• 한국정보통신학회논문지
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• 제9권1호
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• pp.95-101
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• 2005

본 논문에서는 모바일 에드혹 네트워크에서 멀티미디어 트래픽의 전송특성을 시뮬레이션으로 연구하였다. 시뮬레이션에서는 멀티미디어 트래픽으로 MPEG 비디오 형식의 합성 스트리밍 비디오를 사용하였으며, 합성 스트리밍 비디오는 비디오 스티림 알고리즘을 사용하여 생성하였다. 비디오 합성 알고리즘은 I(intra-coded), P(predicted-coded), B(bidirectional-coded) 프레임 열로 구성되는 특정 GOP(group of pictures) 패턴을 사용하여 MPEG 비디오 스트림에 대응하는 VBR 트래픽을 생성한다. 이 합성 VBR 스트림을 모바일 애드혹 네트워크 상에서 UDP 프로토콜을 사용하여 전송하였으며, 라우팅 프로토콜로는 AODV와 DSR을 사용하였다. 모바일 에드혹 네트워크의 비디오 스트림 전송성능으로서 패킷지연, 패킷전달율 및 수율을 분석하였으며, 데이터 트래픽과 비디오 트래픽의 전송수율을 비교하여 보았다.

• 정보과학회 논문지
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• 제43권3호
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• pp.327-335
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• 2016

본 논문에서는 실감교류 멀티미디어 서비스에 적합한 버퍼 관리 방안을 제안한다. 수신 버퍼 크기가 왕복 시간 추정에 따라 달라질 수 있도록 전형적인 지연 최적화 환경을 고려한다. 이러한 환경에서, 버퍼 크기 단축 시 버퍼 내에 I/P/B 프레임을 드롭하는 경우 발생할 수 있는 정보 손실을 최소화하기 위한 최적화 기법을 제안한다. 근사 해를 찾기 위해 동적 프로그래밍을 이용하는 Knapsack Problem으로 문제를 모델링한다. 제안된 기법은 기존의 버퍼 관리 기법과 비교된다. 시뮬레이션 연구를 통해, 제안하는 접근 방식은 비디오 품질에 중요한 PSNR을 증가시킬 수 있음을 확인하였다.

• ALGAE
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• 제33권1호
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• pp.49-54
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• 2018

Red algal mitochondrial genomes (mtDNAs) can provide useful information on species identification. mtDNAs of Pyropia / Porphyra (Bangiales, Rhodophyta) have shown diverse variation in their size and gene structure. In particular, the introns and intronic open reading frames found in the ribosomal RNA large subunit gene (rnl) and cytochrome c oxidase subunit 1 gene (cox1) significantly vary the mitochondrial genome size in Pyropia / Porphyra species. In this study, we examined the exon / intron structure of rnl and cox1 genes of Pyropia yezoensis at the intraspecific level. The combined data of rnl and cox1 genes exhibited 12 genotypes for 40 P. yezoensis strains, based on the existence of introns. These genotypes were more effective to identify P. yezoensis strains in comparison to the traditional DNA barcode cox1 marker (5 haplotypes). Therefore, the variation in gene structure of rnl and cox1 can be a novel molecular marker to discriminate the strains of Pyropia species.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.593-598
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• 2004

The arsenical resistance (ars) operon was cloned from a 67-kilobase pair (kb) plasmid, which was previously shown to be responsible for arsenic salts resistance in K. oxytoca D12. When plasmid pAE48, carrying the ars operon, was transformed into E. coli, transformed cells displayed enhanced survival in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. The nucleotide sequence of the 5.6-kb fragment encoding arsenical resistance revealed five open reading frames (ORFs), which were predicted to encode polypeptides of 12.8 (arsR), 13.4 (arsD), 62.6 (arsA), 45 (arsB), and 16.7 (arse) kilodaltons (kDa). Each ORF was preceded by a ribosome binding site. A putative promoter-like sequence was identified upstream of arsR, and a possible termination site was found downstream of arsC. When the deduced amino acid sequences of the K. oxytoca Dl2 Ars proteins were compared with the amino acid sequences of the E. coli R773 Ars proteins, a significant amino acid similarity was observed (87.9％ for ArsR, 89.2％ for ArsD, 83.2％ for ArsA, 92.6％ for ArsB, and 91.3％ for ArsC), suggesting an evolutionary relationship of the ars genes of E. coli plasmid R773 and K. oxytoca Dl2.

• 복합신소재구조학회 논문집
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• 제2권4호
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• pp.28-34
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• 2011

본 연구는 인공지진파 및 기록 지진파를 이용하여, KBC2009 규준으로 설계된 강구조 건물의 거대 건물에 대한 내력 여유도를 평가하는 것을 목표로 하고 있다. 이 논문에서는 검정에 있어서 콘크리트 슬래브로 층강성이 고정되어 있는 2-D 프레임을 고려하였고, 각각의 프레임을 구성하고 있는 보와 기둥 부재는 각 부재단에 소성힌지를 적용하였다. 검정에 사용한 해석법은 응답 스펙트럼을 이용한 모드 해석과 기록 및 인공지진파를 이용한 시간이력해석을 선택하여 모델의 거동을 조사하였으며 해석에서는 P-delta 효과를 고려한다.

• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.870-875
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• 2001

A DNA fragment, pCKB13, containing two genes encoding Coenzyme a transferase, was isolated from a genomic DNA library of S. marcescens KCTC 2172. The complete nucleotide sequence of the 2,081-bp BamHI fragment on pCKB13 was determined. Sequencing of the fragment led to the identification of two open reading frames showing high homology with two Coenzyme A (CoA) transferases, Acetoacetyl-CoA transferase (Acot) and Succinyl-CoA transferase (Scot), enzymes catalyzing the reversible transfer of CoA from one carboxylic acid to another. The enzyme activity of Coenzyme A transferase increased after introducing the multicopy of the cloned gene in E. coli. The recombinant protein, overexpressed by multicopy and induction with IPTG, was a polypeptide of 42 kDa, as confirmed by SDS-PAGE. The protein was purified to homogeneity through three sequential chromatographic procedures including ion-exchanged DEAE-sepharose, CM-sepharose, and Mono Q.

• Molecules and Cells
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• 제40권10호
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• pp.796-804
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• 2017

Endogenous retroviruses (ERVs) have been integrated into vertebrate genomes and have momentously affected host organisms. Horses (Equus caballus) have been domesticated and selected for elite racing ability over centuries. ERVs played an important role in the evolutionary diversification of the horse genome. In the present study, we identified six equine ERV families (EqERVs-E1, I1, M2, P1, S1, and Y4), their full-length viral open reading frames (ORFs), and elucidated their phylogenetic relationships. The divergence time of EqERV families assuming an evolutionary rate of 0.2%/Myr indicated that EqERV-S3 (75.4 million years ago; mya) on chromosome 10 is an old EqERV family and EqERV-P5 (1.2 Mya) on chromosome 12 is a young member. During the evolutionary diversification of horses, the EqERV-I family diverged 1.7 Mya to 38.7 Mya. Reverse transcription quantitative real-time PCR (RT-qPCR) amplification of EqERV pol genes showed greater expression in the cerebellum of the Jeju horse than the Thoroughbred horse. These results could contribute further dynamic studies for horse genome in relation to EqERV gene function.

• The Plant Pathology Journal
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• 제31권1호
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• pp.83-89
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• 2015

In order to control Pectobacterium carotovorum subsp. carotovorum, a novel virulent bacteriophage PM2 was isolated. Bacteriophage PM2 can infect 48% of P. carotovorum subsp. carotovorum and 78% of P. carotovorum subsp. brasilliensis but none of atrosepticum, betavasculorum, odoriferum and wasabiae isolates had been infected with PM2. PM2 phage belongs to the family Myoviridae, and contains a large head and contractile tail. It has a 170,286 base pair genome that encodes 291 open reading frames (ORFs) and 12 tRNAs. Most ORFs in bacteriophage PM2 share a high level of homology with T4-like phages including IME08, RB69, and JS98. Phylogenetic analysis based on the amino acid sequence of terminase large subunits confirmed that PM2 is classified as a T4-like phage. It contains no integrase- or no repressor-coding genes related to the lysogenic cycle, and lifestyle prediction using PHACT software suggested that PM2 is a virulent bacteriophage.

• Journal of Microbiology
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• 제35권1호
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• pp.40-46
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• 1997

The pWHM220 cosmid with a 24-kb insert cloned from Streptomyces albus ATCC 21838 induces the biosynthesis of a polysther antibiotic similar to salinomycin in Streptomyces invidans. We have analyzed this region by DNA sequencing as well as Southern blot hybridization with type I and type II polyketide synthase (PKS) probes. Surprisingly, we found another set of type II SKS genes only 10-kb from the original PKS genes, salABCDE. The DNA sequence revealed two complete open reading frames (ORFs) named salB2 and salC2, and one partial ORF that does not resemble any known DNA or deduced protein sequence. The salC2 should code for chain length determining factor while the deduced amino acid sequence encoded by salB2 exhibits high similarity to .betha.-ketoacyl synthase from different PKS gene clusters. The highest identity was found for .betha.-keetoacyl synthases from S. argillaceus (MtmP. 59.1% identity), the mithramycin producer and from S. venezuelae ISP5230 (JadA, 52.3% identity), the jadomycin producer. The SalC2 protein clearly resembles its counterparts in order aromatic PKS gene clusters that are believed to influence the length of the polyketide chain. The highest identities observed were to that of S. argillaceus (MtmK, 62.3%) and S. venezuelae ISP 5230 (JadB, 55.1%) proteins, Moreover, the deduced amino acid sequences of the salB2 and salC2 products were 29.0% identical.

• Structural Engineering and Mechanics
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• 제33권6호
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• pp.725-745
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• 2009

In this paper, probabilistic seismic performance assessment of a typical non-seismic RC frame building representative of a large inventory of existing buildings in developing countries is conducted. Nonlinear time-history analyses of the sample building are performed with 20 large-magnitude medium distance ground motions scaled to different levels of intensity represented by peak ground acceleration and 5% damped elastic spectral acceleration at the first mode period of the building. The hysteretic model used in the analyses accommodates stiffness degradation, ductility-based strength decay, hysteretic energy-based strength decay and pinching due to gap opening and closing. The maximum inter story drift ratios obtained from the time-history analyses are plotted against the ground motion intensities. A method is defined for obtaining the yielding and collapse capacity of the analyzed structure using these curves. The fragility curves for yielding and collapse damage levels are developed by statistically interpreting the results of the time-history analyses. Hazard-survival curves are generated by changing the horizontal axis of the fragility curves from ground motion intensities to their annual probability of exceedance using the log-log linear ground motion hazard model. The results express at a glance the probabilities of yielding and collapse against various levels of ground motion intensities.