• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1527-1532
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• 2016

Strain SPF4211, having hyaluronidase (HAase) inhibition activity, was isolated from P. davidiana (Carriere) Franch fruit (PrDF) sugar extract. The phenotypic and biochemical properties based on 16S rDNA sequencing and an API 50 CHB kit suggested that the organism was B. subtilis. To optimize the HAase inhibition activity of PrDF extract by fermentation of strain SPF4211, a central composite design (CCD) was introduced based on three variables: concentration of PrDF extract (X₁: 1-5%), amount of starter culture (X₂: 1-5%), and fermentation time (X₃: 0-7 days). The experimental data were fitted with quadratic regression equations, and the accuracy of the equations was analyzed by ANOVA. The statistical model predicted the highest HAase inhibition activity of 37.936% under the optimal conditions of X₁ = 1%, X₂ = 2.53%, and X₃ = 7 days. The optimized conditions were validated by observation of an actual HAase inhibition activity of 38.367% from extract of PrDF fermented by SPF4211. These results agree well with the predicted model value.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.438-444
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• 2011

This study was conducted to investigate temperature effect on seed germination behaviors and seed viability changes by drying periods of three Ulmus species (Ulmus davidiana var. japonica (Rehder) Nakai, U. pumila L., and U. parvifolia Jacq.) distributed in Korea. Statistical analysis showed that temperature had a significant effect on mean germination time in three Ulmus species, but its effect on germination percentage was only shown in U. parvifolia (p<0.01). U. davidiana var. japonica and U. pumila germinated relatively consistent in all temperature conditions, especially the latter showed high germination behaviors in low temperatures (10-15$^{\circ}C$). U. parvifolia germinated well in high temperature (25$^{\circ}C$), while this species rarely germinated in low temperature conditions (10-20$^{\circ}C$). The results confirm that seeds of U. pumila and U. parvifolia are orthodox seeds which can be stored under 4-8% moisture condition, and U. davidiana var. japonica seed is recalcitrant seed which is difficult to store for a long time. In conclusion, these differences of the germination behaviors for three Ulmus species seem to be adaptive regimes of their natural habitats, respectively.

• The Korean Journal of Ecology
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• v.13 no.4
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• pp.285-295
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• 1990

Floristic composition and soil properties were analyzed in red pine($Pinus densiflora$) communities in the limestone areas. Tanyang, Ch ungbuk Province in Korea. The tree layer was composed of $Juniperus rigida, Quercus dentata, Q. mongolica$ and others as well as $P. densiflora$, all of which also appeared in soils of grinite origin The shrub layer was dominated by $Q. dentata, Lespedeza cyrtobotrya, Securinega suffruticosa, Abelia coreana$ and $Uimus davidiana$ for $suberosa$. Among these $A. coreana$ and $U. davidiana$ for $suberosa$ belonged to calcicole plant. The herb layer dominated by $Carex lanceolata$. Soil pH ranged 7.9-8.3. Total nitrogen and available phosphorus content of the soil were lower than those of noncalcareous soils. However, exchangeable calcium and magnesium content were 10 times and 2~3 times greater than those of noncalcreous soils, respectively. The difference of soil texture between top soil(loam soil) and subsoil(sand soil), and higher soil organic matter content than noncalcareous red pine forest soils seemed related to the casts forming activities of earthworms.

• Journal of Korean Society of Forest Science
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• v.87 no.2
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• pp.153-163
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• 1998

Genetic relationships of some poplars in the section Leuce, including 5 species and 11 clones of Populus alba${\times}$glandulosa, were investigated on the basis of RAPD marker analysis. Twenty-two of the 88 arbitrary 10-mer primers, showed reproducible amplification in the preliminary experiment with 6 samples, were used for PCR and generated a total of 181 RAPD markers. Genetic relationships among the analyzed samples were tested by two phenetic methods of the UPGMA and the neighbor-joining, which revealed the close genetic relationship between P. glandulosa and P. alba. And the close genetic relationship between P. glandulosa and P. davidiana was ascertained by the principal component analysis. Based on the observation of the close genetic relationship between them, it was deduced that P. glandulosa might be originated by the saltational speciation caused by the hybridization between P. alba and P. davidiana in nature.

