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http://dx.doi.org/10.14578/jkfs.2016.105.3.309

Fine-scale Spatial Genetic Structure of a Small Natural Stand of Populus davidiana in South Korea using AFLP markers  

Lee, Min Woo (Forest Genetic Resources Division, National Institute of Forest Science)
Hong, Kyung Nak (Forest Genetic Resources Division, National Institute of Forest Science)
Park, Yu Jin (Forest Genetic Resources Division, National Institute of Forest Science)
Lee, Jei Wan (Forest Genetic Resources Division, National Institute of Forest Science)
Lim, Hyo In (Forest Genetic Resources Division, National Institute of Forest Science)
Publication Information
Journal of Korean Society of Forest Science / v.105, no.3, 2016 , pp. 309-314 More about this Journal
Abstract
A locally adapted plant population under harsh environmental changes might survive for a long generation through maintaining proper level of genetic diversity. When it happens losing the genetic diversity too much fast, the population could be declining and probably become extinct. An isolated small population of Populus davidiana was investigated to study out the genetic diversity and the fine-scale spatial genetic structure. The estimated number of adult trees in the population of Mt. Worak, South Korea, was 350 in the total area of $14,000m^2$. The number of adults in a study plot ($70m{\times}70m$) was 123. The average age was 16-year-old and a 32-year-old tree was the oldest. The distribution of individuals was slightly aggregated in the plot. Sixty-one among the 123 individuals were randomly sampled to estimate genetic variation using AFLP markers. One hundred fifty-one (77%) of total 196 amplicons were polymorphic from six AFLP primer combinations. The average number of loci per primer combination was 32.7 (S.D.=7.2). Expected heterozygosity ($H_e$) and Shannon's diversity index (S.I.) were 0.154 and 0.254, respectively. These values were extremely lower than those of other P. davidiana populations in South Korea. Genetic patchiness was showed within 21 meters by spatial autocorrelation analysis and the isolated small size of population might be mainly attributed to the formation of such small patch size.
Keywords
Populus davidiana; genetic diversity; spatial genetic structure; AFLP; genetic patch; spatial autocorrelation;
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