity of IRG If harvesting for roughage have to be finished in the middle ten days of May in middle region of Korea.

Nuclear DNA inheritance of intra-specific somatic hybrids by di-mono cross in Pleurotus ostreatus based on URP-PCR analysis (URP-PCR 분석에 의한 느타리 이핵-단핵 계통간 교잡주의 핵 DNA 유전)

  • Kim, Eun Jung;Shin, Pyung Gyun;Jang, Kab Yeul;Kong, Won Sik;Han, Young Sook;Yoo, Young Bok
    • Journal of Mushroom
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    • v.12 no.2
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    • pp.96-106
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    • 2014
  • The primary objective of the present study is the characterization of the somatic hybrids of dikaryon-monokaryon (di-mono) crosses in mushroom breeding. We employed this technique for developing superior strains from Pleurotus ostreatus strains with 48 intraspecific hybrids of 12 combinations between six P. ostreatus strains and one P. florida strain. The results on the experiments of hybridization rate, nuclear DNA patterns, and colors and morphology of fruit-bodies, are presented. In di-mono crosses, somatic hybrids among Pleurotus strains showed 100% of crossability as seen in those between P. ostreatus and P. florida strains indicating that the nuclei of a dikaryon is inferred to be migrated to a recipient. 87.5% of the somatic hybrids among Pleurotus strains were similar to the donor dikaryons, and 12.5% of the somatic hybrids presented DNA patterns of both parents. In 16.6% of di-mono crosses between P. ostreatus and P. florida, the nuclear DNA patterns of all hybrids showed the same or similar patterns compared to the donor dikaryons. 70.9% of the hybrids between P. ostreatus and P. ostreatus were similar to the donor dikaryons and 12.5% of them presented the DNA patterns of both parents. 79.2% of fruiting body morphology of the hybrids among Pleurotus strains were similar to the dikaryons and 20.8% of them were similar to both parents. Interestingly, the morphology of all dikaryons were dissimilar each other. All hybrid strains between dikaryon P. florida and monokaryon P. ostreatus showed the fruiting body of which colors were similar to those of the dikaryon, while the hybrids between dikaryon P. ostreatus and monokaryon P. florida were showed the combined colors of both parents. Therefore, the fruiting body color of P. florida tends to be generally dominant. In conclusion, the present study provides a way to find out and suggest superior hybrid strains using the nuclear DNA patterns of hybrids between Pleurotus strains as well as the characteristics of their fruiting bodies. The advantages of the di-mono crossing are needs to be fully utilized in mushroom breeding because it is an ideal way to develop the superior strains of Pleurotus.

Intergeneric Transfer of Isolated Nuclei from Lentinus edodes into Protoplasts of Pleurotus florida (사철느타리버섯 원형질체내에 표고 핵의 전이)

  • Yoo, Young-Bok;Shin, Pyung-Gyun
    • The Korean Journal of Mycology
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    • v.24 no.2 s.77
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    • pp.111-126
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    • 1996
  • Transfer of the isolated nuclei from Lentinus edodes into protoplasts of Pleurotus florida was induced with polyethlene glycol (PEG) and $CaCl_2$. The intergeneric transfer products were classified into nuclear hybrid, heterokaryon or synkaryon, and reconstituted cell. These progenies except nuclear hybrids formed mature fruiting bodies on sawdust rice bran medium. Formation of fruit bodies was influenced by several factors such as light, temperature, nutrition and physic state of the culture media. Most of fruiting body characters were similar to those of P. florida in synkaryon and L. edodes in reconstituted cell, respectively. All these basidiocarps had clamp connections though initial heterokaryon colonies were lacking. Isozyme patterns of intergeneric progenies were quite different from those of parents. DNA polymorphisms of transfer products were also compared by random amplified polymorphic DNAs (RAPD) analysis based on polymerase chain reaction. The RAPD patterns were different from those of donor and recipient. DNA fingerprints ranged in size from 0.25 to 4.0 Kb. On the basis of RAPD, the transfer products were classified into five groups. Two synkaryon were analysed with distribution of progenies and segregation of genetic markers by random spore analyses. The genetic markers were segregated into wild type and riboflavine requiring auxotrophs.

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Adaptive and Robust Aeroelastic Control of Nonlinear Lifting Surfaces with Single/Multiple Control Surfaces: A Review

  • Wang, Z.;Behal, A.;Marzocca, P.
    • International Journal of Aeronautical and Space Sciences
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    • v.11 no.4
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    • pp.285-302
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    • 2010
  • Active aeroelastic control is an emerging technology aimed at providing solutions to structural systems that under the action of aerodynamic loads are prone to instability and catastrophic failures, and to oscillations that can yield structural failure by fatigue. The purpose of the aeroelastic control among others is to alleviate and even suppress the vibrations appearing in the flight vehicle subcritical flight regimes, to expand its flight envelope by increasing the flutter speed, and to enhance the post-flutter behavior usually characterized by the presence of limit cycle oscillations. Recently adaptive and robust control strategies have demonstrated their superiority to classical feedback strategies. This review paper discusses the latest development on the topic by the authors. First, the available control techniques with focus on adaptive control schemes are reviewed, then the attention is focused on the advanced single-input and multi-input multi-output adaptive feedback control strategies developed for lifting surfaces operating at subsonic and supersonic flight speeds. A number of concepts involving various adaptive control methodologies, as well as results obtained with such controls are presented. Emphasis is placed on theoretical and numerical results obtained with the various control strategies.

Anaerobic Treatment of Piggery Slurry - Review -

  • Chynoweth, D.P.;Wilkie, A.C.;Owens, J.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.4
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    • pp.607-628
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    • 1999
  • The swine waste industry is growing rapidly along with the world human population. The trend is toward more concentrated piggeries with numbers of herds in the thousands. Associated with these increased herds are large quantities of wastes, including organic matter, inorganic nutrients, and gaseous emissions. The trend in swine waste management is toward treatment of these wastes to minimize negative impact on the health and comfort of workers and animals and the atmosphere, water, and soil environments. Treatment of these wastes has traditionally involved land application, lagoons, oxidation ditches, and conventional batch and continuously stirred reactor designs. More sophisticated treatment systems are being implemented, involving advanced anaerobic digester designs, integrated with solids separation, aerobic polishing of digester effluents, and biological nutrient removal. This review discusses the present and future role of anaerobic processes in piggery waste treatment with emphasis on reactor design, operating and performance parameters, and effluent processing.