• Journal of Life Science
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• v.20 no.2
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• pp.281-291
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• 2010

We investigated the antioxidant effects of hederagenin 3-O-b-D-glucopyranosyl($1{\rightarrow}3$)-a-L-rhamnopyranosyl($1{\rightarrow}2$)-a-L-arabinopyranoside (HDL) isolated from root bark of Ulmus davidiana on the activity of enzymes related to reactive oxygen species (ROS) in human osteosarcoma U2OS cells. Cobalt chloride ($CoCl_2$), a transition metal, was used as an inducer of oxidative stress, generating hydrogen peroxide ($H_2O_2$) via increasing xanthine oxidase (XO) activity. The increased levels of $H_2O_2$, XO, ferritin, and ferritin iron by $CoCl_2$ were diminished effectively by co-treatment with HDL in U2OS cells. Furthermore, decreased levels of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) by $CoCl_2$ were highly increased by co-treatment with HDL in U2OS cells; however, the levels of glutathione peroxidase (GPx) did not change. The increased contents of TBARS related to lipid peroxidation were significantly reduced by HDL in U2OS cells. The concentration of GSH changed in a pattern that went against regulated TBARS by $CoCl_2$ and HDL. We examined the expression of p53, $p21^{CIP1/WAF1}$, and $p27^{KIP1}$ proteins related to oxidative stress and cell cycle regulation. As a result, the expression of $p27^{KIP1}$ modulated by $CoCl_2$ was not changed by HDL. However, the expression of p53 and $p21^{CIP1/WAF}$ increased by $CoCl_2$ was reduced by HDL in U2OS cells. Together with alteration of p53 and $p21^{CIP1/WAF1}$ proteins, the accumulated cells at G1 phase by $CoCl_2$ was decreased by HDL in U2OS cells. Our data suggests that HDL inhibits $CoCl_2$-generated ROS in U2OS cells, providing potentially new antioxidant compounds that are isolated from natural products.

• Journal of Acupuncture Research
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• v.24 no.6
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• pp.15-27
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• 2007

Objectives : The purpose of this study is to investigate the effectiveness of Ulmus davidiana Planch herbal acupuncture(UA) to inhibit nuclear $factor(NF)-{\kappa}B$ activation on type II collagen-induced arthritis (CIA) in mice. Methods : Using an in vitro test, the synoviocytes picked out from the experimental CIA mice were subcultured. The synoviocyte cells were treated with phorbol-12-myristate-13-acetate(PMA) for 1 hour prior to the addition of indicated concentrations($0.4\;-\;1.0mg/m{\ell}$) of UA, and the cells were further incubated for 24 hours. The in vivo test, $NF-{\kappa}B$ p65, inducible nitric oxide synthase(iNOS), cyclooxygenase-2(COX-2), vascular cell adhesion molecule(VCAM)-1 production and apoptosis was observed by immunohistochemical staining. Results : The PMA-induced $I{\kappa}B$ kinase(IKK), iNOS and COX-2 mRNA expression were dose-dependently decreased in UA treated synoviocytes. Using the in vivo test, the number of eosinophils in mice treated with UA noticeably decreased in the the CIA group(P<0.05 using student t test). In mice treated with UA, there was less cartilage erosion. less bone destruction, mild synovial hyperplasia, mild fibrosis, and mild angiogenesis with less MIP-2 production. By immunohistochemical staining, suppression of $NF-{\kappa}B$ p65, iNOS production, inhibition of COX-2 production, inhibition of VCAM-1 production and inducing apoptosis were observed. Conclusions : These results suggest that UA might be applicable to the therapy of RA to suppress $NF-{\kappa}B$ activation.

• Korean Journal of Environment and Ecology
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• v.27 no.6
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• pp.733-744
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• 2013

This study was launched in order to collect basic data such as emergent species, a succession trend based on the actual vegetation at the ridge from Haneoryoung to Daetjae, thereby designating 46 quadrats($10m{\times}10m:100m^2$). As a result of classification by TWINSPAN, the plant community was categorized into eight different communities, such as Pinus densiflora-Quercus mongolica-Fraxinus rhynchophylla, P. densiflora-Q. mongolica, Ulmus davidiana var. japonica, Q. mongolica-Deciduous Broad-leaved, Q. mongolica, Q. mongolica-Betula davurica, Q. mongolica-F. sieboldiana, and Larix kaempferi. An analyzation of each communities indicated that P. densiflora dominant community will change into a Q. mongolica dominant community if there are no outside artificial disturbances. U. davidiana var. japonica community maintains its present condition, because it doesn't appear to be a competitive species. In the case of Q. mongolica dominant community, if nothing comes in a competitive species against Q. monglica, it is possible to succeed to a muture stand. These communities with competitive species such as F. rhynchophylla, B. davurica that do not emerge at a canopy layer will gradually progress to the Deciduous Broad-leaved community. With regard to the species diversity index per unit ($400m^2$), succession processing communities (P. densiflora: 1.0477~1.1283) tend to be higher than succession-processed communities (Q. mongolica dominant: 0.6446~0.9424).

• Journal of Acupuncture Research
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• v.22 no.2
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• pp.93-101
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• 2005

Objective: We have evaluated UDHA into the joint for its effectiveness on immune responses to CII in the rat CIA. In an attempt to gain further insight into the mode of action of UDHA, we also investigated the effects of UDHA on the incidence and development of arthritis in rat CIA with 2 different regimens: (1) started prior to a primary immunization, (2) started on the day of a primary immunization. Methods : Male rats were immunized with an emulsion of $200\;{\mu}g/100g$ of CII and complete Freund's adjuvant (CFA). The rats were then given intraperitoneal(i.p) stimulation of Ulmus davidiana Planch herbal acupuncture(UDHA) or saline during the experiment. Lymph node cells were obtained from rats 14 days after immunization and cultured in vitro with CII. When compared with rats treated with saline as control, UDHA at doses of more than $20\;{\mu}g/100\;g$ rat once a day for 7 days inhibited the ability of inguinal lymph node cells to produce T cell cytokines interleukin-$1{\beta}$, tumor necrosis $factor-{\alpha}$ $(TNF-{\alpha})$. When rats were injected intraperitoneally with SRBC, hemaglutination titers in UD-treated and control rats did not differ significantly when low doses of UD was given to rats. However, i.p injection of UD at doses of more than $10\;{\mu}g/100\;g/day$ for 7 days slightly suppressed antibody production. Results : The present results show that treatment with UDHA can inhibit the onset and development of arthritis and the immune responses to collagen. Conclusion: Therapeutic i.p injection with UD affect the clinical course of the disease and the immune responses to CII.

• Journal of Korean Society of Forest Science
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• v.105 no.3
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• pp.309-314
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• 2016

A locally adapted plant population under harsh environmental changes might survive for a long generation through maintaining proper level of genetic diversity. When it happens losing the genetic diversity too much fast, the population could be declining and probably become extinct. An isolated small population of Populus davidiana was investigated to study out the genetic diversity and the fine-scale spatial genetic structure. The estimated number of adult trees in the population of Mt. Worak, South Korea, was 350 in the total area of $14,000m^2$. The number of adults in a study plot ($70m{\times}70m$) was 123. The average age was 16-year-old and a 32-year-old tree was the oldest. The distribution of individuals was slightly aggregated in the plot. Sixty-one among the 123 individuals were randomly sampled to estimate genetic variation using AFLP markers. One hundred fifty-one (77%) of total 196 amplicons were polymorphic from six AFLP primer combinations. The average number of loci per primer combination was 32.7 (S.D.=7.2). Expected heterozygosity ($H_e$) and Shannon's diversity index (S.I.) were 0.154 and 0.254, respectively. These values were extremely lower than those of other P. davidiana populations in South Korea. Genetic patchiness was showed within 21 meters by spatial autocorrelation analysis and the isolated small size of population might be mainly attributed to the formation of such small patch size.

• Journal of Korean Society of Forest Science
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• v.95 no.4
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• pp.443-452
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• 2006

This study was conducted to investigate the site and growth characteristics of Mackia amurensis stand in natural forest of Mt. Joongwang, Pyongchang-Gun, Gangwon-Do, Korea. The 28 sample plots ($30m{\times}30m$) were established to examine the vegetation and site characteristics for M. amurensis stand. Stem analysis was performed on two sample trees for each of the 5 plots situated at intervals of 100 m by altitude. M. amurensis trees were mainly distributed at the ridge and the slope facing north, with altitudes ranging from 790 to 1,170 m. The M. amurensis grew at relatively deep A-layer of soil with high moisture but infertile. Dominant species with M. amurensis were Quercus mongolica, Fraxinus rhynchophylla, Acer pseudo-sieboldianum, A. pictum subsp. mono, Ulmus davidiana var., japonica, Tilia amurensis, and Populus davidiana. TWINSPAN classified four communities in Mt. Joongwang area; M. amurensis-P. davidiana community (xeric type), M. amurensis-U. davidiana var. japonica community (xeric type), M. amurensis-T. amurensis community (moist type), and M. amurensis-Styrax obassia community (moist type). According to the community type, trees per ha were more abundant in the area of the moist types than the xeric types. Height growth of M. amurensis increased with increasing ages until 10 years, and then decreased thereafter, especially at altitude of 1,020m. M. amurensis showed rapid early growth of height and volume, indicating possible high biomass production under enough light. Growth of M. amurensis increased greatly at about 40 years if released from severe competitions. The rotation age of natural M. amurensis stand was expected for above 50 years.